Nuclear fraction and nascent transcription

Create transcription phenotype

4su data

FourSU=read.table(file = "../data/fourSU//tr_decay_table_norm.txt", header=T, stringsAsFactors = F) %>%  dplyr::select(gene_id,contains("4su_30"))


FourSU_geneNames=FourSU %>% inner_join(geneNames, by="gene_id") %>% dplyr::select(GeneName, contains("4su_30"))

FourgeneNames_long=melt(FourSU_geneNames,id.vars = "GeneName", value.name = "FourSU", variable.name = "FourSU_ind") %>% separate(FourSU_ind, into=c("type","time", "1400", "MAf", "Individual"), sep="_") %>% dplyr::select(GeneName, Individual, FourSU) 

FourSU_geneMean=FourgeneNames_long %>% group_by(GeneName) %>%summarise(Mean_4su=mean(FourSU))

rna seq

RNA=read.table(file = "../data/fourSU/tr_decay_table_norm.txt", header=T, stringsAsFactors = F) %>%  dplyr::select(gene_id,contains("RNAseq_14000"))


RNA_geneNames=RNA %>% inner_join(geneNames, by="gene_id") %>% dplyr::select(GeneName, contains("RNA"))

RNAgeneNames_long=melt(RNA_geneNames,id.vars = "GeneName", value.name = "RNA", variable.name = "RNA_ind") %>%   separate(RNA_ind, into=c("type", "1400", "MAf", "Individual"), sep="_") %>% dplyr::select(GeneName, Individual, RNA) 

RNA_geneMean=RNAgeneNames_long %>% group_by(GeneName) %>%summarise(Mean_RNA=mean(RNA))

Make transcription phenotype

Transcription=FourSU_geneMean %>% inner_join(RNA_geneMean, by="GeneName") %>% mutate(Transcription=Mean_4su/(Mean_4su + Mean_RNA)) %>% dplyr::select(GeneName, Transcription) %>% dplyr::rename("gene"=GeneName)

Transcription2=FourSU_geneMean %>% inner_join(RNA_geneMean, by="GeneName") %>% mutate(Transcription=Mean_4su/Mean_RNA) %>% dplyr::select(GeneName, Transcription) %>% dplyr::rename("gene"=GeneName)

APA phenotype

5 perc apa

peaknumlist=read.table("../data/peaks_5perc/APApeak_Peaks_GeneLocAnno.5perc.bed", stringsAsFactors = F, header=F, col.names = c("chr", "start","end", "id", "score", "strand"))  %>% separate(id, into=c("peaknum", "geneid"), sep=":") %>% mutate(peakid=paste("peak", peaknum,sep=""))

Nuclear apa

NucAPA=read.table("../data/peakCoverage/APAPeaks.ALLChrom.Filtered.Named.GeneLocAnnoPARSED.Nuclear.Quant.Fixed.fc", stringsAsFactors = F, header = T) %>% dplyr::select(-Chr, -Start, -End, -Strand, -Length) %>% separate(Geneid, into=c("peakid","chrom", "start", "end", "strand", "geneID"),sep=":") %>% semi_join(peaknumlist, by="peakid") %>% separate(geneID, into=c("gene", "loc"), sep="_") %>% dplyr::select(-chrom , -start, -end, -strand, -loc)

NucApaMelt=melt(NucAPA, id.vars =c( "peakid", "gene"), value.name="count", variable.name="Ind") %>% separate(Ind, into=c('Individual', 'fraction') ,sep="_")%>% dplyr::select(peakid, gene, Individual, count)


NucAPA_bygene= NucApaMelt %>% group_by(gene,Individual) %>% summarise(NuclearSum=sum(count))

total apa

TotAPA=read.table("../data/peakCoverage/APAPeaks.ALLChrom.Filtered.Named.GeneLocAnnoPARSED.Total.Quant.Fixed.fc", stringsAsFactors = F, header = T) %>% dplyr::select(-Chr, -Start, -End, -Strand, -Length) %>% separate(Geneid, into=c("peakid","chrom", "start", "end", "strand", "geneID"),sep=":") %>% semi_join(peaknumlist, by="peakid") %>% separate(geneID, into=c("gene", "loc"), sep="_") %>% dplyr::select(-chrom , -start, -end, -strand, -loc)

