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These samples (63 in total) have been aligned to the Ensembl 113 release of GRCh38 via subread, and counts have been generated using featureCounts with inbuilt hg38 genome
Data is located under 5FU Project on my computer All analysis saved in RDirectory Recovery_RNAseq
Individual 1 - 84-1 (9 samples)
Individual 2 - 87-1 (9 samples)
Individual 3 - 75-1 (9 samples)
Individual 4 - 75-1 (9 samples)
Individual 5 - 17-3 (9 samples)
Individual 6 - 90-1 (9 samples)
Individual 7 - 90-1 REP (9 samples)
Now that I have imported all of these counts files, I will make a matrix with all of the samples
First I’ll break them up by individual, and then combine them all together
Now that I’ve made individual matrices for each individual - I will combine them together into one large matrix
Version | Author | Date |
---|---|---|
a07d5bc | emmapfort | 2025-03-19 |
Version | Author | Date |
---|---|---|
a07d5bc | emmapfort | 2025-03-19 |
#Now that I've put together my matrix - I will do some QC checks
#now that I have all of my factors and my gene info, I will make some graphs to show the QC data
reads_by_sample <- c(tx_col)
fC_AllCounts %>%
ggplot(., aes (x = Conditions, y = Total_Align, fill = Treatment, group_by = Line))+
geom_col()+
geom_hline(aes(yintercept=20000000))+
scale_fill_manual(values=reads_by_sample)+
ggtitle(expression("Total number of reads by sample"))+
xlab("")+
ylab(expression("RNA-sequencing reads"))+
theme_bw()+
theme(plot.title = element_text(size = rel(2), hjust = 0.5),
axis.title = element_text(size = 15, color = "black"),
axis.ticks = element_line(linewidth = 1.5),
axis.line = element_line(linewidth = 1.5),
axis.text.y = element_text(size =10, color = "black", angle = 0, hjust = 0.8, vjust = 0.5),
axis.text.x = element_text(size =10, color = "black", angle = 90, hjust = 1, vjust = 0.2),
#strip.text.x = element_text(size = 15, color = "black", face = "bold"),
strip.text.y = element_text(color = "white"))
Version | Author | Date |
---|---|---|
a07d5bc | emmapfort | 2025-03-19 |
####Read Counts by Treatment####
fC_AllCounts %>%
ggplot(., aes (x =Treatment, y= Total_Align, fill = Treatment))+
geom_boxplot()+
scale_fill_manual(values=reads_by_sample)+
ggtitle(expression("Total number of reads by treatment"))+
xlab("")+
ylab(expression("RNA-sequencing reads"))+
theme_bw()+
theme(plot.title = element_text(size = rel(2), hjust = 0.5),
axis.title = element_text(size = 15, color = "black"),
axis.ticks = element_line(linewidth = 1.5),
axis.line = element_line(linewidth = 1.5),
axis.text.y = element_text(size =10, color = "black", angle = 0, hjust = 0.8, vjust = 0.5),
axis.text.x = element_text(size =10, color = "black", angle = 90, hjust = 1, vjust = 0.2),
#strip.text.x = element_text(size = 15, color = "black", face = "bold"),
strip.text.y = element_text(color = "white"))
Version | Author | Date |
---|---|---|
a07d5bc | emmapfort | 2025-03-19 |
####Total Reads Per Individual####
fC_AllCounts %>%
ggplot(., aes (x =as.factor(Line), y=Total_Align))+
geom_boxplot(aes(fill=as.factor(Line)))+
scale_fill_brewer(palette = "Dark2", name = "Individual")+
ggtitle(expression("Total number of reads by individual"))+
xlab("")+
ylab(expression("RNA-sequencing reads"))+
theme_bw()+
theme(plot.title = element_text(size = rel(2), hjust = 0.5),
axis.title = element_text(size = 15, color = "black"),
axis.ticks = element_line(linewidth = 1.5),
axis.line = element_line(linewidth = 1.5),
axis.text.y = element_text(size =10, color = "black", angle = 0, hjust = 0.8, vjust = 0.5),
axis.text.x = element_text(size =10, color = "black", angle = 0, hjust = 1),
#strip.text.x = element_text(size = 15, color = "black", face = "bold"),
strip.text.y = element_text(color = "white"))
Version | Author | Date |
---|---|---|
a07d5bc | emmapfort | 2025-03-19 |
####Total Mapped Reads Per Drug####
reads_by_sample <- c(tx_col)
fC_AllCounts %>%
ggplot(., aes (x = Conditions, y = Assigned_Align, fill = Treatment, group_by = Line))+
geom_col()+
geom_hline(aes(yintercept=20000000))+
scale_fill_manual(values=reads_by_sample)+
ggtitle(expression("Total number of mapped reads by sample"))+
xlab("")+
ylab(expression("RNA-sequencing reads"))+
theme_bw()+
theme(plot.title = element_text(size = rel(2), hjust = 0.5),
axis.title = element_text(size = 15, color = "black"),
axis.ticks = element_line(linewidth = 1.5),
axis.line = element_line(linewidth = 1.5),
axis.text.y = element_text(size =10, color = "black", angle = 0, hjust = 0.8, vjust = 0.5),
axis.text.x = element_text(size =10, color = "black", angle = 90, hjust = 1, vjust = 0.2),
#strip.text.x = element_text(size = 15, color = "black", face = "bold"),
strip.text.y = element_text(color = "white"))
Version | Author | Date |
---|---|---|
a07d5bc | emmapfort | 2025-03-19 |
####Read Counts by Treatment####
fC_AllCounts %>%
ggplot(., aes (x =Treatment, y= Assigned_Align, fill = Treatment))+
geom_boxplot()+
scale_fill_manual(values=reads_by_sample)+
ggtitle(expression("Total number of mapped reads by treatment"))+
xlab("")+
ylab(expression("RNA-sequencing reads"))+
theme_bw()+
theme(plot.title = element_text(size = rel(2), hjust = 0.5),
axis.title = element_text(size = 15, color = "black"),
axis.ticks = element_line(linewidth = 1.5),
axis.line = element_line(linewidth = 1.5),
axis.text.y = element_text(size =10, color = "black", angle = 0, hjust = 0.8, vjust = 0.5),
axis.text.x = element_text(size =10, color = "black", angle = 90, hjust = 1, vjust = 0.2),
#strip.text.x = element_text(size = 15, color = "black", face = "bold"),
strip.text.y = element_text(color = "white"))
Version | Author | Date |
---|---|---|
a07d5bc | emmapfort | 2025-03-19 |
####Total Reads Per Individual####
fC_AllCounts %>%
ggplot(., aes (x =as.factor(Line), y=Assigned_Align))+
geom_boxplot(aes(fill=as.factor(Line)))+
scale_fill_brewer(palette = "Dark2", name = "Individual")+
ggtitle(expression("Total number of mapped reads by individual"))+
xlab("")+
ylab(expression("RNA-sequencing reads"))+
theme_bw()+
theme(plot.title = element_text(size = rel(2), hjust = 0.5),
axis.title = element_text(size = 15, color = "black"),
axis.ticks = element_line(linewidth = 1.5),
axis.line = element_line(linewidth = 1.5),
axis.text.y = element_text(size =10, color = "black", angle = 0, hjust = 0.8, vjust = 0.5),
axis.text.x = element_text(size =10, color = "black", angle = 0, hjust = 1),
#strip.text.x = element_text(size = 15, color = "black", face = "bold"),
strip.text.y = element_text(color = "white"))
Version | Author | Date |
---|---|---|
a07d5bc | emmapfort | 2025-03-19 |
Now that I’ve made the final full matrix of all samples - 1. convert to log2cpm using cpm 2. filter for lowly expressed reads (rowMeans > 0)
