Last updated: 2022-02-10

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Rmd d199bd4 Belinda Cornes 2022-02-10 QC analysis

Genotype Summary by Sample

Neogen_Sample_ID Missing AA AC AG AT CC CG GC GG TA TC TG TT Total_Genotyped Percent_Genotyped MURGIGV01_SNP.Count cr
NG00205-PBS 9843 28794 1617 6791 7 29496 4 1 29812 2 6702 1600 28590 133416 93.13% 143259 0.931292274830901
NG00686-PBS 14726 26500 2034 8688 7 26988 6 4 27506 3 8760 1961 26076 128533 89.72% 143259 0.897207156269414
NG00779-ICI 5230 31232 1077 4492 7 32257 4 6 32538 4 4357 1067 30988 138029 96.35% 143259 0.963492695048828
NG00305-EOI 5276 29979 1539 6385 3 31019 2 3 31373 3 6322 1553 29802 137983 96.32% 143259 0.963171598293999
NG01034-ICI 14236 25997 1846 8284 10 28478 7 4 28938 4 8177 1790 25488 129023 90.06% 143259 0.900627534744763
NG00499-EOI 12769 26332 1833 8470 8 28741 7 5 29094 3 8338 1748 25911 130490 91.09% 143259 0.910867729078103
NG00246-PBS 5230 30749 1348 5260 5 31628 7 3 32015 4 5295 1241 30474 138029 96.35% 143259 0.963492695048828
NG00241-ICI 5246 30140 1521 6274 10 31099 5 5 31402 2 6196 1426 29933 138013 96.34% 143259 0.963381009221061
NG00796-ICI 6562 27873 2198 9230 12 29124 9 4 29437 4 9092 2138 27576 136697 95.42% 143259 0.954194849887267
NG00309-EOI 8459 28070 1940 8167 5 29314 6 3 29620 2 8023 1859 27791 134800 94.10% 143259 0.940953098932702
NG00872-ICI 5276 29474 1835 7305 10 30418 8 5 30688 5 7208 1720 29307 137983 96.32% 143259 0.963171598293999
NG00530-EOI 5327 29967 1656 6495 7 30887 5 4 31189 1 6354 1587 29780 137932 96.28% 143259 0.962815599717993
NG00290-PBS 5210 30287 1467 6098 5 31213 6 5 31492 3 6099 1453 29921 138049 96.36% 143259 0.963632302333536
NG00249-ICI 5260 29579 1733 7071 8 30641 6 5 30929 1 6961 1647 29418 137999 96.33% 143259 0.963283284121765
NG00797-ICI 5187 28824 1973 8357 11 29937 9 4 30182 2 8124 1967 28682 138072 96.38% 143259 0.96379285071095
NG00310-EOI 5166 29231 1842 7760 5 30194 4 3 30486 3 7597 1831 29137 138093 96.39% 143259 0.963939438359894
NG00974-ICI 5168 28670 2083 8654 10 29676 8 6 29967 3 8489 2022 28503 138091 96.39% 143259 0.963925477631423
NG00532-EOI 5148 29807 1692 6771 4 30747 5 3 31123 5 6710 1634 29610 138111 96.41% 143259 0.964065084916131
NG00298-PBS 5164 28947 1968 8048 11 30123 7 4 30373 1 7932 1881 28800 138095 96.40% 143259 0.963953399088364
NG00494-ICI 5209 30027 1516 6303 8 31172 2 3 31480 3 6215 1483 29838 138050 96.36% 143259 0.963639282697771
NG00877-ICI 5176 30273 1418 5914 12 31394 5 3 31682 3 5910 1413 30056 138083 96.39% 143259 0.96386963471754
NG00324-EOI 5164 29631 1667 7068 9 30757 1 1 30946 2 6955 1698 29360 138095 96.40% 143259 0.963953399088364
NG00923-ICI 5180 31047 1247 4920 7 31906 5 4 32190 2 4915 1193 30643 138079 96.38% 143259 0.963841713260598
NG00534-EOI 5204 29082 1921 7806 8 30130 5 5 30487 5 7643 1853 29110 138055 96.37% 143259 0.963674184518948
NG00432-PBS 5106 29579 1767 7159 10 30578 6 5 30886 3 7138 1732 29290 138153 96.44% 143259 0.964358260214018
NG00498-ICI 5158 28447 2213 9004 9 29441 5 4 29737 4 8827 2149 28261 138101 96.40% 143259 0.963995281273777
NG00852-ICI 5178 30443 1390 5758 8 31522 7 4 31771 2 5541 1337 30298 138081 96.39% 143259 0.963855673989069
NG00316-EOI 5176 29512 1790 7208 4 30437 3 5 30844 3 7162 1758 29357 138083 96.39% 143259 0.96386963471754
NG00920-ICI 12058 28463 1335 5965 7 29863 3 4 30349 2 5774 1325 28111 131201 91.58% 143259 0.915830768049477
NG00525-EOI 12088 27448 1903 8181 10 28124 4 1 28497 0 8051 1829 27123 131171 91.56% 143259 0.915621357122415
NG00507-PBS 10241 27798 1758 7543 10 29375 6 5 29810 2 7623 1762 27326 133018 92.85% 143259 0.928514089865209
NG00524-ICI 13335 26576 1945 8361 13 28000 6 7 28580 2 8336 1872 26226 129924 90.69% 143259 0.906916842920864
NG00242-EOI 5336 28978 1902 8079 7 29933 5 4 30242 5 8048 1893 28827 137923 96.28% 143259 0.962752776439875
NG00327-EOI 5364 28374 2140 9034 8 29436 4 4 29699 2 8802 2107 28285 137895 96.26% 143259 0.962557326241283
NG00922-ICI 5200 31491 954 4190 8 32419 5 5 32705 0 4042 975 31265 138059 96.37% 143259 0.96370210597589
