LDL - Liver
sheng Qian
2021-2-6
Last updated: 2022-02-22
Checks: 6 1
Knit directory: cTWAS_analysis/
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| Rmd | fe3e6a4 | sq-96 | 2022-02-22 | update |
Weight QC
#number of imputed weights
nrow(qclist_all)[1] 21553#number of imputed weights by chromosome
table(qclist_all$chr)
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
2079 1523 1276 769 878 1116 1176 701 854 931 1344 1116 391 772 677 1085
17 18 19 20 21 22
1462 258 1524 659 262 700 #number of imputed weights without missing variants
sum(qclist_all$nmiss==0)[1] 19937#proportion of imputed weights without missing variants
mean(qclist_all$nmiss==0)[1] 0.925ctwas_gene_res$genename <- NA
ctwas_gene_res$intron_id <- ctwas_gene_res$id
library(EnsDb.Hsapiens.v79)Loading required package: ensembldbLoading required package: BiocGenericsLoading required package: parallel
Attaching package: 'BiocGenerics'The following objects are masked from 'package:parallel':
clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
clusterExport, clusterMap, parApply, parCapply, parLapply,
parLapplyLB, parRapply, parSapply, parSapplyLBThe following objects are masked from 'package:stats':
IQR, mad, sd, var, xtabsThe following objects are masked from 'package:base':
anyDuplicated, append, as.data.frame, basename, cbind, colnames,
dirname, do.call, duplicated, eval, evalq, Filter, Find, get, grep,
grepl, intersect, is.unsorted, lapply, Map, mapply, match, mget,
order, paste, pmax, pmax.int, pmin, pmin.int, Position, rank,
rbind, Reduce, rownames, sapply, setdiff, sort, table, tapply,
union, unique, unsplit, which, which.max, which.minLoading required package: GenomicRangesLoading required package: stats4Loading required package: S4Vectors
Attaching package: 'S4Vectors'The following object is masked from 'package:base':
expand.gridLoading required package: IRangesLoading required package: GenomeInfoDbLoading required package: GenomicFeaturesLoading required package: AnnotationDbiLoading required package: BiobaseWelcome to Bioconductor
Vignettes contain introductory material; view with
'browseVignettes()'. To cite Bioconductor, see
'citation("Biobase")', and for packages 'citation("pkgname")'.Loading required package: AnnotationFilter
Attaching package: 'ensembldb'The following object is masked from 'package:stats':
filteredb <- EnsDb.Hsapiens.v79
for(i in 1:dim(ctwas_gene_res)[1]){
print(i)
info <- strsplit(ctwas_gene_res[i,'id'], "_")[[1]]
chr = as.numeric(info[2])
start.pos = as.numeric(info[3])
end.pos = as.numeric(info[4])
grf <- GRangesFilter(GRanges(chr, ranges = IRanges(start.pos, end.pos)), type = "any")
gn <- genes(edb, filter = grf)
if(length(gn$gene_id)!=0){
ctwas_gene_res[i,"id"] <- gn$gene_id[1]
ctwas_gene_res[i,"genename"] <- gn$gene_name[1]
ctwas_gene_res[i,"type"] <- gn$gene_biotype[1]
}
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[1] 21553#add z scores to results