TotApaMelt=melt(TotAPA, id.vars =c( "peakid", "gene"), value.name="count", variable.name="Ind") %>% separate(Ind, into=c('Individual', 'fraction') ,sep="_")%>% dplyr::select(peakid, gene, Individual, count)


TotAPA_bygene= TotApaMelt %>% group_by(gene,Individual) %>% summarise(TotalSum=sum(count))

Sum together:

ApaBothFrac=TotAPA_bygene %>% inner_join(NucAPA_bygene, by=c("gene", "Individual"))

ApaBothFrac_melt=melt(ApaBothFrac, id.vars=c("gene", "Individual"),value.name="APA_val" ) %>% mutate(fraction=ifelse(variable=="TotalSum", "total", "nuclear"), line=paste("NA", substring(Individual, 2), sep="")) %>% dplyr::select(gene, fraction, line, APA_val)

Normalize with meta data info:

metadata=read.table("../data/MetaDataSequencing.txt", header = T,stringsAsFactors = F) %>% dplyr::select(line, fraction, Mapped_noMP)

metadata$line= as.character(metadata$line)

ApaBothFracStand=ApaBothFrac_melt %>% full_join(metadata, by=c("line", "fraction")) %>% mutate(StandApa=APA_val/Mapped_noMP)

ApaBothFracStand_geneMean=ApaBothFracStand %>% group_by(fraction, gene) %>% summarise(meanAPA=mean(StandApa, na.rm=T))

ApaBothFracStand_geneMean_spread= spread(ApaBothFracStand_geneMean,fraction,meanAPA ) %>% mutate(APAVal=nuclear/(total+ nuclear)) 

Join data and plot

Density function:

get_density <- function(x, y, ...) {
  dens <- MASS::kde2d(x, y, ...)
  ix <- findInterval(x, dens$x)
  iy <- findInterval(y, dens$y)
  ii <- cbind(ix, iy)
  return(dens$z[ii])
}

set.seed(1)
dat <- data.frame(
  x = c(
    rnorm(1e4, mean = 0, sd = 0.1),
    rnorm(1e3, mean = 0, sd = 0.1)
  ),
  y = c(
    rnorm(1e4, mean = 0, sd = 0.1),
    rnorm(1e3, mean = 0.1, sd = 0.2)
  )
)

Joing apa and transcription

APAandTranscrption= Transcription %>% inner_join(ApaBothFracStand_geneMean_spread, by="gene")
APAandTranscrption$density <- get_density(APAandTranscrption$APAVal, APAandTranscrption$Transcription, n = 100)



summary(lm(data=APAandTranscrption, APAVal~Transcription))

Call:
lm(formula = APAVal ~ Transcription, data = APAandTranscrption)

Residuals:
     Min       1Q   Median       3Q      Max 
-0.37416 -0.09802  0.01235  0.10753  0.38048 

Coefficients:
              Estimate Std. Error t value Pr(>|t|)    
(Intercept)   0.393873   0.007265   54.22   <2e-16 ***
Transcription 0.285418   0.013551   21.06   <2e-16 ***
---
Signif. codes:  0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1

Residual standard error: 0.1338 on 7885 degrees of freedom
Multiple R-squared:  0.05326,   Adjusted R-squared:  0.05314 
F-statistic: 443.6 on 1 and 7885 DF,  p-value: < 2.2e-16

Plot:

ggplot(APAandTranscrption, aes(x=Transcription, y=APAVal))+ geom_point(aes(color=density)) + geom_smooth(method = "lm") + labs(x="4su/4su+RNA", y="Nuclear/Nuclear+Total", title="Relationship between APA fraction and transcription") + scale_color_viridis()