fC_Matrix_Full
DOX_24_Ind1 FLUO_24_Ind1 DMSO_24_Ind1 DOX_24rec_Ind1 FLUO_24rec_Ind1
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57540 151 6 8 55 6
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10459 445 316 262 438 263
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57085 747 491 350 659 311
284498 3 0 1 6 1
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1185 1073 1619 1430 1816 1304
100379251 33 43 39 67 46
4878 435616 251903 193879 132175 185340
4879 90330 87916 64788 39173 59690
26829 20 1 8 15 2
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55187 896 1674 1577 1681 1249
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148581 13 15 12 11 10
57685 1630 3142 2566 1924 2188
100616338 0 0 0 0 0
55225 1164 1504 1440 529 1128
3716 3255 2133 1945 2731 1594
101927084 0 0 0 1 0
105378775 10 0 0 12 1
100500854 0 0 0 3 1
406893 0 0 0 0 2
205 1208 493 455 369 427
9829 1005 3136 2823 1406 2185
54741 4436 3667 3242 2688 2757
3953 318 277 205 298 237
5142 28 392 414 400 275
101927139 0 0 0 0 0
84251 34 39 41 102 36
100422871 0 0 1 0 0
200132 2 1 3 2 0
10022 19 0 1 6 0
79819 215 75 57 85 57
57708 894 823 838 1405 661
23169 1029 554 428 573 454
400757 1 0 0 0 0
149233 0 0 0 4 0
3595 30 5 2 32 4
26135 3707 6076 5367 6003 4279
1647 3953 3347 2349 7503 2651
55970 1954 3672 3134 3024 2922
100289178 51 68 75 52 53
9077 119 188 160 285 120
79971 12107 16304 13598 10621 12646
100302279 0 0 0 0 0
6121 5 3 2 0 0
55635 445 509 787 123 50
101927220 1 1 1 1 3
57554 14 94 66 73 128
5301 0 1 0 1 2
55631 434 682 701 740 515
9295 3228 5415 4834 5810 4308
81573 778 1146 937 1099 931
11147 169 123 109 152 108
1491 113 152 136 150 103
101927244 4 0 0 0 0
5733 106 60 52 76 37
100132618 42 112 72 64 60
9406 2521 3109 2934 4101 2559
406962 13 45 25 37 24
100852410 3 7 7 11 4
257194 242 453 325 309 356
100852409 0 0 1 0 0
101927295 1 0 0 0 0
127255 1 6 2 12 2
100526835 7 21 23 8 15
8790 68 335 336 244 255
51086 25 64 52 8 57
105378804 16 3 7 2 6
105378803 0 0 5 0 3
127254 14 1 2 7 0
101927320 0 0 0 0 0
1429 1084 869 769 1191 657
127253 473 464 446 534 379
431707 8 4 4 5 5
204962 439 439 391 231 320
34 2281 1975 1890 1854 1623
1744 131 182 151 159 134
5876 578 1585 1427 2113 1378
692085 10 16 24 30 12
26805 11 18 11 32 9
26804 13 12 16 15 8
4438 22 5 0 27 4
127247 0 0 0 0 0
101927342 0 0 0 4 0
256435 859 1148 924 329 839
81849 38 124 76 49 165
102466995 0 0 0 0 0
10026 1950 1572 1386 1049 1180
26289 11 9 3 27 7
26009 398 1080 1027 1307 777
23032 1494 1884 1702 2347 1397
374986 692 884 813 852 649
374987 110 33 15 100 16
91624 5774 17792 15563 11259 12245
8880 987 2527 2586 3496 2196
11080 2027 1932 1824 2607 1661
54810 59 30 30 46 22
149047 2 0 1 0 0
5737 10 0 2 9 4
10964 1 0 0 4 0
10561 3 3 0 6 3
64123 169 432 323 287 282
101927412 2 0 0 0 0
23266 1941 4820 4290 3843 3457
101927434 2 0 0 0 0
101927498 6 2 3 7 3
101927587 1 0 1 2 0
101927560 0 0 2 0 0
79739 556 640 492 371 551
5567 1551 1876 1728 1628 1372
148418 4 4 20 12 9
391051 9 0 2 14 2
58511 0 0 0 5 0
80135 800 811 687 1310 648
2787 1406 2511 2388 2948 2462
100505741 39 25 15 21 17
1486 926 931 764 1087 601
103695366 1 1 0 0 2
439927 21 1 1 0 0
117178 1119 794 724 932 558
23566 214 100 127 93 79
255231 60 7 7 9 4
55283 594 40 52 198 24
126820 561 260 177 527 193
100616481 6 0 1 0 0
84144 454 482 375 467 321
148423 1016 724 578 1153 602
8915 492 304 300 599 300
646626 12 4 1 9 3
23576 3241 4668 3964 3224 4279
3491 3699 20402 15179 18020 15847
54680 290 578 534 811 509
255631 25 8 7 49 16
57489 608 975 880 973 783
102465842 0 1 0 3 1
9635 55 7 7 217 22
1179 1 0 1 6 0
22802 7 2 0 7 0
9629 1 3 0 0 1
105378828 97 30 35 81 37
51100 2038 6601 5963 7068 5444
9403 3029 2527 2149 2904 2026
9653 3166 5848 4950 4811 3919
339524 54 15 15 40 7
101927844 1 0 0 0 0
105378832 3 0 0 0 0
8543 1482 3271 2847 3483 2783
100505768 0 2 2 3 0
101927891 6 6 2 7 1
5586 879 3019 2922 2516 2205
2959 1791 510 474 826 439
56267 156 267 211 322 218
494115 205 709 685 740 504
2635 149 560 344 433 418
2633 6 19 11 9 17
2634 51 166 106 87 108
388646 2 1 3 1 0
115361 6 60 32 10 37
115362 1 0 1 0 2
729930 1 0 0 7 1
163351 2 3 5 8 1
400759 0 0 0 0 0
23507 459 1401 1258 989 928
84230 927 984 946 669 688
400761 28 26 16 15 22
55144 802 2083 1854 1571 1512
105378846 0 0 0 0 0
492303 37 6 19 7 7
284695 523 1116 983 1181 831
343472 17 0 0 1 0
84146 1178 1678 1454 2064 1375
164045 9 43 16 27 29
8317 226 111 181 159 86
7049 377 145 112 67 78
676 4 0 0 1 1
253152 31 84 79 66 73
646817 48 63 39 95 60
284697 4 18 13 23 21
23285 36 118 118 122 125
388649 2 3 2 4 3
11146 183 416 366 366 323
79871 203 356 330 459 296
2672 282 10 4 20 8
7813 827 1454 1213 1271 1035
6125 8243 8480 7375 9864 7564
388650 504 810 720 484 647
6083 5 3 5 4 2
26782 7 6 3 7 8
22823 546 598 655 1012 448
50999 5094 5427 4717 3404 3390
343099 228 134 164 230 79
100131564 566 461 448 992 480
1810 2359 1439 1234 2011 963
54874 1141 1441 1183 1785 1289
100126520 0 1 0 0 0
8412 296 274 190 492 278
100129046 22 0 1 7 0
100126348 2 0 0 0 0
100189133 14 0 4 1 2
30836 1765 1659 1509 2349 1338
2730 685 541 535 620 365
24 19 2 0 22 0
9411 1346 1132 943 756 813
5825 1244 2317 2142 1903 1834
2152 1216 710 489 676 558
101928079 5 15 2 5 4
100616321 0 0 0 0 0
126969 67 89 52 79 54
1266 5554 4917 4021 5730 3909
105378862 73 25 13 21 17
729970 221 80 96 81 73
199857 112 196 165 191 167
101928098 4 0 1 0 0
148534 2441 2864 2557 1153 1504
100527978 21 40 37 40 26
101928118 4 8 3 12 3
25950 229 398 351 401 320
440594 0 0 0 0 0
100996635 0 0 1 1 0
102723661 0 0 0 0 0
101928241 0 1 0 5 0
58155 526 709 717 910 525
1806 29 70 43 55 58
100873932 0 0 1 0 0
100873933 0 0 0 0 0
400765 6 5 7 7 2
100616452 3 1 0 2 1
406928 0 0 0 0 0
729987 7 1 5 9 2
51375 758 582 514 455 456
163404 372 0 0 3 0
100129620 1 0 0 4 0
9890 37 12 8 23 10
101928270 0 0 0 0 0
54873 11 12 4 25 15
391059 178 444 364 297 309
178 2283 4822 4604 2670 3185
23443 935 1226 1090 1172 840
64645 1580 2010 1687 1849 1513
163786 111 139 172 190 70
54482 176 406 412 617 347
127495 151 935 982 635 771
1629 995 1281 1075 1107 918
100506007 99 50 43 100 23
8634 1395 1772 1502 1848 1448
693138 0 2 0 4 1
8556 593 203 172 249 137
54112 59 3 3 7 2
100128787 0 0 0 0 0
7412 200 1911 1583 290 1231
2135 1326 1314 1173 666 978
148867 1421 1247 917 1344 967
51611 141 583 538 688 440
102606465 51 189 200 248 167
101928334 0 0 0 0 0
101928370 107 198 170 148 146
1901 797 7408 6446 3515 5628
104355286 0 0 0 0 0
118427 4 2 1 3 2
94236 1 0 0 1 1
1301 509 349 238 344 341
101928436 3 0 0 0 2
55599 511 730 661 780 617
280 789 528 453 611 445
648740 22 11 24 30 14
279 43 15 15 21 8
276 34 15 15 17 9
277 3 0 1 1 3
278 6 11 8 8 4
100129138 5 1 1 4 1