NG00527-EOI 6810 28669 1871 7610 6 29992 5 3 30390 3 7436 1798 28666 136449 95.25% 143259 0.952463719556886
NG00538-PBS 5304 32202 766 2988 5 33070 2 1 33407 3 2865 680 31966 137955 96.30% 143259 0.962976148095408
NG00551-ICI 5270 29395 1821 7450 9 30371 9 7 30657 2 7305 1750 29213 137989 96.32% 143259 0.963213480479411
NG00218-EOI 5163 30305 1456 5983 8 31385 6 4 31621 3 5734 1417 30174 138096 96.40% 143259 0.9639603794526
NG00351-EOI 5200 29791 1622 6881 8 30871 8 4 31077 2 6597 1568 29630 138059 96.37% 143259 0.96370210597589
NG00985-ICI 5212 29908 1661 6646 10 30869 5 6 31166 2 6487 1557 29730 138047 96.36% 143259 0.963618341605065
NG00565-EOI 5205 28993 2023 8148 8 29944 8 5 30214 2 7959 1948 28802 138054 96.37% 143259 0.963667204154713
NG00548-PBS 5120 29142 1963 7886 9 30130 8 5 30392 2 7759 1923 28920 138139 96.43% 143259 0.964260535114722
NG00577-ICI 5186 30883 1208 5097 9 31842 6 4 32148 5 4995 1209 30667 138073 96.38% 143259 0.963799831075186
NG00269-EOI 5204 30406 1447 5743 4 31393 2 4 31724 1 5646 1429 30256 138055 96.37% 143259 0.963674184518948
NG00388-EOI 5260 29601 1702 7122 11 30602 9 5 30870 1 6950 1684 29442 137999 96.33% 143259 0.963283284121765
NG01021-ICI 5110 29281 1855 7546 8 30378 6 5 30687 6 7414 1820 29143 138149 96.43% 143259 0.964330338757076
NG00566-EOI 5138 29554 1788 7208 6 30548 4 5 30829 3 7135 1741 29300 138121 96.41% 143259 0.964134888558485
NG00561-PBS 5129 28584 2112 8771 7 29716 6 3 29972 4 8493 1992 28470 138130 96.42% 143259 0.964197711836604
NG00630-ICI 5117 28555 2147 8910 8 29525 6 5 29789 3 8820 2093 28281 138142 96.43% 143259 0.964281476207429
NG00293-EOI 5610 29109 1929 7804 7 30012 6 5 30367 3 7682 1823 28902 137649 96.08% 143259 0.960840156639373
NG00389-EOI 5591 29987 1606 6306 6 30924 4 4 31308 3 6241 1510 29769 137668 96.10% 143259 0.960972783559846
NG01022-ICI 5366 30340 1441 5824 10 31249 7 4 31660 1 5869 1364 30124 137893 96.25% 143259 0.962543365512812
NG00567-EOI 5605 29877 1612 6513 5 31007 5 1 31194 3 6327 1546 29564 137654 96.09% 143259 0.96087505846055
NG00586-PBS 5186 30218 1457 6073 6 31295 3 5 31616 1 5860 1440 30099 138073 96.38% 143259 0.963799831075186
NG00662-ICI 5213 29817 1643 6763 7 30759 7 6 31090 2 6733 1603 29616 138046 96.36% 143259 0.96361136124083
NG00294-EOI 5169 30425 1439 5829 7 31417 8 3 31689 2 5693 1370 30208 138090 96.39% 143259 0.963918497267187
NG00396-EOI 5123 29140 1940 7919 9 30150 3 3 30380 1 7710 1884 28997 138136 96.42% 143259 0.964239594022016
NG00971-ICI 5277 30778 1302 5157 4 31727 6 7 32083 5 5125 1264 30524 137982 96.32% 143259 0.963164617929764
NG00647-PBS 5226 30241 1422 6085 5 31324 5 2 31511 2 6035 1483 29918 138033 96.35% 143259 0.963520616505769
NG00606-PBS 5284 29618 1673 7128 10 30593 7 6 30878 5 6981 1591 29485 137975 96.31% 143259 0.963115755380116
NG00698-ICI 5421 30449 1355 5596 5 31497 2 3 31764 1 5532 1391 30243 137838 96.22% 143259 0.962159445479865
NG00296-EOI 5157 29445 1770 7328 9 30557 9 3 30827 0 7108 1665 29381 138102 96.40% 143259 0.964002261638012
NG00450-EOI 5210 29508 1812 7250 9 30444 6 6 30806 5 7145 1708 29350 138049 96.36% 143259 0.963632302333536
NG00959-ICI 5164 30975 1227 4906 5 31946 7 4 32260 2 4849 1194 30720 138095 96.40% 143259 0.963953399088364
NG00673-PBS 5246 29331 1860 7491 6 30355 8 4 30678 1 7361 1805 29113 138013 96.34% 143259 0.963381009221061
NG00638-PBS 5188 29878 1631 6651 8 30895 9 7 31200 5 6465 1620 29702 138071 96.38% 143259 0.963785870346715
NG00777-ICI 5301 31057 1169 4677 9 32077 5 2 32380 4 4566 1112 30900 137958 96.30% 143259 0.962997089188114
NG00302-EOI 5200 30021 1614 6437 11 31035 9 4 31337 0 6273 1503 29815 138059 96.37% 143259 0.96370210597589
NG00451-EOI 5157 30022 1503 6530 8 31068 5 2 31301 2 6354 1462 29845 138102 96.40% 143259 0.964002261638012
NG00497-EOI 7275 27786 2184 9180 12 28838 7 4 29220 3 9162 2069 27519 135984 94.92% 143259 0.949217850187423
NG00674-PBS 8000 29314 1583 6401 8 30311 9 3 30736 2 6358 1527 29007 135259 94.42% 143259 0.944157086116754