Past versions of unnamed-chunk-13-1.png

Split Nuclear by intronic

NucAPAIntron=read.table("../data/peakCoverage/APAPeaks.ALLChrom.Filtered.Named.GeneLocAnnoPARSED.Nuclear.Quant.Fixed.fc", stringsAsFactors = F, header = T) %>% dplyr::select(-Chr, -Start, -End, -Strand, -Length) %>% separate(Geneid, into=c("peakid","chrom", "start", "end", "strand", "geneID"),sep=":") %>% semi_join(peaknumlist, by="peakid") %>% separate(geneID, into=c("gene", "loc"), sep="_") %>% filter(loc=="intron")%>% dplyr::select(-chrom , -start, -end, -strand, -loc)

NucApaIntronMelt=melt(NucAPAIntron, id.vars =c( "peakid", "gene"), value.name="count", variable.name="Ind") %>% separate(Ind, into=c('Individual', 'fraction') ,sep="_")%>% dplyr::select(peakid, gene, Individual, count)


NucAPAIntron_bygene= NucApaIntronMelt %>% group_by(gene,Individual) %>% summarise(NuclearIntronSum=sum(count))

TotUTRAPA=read.table("../data/peakCoverage/APAPeaks.ALLChrom.Filtered.Named.GeneLocAnnoPARSED.Total.Quant.Fixed.fc", stringsAsFactors = F, header = T) %>% dplyr::select(-Chr, -Start, -End, -Strand, -Length) %>% separate(Geneid, into=c("peakid","chrom", "start", "end", "strand", "geneID"),sep=":") %>% semi_join(peaknumlist, by="peakid") %>% separate(geneID, into=c("gene", "loc"), sep="_") %>%filter(loc=="utr3") %>%  dplyr::select(-chrom , -start, -end, -strand, -loc)

TotApaUTRMelt=melt(TotUTRAPA, id.vars =c( "peakid", "gene"), value.name="count", variable.name="Ind") %>% separate(Ind, into=c('Individual', 'fraction') ,sep="_")%>% dplyr::select(peakid, gene, Individual, count)


TotAPAUTR_bygene= TotApaUTRMelt %>% group_by(gene,Individual) %>% summarise(TotalUTRSum=sum(count))

ApaBothFracLoc=TotAPAUTR_bygene %>% inner_join(NucAPAIntron_bygene, by=c("gene", "Individual"))

ApaBothFracLoc_melt=melt(ApaBothFracLoc, id.vars=c("gene", "Individual"),value.name="APA_val" ) %>% mutate(fraction=ifelse(variable=="TotalUTRSum", "total", "nuclear"), line=paste("NA", substring(Individual, 2), sep="")) %>% dplyr::select(gene, fraction, line, APA_val)


ApaBothFracLocStand=ApaBothFracLoc_melt %>% full_join(metadata, by=c("line", "fraction")) %>% mutate(StandApa=APA_val/Mapped_noMP)

ApaBothFracLocStand_geneMean=ApaBothFracLocStand %>% group_by(fraction, gene) %>% summarise(meanAPA=mean(StandApa, na.rm=T))

ApaBothFracLocStand_geneMean_spread= spread(ApaBothFracLocStand_geneMean,fraction,meanAPA ) %>% mutate(APAValLoc=nuclear/(total+nuclear)) 

ApaBothFracLocStand_geneMean_spread2= spread(ApaBothFracLocStand_geneMean,fraction,meanAPA ) %>% mutate(APAValLoc=nuclear/total) 

APAlocationandTranscrption= Transcription %>% inner_join(ApaBothFracLocStand_geneMean_spread, by="gene")
APAlocationandTranscrption$density <- get_density(APAlocationandTranscrption$APAValLoc, APAlocationandTranscrption$Transcription, n = 100)

ggplot(APAlocationandTranscrption, aes(x=Transcription, y=APAValLoc))+ geom_point(aes(color=density)) + geom_smooth(method = "lm") + labs(x="4su/4su+RNA", y="NuclearIntron/TotalUTR + IntronNuclear", title="Relationship between APA fraction and transcription") + scale_color_viridis()

summary(lm(data=APAlocationandTranscrption, APAValLoc~Transcription))

Call:
lm(formula = APAValLoc ~ Transcription, data = APAlocationandTranscrption)

Residuals:
     Min       1Q   Median       3Q      Max 
-0.40940 -0.19655 -0.07216  0.15348  0.80483 

