Last updated: 2018-10-08

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Briana Mittleman

This is the site I will use for my human 3’ seq project. I will have my workflow and analysis here. The three prime seq protocol I am using is Lexogen, QuantSeq 3’ mRNA-Seq library kit. The first analysis will be on the total and nucleic fractions of the LCL used in the net-seq analysis found at https://brimittleman.github.io/Net-seq/

Initial analysis on RNA seq of nuclear and total fractions:

Initial analysis on first 32 3’ seq libraries:

Explore and filter mis-primming:

Peak calling:

Peak coverage/Nominal QTLs/Diff iso:

Rerun analysis opp strand:

Evaluate and learn from QTLs:

Analysis for all 39 indivduals:

Molecular QTL analysis and Overlap:




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