Gender Summary by Sample

Neogen_Sample_ID Provided.Sex Inferred.Sex
NG00205-PBS F M
NG00686-PBS F M
NG00779-ICI F M
NG00305-EOI F M
NG01034-ICI F M
NG00499-EOI F M
NG00246-PBS F M
NG00241-ICI F M
NG00796-ICI F M
NG00309-EOI F M
NG00872-ICI F M
NG00530-EOI F M
NG00290-PBS F M
NG00249-ICI F M
NG00797-ICI F M
NG00310-EOI F M
NG00974-ICI F M
NG00532-EOI F M
NG00298-PBS F M
NG00494-ICI F M
NG00877-ICI F M
NG00324-EOI F M
NG00923-ICI F M
NG00534-EOI F M
NG00432-PBS F M
NG00498-ICI F M
NG00852-ICI F M
NG00316-EOI F M
NG00920-ICI F M
NG00525-EOI F M
NG00507-PBS F M
NG00524-ICI F M
NG00242-EOI F M
NG00327-EOI F M
NG00922-ICI F M
NG00527-EOI F M
NG00538-PBS F M
NG00551-ICI F M
NG00218-EOI F M
NG00351-EOI F M
NG00985-ICI F M
NG00565-EOI F M
NG00548-PBS F M
NG00577-ICI F M
NG00269-EOI F M
NG00388-EOI F M
NG01021-ICI F M
NG00566-EOI F M
NG00561-PBS F M
NG00630-ICI F M
NG00293-EOI F M
NG00389-EOI F M
NG01022-ICI F M
NG00567-EOI F M
NG00586-PBS F M
NG00662-ICI F M
NG00294-EOI F M
NG00396-EOI F M
NG00971-ICI F M
NG00647-PBS F M
NG00606-PBS F M
NG00698-ICI F M
NG00296-EOI F M
NG00450-EOI F M
NG00959-ICI F M
NG00673-PBS F M
NG00638-PBS F M
NG00777-ICI F M
NG00302-EOI F M
NG00451-EOI F M
NG00497-EOI F M
NG00674-PBS F M

