Last updated: 2017-11-29

Code version: 3d5d193

Setup

library("dplyr")
Warning: Installed Rcpp (0.12.13) different from Rcpp used to build dplyr (0.12.10).
Please reinstall dplyr to avoid random crashes or undefined behavior.
library("DT")
library("ggplot2")
library("reshape2")
library("Biobase")
theme_set(cowplot::theme_cowplot())
fname <- Sys.glob("../data/eset/*.rds")
eset <- Reduce(combine, Map(readRDS, fname))
pData(eset)$experiment <- as.factor(pData(eset)$experiment)

Total sequencing depth

ggplot(pData(eset), aes(x = raw, color = experiment)) +
  geom_density() +
  labs(x = "Number of raw sequences per single cell", y = "Number of cells",
       title = "Distribution of total raw sequences per single cell") +
  scale_color_discrete(name = "C1 chip")
Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Sum of sequences across the 96 single cells per C1 chip.

total_per_experiment <- pData(eset) %>%
  group_by(experiment) %>%
  summarize(raw = sum(raw),
            mapped = sum(mapped),
            molecules = sum(molecules))
datatable(total_per_experiment,
          options = list(pageLength = nrow(total_per_experiment)),
          colnames = c("C1 chip", "Number of raw sequences",
                       "Number of mapped",
                       "Number of molecules"))
ggplot(melt(total_per_experiment, id.vars = "experiment",
            variable.name = "type", value.name = "count"),
       aes(x = experiment, y = count, color = type)) +
  geom_point() +
  labs(title = "Sequencing depth per C1 chip",
       x = "C1 chip", y = "Number of sequences") +
  theme(legend.title = element_blank(),
        axis.text.x = element_text(angle = 45, hjust = 1, vjust = 1))
Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Warning in structure(NULL, class = "waiver"): Calling 'structure(NULL, *)' is deprecated, as NULL cannot have attributes.
  Consider 'structure(list(), *)' instead.

Session information

sessionInfo()
R version 3.4.1 (2017-06-30)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Scientific Linux 7.2 (Nitrogen)

Matrix products: default
BLAS: /home/jdblischak/software/miniconda3/envs/fucci-seq/lib/R/lib/libRblas.so
LAPACK: /home/jdblischak/software/miniconda3/envs/fucci-seq/lib/R/lib/libRlapack.so

locale:
 [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C              
 [3] LC_TIME=en_US.UTF-8        LC_COLLATE=en_US.UTF-8    
 [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
 [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                 
 [9] LC_ADDRESS=C               LC_TELEPHONE=C            
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       

attached base packages:
[1] parallel  stats     graphics  grDevices utils     datasets  methods  
[8] base     

other attached packages:
[1] Biobase_2.38.0      BiocGenerics_0.24.0 reshape2_1.4.2     
[4] ggplot2_2.2.0       DT_0.2              dplyr_0.7.0        

loaded via a namespace (and not attached):
 [1] Rcpp_0.12.13     knitr_1.16       magrittr_1.5     cowplot_0.8.0   
 [5] munsell_0.4.3    colorspace_1.3-2 R6_2.2.0         rlang_0.1.2     
 [9] plyr_1.8.4       stringr_1.2.0    tools_3.4.1      grid_3.4.1      
[13] gtable_0.2.0     git2r_0.19.0     htmltools_0.3.6  lazyeval_0.2.0  
[17] yaml_2.1.14      rprojroot_1.2    digest_0.6.12    assertthat_0.1  
[21] tibble_1.3.3     htmlwidgets_0.9  glue_1.1.1       evaluate_0.10.1 
[25] rmarkdown_1.6    labeling_0.3     stringi_1.1.2    compiler_3.4.1  
[29] scales_0.4.1     backports_1.0.5  jsonlite_1.4

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