Quality control for BAL pool: BP1
Anson Wong
Molecular Immunity, Murdoch Children’s Research Institute2025-10-23
Last updated: 2025-10-23
Checks: 6 1
Knit directory: cf-eti-bal/
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knitr::opts_chunk$set(warning = FALSE, message = FALSE)
xaringanExtra::use_panelset()1 Load packages
suppressPackageStartupMessages({
library(qs)
library(DropletUtils)
library(here)
library(ggplot2)
library(Seurat)
library(cowplot)
library(patchwork)
library(scater)
library(dplyr)
library(forcats)
library(janitor)
library(stringr)
library(AnnotationHub)
library(ensembldb)
library(msigdbr)
library(Homo.sapiens)
})
set.seed(1990)2 Set names for this pool
# Specify batch name
batch_name <- "BP1"
num_of_captures <- 3
# Specify capture name
capture_names <- c(paste0(batch_name, "-c",1:num_of_captures))
capture_names <- setNames(capture_names, capture_names)
# Assign sample ID to HTO ID
# Manually list sample names matching HTO id (in alphabetical order)
samples <- c("M1C198B", #HTO3
"M1C208A", #HTO6
"M1C166(1)", #HTO7
"M1C199B", #HTO8
"M1C207B", #HTO10
"M1N087", #HTO12
"M1N080" #HTO13
)3 Filter out ambiguous droplets
# read demultiplexing outputs
sce <- readRDS(here("data",
"SCEs",
"demux",
paste0(batch_name,".cellbender.demux.SCE.rds")))
# number of droplets before removal
dim(sce)[1] 36601 62050# A. remove droplets classified as "doublets" by Vireo
sce <- sce[, which(sce$sampleID.genetics != "Doublet")]
# number of droplets after doublet removal
dim(sce)[1] 36601 55465# look for the following droplets
## genetically non-negatives with matched sampleID.genetics and sampleID.HTO
## or classified as Doublet or Negative by HTODemux
nneg_match <- sce$sampleID.genetics != "Negative" &
(sce$sampleID.HTO == sce$sampleID.genetics |
sce$sampleID.HTO %in% c("Doublet","Negative"))
## genetically negatives but with HTO classification
neg_match <- sce$sampleID.genetics == "Negative" &
sce$sampleID.HTO %in% samples
# B. keep droplets that match the demultiplexing criteria
sce <- sce[, nneg_match | neg_match]
# number of droplets after demultiplexing
dim(sce)[1] 36601 49781# C. remove droplets with posterior counts of zero called by CellBender
# see: https://github.com/broadinstitute/CellBender/issues/111
sce <- sce[, colSums(counts(sce)) != 0]
# number of droplets after removing droplets with zero counts
dim(sce)[1] 36601 49210# rename sampleID
sampleID <- setNames(factor(case_when(
sce$sampleID.genetics == "Negative" ~ sce$sampleID.HTO,
TRUE ~ sce$sampleID.genetics),levels = samples),
colnames(sce))
sce$sampleID <- sampleID4 Visualize results after filtering
# number of droplets assigned by HTO method
p1 <- ggcells(sce) +
geom_bar(aes(x = sampleID.HTO, fill = sampleID.HTO)) +
geom_text(stat='count', aes(x = sampleID.HTO, label=..count..), hjust=1, size=2) +
coord_flip() +
ggtitle("By HTO") +
ylab("Number of droplets") +
theme_cowplot(font_size = 8) +
scale_y_continuous(breaks=seq(0,12000,3000),limits=c(0,16000)) +
