Last updated: 2024-05-16
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Once we have shortlisted some genes of interest—whether they are obtained from top differentially expressed genes (DEGs), weighted gene co-expression network analysis (WGCNA) modules, or other comparative genomics analyses (e.g., signatures of selection, gene family expansion)—we want to determine if certain functions are enriched in our subset. For example, we hypothesize that although locusts have evolved similar traits, they may have diverged in their strategies to respond to the environment. Therefore, we expect to see DEGs involved in divergent biological processes, molecular function, and cellular components between S. gregaria, S. piceifrons and S. cancellata.
To test that, we need to look for Gene Ontology (GO) terms that can provide us a bird’s-eye of the related functions associated with our genes of interests.
Blast2Go file
GO term enrichment
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