Phenotype Summary by Sample

Neogen_Sample_ID age.of.onset group diabetic.status strain
NG00205-PBS 34.9 PBS Spont. T1D (F1 x NOD)BC1
NG00686-PBS 15.9 PBS Spont. T1D (F1 x NOD)BC1
NG00779-ICI 14.1 ICI T1D <17 (F1 x NOD)BC1
NG00305-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG01034-ICI 12.4 ICI T1D <17 (F1 x NOD)BC1
NG00499-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00246-PBS 16.4 PBS Spont. T1D (F1 x NOD)BC1
NG00241-ICI 14.0 ICI T1D <17 (F1 x NOD)BC1
NG00796-ICI 13.0 ICI T1D <17 (F1 x NOD)BC1
NG00309-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00872-ICI 13.1 ICI T1D <17 (F1 x NOD)BC1
NG00530-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00290-PBS 28.7 PBS Spont. T1D (F1 x NOD)BC1
NG00249-ICI 11.4 ICI T1D <17 (F1 x NOD)BC1
NG00797-ICI 11.7 ICI T1D <17 (F1 x NOD)BC1
NG00310-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00974-ICI 12.9 ICI T1D <17 (F1 x NOD)BC1
NG00532-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00298-PBS 25.7 PBS Spont. T1D (F1 x NOD)BC1
NG00494-ICI 14.4 ICI T1D <17 (F1 x NOD)BC1
NG00877-ICI 11.3 ICI T1D <17 (F1 x NOD)BC1
NG00324-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00923-ICI 12.9 ICI T1D <17 (F1 x NOD)BC1
NG00534-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00432-PBS 17.3 PBS Spont. T1D (F1 x NOD)BC1
NG00498-ICI 13.4 ICI T1D <17 (F1 x NOD)BC1
NG00852-ICI 12.0 ICI T1D <17 (F1 x NOD)BC1
NG00316-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00920-ICI 14.9 ICI T1D <17 (F1 x NOD)BC1
NG00525-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00507-PBS 18.7 PBS Spont. T1D (F1 x NOD)BC1
NG00524-ICI 14.1 ICI T1D <17 (F1 x NOD)BC1
NG00242-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00327-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00922-ICI 11.7 ICI T1D <17 (F1 x NOD)BC1
NG00527-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00538-PBS 21.3 PBS Spont. T1D (F1 x NOD)BC1
NG00551-ICI 13.1 ICI T1D <17 (F1 x NOD)BC1
NG00218-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00351-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00985-ICI 11.7 ICI T1D <17 (F1 x NOD)BC1
NG00565-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00548-PBS 25.4 PBS Spont. T1D (F1 x NOD)BC1
NG00577-ICI 14.6 ICI T1D <17 (F1 x NOD)BC1
NG00269-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00388-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG01021-ICI 11.1 ICI T1D <17 (F1 x NOD)BC1
NG00566-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00561-PBS 32.7 PBS Spont. T1D (F1 x NOD)BC1
NG00630-ICI 14.0 ICI T1D <17 (F1 x NOD)BC1
NG00293-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00389-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG01022-ICI 12.0 ICI T1D <17 (F1 x NOD)BC1
NG00567-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00586-PBS 16.6 PBS Spont. T1D (F1 x NOD)BC1
NG00662-ICI 12.7 ICI T1D <17 (F1 x NOD)BC1
NG00294-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00396-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00971-ICI 13.4 ICI T1D <17 (F1 x NOD)BC1
NG00647-PBS 35.1 PBS Spont. T1D (F1 x NOD)BC1
NG00606-PBS 35.0 PBS Spont. T1D (F1 x NOD)BC1
NG00698-ICI 14.9 ICI T1D <17 (F1 x NOD)BC1
NG00296-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00450-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00959-ICI 14.6 ICI T1D <17 (F1 x NOD)BC1
NG00673-PBS 39.0 PBS Spont. T1D (F1 x NOD)BC1
NG00638-PBS 20.3 PBS Spont. T1D (F1 x NOD)BC1
NG00777-ICI 14.1 ICI T1D <17 (F1 x NOD)BC1
NG00302-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00451-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00497-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00674-PBS 38.1 PBS Spont. T1D (F1 x NOD)BC1

