Last updated: 2022-02-10

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Knit directory: Serreze-T1D_Workflow/

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Rmd d199bd4 Belinda Cornes 2022-02-10 QC analysis

Genotype Summary by Sample

Neogen_Sample_ID Missing AA AC AG AT CC CG GC GG TA TC TG TT Total_Genotyped Percent_Genotyped MURGIGV01_SNP.Count cr
NG00781-EOI 5211 28283 2269 9325 8 29223 7 3 29503 2 9028 2195 28202 138048 96.36% 143259 0.9636253219693
NG00878-EOI 5351 29583 1809 7299 7 30373 9 7 30639 2 7090 1722 29368 137908 96.26% 143259 0.962648070976344
NG00783-EOI 5283 28989 1941 8061 12 30043 8 5 30305 4 7716 1862 29030 137976 96.31% 143259 0.963122735744351
NG01225-ICI-LATE 5331 29709 1729 6974 3 30599 5 3 30908 1 6823 1667 29507 137928 96.28% 143259 0.962787678261052
NG00795-EOI 5251 29934 1593 6660 8 30858 5 5 31147 3 6421 1591 29783 138008 96.33% 143259 0.963346107399884
NG01303-ICI-LATE 5240 29316 1892 7710 10 30110 7 6 30379 2 7673 1886 29028 138019 96.34% 143259 0.963422891406474
NG00798-EOI 5223 28628 2070 8708 8 29570 7 5 29859 3 8549 2085 28544 138036 96.35% 143259 0.963541557598475
NG01483-ICI 5300 29492 1771 7284 8 30417 2 1 30720 2 7260 1749 29253 137959 96.30% 143259 0.963004069552349
NG00792-EOI 5248 29310 1863 7739 7 30149 10 3 30403 1 7686 1827 29013 138011 96.34% 143259 0.96336704849259
NG01464-ICI 5247 28767 2102 8606 9 29639 4 5 29945 4 8326 1947 28658 138012 96.34% 143259 0.963374028856826
NG00791-EOI 5311 29254 1931 7794 11 30074 6 4 30301 3 7567 1867 29136 137948 96.29% 143259 0.96292728554576
NG01465-ICI 58129 13888 4243 16140 22 8444 12 15 8608 16 16022 4093 13627 85130 59.42% 143259 0.594238407360096
NG00793-EOI 5391 30016 1582 6471 6 30878 8 3 31209 4 6225 1547 29919 137868 96.24% 143259 0.962368856406927
NG01493-ICI 5387 30059 1602 6387 13 30897 6 2 31229 4 6318 1567 29788 137872 96.24% 143259 0.962396777863869
NG00794-EOI 5321 29875 1637 6728 9 30851 11 2 31048 1 6430 1587 29759 137938 96.29% 143259 0.962857481903406
NG01495-ICI 53811 13239 4751 18539 21 8325 17 20 8560 24 18245 4650 13057 89448 62.44% 143259 0.624379620128578
NG00826-EOI 44442 24076 4091 15493 20 5937 10 8 6043 17 15234 3945 23943 98817 68.98% 143259 0.689778652650095
NG01506-ICI 5286 28796 2045 8402 11 29776 7 5 30065 2 8106 1958 28800 137973 96.31% 143259 0.963101794651645
NG00833-EOI 5311 29619 1666 7097 11 30634 9 5 30866 3 6836 1683 29519 137948 96.29% 143259 0.96292728554576
NG01507-ICI 5302 30170 1496 6134 5 31070 1 0 31448 1 6140 1493 29999 137957 96.30% 143259 0.962990108823878
NG00835-EOI 5374 30756 1248 5355 9 31592 2 2 31890 3 5248 1258 30522 137885 96.25% 143259 0.962487522598929
NG01513-ICI 5385 31126 1147 4745 10 31962 6 2 32234 3 4679 1125 30835 137874 96.24% 143259 0.96241073859234
NG00836-EOI 5371 28712 2107 8575 9 29523 9 6 29888 5 8396 2026 28632 137888 96.25% 143259 0.962508463691635
NG01518-ICI 46640 22326 4416 16983 22 4756 12 15 4807 14 16606 4313 22349 96619 67.44% 143259 0.674435812060673

Gender Summary by Sample

Neogen_Sample_ID Provided.Sex Inferred.Sex
NG00781-EOI F M
NG00878-EOI F M
NG00783-EOI F M
NG01225-ICI-LATE F M
NG00795-EOI F M
NG01303-ICI-LATE F M
NG00798-EOI F M
NG01483-ICI F M
NG00792-EOI F M
NG01464-ICI F M
NG00791-EOI F M
NG01465-ICI F F
NG00793-EOI F M
NG01493-ICI F M
NG00794-EOI F M
NG01495-ICI F F
NG00826-EOI F F
NG01506-ICI F M
NG00833-EOI F M
NG01507-ICI F M
NG00835-EOI F M
NG01513-ICI F M
NG00836-EOI F M
NG01518-ICI F F