scale_fill_manual(values = sce$colours$sampleID.HTO_colours) +
theme(axis.title.y = element_blank()) +
guides(fill=guide_legend(title="sampleID"))
p1.facet <- ggcells(sce) +
geom_bar(aes(x = sampleID.HTO, fill = sampleID.HTO)) +
geom_text(stat='count', aes(x = sampleID.HTO, label=..count..),
hjust=1, size=2) +
#ggtitle("By HTO") +
ylab("Number of droplets") +
facet_grid(~Capture, scales = "fixed", space = "fixed") +
theme_cowplot(font_size = 8) +
scale_y_continuous(breaks=seq(0,6000,2000), limits = c(0,6000)) +
scale_fill_manual(values = sce$colours$sampleID.HTO_colours) +
theme(axis.title.y = element_blank()) +
guides(fill=guide_legend(title="sampleID")) +
coord_flip()
# number of droplets assigned by genetic method
p2 <- ggcells(sce) +
geom_bar(aes(x = sampleID.genetics, fill = sampleID.genetics)) +
geom_text(stat='count', aes(x = sampleID.genetics, label=..count..), hjust=1, size=2) +
coord_flip() +
ggtitle("By genetics") +
ylab("Number of droplets") +
theme_cowplot(font_size = 8) +
scale_y_continuous(breaks=seq(0,12000,3000), limits = c(0,16000)) +
scale_fill_manual(values = sce$colours$sampleID.genetics_colours) +
theme(axis.title.y = element_blank()) +
guides(fill=FALSE)
p2.facet <- ggcells(sce) +
geom_bar(aes(x = sampleID.genetics, fill = sampleID.genetics)) +
geom_text(stat='count', aes(x = sampleID.genetics, label=..count..),
hjust=1, size=2) +
ylab("Number of droplets") +
facet_grid(~Capture, scales = "fixed", space = "fixed") +
theme_cowplot(font_size = 8) +
scale_y_continuous(breaks=seq(0,6000,2000), limits = c(0,6000)) +
scale_fill_manual(values = sce$colours$sampleID.genetics_colours) +
theme(axis.title.y = element_blank()) +
guides(fill=guide_legend(title="sampleID")) +
coord_flip()
(p1+p1.facet+plot_layout(width=c(1,2))) /
(p2+p2.facet+plot_layout(width=c(1,2))) +
plot_layout(guides="collect")
# proportion of genetically assigned droplets in each HTO
p3 <- ggcells(sce) +
geom_bar(
aes(x = sampleID.HTO, fill = sampleID.genetics),
position = position_fill(reverse = TRUE)) +
coord_flip() +
ylab("Frequency") +
theme_cowplot(font_size = 8) +
scale_fill_manual(values = sce$colours$sampleID.genetics_colours)
# proportion of HTO assigned droplets in each genetic donor
p4 <- ggcells(sce) +
geom_bar(
aes(x = sampleID.genetics, fill = sampleID.HTO),
position = position_fill(reverse = TRUE)) +
coord_flip() +
ylab("Frequency") +
theme_cowplot(font_size = 8) +
scale_fill_manual(values = sce$colours$sampleID.HTO_colours)
((p3 + ggtitle("By HTO")) +
p3 + facet_grid(~Capture) + plot_layout(widths = c(1, 2))) /
((p4 + ggtitle("By genetics")) +
p4 + facet_grid(~Capture) + plot_layout(widths = c(1, 2))) +
plot_layout(guides = "collect")
5 Add metadata and sex check
# read metadata.csv
metadata <- read.csv(here("data",
"sample_sheets",
paste0(batch_name,".metadata.csv")))
i <- match(sce$sampleID, metadata$sampleID)
# add patient demographics
colData(sce) <- cbind(
colData(sce),
metadata[i,c("Age","Sex","Condition","Bronchiectasis")]
)
