Functional enrichment analysis
Maeva Techer
2025-02-11
Last updated: 2025-02-11
Checks: 5 2
Knit directory:
locust-comparative-genomics/
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Once we have shortlisted some genes of interest—whether they are obtained from top differentially expressed genes (DEGs), weighted gene co-expression network analysis (WGCNA) modules, or other comparative genomics analyses (e.g., signatures of selection, gene family expansion)—we want to determine if certain functions are enriched in our subset. For example, we hypothesize that although locusts have evolved similar traits, they may have diverged in their strategies to respond to the environment. Therefore, we expect to see DEGs involved in divergent biological processes, molecular function, and cellular components between S. gregaria, S. piceifrons and S. cancellata.
To test that, we need to look for Gene Ontology (GO) terms that can provide us a bird’s-eye of the related functions associated with our genes of interests.
1. Blast2Go file
To create the GO association file with each of our genome, we are
using the paid version of OmicsBox with the integrated
workflow Blast2Go. We details below our step-by-step with
one Schistocerca genome, but followed the same process for all
six RefSeq.
Step 1: Load Genome (fasta + GFF)
Step 2: Run Blast
We choose the More Sensitive mode of blastx from the
Diamond Blast mode which allows to align large lists of nucelotide or
protein sequences against up-to-date public sequence collections.
Diamond Blast has a very similar accuracy compared to the NCBI Blast
with a much higher throughput. All our association files were run
against the Database (NR (2024-07-11)).
2. GO term enrichment
2.1. Load necessary libraries
library(topGO)
library(dplyr)
library(ggplot2)
library(tidyr)
library(tibble)
# Define working directory and species
workDir <- "/Users/maevatecher/Documents/GitHub/locust-comparative-genomics/data"
species <- "gregaria"
# Step 1: Load DESeq2 results for the species
deg_file <- file.path(workDir, "DEG_results/Bulk_RNAseq", paste0("DESeq2_results_Head_", species, ".csv"))
deg_data <- read.csv(deg_file, stringsAsFactors = FALSE)
names(deg_data)[names(deg_data) == "X"] <- "GeneID"
# Separate DEGs into upregulated and downregulated
upregulated_genes <- subset(deg_data, padj < 0.05 & log2FoldChange > 1)$GeneID
downregulated_genes <- subset(deg_data, padj < 0.05 & log2FoldChange < -1)$GeneID
# Load the custom annotation file
custom_annot_file <- file.path(workDir, "list/GO_Annotations", paste0("blast2go_", species, "_custom.txt"))
custom_annot_df <- read.table(custom_annot_file, sep = "\t", header = TRUE, quote = "", fill = TRUE, stringsAsFactors = FALSE)
# Prepare gene-to-GO mapping for topGO
colnames(custom_annot_df) <- c("GeneID", "Description", "GO_Extended")
#custom_annot_df <- custom_annot_df %>%
# separate(GO_Extended, into = c("Category", "GO_ID", "GO_Term"), sep = " ", extra = "merge") %>%
# mutate(Category = substr(Category, 1, 1))
library(data.table)
# Convert to data.table (if not already)
setDT(custom_annot_df)
# Split `GO_Extended` column efficiently
custom_annot_df[, c("Category", "GO_ID", "GO_Term") := tstrsplit(GO_Extended, " ", fixed = TRUE, keep = 1:3)]
# Extract first letter of `Category`
custom_annot_df[, Category := substr(Category, 1, 1)]
gene2GO <- custom_annot_df %>%
group_by(GeneID) %>%
summarize(GOterms = list(unique(GO_ID))) %>%
deframe()
# Function to run topGO analysis by ontology
run_topGO <- function(ontology, gene_set, gene2GO) {
all_genes <- factor(as.integer(names(gene2GO) %in% gene_set), levels = c(0, 1))
names(all_genes) <- names(gene2GO)
GOdata <- new("topGOdata", ontology = ontology, allGenes = all_genes, annot = annFUN.gene2GO, gene2GO = gene2GO)
resultFisher <- runTest(GOdata, algorithm = "classic", statistic = "fisher")
GenTable(GOdata, classicFisher = resultFisher, orderBy = "classicFisher", topNodes = 10)
}
# Run topGO for each ontology category and regulation type
allRes_up_BP <- run_topGO("BP", upregulated_genes, gene2GO) %>% mutate(Regulation = "Upregulated", ontology = "BP")