Coefficients:
              Estimate Std. Error t value Pr(>|t|)    
(Intercept)    0.02328    0.01768   1.317    0.188    
Transcription  0.47752    0.03232  14.777   <2e-16 ***
---
Signif. codes:  0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1

Residual standard error: 0.2455 on 5491 degrees of freedom
Multiple R-squared:  0.03825,   Adjusted R-squared:  0.03807 
F-statistic: 218.4 on 1 and 5491 DF,  p-value: < 2.2e-16

APAlocationandTranscrption2= Transcription2 %>% inner_join(ApaBothFracLocStand_geneMean_spread2, by="gene")
APAlocationandTranscrption2$density <- get_density(APAlocationandTranscrption2$APAValLoc, APAlocationandTranscrption2$Transcription, n = 100)
summary(lm(data=APAlocationandTranscrption2, log10(APAValLoc)~log10(Transcription)))

Call:
lm(formula = log10(APAValLoc) ~ log10(Transcription), data = APAlocationandTranscrption2)

Residuals:
    Min      1Q  Median      3Q     Max 
-3.2920 -0.4924  0.0373  0.5100  3.3917 

Coefficients:
                     Estimate Std. Error t value Pr(>|t|)    
(Intercept)          -0.69768    0.01097  -63.59   <2e-16 ***
log10(Transcription)  0.90749    0.05474   16.58   <2e-16 ***
---
Signif. codes:  0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1

Residual standard error: 0.7643 on 5491 degrees of freedom
Multiple R-squared:  0.04766,   Adjusted R-squared:  0.04749 
F-statistic: 274.8 on 1 and 5491 DF,  p-value: < 2.2e-16

ggplot(APAlocationandTranscrption2, aes(x=log10(Transcription), y=log10(APAValLoc)))+ geom_point(aes(color=density)) + geom_smooth(method = "lm") + labs(x="log10(4su/RNA)", y="log10(NuclearIntron/TotalUTR)", title="Relationship between APA fraction and transcription") + scale_color_viridis()

Compare nuclear and total UTR

NucAPAUTR=read.table("../data/peakCoverage/APAPeaks.ALLChrom.Filtered.Named.GeneLocAnnoPARSED.Nuclear.Quant.Fixed.fc", stringsAsFactors = F, header = T) %>% dplyr::select(-Chr, -Start, -End, -Strand, -Length) %>% separate(Geneid, into=c("peakid","chrom", "start", "end", "strand", "geneID"),sep=":") %>% semi_join(peaknumlist, by="peakid") %>% separate(geneID, into=c("gene", "loc"), sep="_") %>% filter(loc=="utr3")%>% dplyr::select(-chrom , -start, -end, -strand, -loc)

NucAPAUTRMelt=melt(NucAPAUTR, id.vars =c( "peakid", "gene"), value.name="count", variable.name="Ind") %>% separate(Ind, into=c('Individual', 'fraction') ,sep="_")%>% dplyr::select(peakid, gene, Individual, count)


NucAPAUTR_bygene= NucAPAUTRMelt %>% group_by(gene,Individual) %>% summarise(NuclearUTRSum=sum(count))

ApaBothFracUTR=TotAPAUTR_bygene %>% inner_join(NucAPAUTR_bygene, by=c("gene", "Individual"))

ApaBothFracUTR_melt=melt(ApaBothFracUTR, id.vars=c("gene", "Individual"),value.name="APA_val" ) %>% mutate(fraction=ifelse(variable=="TotalUTRSum", "total", "nuclear"), line=paste("NA", substring(Individual, 2), sep="")) %>% dplyr::select(gene, fraction, line, APA_val)


ApaBothFracUTRStand=ApaBothFracUTR_melt %>% full_join(metadata, by=c("line", "fraction")) %>% mutate(StandApa=APA_val/Mapped_noMP)

ApaBothFracUTRStand_geneMean=ApaBothFracUTRStand %>% group_by(fraction, gene) %>% summarise(meanAPA=mean(StandApa, na.rm=T))