load(paste0(results_dir, "/", analysis_id, "_expr_z_gene.Rd"))
ctwas_gene_res$z <- z_gene[ctwas_gene_res$intron_id,]$z
z_snp <- z_snp[z_snp$id %in% ctwas_snp_res$id,]
ctwas_snp_res$z <- z_snp$z[match(ctwas_snp_res$id, z_snp$id)]
#merge gene and snp results with added information
ctwas_snp_res$genename=NA
ctwas_snp_res$gene_type=NA
ctwas_snp_res$intron_id=NA
ctwas_res <- rbind(ctwas_gene_res,
ctwas_snp_res[,colnames(ctwas_gene_res)])
#get number of eQTL for geens
num_eqtl <- c()
for (i in 1:22){
load(paste0(results_dir, "/", analysis_id, "_expr_chr", i, ".exprqc.Rd"))
num_eqtl <- c(num_eqtl, unlist(lapply(wgtlist, nrow)))
}
ctwas_gene_res$num_eqtl <- num_eqtl[ctwas_gene_res$intron_id]
#store columns to report
report_cols <- colnames(ctwas_gene_res)[!(colnames(ctwas_gene_res) %in% c("type", "region_tag1", "region_tag2", "cs_index", "gene_type", "z_flag", "id", "chrom", "pos"))]
first_cols <- c("genename", "region_tag")
report_cols <- c(first_cols, report_cols[!(report_cols %in% first_cols)])
report_cols_snps <- c("id", report_cols[-1])
report_cols_snps <- report_cols_snps[!(report_cols_snps %in% "num_eqtl")]
#get number of SNPs from s1 results; adjust for thin argument
ctwas_res_s1 <- data.table::fread(paste0(results_dir, "/", analysis_id, "_ctwas.s1.susieIrss.txt"))
n_snps <- sum(ctwas_res_s1$type=="SNP")/thin
rm(ctwas_res_s1)Check convergence of parameters
#estimated group prior
estimated_group_prior <- group_prior_rec[,ncol(group_prior_rec)]
names(estimated_group_prior) <- c("gene", "snp")
estimated_group_prior["snp"] <- estimated_group_prior["snp"]*thin #adjust parameter to account for thin argument
print(estimated_group_prior) gene snp
1.259e-02 6.141e-05 #estimated group prior variance
estimated_group_prior_var <- group_prior_var_rec[,ncol(group_prior_var_rec)]
names(estimated_group_prior_var) <- c("gene", "snp")
print(estimated_group_prior_var) gene snp
13.57 36.27 #report sample size
print(sample_size)[1] 343621#report group size
group_size <- c(nrow(ctwas_gene_res), n_snps)
print(group_size)[1] 21553 8696600#estimated group PVE
estimated_group_pve <- estimated_group_prior_var*estimated_group_prior*group_size/sample_size #check PVE calculation
names(estimated_group_pve) <- c("gene", "snp")
print(estimated_group_pve) gene snp
0.01072 0.05637 #compare sum(PIP*mu2/sample_size) with above PVE calculation
c(sum(ctwas_gene_res$PVE),sum(ctwas_snp_res$PVE))[1] 0.04198 0.19087Genes with highest PIPs
genename region_tag susie_pip mu2 PVE
21059 TXNL4B 16_38 0.9963 273.80 7.939e-04
20786 RP11-44M6.7 7_61 0.9924 30.57 8.828e-05
21019 ITGAL 16_24 0.9628 21.91 6.139e-05
21422 BCAT2 19_34 0.9590 28.03 7.822e-05
20840 KIF13B 8_28 0.9474 23.61 6.508e-05
20881 ST3GAL4 11_77 0.9465 76.84 2.117e-04
20569 USP53 4_77 0.9393 26.07 7.127e-05
21414 SPHK2 19_33 0.9143 42.17 1.122e-04
20891 PHC1 12_9 0.9060 37.06 9.771e-05
21287 CYP4F12 19_13 0.8950 36.30 9.456e-05
