Last updated: 2020-08-14

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Knit directory: Embryoid_Body_Pilot_Workflowr/analysis/

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Rmd 8fcb48a KLRhodes 2020-08-14 wflow_publish(“analysis/index.Rmd”)
html 508974d KLRhodes 2020-08-14 Build site.
Rmd 7a220f3 KLRhodes 2020-08-14 wflow_publish(“analysis/index.Rmd”)
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Rmd 87c7292 KLRhodes 2020-08-04 Start workflowr project.

Welcome to my research website.

This site contains analyses performed on human Embryoid bodies, spheroids of spontaneously differentiating cells generated from induced pluripotent stem cells.

Single Cell RNA-seq data was collected using the 10x platform.

After running cell ranger,

Preprocessing, QC, and filtering 1. Run EmptyDrops, Add Metadata to Seurat object from each 10x lane 2. Filter and run SCTransform on each 10x lane -Batch1Lane1 -Batch1Lane3 -Batch1Lane4 -Batch1Lane5 -Batch1Lane6 -Batch1Lane7 -Batch1Lane8 -Batch1Lane9 -Batch2Lane1 -Batch2Lane2 -Batch2Lane3 -Batch2Lane4 -Batch3Lane1 -Batch3Lane2 -Batch3Lane3 -Batch3Lane4