ApaBothFracUTRStand_geneMean_spread= spread(ApaBothFracUTRStand_geneMean,fraction,meanAPA ) %>% mutate(APAValLoc=nuclear/total)
ApaBothFracUTRStand_geneMean_spread2= spread(ApaBothFracUTRStand_geneMean,fraction,meanAPA ) %>% mutate(APAValLoc=nuclear/(total+nuclear))

THis is nuclear vs total only looking at teh UTR:

APAUTRandTranscrption= Transcription %>% inner_join(ApaBothFracUTRStand_geneMean_spread, by="gene")
APAUTRandTranscrption$density <- get_density(APAUTRandTranscrption$APAValLoc, APAUTRandTranscrption$Transcription, n = 100)


summary(lm(data=APAUTRandTranscrption, log10(APAValLoc)~log10(Transcription)))

Call:
lm(formula = log10(APAValLoc) ~ log10(Transcription), data = APAUTRandTranscrption)

Residuals:
     Min       1Q   Median       3Q      Max 
-0.69221 -0.18902 -0.00244  0.19660  0.90638 

Coefficients:
                     Estimate Std. Error t value Pr(>|t|)    
(Intercept)          0.155656   0.008892   17.50   <2e-16 ***
log10(Transcription) 0.485080   0.028924   16.77   <2e-16 ***
---
Signif. codes:  0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1

Residual standard error: 0.252 on 7753 degrees of freedom
Multiple R-squared:  0.03501,   Adjusted R-squared:  0.03488 
F-statistic: 281.3 on 1 and 7753 DF,  p-value: < 2.2e-16

ggplot(APAUTRandTranscrption, aes(x=Transcription, y=APAValLoc))+ geom_point(aes(color=density)) + geom_smooth(method = "lm") + labs(x="4su/RNA", y="NuclearUTR/TotalUTR", title="Relationship between APA fraction and transcription") + scale_color_viridis()

Past versions of unnamed-chunk-23-1.png

APAUTRandTranscrption2= Transcription %>% inner_join(ApaBothFracUTRStand_geneMean_spread2, by="gene")
APAUTRandTranscrption2$density <- get_density(APAUTRandTranscrption2$APAValLoc, APAUTRandTranscrption2$Transcription, n = 100)


summary(lm(data=APAUTRandTranscrption2, log10(APAValLoc)~log10(Transcription)))

Call:
lm(formula = log10(APAValLoc) ~ log10(Transcription), data = APAUTRandTranscrption2)

Residuals:
     Min       1Q   Median       3Q      Max 
-0.45055 -0.08567  0.01585  0.10118  0.37283 

Coefficients:
                     Estimate Std. Error t value Pr(>|t|)    
(Intercept)          -0.23370    0.00447  -52.29   <2e-16 ***
log10(Transcription)  0.26974    0.01454   18.55   <2e-16 ***
---
Signif. codes:  0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1

Residual standard error: 0.1267 on 7753 degrees of freedom
Multiple R-squared:  0.04251,   Adjusted R-squared:  0.04239 
F-statistic: 344.2 on 1 and 7753 DF,  p-value: < 2.2e-16

ggplot(APAUTRandTranscrption2, aes(x=Transcription, y=APAValLoc))+ geom_point(aes(color=density)) + geom_smooth(method = "lm") + labs(x="4su/RNA+4su", y="NuclearUTR/TotalUTR+NuclearUTR", title="Relationship between APA fraction and transcription") + scale_color_viridis()

Past versions of unnamed-chunk-24-1.png

Intron Nuclear over all nuclear

Nuclear intron= NucAPAIntron_bygene

all nuclear =NucAPA_bygene

Create this pheno:

ApaNuclear_byloc=NucAPAIntron_bygene %>% inner_join(NucAPA_bygene, by=c("gene", "Individual")) %>% mutate(IntronOverAll=NuclearIntronSum/NuclearSum) %>% mutate(fraction="nuclear",line=paste("NA", substring(Individual, 2), sep="")) %>% dplyr::select(gene, fraction, line, IntronOverAll) %>% group_by(gene) %>% filter(IntronOverAll!=0) %>%  summarise(MeanIntronoverAll=mean(IntronOverAll)) %>% dplyr::rename("GeneName"=gene)