101928476 0 0 0 1 0
55170 582 718 727 839 573
22854 28 25 22 25 43
10451 58 13 14 8 6
102465839 0 0 0 0 0
100873946 0 0 0 0 0
29957 1439 1798 1577 1904 1442
148545 3 0 0 2 0
653149 0 0 0 0 0
284611 551 288 246 271 223
113802 102 39 50 120 35
55119 1560 1876 1716 2494 1471
163479 58 8 1 19 3
6814 1287 1408 1218 1874 1144
254268 5 0 0 5 0
642864 4 0 0 2 0
29899 32 45 50 41 18
23155 1649 2203 1826 1437 1604
22911 1404 1081 949 1041 873
6884 808 565 508 836 437
56900 920 1164 1212 1396 1050
677766 15 0 5 6 5
127003 23 0 2 10 0
57535 376 25 15 200 27
6301 2716 3311 2778 5069 2933
1952 2825 1550 1517 1319 1276
84722 105 105 102 72 53
343263 126 142 130 133 129
6272 20519 25394 20125 13337 19948
5686 1898 1652 1423 2459 1348
284612 876 661 615 583 457
127002 273 205 170 323 181
284613 243 398 356 621 335
57463 384 892 817 582 698
83873 39 15 22 18 18
2773 721 1384 1123 1901 1064
406974 3 4 5 0 0
2780 23 46 38 36 27
271 1302 1419 1199 1553 1233
2948 798 782 659 800 680
2946 562 454 380 307 404
2944 21 18 19 25 18
2949 7 7 12 11 10
2947 1635 775 623 781 688
79574 0 1 0 0 0
1435 2812 740 448 1242 470
10768 2913 2174 1953 2437 1695
85369 1258 1741 1519 1906 1435
257 13 22 18 33 19
104355139 5 0 0 0 1
164153 1 0 2 0 0
388662 98 203 149 222 160
100873974 50 1 10 34 2
3749 1059 948 821 588 773
440600 10 4 5 8 4
64783 769 381 317 511 243
9122 49 49 25 45 26
10542 826 1075 938 1555 1020
101410535 16 7 6 17 3
84432 2 7 1 4 5
643160 5 2 3 7 0
440602 7 2 0 7 1
3744 0 0 0 1 0
3737 214 14 16 20 15
3738 137 0 0 7 1
963 3 1 1 12 1
55791 770 568 504 679 473
128338 455 896 844 848 676
10390 792 893 701 897 659
79961 25 2 6 24 2
1117 10 0 0 3 0
149620 4 0 0 0 0
27159 2 0 0 8 0
128344 180 173 211 225 150
441897 2 0 0 4 0
5016 269 38 21 154 25
79084 2023 1777 1486 2395 1402
515 4805 4953 4366 5166 4048
128346 157 21 30 60 19
57413 10 9 5 7 4
140 1 1 0 1 0
100129269 95 54 37 33 53
5906 1427 2119 1707 2170 1816
55924 2141 2649 2367 1721 2238
100506343 112 68 61 48 49
101928718 80 51 28 13 26
11218 510 930 932 849 810
3752 71 91 60 44 57
100874295 0 0 0 0 0
100873995 0 0 0 0 0
643355 59 5 12 21 8
55917 858 1105 926 1454 1042
100422976 0 2 1 0 0
7482 72 41 22 57 24
54879 425 568 539 744 402
829 2674 3667 3301 5761 3261
4343 1632 1643 1395 1777 1448
389 5972 5750 4556 6992 5680
333926 602 102 107 200 68
284467 82 2 1 5 2
100996702 13 13 4 6 9
6566 10717 15096 13501 9020 11176
246182 12 21 6 7 4
100506392 15 34 29 46 24
100996251 13 10 8 22 12
9860 236 610 574 680 457
643441 0 0 0 0 0
260425 451 444 399 286 351
10745 510 1534 1237 1429 997
54665 496 402 381 446 320
100287722 32 19 10 31 9
26191 34 6 3 6 4
440603 16 8 6 17 7
10717 350 580 407 572 407
64858 497 114 106 129 87
101928846 41 5 1 12 7
204851 2583 1591 1471 2081 1155
56944 600 475 374 487 339
148281 22 0 0 2 1
51592 918 1619 1467 2032 1186
10286 572 803 749 1463 705
163259 150 111 134 172 102
270 12 0 0 10 0
4893 1201 1971 1856 2858 1552
7812 15474 14760 13001 16958 11825
80143 1284 1525 1335 1818 1192
6847 0 0 0 2 0
7252 3 4 0 0 0
10100 847 1062 975 635 652
4803 196 137 89 243 132
81839 895 1013 793 591 653
845 42041 32877 27861 13442 25878
4808 257 3 3 112 3
101928995 3 1 1 8 2
101928977 0 0 5 4 0
55356 368 888 777 709 771
126868 23 54 40 27 46
476 33928 44665 40324 31956 33935
84852 24 36 38 62 37
101929023 5 1 0 2 1
965 355 639 568 333 389
100616384 0 0 2 0 0
3321 6552 7013 6437 2349 5038
100302117 3 0 0 0 0
388667 0 0 0 0 0
914 0 0 0 0 0
5738 14024 14994 13508 5618 11814
9398 47 32 30 26 29
101929099 5 0 3 3 1
8458 457 432 411 368 307
100126331 0 0 0 0 0
80263 364 161 199 252 153
79679 14 0 0 4 0
104355292 0 0 0 0 0
10905 1653 3621 3167 1925 2551
100996263 7 0 0 0 0
54855 516 59 52 187 46
54834 599 427 345 521 326
10885 1429 1137 961 1140 855
200162 14 1 0 8 2
6913 7 0 1 2 0
10352 248 270 276 262 197
104472716 2 0 0 0 0
101929147 81 90 86 111 94
51179 2 0 0 2 0
100874270 0 0 0 0 0
3284 1 17 17 0 12
3283 7 1 0 0 0
128102 18 26 17 24 6
644242 54 54 56 72 57
90874 792 1153 1093 1025 1020
26227 1283 1066 944 505 887
3158 0 0 0 2 0
83998 1 0 0 1 0
343505 4 0 0 6 0
11085 1 0 0 1 0
4853 8069 10418 8713 7626 7948
9554 1380 1819 1750 2161 1750
105378954 63 21 17 24 10
728841 709 740 684 909 657
767846 45 47 21 29 24
100996724 1039 2601 2372 1537 2152
101929796 194 187 154 178 147
101060684 1336 1071 971 1078 857
100189005 3 0 0 5 0
101954277 2 0 0 1 0
653505 39 15 14 15 13
728855 164 92 65 106 94
101954264 3 3 1 2 0
100189257 0 0 0 0 2
105369199 2 1 0 2 3
100996721 9 7 17 7 4
100126488 0 0 0 0 0
101929798 0 1 0 0 0
2210 15 3 4 7 2
100996318 7 1 3 2 0
337875 3 1 0 0 0
653820 319 92 82 80 44
653464 700 1146 1028 1144 897
100873165 5 10 9 17 8
647121 22 18 21 18 25
101929815 1 2 0 0 2
100189261 0 0 0 0 0
101954269 0 0 0 0 0
26863 0 1 0 0 0
791227 0 0 0 0 0
105369140 3 3 2 3 1
388692 11 6 4 4 2
100189142 0 0 0 0 0
100189326 0 0 0 0 0
199882 5 0 0 0 0
100189356 0 0 0 0 0
100132417 11 5 2 3 0
100996713 7 2 2 0 1
554282 250 50 68 56 35
100996712 444 608 551 632 522
101060524 2 0 0 0 0
730262 4 8 1 4 9
101954267 1 0 0 1 0
100189323 0 0 0 0 0
284565 718 785 673 803 685
100189127 2 0 0 2 0
728945 2 0 1 3 5
105371215 3 0 0 0 3
1818 3 0 0 0 0
645166 4 17 5 29 12
647135 641 957 798 961 723
728833 284 56 95 53 38
101929805 1 0 0 0 0
102724235 18 11 15 3 8
103091866 83 41 20 42 43
645142 1 2 1 0 0
101954266 2 2 1 8 2
7214 1 1 0 2 0
100288142 332 302 264 370 236
100126510 0 0 0 0 0
101060254 18 41 56 16 37
101929780 197 146 141 215 111
653519 384 465 461 363 334
5174 44 20 14 29 17
11126 22 45 45 50 28
27246 514 1502 1355 1597 1187
10623 597 696 563 892 572
200035 204 90 101 206 117
10401 873 1011 812 1016 904
102466191 0 0 0 0 0
148741 11 2 2 6 2
8515 331 396 326 121 291
8799 237 577 516 576 465
114814 12 25 26 18 27
9939 1583 1596 1368 2829 1289
128077 381 892 823 1065 732
84265 827 684 527 883 568
284615 132 394 337 346 316
10628 2034 4627 4069 4214 4355
148738 13 65 51 26 47
100189384 0 0 0 0 0
100189064 0 0 0 0 0
100188991 0 0 0 0 0
100189198 0 0 0 0 0
101954276 2 1 0 0 0
101928979 10 18 28 44 17
100132406 349 274 256 267 225
388677 229 260 281 352 226
170688 469 1438 1333 1231 1115
105371242 1 0 0 0 0
102724558 0 1 0 3 0
100288805 7 5 8 28 5
105373468 12 17 22 8 20
149013 1033 810 629 916 671
728989 20 59 34 27 32
100189334 0 0 0 0 0
101447996 0 0 0 0 0
644861 1 0 1 2 1
5565 3478 2084 1937 3043 1572
171423 14640 14165 12329 9112 10725
2330 107 132 103 137 101
9557 447 637 594 408 514
100289211 6 37 32 11 26
607 712 794 777 896 710
51205 255 455 412 503 404
2702 127 2002 2129 166 1864
2703 3 0 0 5 1
51463 447 572 550 440 412
100034743 40 17 30 46 11
100189140 2 0 0 0 0
26864 1 0 1 3 0
100189193 1 0 0 0 0
200030 401 319 285 383 276
101927468 0 0 0 0 1
