Last updated: 2023-05-17

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Knit directory: Cardiotoxicity/

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##package loading

48 hour Lactate Dehydrogenase results

48 hour viability to LDH activity correlation

####24h LDH data frame

$VDA

    Welch Two Sample t-test

data:  VE_24_ldh and DA_24_ldh
t = -3.7541, df = 17.12, p-value = 0.001564
alternative hypothesis: true difference in means is not equal to 0
95 percent confidence interval:
 -1.0263033 -0.2880301
sample estimates:
mean of x mean of y 
 1.012944  1.670111 


$VDX

    Welch Two Sample t-test

data:  VE_24_ldh and DX_24_ldh
t = -3.7427, df = 17.065, p-value = 0.001611
alternative hypothesis: true difference in means is not equal to 0
95 percent confidence interval:
 -1.3902576 -0.3880757
sample estimates:
mean of x mean of y 
 1.012944  1.902111 


$VEP

    Welch Two Sample t-test

data:  VE_24_ldh and EP_24_ldh
t = -4.285, df = 17.065, p-value = 0.0004969
alternative hypothesis: true difference in means is not equal to 0
95 percent confidence interval:
 -1.5254695 -0.5190861
sample estimates:
mean of x mean of y 
 1.012944  2.035222 


$VMT

    Welch Two Sample t-test

data:  VE_24_ldh and MT_24_ldh
t = -5.1821, df = 17.085, p-value = 7.383e-05
alternative hypothesis: true difference in means is not equal to 0
95 percent confidence interval:
 -1.5220379 -0.6415176
sample estimates:
mean of x mean of y 
 1.012944  2.094722 


$VTR

    Welch Two Sample t-test

data:  VE_24_ldh and TR_24_ldh
t = -2.5982, df = 17.112, p-value = 0.01868
alternative hypothesis: true difference in means is not equal to 0
95 percent confidence interval:
 -0.85356981 -0.08876353
sample estimates:
mean of x mean of y 
 1.012944  1.484111 


$VVEH

    Welch Two Sample t-test

data:  VE_24_ldh and VE_24_ldh
t = 0, df = 34, p-value = 1
alternative hypothesis: true difference in means is not equal to 0
95 percent confidence interval:
 -0.02989169  0.02989169
sample estimates:
mean of x mean of y 
 1.012944  1.012944 

####24h Tnni data frame

$VDAT

    Welch Two Sample t-test

data:  VE_24_TNNI and DA_24_TNNI
t = -1.2565, df = 11.832, p-value = 0.2332
alternative hypothesis: true difference in means is not equal to 0
95 percent confidence interval:
 -0.36079840  0.09713173
sample estimates:
mean of x mean of y 
 0.978500  1.110333 


$VDXT

    Welch Two Sample t-test

data:  VE_24_TNNI and DX_24_TNNI
t = -5.8512, df = 15.728, p-value = 2.633e-05
alternative hypothesis: true difference in means is not equal to 0
95 percent confidence interval:
 -0.3799972 -0.1776694
sample estimates:
mean of x mean of y 
 0.978500  1.257333 


$VEPT

    Welch Two Sample t-test

data:  VE_24_TNNI and EP_24_TNNI
t = -3.4195, df = 13.915, p-value = 0.004181
alternative hypothesis: true difference in means is not equal to 0
95 percent confidence interval:
 -0.32673646 -0.07476354
sample estimates:
mean of x mean of y 
  0.97850   1.17925 


$VMTT

    Welch Two Sample t-test

data:  VE_24_TNNI and MT_24_TNNI
t = -4.4919, df = 12.316, p-value = 0.0006915
alternative hypothesis: true difference in means is not equal to 0
95 percent confidence interval:
 -0.5625613 -0.1957720
sample estimates:
mean of x mean of y 
 0.978500  1.357667 


