Last updated: 2018-12-10

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Briana Mittleman

This is the site I will use for my human 3’ seq project. I will have my workflow and analysis here. The three prime seq protocol I am using is Lexogen, QuantSeq 3’ mRNA-Seq library kit. The first analysis will be on the total and nucleic fractions of the LCL used in the net-seq analysis found at https://brimittleman.github.io/Net-seq/

Initial analysis on RNA seq of nuclear and total fractions:

Explore and filter mis-primming:

Peak calling:

Rerun analysis opp strand:

Evaluate and learn from QTLs:

Analysis for all 39 indivduals:

Molecular QTL analysis and Overlap:

Until now I have been focusing on way to develop the story for the paper. The next steps will be to systematically understand the data in order to filter to high quality data to use in the final analysis.




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