20504 SPRED2 2_42 0.8624 22.18 5.567e-05
20526 UGT1A8 2_137 0.8109 30.91 7.295e-05
20919 R3HDM2 12_36 0.8039 36.34 8.500e-05
8089 BRI3 7_60 0.7922 24.51 5.652e-05
6609 ZSCAN31 6_22 0.7912 24.69 5.686e-05
18613 ZNF227 19_30 0.7911 30.28 6.970e-05
410 KDM1A 1_16 0.7908 20.77 4.779e-05
7568 RAC1 7_9 0.7885 25.07 5.752e-05
21097 FLOT2 17_17 0.7856 26.89 6.149e-05
21368 STRN4 19_33 0.7786 18.83 4.267e-05
intron_id z num_eqtl
21059 intron_16_72057466_72058254 21.899 2
20786 intron_7_100575304_100575705 5.325 2
21019 intron_16_30505462_30506715 -4.428 1
21422 intron_19_48800297_48810984 -5.197 1
20840 intron_8_29092878_29099133 -4.718 1
20881 intron_11_126356302_126406096 -12.159 2
20569 intron_4_119217673_119220116 4.888 1
21414 intron_19_48624430_48625891 8.721 1
20891 intron_12_8921750_8922633 6.156 1
21287 intron_19_15685197_15696430 -5.938 2
20504 intron_2_65314169_65316734 -4.438 1
20526 intron_2_233761151_233767034 5.450 1
20919 intron_12_57395818_57430720 -5.818 1
8089 intron_7_98252528_98282351 4.827 2
6609 intron_6_28327533_28329303 2.711 1
18613 intron_19_44213244_44217791 4.698 1
410 intron_1_23030634_23050387 4.158 1
7568 intron_7_6400188_6401868 4.635 1
21097 intron_17_28889026_28897526 -3.738 1
21368 intron_19_46738888_46746149 2.732 1Genes with largest effect sizes
genename region_tag susie_pip mu2 PVE
18625 BCL3 19_31 0.000e+00 1095.1 0.000e+00
18623 CTB-171A8.1 19_31 0.000e+00 1047.0 0.000e+00
18624 CTB-171A8.1 19_31 0.000e+00 1047.0 0.000e+00
18634 PVRL2 19_31 0.000e+00 794.9 0.000e+00
18635 PVRL2 19_31 0.000e+00 794.9 0.000e+00
21247 LDLR 19_9 1.216e-08 559.9 1.982e-11
917 DHCR24 1_34 8.490e-05 338.5 8.364e-08
21244 SMARCA4 19_9 0.000e+00 298.5 0.000e+00
21059 TXNL4B 16_38 9.963e-01 273.8 7.939e-04
21062 TXNL4B 16_38 3.242e-02 273.2 2.577e-05
21065 TXNL4B 16_38 2.177e-02 255.5 1.619e-05
960 DOCK7 1_39 3.148e-02 232.2 2.127e-05
958 DOCK7 1_39 3.069e-02 232.1 2.073e-05
957 DOCK7 1_39 2.585e-02 230.8 1.737e-05
1201 SARS 1_67 9.267e-08 225.5 6.081e-11
21246 LDLR 19_9 2.509e-06 198.7 1.451e-09
5892 POLK 5_44 2.684e-02 197.2 1.540e-05
5896 POC5 5_44 1.357e-02 189.1 7.466e-06
18358 GATAD2A 19_15 1.365e-02 183.3 7.281e-06
17111 ABCA6 17_39 2.981e-02 182.3 1.581e-05
intron_id z num_eqtl
18625 intron_19_44749046_44751227 35.15 2
18623 intron_19_44672964_44673712 30.68 1
18624 intron_19_44672964_44676271 -30.68 1
18634 intron_19_44874478_44878553 16.87 1
18635 intron_19_44874478_44882211 -16.87 1
21247 intron_19_11120522_11123174 26.01 2
917 intron_1_54883773_54886889 11.28 1
21244 intron_19_11039553_11041307 15.44 1
21059 intron_16_72057466_72058254 21.90 2
21062 intron_16_72058355_72059114 21.57 2
21065 intron_16_72074385_72075145 -20.56 3
960 intron_1_62663130_62688227 16.23 1
958 intron_1_62584878_62586507 16.22 1
957 intron_1_62457705_62473982 -16.19 1
1201 intron_1_109229572_109230878 -6.03 1
21246 intron_19_11116212_11120092 16.87 1