$VTRT

    Welch Two Sample t-test

data:  VE_24_TNNI and TR_24_TNNI
t = -3.7217, df = 11.904, p-value = 0.002956
alternative hypothesis: true difference in means is not equal to 0
95 percent confidence interval:
 -0.5951287 -0.1553713
sample estimates:
mean of x mean of y 
  0.97850   1.35375 


$VVEHT

    Welch Two Sample t-test

data:  VE_24_TNNI and VE_24_TNNI
t = 0, df = 34, p-value = 1
alternative hypothesis: true difference in means is not equal to 0
95 percent confidence interval:
 -0.0574163  0.0574163
sample estimates:
mean of x mean of y 
   0.9785    0.9785 

##joint dataframe data

RNA normalized 24 hour cTNNI / LDH

Calcium data at 24 hours

Beat Rate

Mean amplitude

Full-Width-half max

Rising Slope

Decay slope

k means plot


R version 4.2.2 (2022-10-31 ucrt)
Platform: x86_64-w64-mingw32/x64 (64-bit)
Running under: Windows 10 x64 (build 19045)

Matrix products: default

locale:
[1] LC_COLLATE=English_United States.utf8 
[2] LC_CTYPE=English_United States.utf8   
[3] LC_MONETARY=English_United States.utf8
[4] LC_NUMERIC=C                          
[5] LC_TIME=English_United States.utf8    

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
 [1] ggalt_0.4.0        RColorBrewer_1.1-3 ggsignif_0.6.4     zoo_1.8-11        
 [5] lubridate_1.9.2    forcats_1.0.0      stringr_1.5.0      dplyr_1.1.0       
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[13] tidyverse_2.0.0    rstatix_0.7.2      ggpubr_0.6.0       ggplot2_3.4.1     
[17] readxl_1.4.2       workflowr_1.7.0   

loaded via a namespace (and not attached):
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 [5] httr_1.4.5         rprojroot_2.0.3    tools_4.2.2        backports_1.4.1   
 [9] bslib_0.4.2        utf8_1.2.3         R6_2.5.1           KernSmooth_2.23-20
[13] mgcv_1.8-42        colorspace_2.1-0   withr_2.5.0        tidyselect_1.2.0  
[17] processx_3.8.0     extrafontdb_1.0    bit_4.0.5          compiler_4.2.2    
[21] git2r_0.31.0       cli_3.6.0          labeling_0.4.2     sass_0.4.5        
[25] scales_1.2.1       proj4_1.0-12       callr_3.7.3        digest_0.6.31     
[29] rmarkdown_2.20     pkgconfig_2.0.3    htmltools_0.5.4    extrafont_0.19    
[33] fastmap_1.1.1      highr_0.10         maps_3.4.1         rlang_1.0.6       
[37] rstudioapi_0.14    jquerylib_0.1.4    farver_2.1.1       generics_0.1.3    
[41] jsonlite_1.8.4     vroom_1.6.1        car_3.1-1          magrittr_2.0.3    
[45] Matrix_1.5-3       Rcpp_1.0.10        munsell_0.5.0      fansi_1.0.4       
[49] abind_1.4-5        lifecycle_1.0.3    stringi_1.7.12     whisker_0.4.1     
[53] yaml_2.3.7         carData_3.0-5      MASS_7.3-58.2      grid_4.2.2        
[57] parallel_4.2.2     promises_1.2.0.1   crayon_1.5.2       lattice_0.20-45   
[61] splines_4.2.2      hms_1.1.2          knitr_1.42         ps_1.7.2          
[65] pillar_1.8.1       glue_1.6.2         evaluate_0.20      getPass_0.2-2     
[69] vctrs_0.5.2        tzdb_0.3.0         httpuv_1.6.9       Rttf2pt1_1.3.12   
[73] cellranger_1.1.0   gtable_0.3.1       cachem_1.0.7       xfun_0.37         
[77] broom_1.0.3        later_1.3.0        timechange_0.2.0   ellipsis_0.3.2