Last updated: 2024-11-11

Checks: 1 1

Knit directory: test-3/

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Repository version: 7258162

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    Modified:   analysis/about.Rmd
    Modified:   analysis/index.Rmd

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These are the previous versions of the repository in which changes were made to the R Markdown (analysis/index.Rmd) and HTML (docs/index.html) files. If you’ve configured a remote Git repository (see ?wflow_git_remote), click on the hyperlinks in the table below to view the files as they were in that past version.

File Version Author Date Message
Rmd 7258162 Paloma 2024-11-09 improved explanations of experiment
html 7258162 Paloma 2024-11-09 improved explanations of experiment
Rmd e435bbc Paloma 2024-10-25 update result plots_non spiked
html e435bbc Paloma 2024-10-25 update result plots_non spiked
html 91fbafe Paloma 2024-10-17 Build site.
Rmd f42c096 Paloma 2024-10-17 index
html f42c096 Paloma 2024-10-17 index
Rmd 14c1638 Paloma 2024-10-17 update links again
html 14c1638 Paloma 2024-10-17 update links again
Rmd e8567d9 Paloma 2024-10-17 update links
html e8567d9 Paloma 2024-10-17 update links
html e56ef2f Paloma 2024-10-17 Build site.
html 8e75be7 Paloma 2024-10-17 Update index.html
html f28b5e3 Paloma 2024-10-17 Build site.
html 4ee17fb Paloma 2024-10-17 Build site.
Rmd 70a7581 Paloma 2024-10-17 about page and index
html 2ed0880 Paloma 2024-10-17 Build site.
Rmd 4594242 Paloma 2024-10-17 about page
html 35b1a01 GitHub 2024-10-17 Update index.html
html 62a7e39 GitHub 2024-10-17 Update index.html
html 4e7632d Paloma 2024-10-17 Update index.html
html e4cf6f3 Paloma 2024-10-16 initial files
Rmd fd1d59b Paloma 2024-10-16 Start workflowr project.

Evaluation of hair cortisol ELISA protocol proposed by Nist et al. 2020

This protocol includes additional steps that would make possible to quantify low-mass hair samples (i.e. less than 20 mg). Specifically, that study quantifies cortisol in hair samples form neonates.

We tested this protocol by running an ELISA plate with 40 samples from one adult individual. In order to find optimal parameters, we tested different mass, dilution, and addition (or not) of a spike.

Our results indicate that the method proposed by Nist et al. 2020 does not give us reliable results, and does not allow us to quantify cortisol from low-mass hair from adults.

We found that a dilution of 250 uL, not adding a spike, and using between 20 to 35 mg of hair provides the most consistent results.

You can click this link to read a more detailed description of this experiment.