Join with RNA

nuclearandRNA=ApaNuclear_byloc %>% inner_join(RNA_geneMean, by="GeneName")

nuclearandRNA$density <- get_density(nuclearandRNA$MeanIntronoverAll, nuclearandRNA$Mean_RNA, n = 100)

Plot:

ggplot(nuclearandRNA, aes(x=log10(Mean_RNA), y=MeanIntronoverAll))+ geom_point(aes(color=density)) + geom_smooth(method = "lm") + labs(x="log10(RNA)", y="NuclearIntron/NuclearAll", title="Relationship between APA fraction and transcription") + scale_color_viridis()

Past versions of unnamed-chunk-27-1.png

summary(lm(data=nuclearandRNA, MeanIntronoverAll~log10(Mean_RNA)))

Call:
lm(formula = MeanIntronoverAll ~ log10(Mean_RNA), data = nuclearandRNA)

Residuals:
     Min       1Q   Median       3Q      Max 
-0.39640 -0.15292 -0.04646  0.11444  0.84926 

Coefficients:
                 Estimate Std. Error t value Pr(>|t|)    
(Intercept)     -0.408362   0.024437  -16.71   <2e-16 ***
log10(Mean_RNA) -0.159782   0.005852  -27.30   <2e-16 ***
---
Signif. codes:  0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1

Residual standard error: 0.2018 on 5560 degrees of freedom
Multiple R-squared:  0.1182,    Adjusted R-squared:  0.1181 
F-statistic: 745.5 on 1 and 5560 DF,  p-value: < 2.2e-16

ApaNuclear_byloc_rename=ApaNuclear_byloc %>% dplyr::rename("gene"=GeneName)
nuclearandtranscription=ApaNuclear_byloc_rename %>% inner_join(Transcription, by="gene")

nuclearandtranscription$density <- get_density(nuclearandtranscription$MeanIntronoverAll, nuclearandtranscription$Transcription, n = 100)

ggplot(nuclearandtranscription, aes(x=Transcription, y=MeanIntronoverAll))+ geom_point(aes(color=density)) + geom_smooth(method = "lm") + labs(x="4su/4su+RNA", y="NuclearIntron/NuclearAll", title="Relationship between APA fraction and transcription") + scale_color_viridis()

summary(lm(data=nuclearandtranscription, MeanIntronoverAll~Transcription))

Call:
lm(formula = MeanIntronoverAll ~ Transcription, data = nuclearandtranscription)

Residuals:
     Min       1Q   Median       3Q      Max 
-0.29935 -0.17119 -0.05628  0.12082  0.79573 

Coefficients:
              Estimate Std. Error t value Pr(>|t|)    
(Intercept)    0.14461    0.01530   9.452  < 2e-16 ***
Transcription  0.20500    0.02797   7.329 2.66e-13 ***
---
Signif. codes:  0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1

Residual standard error: 0.2138 on 5560 degrees of freedom
Multiple R-squared:  0.009567,  Adjusted R-squared:  0.009389 
F-statistic: 53.71 on 1 and 5560 DF,  p-value: 2.658e-13

Intron Total over all Total

First I need to get the total intronic:

TotAPAIntron=read.table("../data/peakCoverage/APAPeaks.ALLChrom.Filtered.Named.GeneLocAnnoPARSED.Total.Quant.Fixed.fc", stringsAsFactors = F, header = T) %>% dplyr::select(-Chr, -Start, -End, -Strand, -Length) %>% separate(Geneid, into=c("peakid","chrom", "start", "end", "strand", "geneID"),sep=":") %>% semi_join(peaknumlist, by="peakid") %>% separate(geneID, into=c("gene", "loc"), sep="_") %>%filter(loc=="intron") %>%  dplyr::select(-chrom , -start, -end, -strand, -loc)

TotAPAIntronMelt=melt(TotAPAIntron, id.vars =c( "peakid", "gene"), value.name="count", variable.name="Ind") %>% separate(Ind, into=c('Individual', 'fraction') ,sep="_")%>% dplyr::select(peakid, gene, Individual, count)