5892 intron_5_75587058_75590344 -16.85 1
5896 intron_5_75690562_75692396 -16.09 1
18358 intron_19_19457229_19465340 12.85 1
17111 intron_17_69084507_69085028 -2.22 1Genes with highest PVE
genename region_tag susie_pip mu2 PVE
21059 TXNL4B 16_38 0.9963 273.80 7.939e-04
20881 ST3GAL4 11_77 0.9465 76.84 2.117e-04
20961 ALDH1A2 15_26 0.7321 62.70 1.336e-04
16321 ASGR1 17_6 0.5170 84.16 1.266e-04
16322 ASGR1 17_6 0.5170 84.16 1.266e-04
21414 SPHK2 19_33 0.9143 42.17 1.122e-04
21101 PGS1 17_45 0.7143 48.72 1.013e-04
20891 PHC1 12_9 0.9060 37.06 9.771e-05
21287 CYP4F12 19_13 0.8950 36.30 9.456e-05
20786 RP11-44M6.7 7_61 0.9924 30.57 8.828e-05
20919 R3HDM2 12_36 0.8039 36.34 8.500e-05
16354 AC113189.5 17_7 0.7625 36.87 8.182e-05
445 TMEM57 1_18 0.2771 99.08 7.991e-05
21422 BCAT2 19_34 0.9590 28.03 7.822e-05
7954 POR 7_48 0.7419 34.19 7.383e-05
20526 UGT1A8 2_137 0.8109 30.91 7.295e-05
446 TMEM57 1_18 0.2499 98.82 7.186e-05
20569 USP53 4_77 0.9393 26.07 7.127e-05
6085 C5orf56 5_79 0.7319 33.09 7.047e-05
18613 ZNF227 19_30 0.7911 30.28 6.970e-05
intron_id z num_eqtl
21059 intron_16_72057466_72058254 21.899 2
20881 intron_11_126356302_126406096 -12.159 2
20961 intron_15_58431776_58431993 -7.783 1
16321 intron_17_7176901_7176981 9.645 1
16322 intron_17_7176901_7177003 -9.645 1
21414 intron_19_48624430_48625891 8.721 1
21101 intron_17_78378808_78392476 -7.141 1
20891 intron_12_8921750_8922633 6.156 1
21287 intron_19_15685197_15696430 -5.938 2
20786 intron_7_100575304_100575705 5.325 2
20919 intron_12_57395818_57430720 -5.818 1
16354 intron_17_7582163_7582900 1.838 2
445 intron_1_25456831_25458391 -10.599 1
21422 intron_19_48800297_48810984 -5.197 1
7954 intron_7_75981606_75982224 -5.707 2
20526 intron_2_233761151_233767034 5.450 1
446 intron_1_25458892_25484116 -10.587 1
20569 intron_4_119217673_119220116 4.888 1
6085 intron_5_132419940_132449650 5.433 1
18613 intron_19_44213244_44217791 4.698 1Genes with largest z scores
genename region_tag susie_pip mu2 PVE
18625 BCL3 19_31 0.000e+00 1095.1 0.000e+00
18623 CTB-171A8.1 19_31 0.000e+00 1047.0 0.000e+00
18624 CTB-171A8.1 19_31 0.000e+00 1047.0 0.000e+00
21247 LDLR 19_9 1.216e-08 559.9 1.982e-11
21059 TXNL4B 16_38 9.963e-01 273.8 7.939e-04
21062 TXNL4B 16_38 3.242e-02 273.2 2.577e-05
21065 TXNL4B 16_38 2.177e-02 255.5 1.619e-05
1200 SARS 1_67 1.968e-07 149.5 8.559e-11
18634 PVRL2 19_31 0.000e+00 794.9 0.000e+00
18635 PVRL2 19_31 0.000e+00 794.9 0.000e+00
21246 LDLR 19_9 2.509e-06 198.7 1.451e-09
5892 POLK 5_44 2.684e-02 197.2 1.540e-05
960 DOCK7 1_39 3.148e-02 232.2 2.127e-05
958 DOCK7 1_39 3.069e-02 232.1 2.073e-05
957 DOCK7 1_39 2.585e-02 230.8 1.737e-05
5896 POC5 5_44 1.357e-02 189.1 7.466e-06
5893 POLK 5_44 1.086e-02 136.8 4.324e-06
21064 TXNL4B 16_38 6.148e-03 127.1 2.275e-06
21244 SMARCA4 19_9 0.000e+00 298.5 0.000e+00
21060 TXNL4B 16_38 6.509e-03 106.4 2.016e-06
intron_id z num_eqtl
18625 intron_19_44749046_44751227 35.15 2