allRes_up_MF <- run_topGO("MF", upregulated_genes, gene2GO) %>% mutate(Regulation = "Upregulated", ontology = "MF")
allRes_up_CC <- run_topGO("CC", upregulated_genes, gene2GO) %>% mutate(Regulation = "Upregulated", ontology = "CC")
allRes_down_BP <- run_topGO("BP", downregulated_genes, gene2GO) %>% mutate(Regulation = "Downregulated", ontology = "BP")
allRes_down_MF <- run_topGO("MF", downregulated_genes, gene2GO) %>% mutate(Regulation = "Downregulated", ontology = "MF")
allRes_down_CC <- run_topGO("CC", downregulated_genes, gene2GO) %>% mutate(Regulation = "Downregulated", ontology = "CC")
# Combine all results with ontology labels
allRes <- bind_rows(
allRes_up_BP, allRes_up_MF, allRes_up_CC,
allRes_down_BP, allRes_down_MF, allRes_down_CC
)
# Check if ontology is retained
head(allRes) GO.ID Term Annotated Significant Expected
1 GO:0055085 transmembrane transport 649 29 12.33
2 GO:0006810 transport 987 36 18.75
3 GO:0051234 establishment of localization 1002 36 19.03
4 GO:0051179 localization 1012 36 19.22
5 GO:0019310 inositol catabolic process 6 3 0.11
6 GO:0046164 alcohol catabolic process 7 3 0.13
classicFisher Regulation ontology
1 5.6e-06 Upregulated BP
2 3.1e-05 Upregulated BP
3 4.4e-05 Upregulated BP
4 5.5e-05 Upregulated BP
5 0.00013 Upregulated BP
6 0.00022 Upregulated BP# Visualization with ggplot2
allRes$classicFisher <- as.numeric(as.character(allRes$classicFisher))
allRes$FoldEnrichment <- allRes$Significant / allRes$Expected# Plot with ggplot2 using facet_wrap by ontology
ggplot(allRes, aes(x = reorder(Term, -log10(classicFisher)), y = -log10(classicFisher), size = Significant, color = FoldEnrichment)) +
geom_point() +
facet_wrap(~ ontology, scales = "free_y") +
coord_flip() +
labs(
x = "GO Term",
y = "-log10(p-value)",
size = "Gene Count",
color = "Fold Enrichment",
title = "Top 10 Enriched GO Terms by Ontology",
subtitle = "Head transcriptomes S. gregaria"
) +
theme_minimal() +
theme(
plot.title = element_text(size = 16, face = "bold"),
plot.subtitle = element_text(size = 12, face = "bold.italic"),
axis.text.y = element_text(size = 8, face = "bold.italic", color = "black")
) +
scale_size_continuous(range = c(3, 10)) +
scale_color_viridis_c(option = "D")
# Plotting code up and downregulated
ggplot(allRes, aes(x = reorder(Term, -log10(classicFisher)), y = -log10(classicFisher), size = Significant, color = Regulation)) +
geom_point() +
facet_wrap(~ ontology, scales = "free_y") +
coord_flip() +
labs(
x = "GO Term",
y = "-log10(p-value)",
size = "Gene Count",
color = "Regulation",
title = "Top 10 Enriched GO Terms by Ontology",
subtitle = "Head transcriptomes S. gregaria"
) +
theme_minimal() +
theme(
plot.title = element_text(size = 16, face = "bold"),
plot.subtitle = element_text(size = 12, face = "italic"),
axis.text.y = element_text(size = 8, face = "bold", color = "black")
) +
scale_size_continuous(range = c(3, 10)) +
scale_color_manual(values = c("Upregulated" = "red", "Downregulated" = "blue"))
# Bar Plot for top GO terms per ontology
ggplot(allRes, aes(x = FoldEnrichment, y = reorder(Term, FoldEnrichment), color = -log10(classicFisher), size = Significant)) +
geom_point() +
facet_wrap(~ ontology, scales = "free_y") +
labs(
x = "Fold Enrichment",
y = "GO Term",
color = "-log10(p-value)",
size = "Gene Count",
title = "GO Term Enrichment: Fold Enrichment vs p-value"
) +
theme_minimal() +
theme(
plot.title = element_text(size = 16, face = "bold"),
plot.subtitle = element_text(size = 12, face = "bold.italic"),
axis.text.y = element_text(size = 8, face = "bold", color = "black")
) +
scale_fill_viridis_c(option = "C")
library(pheatmap)
# Aggregate data to ensure unique combinations of Term and ontology
# Keep the row with the smallest classicFisher value for each Term and ontology pair
heatmap_data <- allRes %>%
dplyr::group_by(Term, ontology) %>%
dplyr::slice_min(order_by = classicFisher, n = 1) %>%
dplyr::ungroup() %>%
dplyr::select(Term, ontology, classicFisher, Regulation) %>%
tidyr::spread(ontology, classicFisher) %>%
tibble::column_to_rownames("Term")
# Verify heatmap data
str(heatmap_data)'data.frame': 57 obs. of 4 variables:
$ Regulation: chr "Upregulated" "Downregulated" "Downregulated" "Downregulated" ...