# sex check by the expression of XIST, a female-specific gene.
# this detects certain types of sample-mix-ups.
plotExpression(
sce,
"XIST",
x = "sampleID",
colour_by = "Sex",
exprs_values = "counts",
swap_rownames = "Symbol") +
scale_colour_manual(
values = c(
"Female" = "deeppink3",
"Male" = "deepskyblue"),
name = "Sex") +
theme_cowplot() +
theme(axis.text.x = element_text(angle = 45, vjust = 1, hjust = 1))
6 Add feature-based annotation
# make feature names unique
rownames(sce) <- uniquifyFeatureNames(rowData(sce)$ID, rowData(sce)$Symbol)
# prepare ensembl v98 database
ah <- AnnotationHub(cache="/group/canc2/anson/.cache/R/AnnotationHub", ask=FALSE)
EnsDb.Hsapiens.v98 <- query(ah, c("EnsDb", "Homo Sapiens", 98))[[1]]
ensdb_columns <- setNames(c("GENEBIOTYPE", "SEQNAME"),
paste0("ENSEMBL.",
c("GENEBIOTYPE", "SEQNAME")))
stopifnot(all(ensdb_columns %in% columns(EnsDb.Hsapiens.v98)))
ensdb_df <- DataFrame(
lapply(ensdb_columns, function(column) {
mapIds(
x = EnsDb.Hsapiens.v98,
keys = rowData(sce)$ID,
keytype = "GENEID",
column = column,
multiVals = "first")
}),
row.names = rowData(sce)$ID)
# prepare ncbi database
ncbi_columns <- setNames(c("ALIAS", "ENTREZID", "GENENAME"),
paste0("NCBI.", c("ALIAS", "ENTREZID", "GENENAME")))
stopifnot(all(ncbi_columns %in% columns(Homo.sapiens)))
ncbi_df <- DataFrame(
lapply(ncbi_columns, function(column) {
mapIds(
x = Homo.sapiens,
keys = rowData(sce)$ID,
keytype = "ENSEMBL",
column = column,
multiVals = "CharacterList")
}),
row.names = rowData(sce)$ID)
rowData(sce) <- cbind(rowData(sce), ensdb_df, ncbi_df)7 QC
Some code in this section are derived from Dr. Jovana Maksimovic’s work for Maksimovic et al. 2022
7.1 Calculate metrics
# prepare gene sets
## mitochondrial gene set
mito_set <- rownames(sce)[which(rowData(sce)$ENSEMBL.SEQNAME == "MT")]
is_mito <- rownames(sce) %in% mito_set
summary(is_mito) Mode FALSE TRUE
logical 36588 13 ## ribosomal gene set
ribo_set <- grep("^RP(S|L)", rownames(sce), value = TRUE)
c2_sets <- msigdbr(species = "Homo sapiens", category = "C2")
ribo_set <- union(
ribo_set,
c2_sets[c2_sets$gs_name == "KEGG_RIBOSOME", ]$human_gene_symbol)
is_ribo <- rownames(sce) %in% ribo_set
summary(is_ribo) Mode FALSE TRUE
logical 36494 107 # calculate QC metrics
sce <- addPerCellQCMetrics(
sce,
subsets = list(Mito = which(is_mito),
Ribo = which(is_ribo)),
use.altexps = NULL)7.2 QC: nCount and nFeature
7.2.1 Visualize metrics before removal
# library size
p1 <- plotColData(
sce,
"sum",
x = "sampleID",
colour_by = "sampleID",
point_size = 0.5) +
scale_y_log10() +
scale_colour_manual(values = sce$colours$sample_colours, name = "sampleID") +
theme(axis.text.x = element_blank())
# number of genes detected
p2 <- plotColData(
sce,
"detected",
x = "sampleID",
colour_by = "sampleID",
point_size = 0.5) +
scale_colour_manual(values = sce$colours$sample_colours, name = "sampleID") +
theme(axis.text.x = element_blank())
((p1 + NoLegend()) + p1 + facet_grid(~sce$Capture) + plot_layout(widths=c(1, length(capture_names))))/
((p2 + NoLegend()) + p2 + facet_grid(~sce$Capture) + plot_layout(widths=c(1, length(capture_names)))) +
plot_layout(guides="collect")
7.2.2 Remove cells with features < 200
sce$batch <- interaction(
sce$Capture,
sce$sampleID,
drop = TRUE,
lex.order = FALSE)
feature_drop <- sce$detected < 200
sce_pre_QC_outlier_removal <- sce
keep <- !feature_drop
sce_pre_QC_outlier_removal$keep <- keep
sce <- sce[, keep]
data.frame(
ByFeature = tapply(
feature_drop,
sce_pre_QC_outlier_removal$batch,
sum,
na.rm = TRUE),
Remaining = as.vector(unname(table(sce$batch))),
PercRemaining = round(