Phenotype Summary by Sample

Neogen_Sample_ID age.of.onset group diabetic.status strain
NG00781-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00878-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG00783-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG01225-ICI-LATE 17.6 ICI T1D>17 (F1 x NOD)BC1
NG00795-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG01303-ICI-LATE 17.9 ICI T1D>17 (F1 x NOD)BC1
NG00798-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG01483-ICI 13.9 ICI T1D<17 (F1 x NOD)BC1
NG00792-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG01464-ICI 12.6 ICI T1D<17 (F1 x NOD)BC1
NG00791-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG01465-ICI 12.6 ICI T1D<17 (F1 x NOD)BC1
NG00793-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG01493-ICI 13.9 ICI T1D<17 (F1 x NOD)BC1
NG00794-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG01495-ICI 14.9 ICI T1D<17 (F1 x NOD)BC1
NG00826-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG01506-ICI 13.6 ICI T1D<17 (F1 x NOD)BC1
NG00833-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG01507-ICI 14.7 ICI T1D<17 (F1 x NOD)BC1
NG00835-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG01513-ICI 12.6 ICI T1D<17 (F1 x NOD)BC1
NG00836-EOI 0.0 EOI ICI-No Diabetes (F1 x NOD)BC1
NG01518-ICI 12.0 ICI T1D<17 (F1 x NOD)BC1

All Problematic Samples

Neogen_Sample_ID no_pheno low_call.rate different_sex
NG00781-EOI XX
NG00878-EOI XX
NG00783-EOI XX
NG01225-ICI-LATE XX
NG00795-EOI XX
NG01303-ICI-LATE XX
NG00798-EOI XX
NG01483-ICI XX
NG00792-EOI XX
NG01464-ICI XX
NG00791-EOI XX
NG01465-ICI XX
NG00793-EOI XX
NG01493-ICI XX
NG00794-EOI XX
NG01495-ICI XX
NG00826-EOI XX
NG01506-ICI XX
NG00833-EOI XX
NG01507-ICI XX
NG00835-EOI XX
NG01513-ICI XX
NG00836-EOI XX
NG01518-ICI XX

R version 3.6.2 (2019-12-12)
Platform: x86_64-apple-darwin15.6.0 (64-bit)
Running under: macOS Catalina 10.15.7

Matrix products: default
BLAS:   /Library/Frameworks/R.framework/Versions/3.6/Resources/lib/libRblas.0.dylib
LAPACK: /Library/Frameworks/R.framework/Versions/3.6/Resources/lib/libRlapack.dylib

locale:
[1] en_AU.UTF-8/en_AU.UTF-8/en_AU.UTF-8/C/en_AU.UTF-8/en_AU.UTF-8

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
 [1] tibble_3.1.2      readxl_1.3.1      cluster_2.1.0     dplyr_0.8.5      
 [5] optparse_1.6.6    rhdf5_2.28.1      mclust_5.4.6      tidyr_1.0.2      
 [9] data.table_1.14.0 knitr_1.33        kableExtra_1.1.0  workflowr_1.6.2  

loaded via a namespace (and not attached):
 [1] tidyselect_1.0.0  xfun_0.24         purrr_0.3.4       colorspace_2.0-2 
 [5] vctrs_0.3.8       htmltools_0.5.1.1 getopt_1.20.3     viridisLite_0.4.0
 [9] yaml_2.2.1        utf8_1.2.1        rlang_0.4.11      later_1.0.0      
[13] pillar_1.6.1      glue_1.4.2        lifecycle_1.0.0   stringr_1.4.0    
[17] cellranger_1.1.0  munsell_0.5.0     rvest_0.3.5       evaluate_0.14    
[21] httpuv_1.5.2      fansi_0.5.0       Rcpp_1.0.7        readr_1.3.1      
[25] promises_1.1.0    scales_1.1.1      backports_1.2.1   webshot_0.5.2    
[29] fs_1.4.1          hms_0.5.3         digest_0.6.27     stringi_1.7.2    
[33] rprojroot_1.3-2   tools_3.6.2       magrittr_2.0.1    crayon_1.4.1     
[37] whisker_0.4       pkgconfig_2.0.3   ellipsis_0.3.2    xml2_1.3.1       
[41] assertthat_0.2.1  rmarkdown_2.1     httr_1.4.1        rstudioapi_0.13  
[45] Rhdf5lib_1.6.3    R6_2.5.0          git2r_0.26.1      compiler_3.6.2