$ BP : num 0.00022 0.0004 0.00023 NA NA 0.00023 0.0005 NA NA NA ...
$ CC : num NA NA NA NA 0.244 ...
$ MF : num NA NA NA 4.3e-04 NA NA NA 4.1e-06 NA NA ...# Ensure Regulation exists
if (!"Regulation" %in% colnames(heatmap_data)) {
stop("Error: 'Regulation' column is missing from heatmap_data!")
}
# Create annotation data frame
annotation <- data.frame(Regulation = heatmap_data$Regulation)
rownames(annotation) <- rownames(heatmap_data)
# Ensure no NA values
annotation <- na.omit(annotation)
# Define annotation colors
annotation_colors <- list(
Regulation = c("Upregulated" = "red", "Downregulated" = "blue")
)
# Ensure annotation_colors matches annotation values
if (!all(unique(annotation$Regulation) %in% names(annotation_colors$Regulation))) {
stop("Error: annotation_colors does not match all values in annotation$Regulation")
}
# Create heatmap matrix
heatmap_matrix <- as.matrix(-log10(heatmap_data %>%
dplyr::select(-Regulation)))
# Replace NA values
heatmap_matrix[is.na(heatmap_matrix)] <- -log10(1)
# Plot heatmap
pheatmap(
heatmap_matrix,
cluster_rows = TRUE,
cluster_cols = FALSE,
color = colorRampPalette(c("white", "darkblue"))(50),
annotation_row = annotation,
main = "GO Term Enrichment Heatmap by Ontology",
annotation_colors = annotation_colors
)
# Define working directory and species list
workDir <- "/Users/maevatecher/Documents/GitHub/locust-comparative-genomics/data"
species_list <- c("gregaria", "piceifrons", "cancellata", "americana", "cubense", "nitens")
# Function to run the GO analysis for a given species
run_GO_analysis_for_species <- function(species) {
# Load DESeq2 results for the species
deg_file <- file.path(workDir, "DEG_results/Bulk_RNAseq", paste0("DESeq2_results_Head_", species, ".csv"))
deg_data <- read.csv(deg_file, stringsAsFactors = FALSE)
names(deg_data)[names(deg_data) == "X"] <- "GeneID"
# Separate DEGs into upregulated and downregulated
upregulated_genes <- subset(deg_data, padj < 0.05 & log2FoldChange > 1)$GeneID
downregulated_genes <- subset(deg_data, padj < 0.05 & log2FoldChange < -1)$GeneID
# Load the custom annotation file
custom_annot_file <- file.path(workDir, "list/GO_Annotations", paste0("blast2go_", species, "_custom.txt"))
custom_annot_df <- read.table(custom_annot_file, sep = "\t", header = TRUE, quote = "", fill = TRUE, stringsAsFactors = FALSE)
# Prepare gene-to-GO mapping for topGO
colnames(custom_annot_df) <- c("GeneID", "Description", "GO_Extended")
library(data.table)
# Convert to data.table (if not already)
setDT(custom_annot_df)
# Split `GO_Extended` column efficiently
custom_annot_df[, c("Category", "GO_ID", "GO_Term") := tstrsplit(GO_Extended, " ", fixed = TRUE, keep = 1:3)]
# Extract first letter of `Category`
custom_annot_df[, Category := substr(Category, 1, 1)]
gene2GO <- custom_annot_df %>%
group_by(GeneID) %>%
summarize(GOterms = list(unique(GO_ID))) %>%
deframe()
# Function to run topGO analysis by ontology
run_topGO <- function(ontology, gene_set, gene2GO) {
all_genes <- factor(as.integer(names(gene2GO) %in% gene_set), levels = c(0, 1))
names(all_genes) <- names(gene2GO)
GOdata <- new("topGOdata", ontology = ontology, allGenes = all_genes, annot = annFUN.gene2GO, gene2GO = gene2GO)
resultFisher <- runTest(GOdata, algorithm = "classic", statistic = "fisher")
GenTable(GOdata, classicFisher = resultFisher, orderBy = "classicFisher", topNodes = 10)
}
# Run topGO for each ontology category and regulation type
allRes_up_BP <- run_topGO("BP", upregulated_genes, gene2GO) %>% mutate(Regulation = "Upregulated", ontology = "BP")