All Problematic Samples

Neogen_Sample_ID no_pheno low_call.rate different_sex
NG00205-PBS XX
NG00686-PBS XX XX
NG00779-ICI XX
NG00305-EOI XX
NG01034-ICI XX
NG00499-EOI XX
NG00246-PBS XX
NG00241-ICI XX
NG00796-ICI XX
NG00309-EOI XX
NG00872-ICI XX
NG00530-EOI XX
NG00290-PBS XX
NG00249-ICI XX
NG00797-ICI XX
NG00310-EOI XX
NG00974-ICI XX
NG00532-EOI XX
NG00298-PBS XX
NG00494-ICI XX
NG00877-ICI XX
NG00324-EOI XX
NG00923-ICI XX
NG00534-EOI XX
NG00432-PBS XX
NG00498-ICI XX
NG00852-ICI XX
NG00316-EOI XX
NG00920-ICI XX
NG00525-EOI XX
NG00507-PBS XX
NG00524-ICI XX
NG00242-EOI XX
NG00327-EOI XX
NG00922-ICI XX
NG00527-EOI XX
NG00538-PBS XX
NG00551-ICI XX
NG00218-EOI XX
NG00351-EOI XX
NG00985-ICI XX
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R version 3.6.2 (2019-12-12)
Platform: x86_64-apple-darwin15.6.0 (64-bit)
Running under: macOS Catalina 10.15.7

Matrix products: default
BLAS:   /Library/Frameworks/R.framework/Versions/3.6/Resources/lib/libRblas.0.dylib
LAPACK: /Library/Frameworks/R.framework/Versions/3.6/Resources/lib/libRlapack.dylib

locale:
[1] en_AU.UTF-8/en_AU.UTF-8/en_AU.UTF-8/C/en_AU.UTF-8/en_AU.UTF-8

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
 [1] tibble_3.1.2      readxl_1.3.1      cluster_2.1.0     dplyr_0.8.5      
 [5] optparse_1.6.6    rhdf5_2.28.1      mclust_5.4.6      tidyr_1.0.2      
 [9] data.table_1.14.0 knitr_1.33        kableExtra_1.1.0  workflowr_1.6.2  

loaded via a namespace (and not attached):
 [1] tidyselect_1.0.0  xfun_0.24         purrr_0.3.4       colorspace_2.0-2 
 [5] vctrs_0.3.8       htmltools_0.5.1.1 getopt_1.20.3     viridisLite_0.4.0
 [9] yaml_2.2.1        utf8_1.2.1        rlang_0.4.11      later_1.0.0      
[13] pillar_1.6.1      glue_1.4.2        lifecycle_1.0.0   stringr_1.4.0    
[17] cellranger_1.1.0  munsell_0.5.0     rvest_0.3.5       evaluate_0.14    
[21] httpuv_1.5.2      fansi_0.5.0       Rcpp_1.0.7        readr_1.3.1      
[25] promises_1.1.0    scales_1.1.1      backports_1.2.1   webshot_0.5.2    
[29] fs_1.4.1          hms_0.5.3         digest_0.6.27     stringi_1.7.2    
[33] rprojroot_1.3-2   tools_3.6.2       magrittr_2.0.1    crayon_1.4.1     
[37] whisker_0.4       pkgconfig_2.0.3   ellipsis_0.3.2    xml2_1.3.1       
[41] assertthat_0.2.1  rmarkdown_2.1     httr_1.4.1        rstudioapi_0.13  
[45] Rhdf5lib_1.6.3    R6_2.5.0          git2r_0.26.1      compiler_3.6.2