TotAPAIntron_bygene= TotAPAIntronMelt %>% group_by(gene,Individual) %>% summarise(TotalIntronSum=sum(count))

ApaTotal_byloc=TotAPAIntron_bygene %>% inner_join(TotAPA_bygene, by=c("gene", "Individual")) %>% mutate(IntronOverAll=TotalIntronSum/TotalSum) %>% mutate(fraction="total",line=paste("NA", substring(Individual, 2), sep="")) %>% dplyr::select(gene, fraction, line, IntronOverAll) %>% group_by(gene) %>% filter(IntronOverAll!=0) %>%  summarise(MeanIntronoverAll=mean(IntronOverAll)) %>% dplyr::rename("GeneName"=gene)

Join with RNA

totalandRNA=ApaTotal_byloc %>% inner_join(RNA_geneMean, by="GeneName")

totalandRNA$density <- get_density(totalandRNA$MeanIntronoverAll, totalandRNA$Mean_RNA, n = 100)

Plot:

ggplot(totalandRNA, aes(x=log10(Mean_RNA), y=MeanIntronoverAll))+ geom_point(aes(color=density)) + geom_smooth(method = "lm") + labs(x="log10(RNA)", y="TotalIntron/TotalAll", title="Relationship between APA fraction and transcription") + scale_color_viridis()

Past versions of unnamed-chunk-33-1.png

summary(lm(data=totalandRNA, MeanIntronoverAll~log10(Mean_RNA)))

Call:
lm(formula = MeanIntronoverAll ~ log10(Mean_RNA), data = totalandRNA)

Residuals:
     Min       1Q   Median       3Q      Max 
-0.30265 -0.10190 -0.03972  0.05381  0.92485 

Coefficients:
                 Estimate Std. Error t value Pr(>|t|)    
(Intercept)     -0.445003   0.019435  -22.90   <2e-16 ***
log10(Mean_RNA) -0.145939   0.004654  -31.36   <2e-16 ***
---
Signif. codes:  0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1

Residual standard error: 0.1605 on 5560 degrees of freedom
Multiple R-squared:  0.1503,    Adjusted R-squared:  0.1501 
F-statistic: 983.2 on 1 and 5560 DF,  p-value: < 2.2e-16

ApaTotal_byloc_rename=ApaTotal_byloc %>% dplyr::rename("gene"=GeneName)
totalandtranscription=ApaTotal_byloc_rename %>% inner_join(Transcription, by="gene")

totalandtranscription$density <- get_density(totalandtranscription$MeanIntronoverAll, totalandtranscription$Transcription, n = 100)

ggplot(totalandtranscription, aes(x=Transcription, y=MeanIntronoverAll))+ geom_point(aes(color=density)) + geom_smooth(method = "lm") + labs(x="4su/4su+RNA", y="TotalIntron/TotalAll", title="Relationship between APA fraction and transcription") + scale_color_viridis()

summary(lm(data=totalandtranscription, MeanIntronoverAll~Transcription))

Call:
lm(formula = MeanIntronoverAll ~ Transcription, data = totalandtranscription)

Residuals:
     Min       1Q   Median       3Q      Max 
-0.19578 -0.11811 -0.05860  0.05286  0.87881 

Coefficients:
              Estimate Std. Error t value Pr(>|t|)    
(Intercept)    0.07454    0.01240   6.012 1.95e-09 ***
Transcription  0.16028    0.02267   7.070 1.74e-12 ***
---
Signif. codes:  0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1

Residual standard error: 0.1733 on 5560 degrees of freedom
Multiple R-squared:  0.00891,   Adjusted R-squared:  0.008732 
F-statistic: 49.99 on 1 and 5560 DF,  p-value: 1.739e-12

Put it all together:

(Nuclear intronic/nuclear all)/(total intronic/total all) vs 4su/(4su+RNA)

Nucintron v nuc all:

ApaNuclear_byloc_rename

ApaTotal_byloc_rename

Transcription

fullapa=ApaNuclear_byloc_rename %>% dplyr::rename("NuclearIntronoverall"=MeanIntronoverAll)%>% inner_join(ApaTotal_byloc_rename, by="gene") %>% mutate(fullAPA=NuclearIntronoverall/MeanIntronoverAll) %>% dplyr::select(gene,fullAPA)


#join with transcription 


BothlocPhenoandtranscription=fullapa %>% inner_join(Transcription, by="gene")

BothlocPhenoandtranscription$density <- get_density(BothlocPhenoandtranscription$fullAPA, BothlocPhenoandtranscription$Transcription, n = 100)

ggplot(BothlocPhenoandtranscription, aes(x=Transcription, y=fullAPA))+ geom_point(aes(color=density)) + geom_smooth(method = "lm") + labs(x="4su/4su+RNA", y="(NuclearIntron/Nuclearall)/(TotalIntron/TotalAll)", title="Relationship between APA fraction and transcription") + scale_color_viridis()

summary(lm(data=totalandtranscription, MeanIntronoverAll~Transcription))

Call:
lm(formula = MeanIntronoverAll ~ Transcription, data = totalandtranscription)

Residuals:
     Min       1Q   Median       3Q      Max 
-0.19578 -0.11811 -0.05860  0.05286  0.87881 

Coefficients:
              Estimate Std. Error t value Pr(>|t|)    
(Intercept)    0.07454    0.01240   6.012 1.95e-09 ***
Transcription  0.16028    0.02267   7.070 1.74e-12 ***
---
Signif. codes:  0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1

Residual standard error: 0.1733 on 5560 degrees of freedom
Multiple R-squared:  0.00891,   Adjusted R-squared:  0.008732 
F-statistic: 49.99 on 1 and 5560 DF,  p-value: 1.739e-12

Session information

sessionInfo()

R version 3.5.1 (2018-07-02)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Scientific Linux 7.4 (Nitrogen)

Matrix products: default
BLAS/LAPACK: /software/openblas-0.2.19-el7-x86_64/lib/libopenblas_haswellp-r0.2.19.so

locale:
 [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C              
 [3] LC_TIME=en_US.UTF-8        LC_COLLATE=en_US.UTF-8    
 [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
 [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                 
 [9] LC_ADDRESS=C               LC_TELEPHONE=C            
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
 [1] viridis_0.5.1     viridisLite_0.3.0 forcats_0.3.0    
 [4] stringr_1.3.1     dplyr_0.8.0.1     purrr_0.3.2      
 [7] readr_1.3.1       tidyr_0.8.3       tibble_2.1.1     
[10] ggplot2_3.1.1     tidyverse_1.2.1   workflowr_1.3.0  
[13] reshape2_1.4.3   

loaded via a namespace (and not attached):
 [1] tidyselect_0.2.5 haven_1.1.2      lattice_0.20-38  colorspace_1.3-2
 [5] generics_0.0.2   htmltools_0.3.6  yaml_2.2.0       rlang_0.3.1     
 [9] pillar_1.3.1     glue_1.3.0       withr_2.1.2      modelr_0.1.2    
[13] readxl_1.1.0     plyr_1.8.4       munsell_0.5.0    gtable_0.2.0    
[17] cellranger_1.1.0 rvest_0.3.2      evaluate_0.12    labeling_0.3    
[21] knitr_1.20       broom_0.5.1      Rcpp_1.0.0       scales_1.0.0    
[25] backports_1.1.2  jsonlite_1.6     fs_1.2.6         gridExtra_2.3   
[29] hms_0.4.2        digest_0.6.18    stringi_1.2.4    grid_3.5.1      
[33] rprojroot_1.3-2  cli_1.0.1        tools_3.5.1      magrittr_1.5    
[37] lazyeval_0.2.1   crayon_1.3.4     whisker_0.3-2    pkgconfig_2.0.2 
[41] MASS_7.3-51.1    xml2_1.2.0       lubridate_1.7.4  assertthat_0.2.0
[45] rmarkdown_1.10   httr_1.3.1       rstudioapi_0.10  R6_2.3.0        
[49] nlme_3.1-137     git2r_0.23.0     compiler_3.5.1