Last updated: 2020-01-20
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Knit directory: 20170327_Psen2S4Ter_RNASeq/
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| Rmd | acb4f8a | Steve Ped | 2020-01-19 | Start workflowr project. |
Analysis of RNASeq data from the 3way comparison of WT zebrafish samples with Heterozygous mutants for psen2S4Ter and Homozygous mutants for psen2S4Ter. This mutant is expected to be a premature termination of the psen2 gene, but instead an alternate downstream start site resulted in a near full-length transcript with significant similarity to a conventional Early Onset Familial Alzhemier’s Disease mutation.
RNAseq was performed on total RNA with \(n=4\) samples in each group. Sequencing was performed by the sequencing facility at the Centre for Cancer Biology in Adelaide, using a NextSeq. Reads were provided in as paired-end, 150bp reads.