18623 intron_19_44672964_44673712 30.68 1
18624 intron_19_44672964_44676271 -30.68 1
21247 intron_19_11120522_11123174 26.01 2
21059 intron_16_72057466_72058254 21.90 2
21062 intron_16_72058355_72059114 21.57 2
21065 intron_16_72074385_72075145 -20.56 3
1200 intron_1_109214128_109214547 18.21 1
18634 intron_19_44874478_44878553 16.87 1
18635 intron_19_44874478_44882211 -16.87 1
21246 intron_19_11116212_11120092 16.87 1
5892 intron_5_75587058_75590344 -16.85 1
960 intron_1_62663130_62688227 16.23 1
958 intron_1_62584878_62586507 16.22 1
957 intron_1_62457705_62473982 -16.19 1
5896 intron_5_75690562_75692396 -16.09 1
5893 intron_5_75594049_75596222 15.58 1
21064 intron_16_72059188_72060112 -15.52 2
21244 intron_19_11039553_11041307 15.44 1
21060 intron_16_72057466_72059143 -13.62 1Comparing z scores and PIPs
[1] 0.01707 genename region_tag susie_pip mu2 PVE
18625 BCL3 19_31 0.000e+00 1095.1 0.000e+00
18623 CTB-171A8.1 19_31 0.000e+00 1047.0 0.000e+00
18624 CTB-171A8.1 19_31 0.000e+00 1047.0 0.000e+00
21247 LDLR 19_9 1.216e-08 559.9 1.982e-11
21059 TXNL4B 16_38 9.963e-01 273.8 7.939e-04
21062 TXNL4B 16_38 3.242e-02 273.2 2.577e-05
21065 TXNL4B 16_38 2.177e-02 255.5 1.619e-05
1200 SARS 1_67 1.968e-07 149.5 8.559e-11
18634 PVRL2 19_31 0.000e+00 794.9 0.000e+00
18635 PVRL2 19_31 0.000e+00 794.9 0.000e+00
21246 LDLR 19_9 2.509e-06 198.7 1.451e-09
5892 POLK 5_44 2.684e-02 197.2 1.540e-05
960 DOCK7 1_39 3.148e-02 232.2 2.127e-05
958 DOCK7 1_39 3.069e-02 232.1 2.073e-05
957 DOCK7 1_39 2.585e-02 230.8 1.737e-05
5896 POC5 5_44 1.357e-02 189.1 7.466e-06
5893 POLK 5_44 1.086e-02 136.8 4.324e-06
21064 TXNL4B 16_38 6.148e-03 127.1 2.275e-06
21244 SMARCA4 19_9 0.000e+00 298.5 0.000e+00
21060 TXNL4B 16_38 6.509e-03 106.4 2.016e-06
intron_id z num_eqtl
18625 intron_19_44749046_44751227 35.15 2
18623 intron_19_44672964_44673712 30.68 1
18624 intron_19_44672964_44676271 -30.68 1
21247 intron_19_11120522_11123174 26.01 2
21059 intron_16_72057466_72058254 21.90 2
21062 intron_16_72058355_72059114 21.57 2
21065 intron_16_72074385_72075145 -20.56 3
1200 intron_1_109214128_109214547 18.21 1
18634 intron_19_44874478_44878553 16.87 1
18635 intron_19_44874478_44882211 -16.87 1
21246 intron_19_11116212_11120092 16.87 1
5892 intron_5_75587058_75590344 -16.85 1
960 intron_1_62663130_62688227 16.23 1
958 intron_1_62584878_62586507 16.22 1
957 intron_1_62457705_62473982 -16.19 1
5896 intron_5_75690562_75692396 -16.09 1
5893 intron_5_75594049_75596222 15.58 1
21064 intron_16_72059188_72060112 -15.52 2
21244 intron_19_11039553_11041307 15.44 1
21060 intron_16_72057466_72059143 -13.62 1GO enrichment analysis for genes with PIP>0.5
#number of genes for gene set enrichment
length(genes)[1] 62Uploading data to Enrichr... Done.
Querying GO_Biological_Process_2021... Done.
Querying GO_Cellular_Component_2021... Done.
Querying GO_Molecular_Function_2021... Done.
Parsing results... Done.