100 * as.vector(unname(table(sce$batch))) /
as.vector(
unname(
table(sce_pre_QC_outlier_removal$batch))), 1)) |>
tibble::rownames_to_column("batch") |>
dplyr::arrange(dplyr::desc(PercRemaining)) |>
DT::datatable(
caption = "Number of droplets removed by each QC step and the number of droplets remaining.",
rownames = FALSE) |>
DT::formatRound("PercRemaining", 1)7.2.3 Visualize metrics after removal
p3 <- plotColData(
sce_pre_QC_outlier_removal,
"sum",
x = "sampleID",
colour_by = "keep",
point_size = 0.5) +
theme(axis.text.x = element_text(angle = 45, hjust = 1))
p4 <- plotColData(
sce_pre_QC_outlier_removal,
"detected",
x = "sampleID",
colour_by = "keep",
point_size = 0.5) +
theme(axis.text.x = element_text(angle = 45, hjust = 1))
((p3 + NoLegend()) + p3 + facet_grid(~sce_pre_QC_outlier_removal$Capture) + plot_layout(widths=c(1, length(capture_names))))/
((p4 + NoLegend()) + p4 + facet_grid(~sce_pre_QC_outlier_removal$Capture) + plot_layout(widths=c(1, length(capture_names)))) +
plot_layout(guides="collect")
7.3 QC: mitochondrial percent
7.3.1 Visualize metrics before removal
# subsets_Mito_percent
p1 <- plotColData(
sce,
"subsets_Mito_percent",
x = "sampleID",
colour_by = "sampleID",
point_size = 0.5) +
scale_y_log10() +
scale_colour_manual(values = sce$colours$sample_colours, name = "sampleID") +
theme(axis.text.x = element_blank())
# subsets_Ribo_percent
p2 <- plotColData(
sce,
"subsets_Ribo_percent",
x = "sampleID",
colour_by = "sampleID",
point_size = 0.5) +
scale_colour_manual(values = sce$colours$sample_colours, name = "sampleID") +
theme(axis.text.x = element_blank())
((p1 + NoLegend()) + p1 + facet_grid(~sce$Capture) + plot_layout(widths=c(1, length(capture_names))))/
((p2 + NoLegend()) + p2 + facet_grid(~sce$Capture) + plot_layout(widths=c(1, length(capture_names)))) +
plot_layout(guides="collect")
7.3.2 Remove mito% outlier
mito_drop <- isOutlier(
metric = sce$subsets_Mito_percent,
nmads = 3,
type = "higher",
batch = sce$batch)
sce_pre_QC_outlier_removal <- sce
keep <- !mito_drop
sce_pre_QC_outlier_removal$keep <- keep
sce <- sce[, keep]
data.frame(
ByMito = tapply(
mito_drop,
sce_pre_QC_outlier_removal$batch,
sum,
na.rm = TRUE),
Remaining = as.vector(unname(table(sce$batch))),
PercRemaining = round(
100 * as.vector(unname(table(sce$batch))) /
as.vector(
unname(
table(sce_pre_QC_outlier_removal$batch))), 1)) |>
tibble::rownames_to_column("batch") |>
dplyr::arrange(dplyr::desc(PercRemaining)) |>
DT::datatable(
caption = "Number of droplets removed by each QC step and the number of droplets remaining.",
rownames = FALSE) |>
DT::formatRound("PercRemaining", 1)7.3.1 Visualize metrics after removal
p3 <- plotColData(
sce_pre_QC_outlier_removal,
"subsets_Mito_percent",
x = "sampleID",
colour_by = "keep",
point_size = 0.5) +
theme(axis.text.x = element_text(angle = 45, hjust = 1))
p4 <- plotColData(
sce_pre_QC_outlier_removal,
"subsets_Ribo_percent",
x = "sampleID",
colour_by = "keep",
point_size = 0.5) +
theme(axis.text.x = element_text(angle = 45, hjust = 1))
((p3 + NoLegend()) + p3 + facet_grid(~sce_pre_QC_outlier_removal$Capture) + plot_layout(widths=c(1, length(capture_names))))/
((p4 + NoLegend()) + p4 + facet_grid(~sce_pre_QC_outlier_removal$Capture) + plot_layout(widths=c(1, length(capture_names)))) +
plot_layout(guides="collect")
7.5.2 Number of droplets retained (named by sample ID)
# number of droplets assigned by HTO method
p1 <- ggcells(sce) +
geom_bar(aes(x = sampleID.HTO, fill = sampleID.HTO)) +
geom_text(stat='count', aes(x = sampleID.HTO, label=..count..), hjust=1, size=2) +
coord_flip() +