100189056 0 0 1 0 0
100189307 0 0 0 0 0
100189174 0 0 0 0 0
100188998 0 0 2 0 0
100847044 0 0 0 1 0
101954273 0 0 0 1 0
102466225 0 1 0 0 0
26860 0 0 0 0 0
100132057 10 35 30 53 41
101954272 0 1 0 1 1
388685 197 72 72 79 50
644591 8 6 6 0 2
25832 953 842 699 878 551
100996763 399 381 335 397 235
440672 565 1855 1669 1583 1378
9659 3569 8862 8360 4665 6819
400818 465 461 376 522 368
653513 395 910 896 493 823
100996761 19 29 30 23 16
101060226 755 629 575 621 521
653598 41 18 24 34 37
57234 172 72 79 116 68
644634 15 14 12 12 13
100189228 0 0 0 0 0
102723955 5 1 2 0 5
100189059 1 0 0 0 0
440689 133 26 34 65 42
2209 54 7 19 27 16
101927767 24 0 8 9 3
653604 10 1 2 0 2
8370 109 36 47 74 59
105371405 351 88 86 178 97
126961 56 8 1 24 9
8337 451 108 67 443 91
337873 71 14 20 36 10
723790 1381 217 185 1594 247
333932 31 3 4 7 5
554313 52 13 20 18 15
8349 4306 2491 2263 4079 2093
8338 73 1 5 28 6
DMSO_24rec_Ind1 DOX_144rec_Ind1 FLUO_144rec_Ind1 DMSO_144rec_Ind1
100287102 0 0 0 0
653635 198 166 197 251
102466751 0 5 3 13
100302278 2 0 0 0
645520 0 0 0 1
79501 0 0 0 0
729737 177 200 200 251
102725121 0 0 0 0
102723897 201 229 180 257
102465909 2 0 1 0
729759 0 0 0 0
100132287 134 145 146 200
105378947 4 8 1 2
101928626 1 0 0 0
102465432 2456 2647 2606 3190
81399 0 0 0 0
100133331 112 125 135 168
100288069 20 4 16 11
400728 1 0 4 7
79854 31 32 29 49
643837 1289 1169 1365 2134
284593 0 0 0 2
100130417 22 30 23 31
148398 13 30 28 32
26155 1603 1359 1371 1760
339451 445 412 367 576
84069 16 17 14 8
84808 8 11 3 8
57801 1029 641 716 1043
9636 300 223 196 263
375790 23008 14589 16887 23764
105369174 8 7 10 2
105378948 18 2 16 8
401934 1 0 0 2
54991 248 231 278 316
254099 0 0 0 0
406984 0 0 0 0
406983 0 0 0 0
554210 0 0 0 0
254173 0 0 0 0
8784 1 2 0 1
7293 0 0 2 4
51150 6872 6385 6318 8960
126792 1258 1153 1147 1738
388581 19 23 21 41
118424 953 856 815 1192
6339 187 186 217 261
116983 2029 1812 2029 2694
102465434 1 0 0 0
126789 204 184 200 269
54973 1209 1095 1044 1533
102465435 0 1 0 1
80772 532 393 473 724
83756 28 21 27 42
1855 1163 992 1041 1524
102466740 0 0 0 0
54587 640 568 552 763
54998 1083 1002 877 1432
81669 4970 4182 4005 5649
148413 307 259 277 374
55052 971 890 736 1186
441869 1 0 0 1
643965 1 0 0 1
102724312 4 1 5 4
64856 119 74 63 81
219293 20 16 12 19
83858 677 600 654 959
55210 748 596 613 924
339453 44 46 44 66
29101 1629 1746 1366 2060
643988 357 357 303 466
142678 1869 1725 1810 2393
8510 524 473 445 772
984 887 795 638 1077
728661 2338 2226 2200 3198
8511 964 1077 941 1461
728642 785 742 689 1013
9906 1012 975 932 1405
65220 1080 903 1065 1377
2782 7034 5516 5854 9253
105378949 9 16 14 15
163688 6 17 11 11
339456 6 4 1 6
85452 1 1 5 3
2563 0 0 1 0
5590 641 475 499 745
100506504 50 44 38 61
199990 692 624 593 850
105378592 10 9 6 24
6497 1554 1065 1352 2132
105378594 0 0 2 6
79906 105 109 84 128
100129534 21 23 23 26
11079 2675 2546 2514 3451
5192 264 282 243 318
9651 15 15 28 46
55229 822 706 823 1080
388585 0 2 0 2
115110 0 0 0 0
8764 0 0 0 0
100996583 2 2 0 2
127281 358 297 268 438
79258 3 0 3 0
100287898 29 28 26 49
140625 0 0 0 0
440556 76 40 76 92
63976 209 135 216 314
100422968 0 0 0 0
27237 150 98 124 135
1953 2924 2083 2373 3626
693135 0 0 0 1
127262 1395 1202 1133 1886
49856 252 229 217 320
7161 6 8 8 16
57212 777 777 801 1121
148870 24 23 22 44
388588 43 55 40 66
57470 1537 1263 1262 1915
9731 674 579 642 908
1677 80 82 60 122
339448 281 261 263 408
100133612 14 22 13 20
728716 0 0 0 0
284661 1 0 0 0
55966 0 1 0 2
100616489 0 0 0 0
100616421 0 0 0 1
261734 205 127 180 203
8514 52 61 53 65
26038 20 39 52 72
6146 2431 2360 1906 3166
102724450 15 15 21 34
388591 5389 4824 5771 7650
23463 2623 2688 2659 4087
148645 2 0 4 3
390992 0 0 0 0
387509 307 283 297 415
11332 916 580 691 1036
54626 2 14 12 9
83715 11 4 15 4
100422975 0 0 0 0
8718 577 450 542 618
57449 2530 2029 2231 2864
79707 607 579 495 754
80835 1 7 0 1
3104 356 327 311 422
9903 4268 3094 3525 5336
148479 588 512 553 848
90326 187 210 197 255
55735 1036 871 976 1398
100505887 0 0 0 0
23261 582 627 468 852
9341 1824 1768 1277 2300
8863 139 191 177 293
10911 7 0 1 2
3604 84 23 19 33
11315 2819 2638 2138 3694
54206 1769 1060 1218 1671
102724539 4 2 0 2
50651 46 75 70 99
473 3092 2825 3159 4368
102724552 3 0 1 4
2023 12230 9928 9856 13368
102465436 1 0 0 0
100505975 4 14 3 13
765 0 1 0 0
155184 0 0 0 0
6518 7 9 4 6
80045 58 52 67 95
407040 4 2 3 1
9563 1705 1656 1859 2696
80176 200 182 210 325
100506022 10 20 13 16
84275 148 130 136 206
199953 649 536 623 852
5293 257 209 227 307
644997 0 0 0 0
101929074 10 10 8 29
22883 7802 5532 6168 8714
56998 534 542 461 741
84328 434 329 294 498
64802 502 486 503 799
100847055 0 0 0 2
105376719 1 1 1 0
116362 5 5 4 9
10277 1863 1696 1687 2419
23095 3868 3079 3608 5536
100422970 1 0 0 0
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431707 2 5 1 3
204962 388 439 358 660
34 1656 1512 1531 2388
1744 134 134 140 205
5876 1383 1282 1183 1813
692085 13 4 9 6
26805 22 23 9 18
26804 12 13 8 23
4438 4 3 2 4
127247 0 0 0 0
101927342 1 0 0 0
256435 758 551 597 895
81849 105 65 112 179
102466995 0 2 0 0
10026 1178 1085 1073 1677
26289 1 10 8 7
26009 844 678 700 1074
23032 1435 1219 1248 1910
374986 630 528 586 858
374987 25 19 14 30
91624 13388 13793 12355 18788
8880 2210 2026 1933 2971
11080 1596 1438 1391 2310
54810 26 13 14 36
149047 0 0 0 0
5737 1 0 2 1
10964 0 2 0 0
10561 6 1 1 3
64123 204 163 176 239
101927412 0 2 0 0
23266 3579 2981 3082 4697
101927434 0 0 0 0
101927498 1 4 0 4
101927587 0 0 0 0
101927560 0 4 0 0
79739 531 287 347 522
5567 1396 1293 1203 1801
148418 11 6 4 7
391051 2 0 0 1
58511 1 0 0 2
80135 694 725 610 898
2787 2612 2384 1899 3374
100505741 14 3 18 24
1486 630 591 490 820
103695366 0 0 0 1
439927 1 0 0 0
117178 596 463 464 733
23566 125 117 116 203
255231 5 2 5 13
55283 28 74 33 36
126820 147 164 115 171
100616481 0 1 0 1
84144 353 306 298 497
148423 594 488 499 679
8915 246 208 203 332
646626 2 2 3 6
23576 3470 2268 2565 4005
3491 13636 9152 9895 15142
54680 491 414 347 592
255631 6 37 8 14
57489 767 613 618 959
102465842 0 0 0 0
9635 5 12 9 14
1179 0 4 0 0
22802 1 0 0 0
9629 1 1 0 3
105378828 38 27 17 41
51100 4885 3982 4092 6313
9403 1929 1905 1716 2481
9653 3911 2634 3064 4364
339524 17 7 5 11
101927844 0 0 0 0
105378832 0 0 0 0
8543 2787 2706 2295 3623
100505768 0 2 1 0
101927891 0 1 6 6
5586 2468 1879 1956 3090
2959 425 409 361 574
56267 191 204 205 285
494115 475 484 449 743
2635 233 191 180 236
2633 12 1 12 6
2634 116 72 45 89
388646 0 0 1 1
115361 25 12 19 20
115362 3 1 0 1
729930 2 5 0 2
163351 1 1 2 3
400759 0 0 0 0
23507 883 476 538 921
84230 740 484 592 880
400761 29 31 22 30
55144 1599 1529 1298 2023
105378846 0 0 1 1
492303 16 13 15 16
