Last updated: 2020-02-19

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Knit directory: misc/

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Rmd 460fe4b Dongyue Xie 2020-02-19 wflow_publish(“analysis/GTEX.Rmd”)
html b85c283 Dongyue Xie 2020-01-15 Build site.
Rmd 605578a Dongyue Xie 2020-01-15 wflow_publish(“analysis/GTEX.Rmd”)

Introduction

Number of SRA RNA seq files:

Heart - 369

Brain - 1055

Lung - 242

Muscle - 330

Nerve - 238

Thyroid - 250

for i in {SRR1069802,SRR1071692,SRR1072650,SRR1075631,SRR1075997,SRR1076779,SRR1077115,SRR1077528,SRR1077643};

do 

prefetch $i;
rm *.1;
rm *.10;
rm *.11;
rm *.9;
rm *.13;
rm *.8;

done

An easier one(could try later):

cat your_accession_list.txt | while read i
do  
fasterq-dump ${i} <options>
done

try for loop…too slow

for i in {SRR1069690,SRR1069802,SRR1071692,SRR1072650,SRR1075631,SRR1075997,SRR1076779,SRR1077115,SRR1077528,SRR1077643};

do

sam-dump $i.sra | samtools view -Sb > /project2/mstephens/dongyue/gtex/$i.bam ;

done

try the parallel version

find *.sra | parallel 'sam-dump {} | samtools view -Sb > /project2/mstephens/dongyue/gtex/{/.}.bam'

sessionInfo()
R version 3.6.1 (2019-07-05)
Platform: x86_64-apple-darwin15.6.0 (64-bit)
Running under: macOS High Sierra 10.13.6

Matrix products: default
BLAS:   /Library/Frameworks/R.framework/Versions/3.6/Resources/lib/libRblas.0.dylib
LAPACK: /Library/Frameworks/R.framework/Versions/3.6/Resources/lib/libRlapack.dylib

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base     

loaded via a namespace (and not attached):
 [1] workflowr_1.5.0 Rcpp_1.0.2      rprojroot_1.3-2 digest_0.6.21  
 [5] later_1.0.0     R6_2.4.0        backports_1.1.5 git2r_0.26.1   
 [9] magrittr_1.5    evaluate_0.14   stringi_1.4.3   rlang_0.4.0    
[13] fs_1.3.1        promises_1.1.0  whisker_0.4     rmarkdown_1.16 
[17] tools_3.6.1     stringr_1.4.0   glue_1.3.1      httpuv_1.5.2   
[21] xfun_0.10       yaml_2.2.0      compiler_3.6.1  htmltools_0.4.0
[25] knitr_1.25