ggtitle("By HTO") +
ylab("Number of droplets") +
theme_cowplot(font_size = 8) +
scale_y_continuous(breaks=seq(0,16000,4000),limits=c(0,16000)) +
scale_fill_manual(values = sce$colours$sampleID.HTO_colours) +
theme(axis.title.y = element_blank()) +
guides(fill=guide_legend(title="sampleID"))
p1.facet <- ggcells(sce) +
geom_bar(aes(x = sampleID.HTO, fill = sampleID.HTO)) +
geom_text(stat='count', aes(x = sampleID.HTO, label=..count..),
hjust=1, size=2) +
#ggtitle("By HTO") +
ylab("Number of droplets") +
facet_grid(~Capture, scales = "fixed", space = "fixed") +
theme_cowplot(font_size = 8) +
scale_y_continuous(breaks=seq(0,4000,1000), limits = c(0,4000)) +
scale_fill_manual(values = sce$colours$sampleID.HTO_colours) +
theme(axis.title.y = element_blank()) +
guides(fill=guide_legend(title="sampleID")) +
coord_flip()
# number of droplets assigned by genetic method
p2 <- ggcells(sce) +
geom_bar(aes(x = sampleID.genetics, fill = sampleID.genetics)) +
geom_text(stat='count', aes(x = sampleID.genetics, label=..count..), hjust=1, size=2) +
coord_flip() +
ggtitle("By genetics") +
ylab("Number of droplets") +
theme_cowplot(font_size = 8) +
scale_y_continuous(breaks=seq(0,16000,4000), limits = c(0,16000)) +
scale_fill_manual(values = sce$colours$sampleID.genetics_colours) +
theme(axis.title.y = element_blank()) +
guides(fill=FALSE)
p2.facet <- ggcells(sce) +
geom_bar(aes(x = sampleID.genetics, fill = sampleID.genetics)) +
geom_text(stat='count', aes(x = sampleID.genetics, label=..count..),
hjust=1, size=2) +
ylab("Number of droplets") +
facet_grid(~Capture, scales = "fixed", space = "fixed") +
theme_cowplot(font_size = 8) +
scale_y_continuous(breaks=seq(0,4000,1000), limits = c(0,4000)) +
scale_fill_manual(values = sce$colours$sampleID.genetics_colours) +
theme(axis.title.y = element_blank()) +
guides(fill=guide_legend(title="sampleID")) +
coord_flip()
(p1+p1.facet+plot_layout(width=c(1,2))) /
(p2+p2.facet+plot_layout(width=c(1,2))) +
plot_layout(guides="collect")
7.5.3 Proportion of droplets (named by sample ID)
# proportion of genetically assigned droplets in each HTO
p3 <- ggcells(sce) +
geom_bar(
aes(x = sampleID.HTO, fill = sampleID.genetics),
position = position_fill(reverse = TRUE)) +
coord_flip() +
ylab("Frequency") +
theme_cowplot(font_size = 8) +
scale_fill_manual(values = sce$colours$sampleID.genetics_colours)
# proportion of HTO assigned droplets in each genetic donor
p4 <- ggcells(sce) +
geom_bar(
aes(x = sampleID.genetics, fill = sampleID.HTO),
position = position_fill(reverse = TRUE)) +
coord_flip() +
ylab("Frequency") +
theme_cowplot(font_size = 8) +
scale_fill_manual(values = sce$colours$sampleID.HTO_colours)
((p3 + ggtitle("By HTO")) +
p3 + facet_grid(~Capture) + plot_layout(widths = c(1, 2))) /
((p4 + ggtitle("By genetics")) +
p4 + facet_grid(~Capture) + plot_layout(widths = c(1, 2))) +
plot_layout(guides = "collect")
8 Remove undesired genes
dim(sce)[1] 36601 40083# remove low-abundance genes
numCells <- nexprs(sce, byrow = TRUE)
keep <- numCells > 20
sum(keep)[1] 24731sce <- sce[keep,]
# number of genes retained
dim(sce)[1] 24731 400839 Save object
prep_dir <- here("data","SCEs","preprocessed")
if(!dir.exists(prep_dir)) {
dir.create(prep_dir, recursive = TRUE)
}
out <- paste0(prep_dir,'/',
paste0(batch_name,".preprocessed.SCE.qs"))
if(!file.exists(out)) qsave(sce, out, nthreads = 16)References: Maksimovic J, Shanthikumar S, Howitt G, Hickey PF, Ho W, Anttila C, et al. Single-cell atlas of bronchoalveolar lavage from preschool cystic fibrosis reveals new cell phenotypes. bioRxiv 2022: 2022.2006.2017.496207.