allRes_up_MF <- run_topGO("MF", upregulated_genes, gene2GO) %>% mutate(Regulation = "Upregulated", ontology = "MF")
allRes_up_CC <- run_topGO("CC", upregulated_genes, gene2GO) %>% mutate(Regulation = "Upregulated", ontology = "CC")
allRes_down_BP <- run_topGO("BP", downregulated_genes, gene2GO) %>% mutate(Regulation = "Downregulated", ontology = "BP")
allRes_down_MF <- run_topGO("MF", downregulated_genes, gene2GO) %>% mutate(Regulation = "Downregulated", ontology = "MF")
allRes_down_CC <- run_topGO("CC", downregulated_genes, gene2GO) %>% mutate(Regulation = "Downregulated", ontology = "CC")
# Combine all results with ontology labels
allRes <- bind_rows(
allRes_up_BP, allRes_up_MF, allRes_up_CC,
allRes_down_BP, allRes_down_MF, allRes_down_CC
)
# Calculate FoldEnrichment and convert p-values
allRes$classicFisher <- as.numeric(as.character(allRes$classicFisher))
allRes$FoldEnrichment <- allRes$Significant / allRes$Expected
# Export results for this species
output_file <- file.path(workDir, "DEG_results/Bulk_RNAseq", paste0("GO10_enrichment_Head_", species, "_custom.csv"))
write.csv(allRes, output_file, row.names = FALSE)
return(allRes)
}
# Name each element in species_list
names(species_list) <- species_list
# Run the analysis for each species
results_list <- lapply(species_list, run_GO_analysis_for_species)
# Combine all results into a single table if desired
combined_results <- bind_rows(results_list, .id = "Species")# Define working directory and species list
workDir <- "/Users/maevatecher/Documents/GitHub/locust-comparative-genomics/data"
species_list <- c("gregaria", "piceifrons", "cancellata", "americana", "cubense", "nitens")
# Function to run the GO analysis for a given species
run_GO_analysis_for_species <- function(species) {
# Load DESeq2 results for the species
deg_file <- file.path(workDir, "DEG_results/Bulk_RNAseq", paste0("DESeq2_results_Thorax_", species, ".csv"))
deg_data <- read.csv(deg_file, stringsAsFactors = FALSE)
names(deg_data)[names(deg_data) == "X"] <- "GeneID"
# Separate DEGs into upregulated and downregulated
upregulated_genes <- subset(deg_data, padj < 0.05 & log2FoldChange > 1)$GeneID
downregulated_genes <- subset(deg_data, padj < 0.05 & log2FoldChange < -1)$GeneID
# Load the custom annotation file
custom_annot_file <- file.path(workDir, "list/GO_Annotations", paste0("blast2go_", species, "_custom.txt"))
custom_annot_df <- read.table(custom_annot_file, sep = "\t", header = TRUE, quote = "", fill = TRUE, stringsAsFactors = FALSE)
# Prepare gene-to-GO mapping for topGO
colnames(custom_annot_df) <- c("GeneID", "Description", "GO_Extended")
library(data.table)
# Convert to data.table (if not already)
setDT(custom_annot_df)
# Split `GO_Extended` column efficiently
custom_annot_df[, c("Category", "GO_ID", "GO_Term") := tstrsplit(GO_Extended, " ", fixed = TRUE, keep = 1:3)]
# Extract first letter of `Category`
custom_annot_df[, Category := substr(Category, 1, 1)]
gene2GO <- custom_annot_df %>%
group_by(GeneID) %>%
summarize(GOterms = list(unique(GO_ID))) %>%
deframe()
# Function to run topGO analysis by ontology
run_topGO <- function(ontology, gene_set, gene2GO) {
all_genes <- factor(as.integer(names(gene2GO) %in% gene_set), levels = c(0, 1))
names(all_genes) <- names(gene2GO)
GOdata <- new("topGOdata", ontology = ontology, allGenes = all_genes, annot = annFUN.gene2GO, gene2GO = gene2GO)
resultFisher <- runTest(GOdata, algorithm = "classic", statistic = "fisher")
GenTable(GOdata, classicFisher = resultFisher, orderBy = "classicFisher", topNodes = 10)