284695 774 744 711 1078
343472 0 0 0 0
84146 1302 1031 1203 1650
164045 19 28 31 42
8317 152 57 142 203
7049 121 69 93 170
676 1 1 0 0
253152 54 45 45 75
646817 56 30 28 51
284697 21 16 13 18
23285 117 85 102 126
388649 2 1 1 2
11146 363 295 257 451
79871 303 271 247 393
2672 7 6 10 15
7813 1094 901 1089 1542
6125 7697 6951 5702 9706
388650 574 547 546 894
6083 4 3 3 4
26782 11 0 5 3
22823 490 424 439 713
50999 3409 2822 3183 4892
343099 147 77 127 155
100131564 496 417 435 465
1810 1121 837 830 1389
54874 1097 910 1172 1682
100126520 0 0 0 0
8412 192 210 226 341
100129046 1 4 0 1
100126348 0 1 0 2
100189133 1 1 2 3
30836 1229 1158 1089 1535
2730 396 325 300 491
24 0 5 1 2
9411 730 461 551 927
5825 2020 1681 1782 2727
2152 505 312 475 648
101928079 13 2 4 4
100616321 0 0 0 0
126969 46 30 29 51
1266 3555 3349 2959 5061
105378862 23 9 16 22
729970 74 95 55 118
199857 178 160 140 197
101928098 0 2 0 0
148534 1825 1685 1887 3145
100527978 37 31 25 55
101928118 1 5 5 4
25950 357 328 255 429
440594 0 0 0 0
100996635 0 0 0 2
102723661 0 0 0 0
101928241 0 0 0 0
58155 520 618 533 785
1806 41 33 45 70
100873932 0 0 0 0
100873933 0 0 0 0
400765 9 4 8 14
100616452 0 0 0 1
406928 0 0 0 0
729987 1 5 2 11
51375 424 323 309 553
163404 0 6 0 1
100129620 0 1 0 0
9890 5 8 6 10
101928270 0 0 0 0
54873 9 14 38 44
391059 216 175 169 280
178 3926 3175 3807 5816
23443 752 685 705 1057
64645 1476 1385 1232 1919
163786 149 80 116 214
54482 387 360 312 446
127495 854 1109 906 1523
1629 887 819 857 1359
100506007 40 29 30 42
8634 1407 1263 1059 1808
693138 3 0 0 0
8556 143 107 82 186
54112 1 8 2 0
100128787 0 0 0 0
7412 1572 941 1333 1778
2135 1127 841 949 1368
148867 792 590 721 989
51611 537 413 413 631
102606465 160 155 139 244
101928334 2 0 0 1
101928370 159 108 151 216
1901 5365 4548 5617 8498
104355286 0 0 0 0
118427 1 5 0 0
94236 0 2 0 0
1301 348 284 406 482
101928436 0 1 1 0
55599 651 708 681 891
280 529 589 486 707
648740 17 19 19 19
279 2 12 15 17
276 13 6 12 20
277 2 4 1 3
278 11 4 3 14
100129138 3 0 2 3
101928476 0 0 0 0
55170 662 496 466 770
22854 36 10 17 52
10451 10 15 13 5
102465839 0 0 0 0
100873946 0 0 0 0
29957 1246 944 1058 1631
148545 0 0 0 0
653149 0 0 0 0
284611 216 158 185 314
113802 41 30 22 39
55119 1506 1372 1298 1836
163479 2 0 1 3
6814 1148 975 813 1307
254268 0 0 0 0
642864 0 0 0 1
29899 44 13 38 61
23155 1624 1291 1423 2166
22911 886 707 778 1169
6884 439 376 405 584
56900 986 1078 1031 1591
677766 4 4 2 5
127003 1 2 2 2
57535 15 20 13 16
6301 2744 2808 2375 3527
1952 1491 1192 1335 2005
84722 123 55 98 164
343263 150 139 136 200
6272 18195 13856 17111 23574
5686 1368 1364 1089 1730
284612 435 479 471 626
127002 159 140 151 227
284613 265 262 286 457
57463 662 697 759 1050
83873 33 26 30 42
2773 926 814 749 1138
406974 0 1 0 2
2780 30 11 30 42
271 1144 1013 998 1394
2948 685 692 571 995
2946 413 453 393 544
2944 28 9 14 28
2949 6 3 6 12
2947 625 691 641 931
79574 0 0 0 0
1435 450 389 370 629
10768 1652 1623 1559 2454
85369 1363 1289 1244 1712
257 20 29 16 21
104355139 2 4 0 0
164153 2 2 2 4
388662 187 104 175 271
100873974 7 2 7 4
3749 842 827 774 1053
440600 0 10 2 8
64783 346 248 267 421
9122 27 16 35 40
10542 1085 1056 807 1345
101410535 1 3 2 7
84432 4 6 0 3
643160 0 0 0 0
440602 1 0 1 0
3744 0 0 0 1
3737 11 5 9 18
3738 0 1 0 0
963 0 4 0 2
55791 419 463 400 652
128338 776 663 661 1055
10390 649 576 530 811
79961 8 6 4 7
1117 0 0 0 0
149620 0 0 0 0
27159 0 0 0 0
128344 161 136 146 221
441897 0 0 0 0
5016 27 24 28 24
79084 1473 1414 1308 1836
515 4229 4780 3894 6326
128346 37 34 37 43
57413 5 3 2 0
140 0 0 0 0
100129269 49 35 33 53
5906 1802 1393 1353 2126
55924 2254 1968 1824 2804
100506343 56 42 41 79
101928718 30 17 24 24
11218 767 589 651 1025
3752 58 73 107 136
100874295 0 0 0 0
100873995 0 0 0 0
643355 7 8 6 8
55917 748 660 739 1124
100422976 0 0 0 1
7482 38 33 27 47
54879 482 484 430 605
829 2925 2627 2375 3816
4343 1206 958 1024 1316
389 4896 4427 3610 5735
333926 84 92 74 108
284467 1 0 1 1
100996702 15 1 8 10
6566 12522 8551 10005 16355
246182 1 7 7 11
100506392 33 9 19 40
100996251 12 19 9 21
9860 494 375 382 693
643441 0 0 0 0
260425 359 236 351 490
10745 935 797 843 1348
54665 271 283 259 386
100287722 14 13 15 12
26191 2 17 1 6
440603 6 1 4 5
10717 474 361 362 556
64858 99 72 83 143
101928846 3 8 2 6
204851 1285 1084 1140 1679
56944 289 186 164 272
148281 0 3 0 0
51592 1375 1102 1267 1811
10286 713 686 597 915
163259 96 109 93 178
270 0 1 0 1
4893 1392 1106 1160 1823
7812 11781 9997 10701 15884
80143 1294 935 1009 1613
6847 0 2 0 0
7252 0 0 0 0
10100 719 680 471 836
4803 92 90 78 86
81839 658 453 556 912
845 28114 34279 35739 53453
4808 0 5 1 0
101928995 1 0 0 0
101928977 0 0 0 3
55356 729 666 741 1034
126868 55 22 43 62
476 36388 25656 28431 43094
84852 26 25 24 54
101929023 0 0 0 0
965 445 432 425 669
100616384 0 0 0 0
3321 6373 3528 5242 7222
100302117 0 0 0 0
388667 0 0 0 0
914 0 1 0 0
5738 11806 7467 8521 13000
9398 37 16 11 34
101929099 0 2 1 0
8458 358 198 297 424
100126331 0 0 0 0
80263 185 181 152 254
79679 0 5 0 0
104355292 0 0 0 0
10905 2689 2117 2492 3707
100996263 0 5 0 0
54855 48 48 44 46
54834 266 308 278 428
10885 846 623 686 1023
200162 2 8 1 2
6913 0 2 0 0
10352 244 177 215 318
104472716 0 0 0 1
101929147 73 58 71 104
51179 0 0 0 0
100874270 0 0 0 0
3284 25 5 4 7
3283 0 0 0 2
128102 9 6 15 17
644242 40 31 42 55
90874 954 847 939 1356
26227 954 871 949 1292
3158 0 1 0 0
83998 1 2 0 0
343505 0 4 0 0
11085 0 0 0 0
4853 8636 4608 6134 8547
9554 1565 1580 1475 2255
105378954 19 13 8 17
728841 610 494 557 716
767846 32 17 17 22
100996724 2451 2462 2628 3677
101929796 140 96 123 195
101060684 876 686 798 1139
100189005 0 0 0 0
101954277 0 2 0 0
653505 13 14 7 12
728855 80 74 57 89
101954264 1 3 2 2
100189257 0 0 0 0
105369199 3 0 1 2
100996721 8 12 13 16
100126488 0 0 0 0
101929798 0 0 0 0
2210 3 5 3 4
100996318 2 6 3 4
337875 0 0 4 0
653820 75 36 58 138
653464 892 830 837 1219
100873165 9 13 9 13
647121 22 8 10 22
101929815 0 0 0 0
100189261 0 0 0 0
101954269 0 0 0 0
26863 1 1 0 1
791227 0 0 0 0
105369140 2 0 1 2
388692 4 5 3 8
100189142 2 0 0 0
100189326 0 0 0 0
199882 0 0 1 4
100189356 0 0 0 0
100132417 5 7 2 4
100996713 6 2 0 6
554282 56 30 47 89
100996712 477 363 385 583
101060524 0 1 0 0
730262 1 3 9 3
101954267 2 0 0 0
100189323 1 0 0 0
284565 649 531 546 719
100189127 0 0 0 1
728945 0 0 1 0
105371215 0 0 2 0
1818 0 1 0 0
645166 10 7 10 9
647135 656 599 651 939
728833 65 19 63 68
101929805 0 0 0 0
102724235 15 5 15 18
103091866 31 39 20 36
645142 0 1 0 2
101954266 0 1 1 3
7214 0 0 0 0
100288142 265 176 197 299
100126510 0 0 0 0
101060254 45 32 50 61
101929780 133 115 114 154
653519 315 303 340 420
5174 18 3 10 27
11126 38 41 34 44