sessionInfo()R version 4.1.2 (2021-11-01)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: CentOS Linux 7 (Core)
Matrix products: default
BLAS: /hpc/software/installed/R/4.1.2/lib64/R/lib/libRblas.so
LAPACK: /hpc/software/installed/R/4.1.2/lib64/R/lib/libRlapack.so
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
[3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8
[5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8
[7] LC_PAPER=en_US.UTF-8 LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
attached base packages:
[1] stats4 stats graphics grDevices utils datasets methods
[8] base
other attached packages:
[1] Homo.sapiens_1.3.1
[2] TxDb.Hsapiens.UCSC.hg19.knownGene_3.2.2
[3] org.Hs.eg.db_3.14.0
[4] GO.db_3.14.0
[5] OrganismDbi_1.36.0
[6] msigdbr_7.5.1
[7] ensembldb_2.18.4
[8] AnnotationFilter_1.22.0
[9] GenomicFeatures_1.46.4
[10] AnnotationDbi_1.56.2
[11] AnnotationHub_3.2.2
[12] BiocFileCache_2.6.1
[13] dbplyr_2.1.1
[14] stringr_1.5.1
[15] janitor_2.1.0
[16] forcats_0.5.1
[17] dplyr_1.1.4
[18] scater_1.22.0
[19] scuttle_1.4.0
[20] patchwork_1.2.0
[21] cowplot_1.1.3
[22] SeuratObject_5.0.1
[23] Seurat_4.4.0
[24] ggplot2_3.5.2
[25] here_1.0.1
[26] DropletUtils_1.14.2
[27] SingleCellExperiment_1.16.0
[28] SummarizedExperiment_1.24.0
[29] Biobase_2.54.0
[30] GenomicRanges_1.46.1
[31] GenomeInfoDb_1.30.1
[32] IRanges_2.28.0
[33] S4Vectors_0.32.4
[34] BiocGenerics_0.40.0
[35] MatrixGenerics_1.6.0
[36] matrixStats_1.1.0
[37] qs_0.27.2
loaded via a namespace (and not attached):
[1] rappdirs_0.3.3 rtracklayer_1.54.0
[3] scattermore_1.2 R.methodsS3_1.8.1
[5] tidyr_1.3.1 bit64_4.6.0-1
[7] knitr_1.46 irlba_2.3.5.1
[9] DelayedArray_0.20.0 R.utils_2.12.2
[11] data.table_1.15.4 KEGGREST_1.34.0
[13] RCurl_1.98-1.9 generics_0.1.3
[15] ScaledMatrix_1.2.0 RSQLite_2.3.6
[17] RApiSerialize_0.1.4 RANN_2.6.1
[19] future_1.33.2 bit_4.0.5
[21] xml2_1.3.3 spatstat.data_3.0-4
[23] lubridate_1.8.0 httpuv_1.6.15
[25] assertthat_0.2.1 viridis_0.6.2
[27] xfun_0.43 hms_1.1.1
[29] jquerylib_0.1.4 babelgene_22.9
[31] evaluate_0.23 promises_1.3.0
[33] restfulr_0.0.15 fansi_1.0.6
[35] progress_1.2.2 igraph_2.0.3
[37] DBI_1.2.2 htmlwidgets_1.6.4
[39] spatstat.geom_3.2-9 purrr_1.0.1
[41] ellipsis_0.3.2 crosstalk_1.2.1
[43] RcppParallel_5.1.10 biomaRt_2.50.3
[45] deldir_2.0-4 sparseMatrixStats_1.6.0
[47] vctrs_0.6.5 ROCR_1.0-11
[49] abind_1.4-8 cachem_1.0.8
