Last updated: 2018-06-30

workflowr checks: (Click a bullet for more information)

  • ✔ R Markdown file: up-to-date

    Great! Since the R Markdown file has been committed to the Git repository, you know the exact version of the code that produced these results.

  • ✔ Environment: empty

    Great job! The global environment was empty. Objects defined in the global environment can affect the analysis in your R Markdown file in unknown ways. For reproduciblity it’s best to always run the code in an empty environment.

  • ✔ Seed: set.seed(20180626)

    The command set.seed(20180626) was run prior to running the code in the R Markdown file. Setting a seed ensures that any results that rely on randomness, e.g. subsampling or permutations, are reproducible.

  • ✔ Session information: recorded

    Great job! Recording the operating system, R version, and package versions is critical for reproducibility.

  • ✔ Repository version: 64b30ca

    Great! You are using Git for version control. Tracking code development and connecting the code version to the results is critical for reproducibility. The version displayed above was the version of the Git repository at the time these results were generated.

    Note that you need to be careful to ensure that all relevant files for the analysis have been committed to Git prior to generating the results (you can use wflow_publish or wflow_git_commit). workflowr only checks the R Markdown file, but you know if there are other scripts or data files that it depends on. Below is the status of the Git repository when the results were generated: 
    
Ignored files:
    Ignored:    .Rproj.user/

Unstaged changes:
    Modified:   analysis/index.Rmd
    Note that any generated files, e.g. HTML, png, CSS, etc., are not included in this status report because it is ok for generated content to have uncommitted changes.
Expand here to see past versions:
    File Version Author Date Message
    Rmd 64b30ca Xiang Zhu 2018-06-30 wflow_publish(“uc_2015.Rmd”)

Input data

Results below were generated from the GWAS summary statistics published in the paper “Association Analyses Identify 38 Susceptibility Loci for Inflammatory Bowel Disease and Highlight Shared Genetic Risk Across Populations” (Nature Genetics, 2015). The summary data file is available at https://www.ibdgenetics.org/downloads.html.

Analysis results

Enrichment analyses are summarized by the following three quantities.

  • BF: Bayes factor comparing the enrichment model against the baseline model;
  • Outside \(\pi\): proportion of trait-associated SNPs that are “outside” the gene set;
  • Inside \(\pi\): proportion of trait-associated SNPs that are “inside” the gene set.

The first quantity reflects the significance of enrichment, whereas the last two capture the magnitude of enrichment. For each gene set, we report these three quantities in the last three columns of tables below, on log 10 scale.

Biological pathways

Tissue highly expressed genes

Tissue selectively expressed genes

Cluster distinctively expressed genes

The relationship between tissues and clusters is shown in Figure 1 of Dey et al. (2017); see below.

Session information

R version 3.5.0 (2018-04-23)
Platform: x86_64-apple-darwin15.6.0 (64-bit)
Running under: macOS High Sierra 10.13.5

Matrix products: default
BLAS: /Library/Frameworks/R.framework/Versions/3.5/Resources/lib/libRblas.0.dylib
LAPACK: /Library/Frameworks/R.framework/Versions/3.5/Resources/lib/libRlapack.dylib

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
[1] DT_0.4         plyr_1.8.4     dplyr_0.7.5    R.matlab_3.6.1

loaded via a namespace (and not attached):
 [1] Rcpp_0.12.17      compiler_3.5.0    pillar_1.2.3     
 [4] later_0.7.3       git2r_0.21.0      workflowr_1.0.1  
 [7] bindr_0.1.1       R.methodsS3_1.7.1 R.utils_2.6.0    
[10] tools_3.5.0       digest_0.6.15     jsonlite_1.5     
[13] evaluate_0.10.1   tibble_1.4.2      pkgconfig_2.0.1  
[16] rlang_0.2.1       shiny_1.1.0       crosstalk_1.0.0  
[19] yaml_2.1.19       bindrcpp_0.2.2    stringr_1.3.1    
[22] knitr_1.20        htmlwidgets_1.2   rprojroot_1.3-2  
[25] tidyselect_0.2.4  glue_1.2.0        R6_2.2.2         
[28] rmarkdown_1.10    purrr_0.2.5       magrittr_1.5     
[31] whisker_0.3-2     backports_1.1.2   promises_1.0.1   
[34] htmltools_0.3.6   assertthat_0.2.0  mime_0.5         
[37] xtable_1.8-2      httpuv_1.4.4.1    stringi_1.2.3    
[40] R.oo_1.22.0

This reproducible R Markdown analysis was created with workflowr 1.0.1