27246 1149 1166 1081 1724
10623 548 487 469 690
200035 99 114 80 119
10401 753 675 652 1040
102466191 0 0 0 0
148741 5 3 0 0
8515 361 339 554 686
8799 460 412 452 645
114814 19 13 19 30
9939 1195 1123 1077 1708
128077 696 647 565 946
84265 617 630 501 838
284615 309 268 291 413
10628 4362 3811 3726 5362
148738 56 39 85 103
100189384 0 0 0 0
100189064 0 0 0 0
100188991 0 0 0 0
100189198 0 0 0 0
101954276 0 3 0 0
101928979 17 30 21 29
100132406 308 168 213 283
388677 227 189 237 374
170688 1222 970 1183 1849
105371242 0 0 0 0
102724558 0 0 0 1
100288805 8 15 9 7
105373468 13 11 18 16
149013 643 538 617 846
728989 34 48 36 75
100189334 0 0 0 0
101447996 2 0 1 0
644861 4 0 0 1
5565 1538 1659 1346 2143
171423 10820 8882 8960 13262
2330 119 58 77 135
9557 589 359 397 595
100289211 30 19 32 33
607 670 626 661 1010
51205 430 427 345 591
2702 2504 2259 3015 4548
2703 0 1 1 0
51463 458 403 349 599
100034743 19 21 19 25
100189140 0 0 0 0
26864 0 0 0 0
100189193 0 0 0 0
200030 258 236 199 330
101927468 0 1 0 0
100189056 0 0 0 0
100189307 0 0 0 0
100189174 0 0 0 0
100188998 0 0 0 0
100847044 0 0 0 1
101954273 0 0 1 0
102466225 0 0 0 0
26860 0 0 0 0
100132057 34 24 26 39
101954272 0 3 0 0
388685 55 57 46 97
644591 4 3 1 6
25832 697 491 603 866
100996763 287 177 261 391
440672 1480 1311 1544 2318
9659 7975 7651 8508 12984
400818 348 288 300 460
653513 782 1023 942 1594
100996761 24 4 28 34
101060226 533 401 457 714
653598 25 26 15 29
57234 92 78 70 108
644634 12 6 11 10
100189228 0 0 0 0
102723955 1 0 4 1
100189059 0 0 0 0
440689 19 31 46 50
2209 13 12 15 21
101927767 2 4 1 4
653604 3 7 0 2
8370 43 52 49 89
105371405 103 79 78 119
126961 4 6 15 14
8337 106 73 49 89
337873 18 19 12 29
723790 236 182 150 250
333932 5 2 5 7
554313 19 8 12 23
8349 2357 2396 1976 3244
8338 12 3 3 3
DOX_24_Ind2 FLUO_24_Ind2 DMSO_24_Ind2 DOX_24rec_Ind2 FLUO_24rec_Ind2
100287102 3 9 5 20 3
653635 155 220 177 292 185
102466751 5 10 0 5 3
100302278 10 0 0 0 0
645520 0 0 0 0 0
79501 0 0 0 0 0
729737 69 309 241 113 240
102725121 2 5 6 21 3
102723897 194 279 211 298 247
102465909 1 0 0 3 4
729759 0 3 0 0 0
100132287 60 141 93 72 112
105378947 8 0 0 0 3
101928626 0 0 0 0 0
102465432 1854 1472 1652 1594 1659
81399 0 0 0 2 0
100133331 51 148 88 77 116
100288069 4 15 18 10 18
400728 9 1 1 9 2
79854 35 15 16 27 12
643837 943 2306 1644 1280 2354
284593 3 1 2 2 0
100130417 144 38 17 20 23
148398 290 30 16 110 14
26155 3733 1650 1552 2869 1724
339451 307 162 157 145 177
84069 9 6 4 16 5
84808 7 3 0 1 0
57801 1522 792 608 558 572
9636 586 159 149 341 139
375790 10721 5827 5484 15847 5420
105369174 1 4 1 0 1
105378948 2 4 2 0 0
401934 1 0 0 0 0
54991 94 262 228 74 223
254099 0 0 0 0 0
406984 0 0 0 0 0
406983 0 0 0 0 0
554210 0 0 0 0 0
254173 1 0 0 0 0
8784 2 2 2 2 1
7293 0 0 0 2 0
51150 6087 4848 4500 7206 4984
126792 661 1161 1168 1245 1132
388581 17 6 10 20 9
118424 1421 918 744 1682 899
6339 486 226 107 186 108
116983 1661 1605 1184 1412 1269
102465434 0 0 0 0 0
126789 81 134 152 57 176
54973 962 1390 1101 1782 1153
102465435 0 0 0 0 0
80772 623 665 508 540 611
83756 154 33 29 55 21
1855 902 1172 967 1156 1055
102466740 0 0 0 0 0
54587 311 360 300 262 277
54998 1125 1429 1072 1522 1180
81669 4673 2362 1762 2244 2492
148413 275 233 228 303 255
55052 743 1018 904 1383 1043
441869 7 1 0 1 0
643965 0 0 0 0 1
102724312 5 23 21 5 17
64856 141 194 172 101 133
219293 11 22 18 12 21
83858 318 531 414 355 452
55210 318 910 745 426 860
339453 20 53 64 17 63
29101 1458 1741 1574 2641 1699
643988 319 751 626 104 562
142678 3163 2782 1964 2903 2135
8510 232 570 530 116 567
984 954 1195 915 1462 1109
728661 1535 2674 2191 2293 2659
8511 460 984 928 237 1091
728642 813 698 572 1039 680
9906 544 915 725 760 832
65220 733 868 673 1182 839
2782 3701 6853 6309 9000 7089
105378949 43 21 16 48 14
163688 29 8 5 27 3
339456 138 5 6 45 5
85452 13 32 10 1 6
2563 2 6 3 0 3
5590 143 836 685 179 747
100506504 56 69 31 24 42
199990 533 569 479 528 502
105378592 4 15 5 2 7
6497 3694 1423 1202 1800 1465
105378594 2 2 0 3 1
79906 306 144 97 156 119
100129534 143 51 50 53 40
11079 3918 2554 2351 4162 2687
5192 143 352 267 592 305
9651 11 10 8 6 9
55229 709 1248 1023 1091 1255
388585 5 0 0 0 1
115110 1 0 1 0 0
8764 0 0 0 0 1
100996583 0 1 0 0 0
127281 175 507 414 99 480
79258 3 2 0 0 0
100287898 20 17 12 22 13
140625 1 0 0 0 0
440556 469 897 525 148 725
63976 150 1182 1052 152 1400
100422968 1 0 0 0 1
27237 65 72 37 110 68
1953 301 397 345 174 381
693135 0 0 0 0 0
127262 1676 970 826 1435 1001
49856 153 207 170 394 219
7161 104 21 15 21 12
57212 1085 836 652 683 807
148870 49 75 41 38 56
388588 9 45 38 15 44
57470 2501 2379 2096 3055 2391
9731 1696 746 565 1251 798
1677 62 55 57 59 60
339448 62 251 199 209 241
100133612 15 7 4 20 0
728716 0 0 0 0 0
284661 0 0 0 0 0
55966 7 0 0 1 0
100616489 0 0 0 0 0
100616421 0 0 0 0 0
261734 123 178 127 91 160
8514 251 63 51 68 36
26038 43 11 4 30 2
6146 2070 2717 2293 2762 2910
102724450 13 43 29 6 33
388591 653 4828 3407 171 4540
23463 1688 4613 4248 4466 4396
148645 22 2 5 4 3
390992 1 0 0 0 2
387509 972 227 235 528 232
11332 1113 269 302 401 347
54626 67 0 0 62 1
83715 68 14 8 322 9
100422975 0 0 0 0 0
8718 30 110 101 76 99
57449 423 633 472 233 485
79707 1368 947 785 1203 911
80835 26 1 0 4 2
3104 324 310 282 380 334
9903 3791 2854 2925 3916 3014
148479 1131 608 654 892 849
90326 80 136 152 170 183
55735 926 1494 1373 1794 1646
100505887 0 0 0 0 0
23261 814 998 859 1467 1057
9341 2042 2139 1783 4137 2330
8863 81 455 338 87 384
10911 0 0 0 3 0
3604 14 46 77 12 87
11315 2896 3347 3231 4839 3666
54206 1027 535 416 2303 546
102724539 1 0 1 0 0
50651 27 44 32 134 38
473 2847 3376 2933 4428 3592
102724552 29 1 1 7 0
2023 26605 37995 29280 23455 31981
102465436 0 0 0 0 0
100505975 43 12 4 23 4
765 1 0 0 5 0
155184 0 0 0 0 0
6518 5 1 1 13 0
80045 114 125 107 34 118
407040 3 0 3 11 3
9563 1885 2471 1998 2592 2350
80176 521 50 45 249 42
100506022 0 9 13 1 16
84275 207 148 147 161 164
199953 1146 1103 988 1021 1107
5293 498 146 120 441 126
644997 1 0 0 0 0
101929074 3 9 2 8 9
22883 3279 4097 3636 3874 4659
56998 1154 1140 1106 900 1101
84328 562 902 685 922 895
64802 1185 1005 1008 1427 1243
100847055 0 1 1 0 0
105376719 1 0 0 0 1
116362 90 22 13 39 10
10277 1743 2340 1971 1986 2614
23095 8361 6871 5926 9570 7196
100422970 0 0 0 0 0
5226 7711 2827 2444 6445 3075
100526739 2 1 2 1 0
378708 52 53 31 48 44
1325 10 5 7 6 4
1676 800 899 781 1648 1030
5195 305 575 583 500 546
54897 5289 4183 3767 2125 4505
148345 46 17 9 41 20
23435 2536 3549 3307 3634 4244
10747 98 111 71 71 56
6723 979 674 602 641 649
5394 1003 1688 1272 1431 1679
2475 3540 4286 3400 4538 4004
100873935 0 2 0 2 0
10218 0 10 22 2 17
29914 905 786 749 735 723
57540 71 3 2 37 0