[51] withr_3.0.0 progressr_0.14.0
[53] sctransform_0.4.1 GenomicAlignments_1.30.0
[55] prettyunits_1.1.1 xaringanExtra_0.7.0
[57] goftest_1.2-3 cluster_2.1.8.1
[59] dotCall64_1.1-1 lazyeval_0.2.2
[61] crayon_1.5.2 spatstat.explore_3.2-7
[63] labeling_0.4.3 edgeR_4.4.2
[65] pkgconfig_2.0.3 ProtGenerics_1.30.0
[67] nlme_3.1-155 vipor_0.4.5
[69] rlang_1.1.3 globals_0.16.3
[71] lifecycle_1.0.4 miniUI_0.1.1.1
[73] filelock_1.0.3 rsvd_1.0.5
[75] rprojroot_2.1.1 polyclip_1.10-6
[77] lmtest_0.9-40 graph_1.72.0
[79] Matrix_1.6-5 Rhdf5lib_1.16.0
[81] zoo_1.8-12 beeswarm_0.4.0
[83] ggridges_0.5.6 rjson_0.2.21
[85] png_0.1-8 viridisLite_0.4.2
[87] stringfish_0.16.0 bitops_1.0-9
[89] R.oo_1.24.0 KernSmooth_2.23-20
[91] spam_2.10-0 rhdf5filters_1.6.0
[93] Biostrings_2.62.0 blob_1.2.4
[95] DelayedMatrixStats_1.16.0 workflowr_1.7.0
[97] parallelly_1.37.1 spatstat.random_3.2-3
[99] beachmat_2.10.0 scales_1.3.0
[101] memoise_2.0.1 magrittr_2.0.3
[103] plyr_1.8.9 ica_1.0-3
[105] zlibbioc_1.48.2 compiler_4.1.2
[107] BiocIO_1.8.0 dqrng_0.3.2
[109] RColorBrewer_1.1-3 fitdistrplus_1.1-11
[111] Rsamtools_2.10.0 snakecase_0.11.0
[113] cli_3.6.2 XVector_0.34.0
[115] listenv_0.9.1 pbapply_1.7-2
[117] MASS_7.3-55 tidyselect_1.2.1
[119] stringi_1.8.3 highr_0.10
[121] yaml_2.3.8 BiocSingular_1.10.0
[123] locfit_1.5-9.4 ggrepel_0.9.1
[125] grid_4.1.2 sass_0.4.9
[127] tools_4.1.2 future.apply_1.11.2
[129] parallel_4.1.2 rstudioapi_0.13
[131] git2r_0.31.0 gridExtra_2.3
[133] farver_2.1.1 Rtsne_0.17
[135] digest_0.6.35 BiocManager_1.30.25
[137] shiny_1.8.1.1 Rcpp_1.0.12
[139] BiocVersion_3.18.1 later_1.3.2
[141] RcppAnnoy_0.0.22 httr_1.4.7
[143] colorspace_2.1-0 XML_3.99-0.16.1
[145] fs_1.6.4 tensor_1.5.1
[147] reticulate_1.36.1.9000 splines_4.1.2
[149] RBGL_1.70.0 uwot_0.1.14
[151] statmod_1.5.0 spatstat.utils_3.1-3
[153] sp_2.1-3 plotly_4.10.4.9000
[155] xtable_1.8-4 jsonlite_1.8.8
[157] R6_2.5.1 pillar_1.9.0
[159] htmltools_0.5.8.1 mime_0.12
[161] DT_0.20 glue_1.7.0
[163] fastmap_1.1.1 BiocParallel_1.28.3
[165] BiocNeighbors_1.12.0 interactiveDisplayBase_1.32.0
[167] codetools_0.2-20 utf8_1.2.4
[169] lattice_0.22-7 bslib_0.3.1
[171] spatstat.sparse_3.0-3 tibble_3.2.1
[173] curl_5.2.1 ggbeeswarm_0.6.0
[175] leiden_0.4.3.1 survival_3.2-13
[177] limma_3.62.2 rmarkdown_2.26
[179] munsell_0.5.1 rhdf5_2.38.1
[181] GenomeInfoDbData_1.2.11 HDF5Array_1.22.1
[183] reshape2_1.4.4 gtable_0.3.5