Last updated: 2022-01-18

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Knit directory: HenriqueDGen/

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Introduction

Phenotypic data

Descriptive and deviance analysis were performed to verify the distribution and the genotypic effects for resistance to foliar diseases. In this work we used a natural logarithm transformation for Affected area by Anthracnosis at the innoculated area at stem in cassava plants (AnAr).

suppressMessages(library(tidyverse))
library(here)
here() starts at /Users/lbd54/Documents/GitHub/HenriqueDGen
suppressMessages(library(reactable))

# Data read by R using here package to allow the read in any computer with different directory path
PhenoData <- readRDS(here::here("data", "DadosFenotipicos.RDS"))
# Add the control information for the Mixed Models analysis
control <- names(table(PhenoData$accession_name)[table(PhenoData$accession_name) > 30])
PhenoData$control <- ifelse(PhenoData$accession_name %in% control, PhenoData$accession_name, "999") 
PhenoData$new <- ifelse(PhenoData$accession_name %in% control, 0, 1)

# Change the Disease names to english abbreviations
colnames(PhenoData)[7:11] <- c("Anth", "WhLS", "BrLS", "BlLS", "AnAr")

# Natural logarithm transformation for Anthracnosis Area
PhenoData$AnAr <- log(PhenoData$AnAr)

Table 1. Phenotypic data for resistance to foliar disease evaluated at the Cassava Germplasm Bank of EMBRAPA, data collected in 2021.

Anthracnosis (Anth), White Leaf spot (WhLS), Brown Leaf spot (BrLS), Blight Leaf Spot (BlLS), Affected area by Anthracnosis at the innoculated area at stem in cassava plants (AnAr).

lnfUtl <- colnames(PhenoData)[c(1:4, 12:13)]
PhenoData$talhao_number <- NULL
PhenoData$Idade <- NULL
traits <- colnames(PhenoData)[!colnames(PhenoData) %in% lnfUtl]
PhenoData2 <- PhenoData %>% gather(key = traits, value = Y, -all_of(lnfUtl))

Table 2. Data entry for the descriptive analysis and mixed models for resistance of foliar disease in cassava.

Anthracnosis (Anth), White Leaf spot (WhLS), Brown Leaf spot (BrLS), Blight Leaf Spot (BlLS), Affected area by Anthracnosis at the innoculated area at stem in cassava plants (AnAr).

saveRDS(PhenoData2, here::here("output", "DadosFenotipicosv2.RDS"))

Atividades a finalizar nesta seção

Característica Sigla
Genotipos - 1˚coluna Genotipos.BGM
Altura.de.planta….CO_334.0000018 AP
Altura.de.planta.sem.folha….CO_334.0000125 APsF
PTR - Produtividade Total de Raizes PTR
PRC - Produtividade de Raiz Comercial PRC
PRNC - Produtividade de Raizes ñ comerciais PRNC
Peso.da.parte.aerea….CO_334.0000016 PPA
Vigor.Inicial Vigor
Vigor.da.planta.45.DAP..1-5. Vigor45D
Vigor.da.planta.6.MAP..1-5. Vigor6M
Vigor.da.planta.12.MAP..1-5. Vigor12M
colnames(DadosPhen)[c(18, 12, 13, 20)] <- c("PortePl","AltPl", "AltPlsF",
                                            "Vigor", "PPA")
RawPhenoData %>% mutate("FlowerPresence" = flower.visual.rating.0.1.CO_334.0000111,
                        "FlowerVisRate" = flowering.ability.visual.assessment.0.3.CO_334.0000233,
                     .keep = "unused") %>% melt(data = .,
                                                id.vars = c("studyYear", "locationName", "germplasmName",
                                                            "replicate", "blockNumber", "plotNumber",
                                                            "plantNumber"),
                                                variable.name = "Trait",
                                                value.name = "Value") -> PhenoData
# Rodar o script aqui dentro

Dendrogram and Shannon-Weaver Index


sessionInfo()
R version 4.1.1 (2021-08-10)
Platform: aarch64-apple-darwin20 (64-bit)
Running under: macOS Big Sur 11.6.1

Matrix products: default
LAPACK: /Library/Frameworks/R.framework/Versions/4.1-arm64/Resources/lib/libRlapack.dylib

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
 [1] reactable_0.2.3 here_1.0.1      forcats_0.5.1   stringr_1.4.0  
 [5] dplyr_1.0.7     purrr_0.3.4     readr_2.1.1     tidyr_1.1.4    
 [9] tibble_3.1.6    ggplot2_3.3.5   tidyverse_1.3.1

loaded via a namespace (and not attached):
 [1] Rcpp_1.0.7        lubridate_1.8.0   assertthat_0.2.1  rprojroot_2.0.2  
 [5] digest_0.6.29     utf8_1.2.2        reactR_0.4.4      R6_2.5.1         
 [9] cellranger_1.1.0  backports_1.4.1   reprex_2.0.1      evaluate_0.14    
[13] httr_1.4.2        pillar_1.6.4      rlang_0.4.12      readxl_1.3.1     
[17] rstudioapi_0.13   whisker_0.4       jquerylib_0.1.4   rmarkdown_2.11   
[21] htmlwidgets_1.5.4 munsell_0.5.0     broom_0.7.11      compiler_4.1.1   
[25] httpuv_1.6.5      modelr_0.1.8      xfun_0.29         pkgconfig_2.0.3  
[29] htmltools_0.5.2   tidyselect_1.1.1  workflowr_1.7.0   fansi_1.0.0      
[33] crayon_1.4.2      tzdb_0.2.0        dbplyr_2.1.1      withr_2.4.3      
[37] later_1.3.0       grid_4.1.1        jsonlite_1.7.2    gtable_0.3.0     
[41] lifecycle_1.0.1   DBI_1.1.2         git2r_0.29.0      magrittr_2.0.1   
[45] scales_1.1.1      cli_3.1.0         stringi_1.7.6     fs_1.5.2         
[49] promises_1.2.0.1  xml2_1.3.3        bslib_0.3.1       ellipsis_0.3.2   
[53] generics_0.1.1    vctrs_0.3.8       tools_4.1.1       glue_1.6.0       
[57] crosstalk_1.2.0   hms_1.1.1         fastmap_1.1.0     yaml_2.2.1       
[61] colorspace_2.0-2  rvest_1.0.2       knitr_1.37        haven_2.4.3      
[65] sass_0.4.0