101929181 1 0 0 2 0
26232 1023 340 292 2128 233
93611 1494 851 647 1000 813
26270 61 49 55 110 51
10459 428 376 302 348 382
374946 30 2 2 25 4
57085 490 284 336 755 345
284498 1 1 1 4 0
102724659 1 0 0 0 0
4524 1727 2238 2020 1584 2397
1185 1067 1281 1030 1394 1304
100379251 2 4 9 10 9
4878 51844 49858 66452 27122 64896
4879 5654 13030 16922 1380 13236
26829 61 2 0 19 1
90231 3085 1802 1665 2704 1856
5351 4386 7336 6100 2964 6889
9927 7428 15180 14140 10565 15303
60672 177 326 382 256 303
102466982 1 0 0 0 0
943 1 1 0 4 0
102465836 0 0 0 0 0
7133 19 4 2 48 6
100616438 0 0 0 0 0
55187 1905 2555 1993 3743 2401
677885 1 2 1 2 5
9249 1523 257 265 1672 344
102466722 2 0 0 0 1
343066 0 0 0 0 0
126767 0 0 0 1 0
93190 0 0 0 0 0
390999 0 0 5 1 1
65121 0 2 9 1 1
440560 0 0 3 0 1
343069 4 4 3 10 6
65122 0 0 4 0 0
400735 0 0 0 0 0
343071 0 0 1 0 0
441871 0 1 1 7 0
440561 0 0 0 0 2
653606 0 0 0 0 0
102724742 0 0 1 0 0
101929983 0 0 0 0 0
649330 2 3 2 0 1
441873 0 0 1 1 0
649324 0 0 0 0 0
440563 6 2 1 2 0
645354 0 0 1 2 1
645359 0 0 2 0 0
101060301 0 0 4 1 0
343070 0 0 2 0 2
400736 0 0 0 1 0
391003 0 0 1 0 0
343068 0 1 1 2 5
391002 0 0 1 3 0
645382 0 0 0 0 0
653619 0 0 0 0 0
729528 0 0 4 1 1
645414 0 0 2 0 0
391004 0 0 0 0 0
645425 0 2 0 1 1
126755 23 61 91 62 76
10630 1283 3007 3092 658 3712
7799 3120 1482 1228 2023 1591
23254 233 184 198 231 182
200197 22 11 3 47 7
55092 340 285 267 678 356
727684 2 2 1 3 0
114827 1 4 2 5 3
101927417 0 3 2 1 0
79180 153 267 221 183 203
11330 0 0 1 0 0
63036 0 1 0 1 0
51032 4 6 6 17 4
842 392 798 546 859 680
23341 1736 1036 944 1555 1444
79814 37 10 11 14 13
84301 164 123 87 206 141
6248 144 105 108 159 108
23207 2316 2653 2309 4405 2572
284723 20 43 39 48 28
388595 3 0 0 0 0
54751 1072 2733 2455 3007 2731
440567 1149 1544 1371 797 1808
729614 783 220 172 675 185
23013 2110 1564 1210 2646 1699
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127255 1 3 3 5 5
100526835 14 58 68 8 84
8790 43 325 301 218 418
51086 48 175 126 33 233
105378804 8 27 16 13 43
105378803 1 17 6 0 13
127254 14 0 4 35 2
101927320 0 0 0 0 0
1429 1272 846 789 3282 1015
127253 647 591 551 1054 757
431707 9 5 0 8 0
204962 256 161 212 674 307
34 3513 3614 3310 3715 3817
1744 159 266 254 326 302
5876 377 1468 1262 1803 1464
692085 8 16 0 8 12
26805 3 14 9 13 28
26804 1 16 8 8 9
4438 14 15 2 13 4
127247 1 0 0 0 0
101927342 1 0 0 0 1
256435 253 354 348 397 403
81849 12 52 51 33 53
102466995 0 0 0 0 0
10026 799 1407 1277 685 1599
26289 16 8 6 16 9
26009 413 1130 1008 1346 1374
23032 2442 2134 1885 2750 2367
374986 1105 624 538 1208 694
374987 117 23 19 127 20
91624 2880 18797 18492 9080 20519
8880 689 3042 2686 3603 3191
11080 3650 1616 1505 2622 1976
54810 86 31 26 30 36
149047 0 0 0 0 0
5737 2 1 3 1 5
10964 0 0 0 1 0
10561 0 0 0 1 1
64123 20 213 279 64 236
101927412 0 0 0 0 0
23266 4720 4892 4036 5353 5152
101927434 0 0 0 0 0
101927498 2 3 3 14 2
101927587 0 1 1 0 0
101927560 0 0 0 1 0
79739 280 311 260 519 399
5567 2316 2606 2279 3325 2779
148418 7 20 11 7 16
391051 1 1 0 3 2
58511 0 0 0 1 0
80135 1107 1131 1080 1530 1189
2787 1112 3359 2980 3178 3561
100505741 32 39 25 87 28
1486 325 558 553 1839 704
103695366 4 3 1 10 8
439927 3 3 0 4 1
117178 1726 1387 1212 2762 1662
23566 229 697 585 454 719
255231 17 2 2 5 1
55283 834 53 44 880 56
126820 1576 162 126 1705 156
100616481 1 0 0 0 1
84144 743 654 524 897 755
148423 840 497 431 803 548
8915 197 243 243 485 284
646626 11 6 4 55 3
23576 2226 1145 1058 3745 1121
3491 1025 1543 2065 2953 1784
54680 168 421 364 622 471
255631 12 15 12 25 11
57489 687 674 593 1081 712
102465842 0 1 0 1 0
9635 10 1 0 269 2
1179 3 0 0 5 0
22802 6 0 0 3 0
9629 2 0 0 4 0
105378828 523 28 28 370 36
51100 3092 6564 6355 23530 7529
9403 2982 1947 1959 6559 2295
9653 3817 3312 3305 9330 3977
339524 10 4 3 17 6
101927844 0 0 0 0 0
105378832 0 0 0 0 0
8543 1623 3205 2824 5052 3496
100505768 0 0 0 0 1
101927891 17 2 3 4 3
5586 1216 3518 3131 2508 3904
2959 2261 409 326 1248 486
56267 250 289 223 342 342
494115 606 1147 1110 1584 1328
2635 13 36 39 114 33
2633 2 11 16 5 12
2634 49 93 82 437 113
388646 6 0 0 2 1
115361 1 11 9 10 15
115362 4 0 3 0 1
729930 0 0 0 1 0
163351 2 1 1 19 0
400759 0 0 0 1 0
23507 933 400 503 3604 460
84230 1175 435 427 2868 560
400761 38 22 25 116 26
55144 317 618 575 306 667
105378846 0 0 0 1 0
492303 56 16 8 15 18
284695 427 1060 851 993 1010
343472 0 0 0 0 0
84146 1847 1918 1680 2778 2240
164045 8 70 49 20 53
8317 333 344 350 218 443
7049 336 509 395 134 454
676 0 1 0 3 0
253152 9 41 37 20 61
646817 42 67 90 76 94
284697 16 16 6 9 26
23285 45 89 85 76 132
388649 1 4 6 1 4
11146 186 462 380 307 588
79871 409 398 357 606 536
2672 63 10 5 76 3
7813 1361 786 700 1143 769
6125 5995 9256 7511 9597 9311
388650 332 635 527 244 636
6083 2 2 3 1 2
26782 0 4 9 4 3
22823 578 722 643 689 854
50999 3781 3581 3626 3204 4459
343099 293 189 157 164 360
100131564 179 277 170 519 205
1810 2815 1344 1156 2821 1595
54874 1236 804 688 1872 840
100126520 0 0 0 0 0
8412 86 35 39 390 51
100129046 3 0 0 2 1
100126348 7 0 0 1 0
100189133 19 0 2 3 0
30836 2217 1511 1427 2509 1544
2730 464 275 249 773 418
24 3 1 0 10 1
9411 881 1183 872 889 1160
5825 998 2987 2579 2533 3176
2152 80 225 181 62 194
101928079 13 20 9 25 15
100616321 0 0 0 0 0
126969 72 69 58 146 46
1266 4590 2861 2698 5847 3078
105378862 193 21 17 36 27
729970 352 172 169 117 170
199857 76 109 132 189 152
101928098 2 0 0 0 0
148534 4813 6067 5613 3813 7429
100527978 62 69 58 50 82
101928118 14 16 11 3 3
25950 139 338 331 225 423
440594 0 0 0 0 0
100996635 0 0 0 1 0
102723661 0 0 1 0 0
101928241 0 0 0 0 0
58155 634 992 861 1111 1121
1806 20 20 10 70 15
100873932 0 0 0 2 0
100873933 0 0 0 0 0
400765 0 0 0 1 0
100616452 0 0 0 0 0
406928 0 0 0 0 0
729987 0 0 0 0 0
51375 741 559 497 397 639
163404 275 0 1 37 0
100129620 0 0 0 0 0
9890 6 32 28 39 33
101928270 0 0 0 0 0
54873 2 1 8 2 8
391059 184 301 296 701 417
178 1760 13984 10635 4066 13116
23443 411 525 524 876 683
64645 1246 1159 1161 1712 1379
163786 41 296 237 87 340
54482 101 333 268 365 340
127495 55 1485 1028 588 1298
1629 1031 1838 1632 2436 2013
100506007 69 57 45 29 39
8634 1016 1306 1225 1399 1378
693138 0 0 0 0 0
8556 384 208 208 444 215
54112 28 12 4 16 7
100128787 0 0 0 0 0
7412 42 3717 3155 63 4775
2135 823 966 915 529 1277
148867 534 334 350 763 372
51611 180 703 684 666 859
102606465 31 232 218 231 222
101928334 1 0 0 0 0
101928370 280 167 189 313 216
1901 925 5509 5821 1629 6167
104355286 0 0 0 0 0
118427 4 6 8 4 6
94236 0 0 0 1 0
1301 257 229 187 190 171
101928436 6 0 0 0 1
55599 449 899 528 721 684
280 457 783 485 434 553
648740 8 37 24 13 17
279 16 20 16 12 8
276 12 22 15 13 10
277 2 3 3 4 0
278 9 13 4 4 11
100129138 0 2 3 2 1