[1] "GO_Biological_Process_2021"
[1] Term Overlap Adjusted.P.value Genes
<0 rows> (or 0-length row.names)
[1] "GO_Cellular_Component_2021"
[1] Term Overlap Adjusted.P.value Genes
<0 rows> (or 0-length row.names)
[1] "GO_Molecular_Function_2021"
[1] Term Overlap Adjusted.P.value Genes
<0 rows> (or 0-length row.names)DisGeNET enrichment analysis for genes with PIP>0.5
Warning in disease_enrichment(entities = genes, vocabulary = "HGNC", database =
"CURATED"): Removing duplicates from input list. Description
111 Trimethylaminuria
112 Complement component 5 deficiency
195 CARDIOMYOPATHY, FAMILIAL HYPERTROPHIC, 3 (disorder)
200 LEOPARD SYNDROME 2
201 Noonan Syndrome 5
205 Cardiomyopathy, Dilated, 1y
210 ANTLEY-BIXLER SYNDROME WITH GENITAL ANOMALIES AND DISORDERED STEROIDOGENESIS
225 CARDIOMYOPATHY, DILATED, 1NN
227 CONGENITAL DISORDER OF GLYCOSYLATION, TYPE IIp
228 CLEFT PALATE, PSYCHOMOTOR RETARDATION, AND DISTINCTIVE FACIAL FEATURES
FDR Ratio BgRatio
111 0.05953 1/29 1/9703
112 0.05953 1/29 1/9703
195 0.05953 1/29 1/9703
200 0.05953 1/29 1/9703
201 0.05953 1/29 1/9703
205 0.05953 1/29 1/9703
210 0.05953 1/29 1/9703
225 0.05953 1/29 1/9703
227 0.05953 1/29 1/9703
228 0.05953 1/29 1/9703WebGestalt enrichment analysis for genes with PIP>0.5
Loading the functional categories...
Loading the ID list...
Loading the reference list...
Performing the enrichment analysis...Warning in oraEnrichment(interestGeneList, referenceGeneList, geneSet, minNum =
minNum, : No significant gene set is identified based on FDR 0.05!NULLPIP Manhattan Plot
Warning: ggrepel: 17 unlabeled data points (too many overlaps). Consider
increasing max.overlaps
Sensitivity, specificity and precision for silver standard genes
New names:
* `` -> ...4
* `` -> ...5#number of genes in known annotations
print(length(known_annotations))[1] 69#number of genes in known annotations with imputed expression
print(sum(known_annotations %in% ctwas_gene_res$genename))[1] 34#significance threshold for TWAS
print(sig_thresh)[1] 4.723#number of ctwas genes
length(ctwas_genes)[1] 13#number of TWAS genes
length(twas_genes)[1] 368#show novel genes (ctwas genes with not in TWAS genes)
ctwas_gene_res[ctwas_gene_res$genename %in% novel_genes,report_cols] genename region_tag susie_pip mu2 PVE intron_id
20504 SPRED2 2_42 0.86236 22.18 5.567e-05 intron_2_65314169_65316734
20505 SPRED2 2_42 0.15798 32.23 1.482e-05 intron_2_65332051_65334605
20840 KIF13B 8_28 0.94736 23.61 6.508e-05 intron_8_29092878_29099133
21017 ITGAL 16_24 0.03733 12.34 1.341e-06 intron_16_30489155_30489254
21018 ITGAL 16_24 0.03733 12.34 1.341e-06 intron_16_30489386_30494212
21019 ITGAL 16_24 0.96283 21.91 6.139e-05 intron_16_30505462_30506715
z num_eqtl
20504 -4.438 1
20505 -4.003 1
20840 -4.718 1
21017 -2.590 1
21018 2.590 1
21019 -4.428 1#sensitivity / recall
print(sensitivity) ctwas TWAS
0.0000 0.1014 #specificity
print(specificity) ctwas TWAS
0.9974 0.9656 #precision / PPV
print(precision) ctwas TWAS
0.00000 0.01902 
