Human dermal fibroblast clonality project

Last updated: 2018-08-17

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File	Version	Author	Date	Message
Rmd	6b5f8c7	davismcc	2018-08-17	Updating organisational pages.
Rmd	1cbadbd	davismcc	2018-08-10	Updating analyses.
html	2531565	davismcc	2018-08-08	Tweaking clone prevalences
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html	9856275	davismcc	2018-08-07	Build site.
Rmd	5fc189d	davismcc	2018-08-07	Start workflowr project.

Project overview

This project investigates clonality in human dermal fibroblast cell populations in 32 cell lines from distinct donors, using bulk whole-exome sequencing and single-cell RNA-sequencing data.

Key findings:

A novel approach for integrating DNA-seq and single-cell RNA-seq data to reconstruct clonal substructure and single-cell transcriptomes.
A new computational method, cardelino, to map single-cell RNA-seq profiles to clones.
Evidence for non-neutral evolution of clonal populations in human fibroblasts.
Proliferation and cell cycle pathways are commonly distorted in mutated clonal populations, with implications for cancer and ageing.

For a richer overview, see the About page.

Analyses

Here we present the reproducible the results of our analyses. They were generated by rendering the R Markdown documents into webpages.

The results presented in the paper were produced with these analyses.

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

This reproducible R Markdown analysis was created with workflowr 1.1.1

Human dermal fibroblast clonality project

Davis J. McCarthy

Project overview

Analyses