Last updated: 2020-09-24

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Knit directory: misc/

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Rmd 0fb979b DongyueXie 2020-09-24 wflow_publish(“analysis/rnavelocity.Rmd”)

RNA velocity is the time derivative of the gene expression state. It can be directly estimated by distinguishing between unspliced and spliced mRNAs in common single-cell RNA sequencing protocols.

Key equation: the first time derivative of the spliced mRNA abundance (RNA velocity) is determined by the balance between production of spliced mRNA from unspliced mRNA, and the mRNA degradation, \(\frac{ds}{dt} = u-\gamma*s\).

This simple model can be used to extrapolate the mature mRNA abundance into the future