sessionInfo()R version 3.6.1 (2019-07-05)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Scientific Linux 7.4 (Nitrogen)
Matrix products: default
BLAS/LAPACK: /software/openblas-0.2.19-el7-x86_64/lib/libopenblas_haswellp-r0.2.19.so
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
[3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8
[5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8
[7] LC_PAPER=en_US.UTF-8 LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
attached base packages:
[1] stats4 parallel stats graphics grDevices utils datasets
[8] methods base
other attached packages:
[1] readxl_1.3.1 forcats_0.5.1
[3] stringr_1.4.0 dplyr_1.0.7
[5] purrr_0.3.4 readr_2.1.1
[7] tidyr_1.1.4 tidyverse_1.3.1
[9] tibble_3.1.6 WebGestaltR_0.4.4
[11] disgenet2r_0.99.2 enrichR_3.0
[13] cowplot_1.0.0 ggplot2_3.3.5
[15] EnsDb.Hsapiens.v79_2.99.0 ensembldb_2.8.0
[17] AnnotationFilter_1.8.0 GenomicFeatures_1.36.3
[19] AnnotationDbi_1.46.0 Biobase_2.44.0
[21] GenomicRanges_1.36.1 GenomeInfoDb_1.20.0
[23] IRanges_2.18.1 S4Vectors_0.22.1
[25] BiocGenerics_0.30.0 workflowr_1.6.2
loaded via a namespace (and not attached):
[1] ggbeeswarm_0.6.0 colorspace_2.0-2
[3] rjson_0.2.20 ellipsis_0.3.2
[5] rprojroot_2.0.2 XVector_0.24.0
[7] fs_1.5.2 rstudioapi_0.13
[9] farver_2.1.0 ggrepel_0.9.1
[11] bit64_4.0.5 lubridate_1.8.0
[13] fansi_0.5.0 xml2_1.3.3
[15] codetools_0.2-16 doParallel_1.0.16
[17] cachem_1.0.6 knitr_1.36
[19] jsonlite_1.7.2 apcluster_1.4.8
[21] Cairo_1.5-12.2 Rsamtools_2.0.0
[23] broom_0.7.10 dbplyr_2.1.1
[25] compiler_3.6.1 httr_1.4.2
[27] backports_1.4.1 assertthat_0.2.1
[29] Matrix_1.2-18 fastmap_1.1.0
[31] lazyeval_0.2.2 cli_3.1.0
[33] later_0.8.0 htmltools_0.5.2
[35] prettyunits_1.1.1 tools_3.6.1
[37] igraph_1.2.10 gtable_0.3.0
[39] glue_1.5.1 GenomeInfoDbData_1.2.1
[41] reshape2_1.4.4 doRNG_1.8.2
[43] Rcpp_1.0.7 cellranger_1.1.0
[45] jquerylib_0.1.4 vctrs_0.3.8
[47] Biostrings_2.52.0 svglite_1.2.2
[49] rtracklayer_1.44.4 iterators_1.0.13
[51] xfun_0.29 rvest_1.0.2
[53] lifecycle_1.0.1 rngtools_1.5.2
[55] XML_3.99-0.3 zlibbioc_1.30.0
[57] scales_1.1.1 vroom_1.5.7
[59] hms_1.1.1 promises_1.0.1
[61] ProtGenerics_1.16.0 SummarizedExperiment_1.14.1
[63] yaml_2.2.1 curl_4.3.2
[65] memoise_2.0.1 ggrastr_1.0.1
[67] gdtools_0.1.9 biomaRt_2.40.1
[69] stringi_1.7.6 RSQLite_2.2.8
[71] highr_0.9 foreach_1.5.1
[73] BiocParallel_1.18.0 rlang_0.4.12
[75] pkgconfig_2.0.3 matrixStats_0.57.0
[77] bitops_1.0-7 evaluate_0.14
[79] lattice_0.20-38 GenomicAlignments_1.20.1
[81] labeling_0.4.2 bit_4.0.4
[83] tidyselect_1.1.1 plyr_1.8.6
[85] magrittr_2.0.1 R6_2.5.1
[87] generics_0.1.1 DelayedArray_0.10.0
[89] DBI_1.1.1 haven_2.4.3
[91] pillar_1.6.4 whisker_0.3-2
[93] withr_2.4.3 RCurl_1.98-1.5
[95] modelr_0.1.8 crayon_1.4.2
[97] utf8_1.2.2 tzdb_0.2.0
[99] rmarkdown_2.11 progress_1.2.2
[101] grid_3.6.1 data.table_1.14.2
[103] blob_1.2.2 git2r_0.26.1
[105] reprex_2.0.1 digest_0.6.29
[107] httpuv_1.5.1 munsell_0.5.0
[109] beeswarm_0.2.3 vipor_0.4.5