}
# Run topGO for each ontology category and regulation type
allRes_up_BP <- run_topGO("BP", upregulated_genes, gene2GO) %>% mutate(Regulation = "Upregulated", ontology = "BP")
allRes_up_MF <- run_topGO("MF", upregulated_genes, gene2GO) %>% mutate(Regulation = "Upregulated", ontology = "MF")
allRes_up_CC <- run_topGO("CC", upregulated_genes, gene2GO) %>% mutate(Regulation = "Upregulated", ontology = "CC")
allRes_down_BP <- run_topGO("BP", downregulated_genes, gene2GO) %>% mutate(Regulation = "Downregulated", ontology = "BP")
allRes_down_MF <- run_topGO("MF", downregulated_genes, gene2GO) %>% mutate(Regulation = "Downregulated", ontology = "MF")
allRes_down_CC <- run_topGO("CC", downregulated_genes, gene2GO) %>% mutate(Regulation = "Downregulated", ontology = "CC")
# Combine all results with ontology labels
allRes <- bind_rows(
allRes_up_BP, allRes_up_MF, allRes_up_CC,
allRes_down_BP, allRes_down_MF, allRes_down_CC
)
# Calculate FoldEnrichment and convert p-values
allRes$classicFisher <- as.numeric(as.character(allRes$classicFisher))
allRes$FoldEnrichment <- allRes$Significant / allRes$Expected
# Export results for this species
output_file <- file.path(workDir, "DEG_results/Bulk_RNAseq", paste0("GO10_enrichment_Thorax_", species, "_custom.csv"))
write.csv(allRes, output_file, row.names = FALSE)
return(allRes)
}
# Name each element in species_list
names(species_list) <- species_list
# Run the analysis for each species
results_list <- lapply(species_list, run_GO_analysis_for_species)
# Combine all results into a single table if desired
combined_results <- bind_rows(results_list, .id = "Species")
sessionInfo()R version 4.4.1 (2024-06-14)
Platform: aarch64-apple-darwin20
Running under: macOS 15.3
Matrix products: default
BLAS: /Library/Frameworks/R.framework/Versions/4.4-arm64/Resources/lib/libRblas.0.dylib
LAPACK: /Library/Frameworks/R.framework/Versions/4.4-arm64/Resources/lib/libRlapack.dylib; LAPACK version 3.12.0
locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
time zone: Asia/Tokyo
tzcode source: internal
attached base packages:
[1] stats4 stats graphics grDevices utils datasets methods
[8] base
other attached packages:
[1] pheatmap_1.0.12 data.table_1.16.4 tibble_3.2.1
[4] tidyr_1.3.1 ggplot2_3.5.1 dplyr_1.1.4
[7] topGO_2.56.0 SparseM_1.84-2 GO.db_3.19.1
[10] AnnotationDbi_1.66.0 IRanges_2.38.1 S4Vectors_0.42.1
[13] Biobase_2.64.0 graph_1.82.0 BiocGenerics_0.50.0
loaded via a namespace (and not attached):
[1] KEGGREST_1.44.1 gtable_0.3.6 xfun_0.50
[4] bslib_0.9.0 lattice_0.22-6 vctrs_0.6.5
[7] tools_4.4.1 generics_0.1.3 RSQLite_2.3.9
[10] blob_1.2.4 pkgconfig_2.0.3 RColorBrewer_1.1-3
[13] lifecycle_1.0.4 GenomeInfoDbData_1.2.12 farver_2.1.2
[16] compiler_4.4.1 stringr_1.5.1 git2r_0.35.0
[19] Biostrings_2.72.1 munsell_0.5.1 httpuv_1.6.15
[22] GenomeInfoDb_1.40.1 htmltools_0.5.8.1 sass_0.4.9
[25] yaml_2.3.10 later_1.4.1 pillar_1.10.1
[28] crayon_1.5.3 jquerylib_0.1.4 whisker_0.4.1
[31] cachem_1.1.0 tidyselect_1.2.1 digest_0.6.37
[34] stringi_1.8.4 purrr_1.0.4 labeling_0.4.3
[37] rprojroot_2.0.4 fastmap_1.2.0 grid_4.4.1
[40] colorspace_2.1-1 cli_3.6.3 magrittr_2.0.3
[43] withr_3.0.2 scales_1.3.0 UCSC.utils_1.0.0
[46] promises_1.3.2 bit64_4.6.0-1 rmarkdown_2.29
[49] XVector_0.44.0 httr_1.4.7 matrixStats_1.5.0
[52] bit_4.5.0.1 workflowr_1.7.1 png_0.1-8
[55] memoise_2.0.1 evaluate_1.0.3 knitr_1.49
[58] viridisLite_0.4.2 rlang_1.1.5 Rcpp_1.0.14
[61] glue_1.8.0 DBI_1.2.3 rstudioapi_0.17.1
[64] jsonlite_1.8.9 R6_2.5.1 fs_1.6.5
[67] zlibbioc_1.50.0