101928476 0 0 0 0 0
55170 795 899 883 1202 1045
22854 1 4 0 5 0
10451 32 0 5 11 0
102465839 0 0 0 0 0
100873946 0 0 0 0 0
29957 556 1628 1413 1776 1720
148545 0 0 0 0 0
653149 0 0 0 0 0
284611 632 682 589 1300 891
113802 160 32 31 128 45
55119 980 1400 1169 1686 1451
163479 1 1 0 3 0
6814 952 1052 994 1977 1226
254268 0 0 0 0 0
642864 0 0 0 0 0
29899 12 120 61 38 217
23155 738 1035 927 903 1273
22911 2433 720 670 1517 863
6884 803 353 383 642 439
56900 604 1600 1485 1146 1611
677766 13 9 6 6 3
127003 14 0 2 4 0
57535 162 13 11 50 8
6301 3346 2872 2535 5888 3006
1952 6349 907 839 1481 1015
84722 12 93 83 2 155
343263 21 25 30 62 21
6272 5925 6637 6937 4760 8925
5686 1958 1258 1248 2142 1431
284612 1749 1280 1404 2112 1413
127002 104 179 131 155 151
284613 175 270 261 705 303
57463 185 517 503 632 673
83873 22 39 45 17 44
2773 1247 1360 1135 3209 1288
406974 1 1 1 2 2
2780 12 19 23 26 20
271 630 918 728 777 830
2948 886 1521 1247 823 1521
2946 399 563 402 213 532
2944 19 30 39 11 32
2949 7 20 13 4 19
2947 1149 933 778 535 975
79574 0 1 0 0 1
1435 2990 578 528 4163 718
10768 3363 2600 2255 3499 2659
85369 1212 1452 1221 1700 1392
257 6 20 19 33 18
104355139 1 1 0 0 1
164153 1 2 0 0 0
388662 57 337 282 610 314
100873974 204 6 6 38 5
3749 1915 1048 849 1819 946
440600 25 16 6 13 2
64783 1011 442 369 499 472
9122 19 22 24 29 20
10542 613 1475 1276 1477 1519
101410535 12 16 8 16 15
84432 0 1 0 0 1
643160 7 25 23 4 36
440602 8 12 17 1 20
3744 0 0 0 0 0
3737 128 10 10 29 16
3738 89 1 0 4 6
963 0 2 0 3 0
55791 836 611 516 770 689
128338 396 972 938 928 1181
10390 930 570 471 1257 582
79961 81 4 6 40 6
1117 91 0 1 75 1
149620 0 0 0 0 0
27159 0 0 0 1 0
128344 261 393 274 468 349
441897 2 0 0 0 0
5016 158 31 20 28 22
79084 1792 1663 1587 3090 1789
515 4325 7447 7010 5963 7938
128346 42 59 47 40 41
57413 1 0 2 2 1
140 1 0 0 0 0
100129269 57 24 22 54 12
5906 1523 1869 1577 2470 1993
55924 3648 1492 1259 2575 1566
100506343 90 31 25 53 26
101928718 55 10 7 9 4
11218 500 876 816 623 919
3752 53 23 16 12 12
100874295 0 0 0 0 0
100873995 0 0 0 0 0
643355 6 1 0 2 4
55917 846 766 700 1519 928
100422976 1 0 0 1 0
7482 81 29 16 38 20
54879 438 341 290 553 462
829 2977 2950 2825 7030 3358
4343 1364 898 672 1220 891
389 4636 4330 4387 11554 4458
333926 371 113 104 411 101
284467 27 0 0 7 2
100996702 2 5 3 4 1
6566 10556 23194 23185 11625 26453
246182 6 27 16 7 14
100506392 18 54 27 32 49
100996251 37 19 9 16 17
9860 250 629 593 639 648
643441 1 0 0 0 0
260425 521 430 336 261 445
10745 785 1330 1286 1091 1559
54665 593 434 407 631 662
100287722 32 5 2 9 6
26191 21 2 2 8 1
440603 3 3 9 6 3
10717 229 407 388 507 447
64858 130 165 148 69 172
101928846 98 3 4 6 3
204851 3782 2000 1580 3045 2240
56944 159 442 388 130 434
148281 6 1 0 8 0
51592 1440 2219 1880 2830 2411
10286 722 983 766 1797 976
163259 194 92 84 379 95
270 0 0 0 2 0
4893 865 1579 1350 2624 1666
7812 20098 19918 16783 23475 21427
80143 1161 1916 1523 3185 2150
6847 0 0 0 5 0
7252 0 0 1 2 0
10100 834 2486 1964 1287 2223
4803 72 3 5 388 6
81839 448 737 590 487 948
845 3724 5194 6153 2156 6033
4808 429 4 0 281 3
101928995 3 1 0 5 0
101928977 6 0 0 1 0
55356 228 475 413 349 479
126868 1 25 14 6 18
476 31936 51127 50688 53025 54950
84852 49 65 85 103 90
101929023 3 2 0 2 1
965 227 444 455 519 526
100616384 0 0 0 0 0
3321 4453 4382 3969 3997 5078
100302117 0 0 0 0 0
388667 0 0 0 2 0
914 0 0 0 0 0
5738 6209 8490 7736 3344 10131
9398 28 18 16 6 19
101929099 4 3 0 1 2
8458 934 301 271 430 359
100126331 0 1 0 0 0
80263 186 276 221 302 277
79679 17 1 0 7 0
104355292 0 0 0 0 0
10905 2731 2613 2466 3355 2864
100996263 0 0 0 0 0
54855 596 74 62 838 64
54834 1076 369 322 754 435
10885 730 801 749 999 906
200162 2 1 1 9 2
6913 1 0 2 2 2
10352 381 419 389 405 422
104472716 0 1 0 0 0
101929147 94 87 96 94 107
51179 1 0 0 2 0
100874270 0 0 0 0 0
3284 2 1 2 1 0
3283 4 0 0 0 0
128102 3 1 0 30 0
644242 14 1 4 36 5
90874 510 505 437 680 552
26227 1135 1160 1201 234 1416
3158 2 0 0 5 5
83998 0 0 0 1 1
343505 0 0 0 1 0
11085 0 0 0 2 0
4853 9303 5522 5075 8022 6827
9554 728 1646 1549 2100 1922
105378954 52 15 9 55 16
728841 331 485 426 463 478
767846 9 10 7 8 8
100996724 1495 4159 3066 1709 4206
101929796 82 78 60 93 85
101060684 710 952 654 833 955
100189005 2 1 0 0 0
101954277 3 0 0 1 0
653505 25 12 8 46 13
728855 128 71 77 109 88
101954264 0 2 0 0 0
100189257 0 0 1 0 0
105369199 1 3 2 0 1
100996721 6 7 1 3 2
100126488 0 0 0 0 0
101929798 0 0 0 0 0
2210 32 8 4 30 1
100996318 6 1 6 4 3
337875 8 3 1 6 0
653820 230 56 50 39 99
653464 1013 667 613 1213 783
100873165 3 5 4 5 5
647121 3 9 12 15 11
101929815 2 0 0 3 2
100189261 0 0 0 0 0
101954269 0 0 0 0 0
26863 4 1 2 0 0
791227 0 0 0 0 0
105369140 2 0 0 1 2
388692 4 1 2 0 9
100189142 0 1 0 0 1
100189326 0 0 0 0 0
199882 0 0 0 0 0
100189356 0 0 0 0 0
100132417 47 3 4 65 3
100996713 12 7 4 8 4
554282 168 33 51 22 66
100996712 564 297 241 505 302
101060524 2 0 0 1 0
730262 6 1 3 14 8
101954267 2 0 2 3 0
100189323 2 2 0 1 0
284565 281 521 411 330 553
100189127 0 0 0 0 1
728945 2 3 3 5 0
105371215 1 0 0 0 0
1818 0 0 0 0 0
645166 0 0 0 3 2
647135 684 519 420 904 565
728833 182 54 40 24 69
101929805 0 0 0 0 0
102724235 6 13 4 3 12
103091866 54 21 15 26 35
645142 2 1 0 1 3
101954266 4 0 2 1 0
7214 0 0 0 0 0
100288142 167 216 153 142 217
100126510 0 0 0 0 0
101060254 6 40 37 14 30
101929780 140 141 98 123 121
653519 342 335 248 399 377
5174 18 18 12 17 16
11126 5 45 36 24 38
27246 1369 1976 1930 2397 2237
10623 1178 756 614 850 777
200035 84 149 138 146 132
10401 718 788 674 1049 879
102466191 0 0 0 0 0
148741 6 1 1 9 0
8515 24 55 55 11 84
8799 349 539 502 765 653
114814 11 37 28 7 34
9939 2967 2347 1960 4543 2374
128077 318 953 946 1211 1081
84265 959 794 673 587 766
284615 144 220 172 110 274
10628 226 406 504 82 796
148738 4 109 101 9 88
100189384 0 0 1 0 0
100189064 1 0 0 0 0
100188991 0 0 0 0 0
100189198 0 0 0 0 0
101954276 0 0 1 0 0
101928979 4 20 23 13 16
100132406 231 296 242 269 355
388677 325 479 459 577 526
170688 651 4934 3828 1987 5209
105371242 0 0 0 1 0
102724558 0 1 0 0 2
100288805 3 10 5 14 7
105373468 7 3 5 6 8
149013 447 635 489 677 631
728989 16 37 33 15 42
100189334 0 0 0 0 0
101447996 0 0 0 0 0
644861 0 1 0 0 0
5565 5174 2851 2506 4734 3596
171423 4787 4604 4248 6060 4568
2330 217 134 131 194 133
9557 552 553 447 325 578
100289211 7 11 15 6 8
607 480 1159 971 898 1268
51205 235 482 380 242 473
2702 15 1269 1202 34 1599
2703 5 0 0 4 0
51463 417 432 350 484 422
100034743 12 5 11 17 9
100189140 0 0 0 0 0
26864 1 0 0 1 0
100189193 0 0 0 0 0
200030 159 279 205 303 261
101927468 1 0 0 0 0
100189056 0 0 0 0 0
100189307 0 0 0 0 0
100189174 0 0 0 0 0
100188998 0 0 0 0 0
100847044 0 1 0 0 3
101954273 2 0 0 0 0
102466225 0 0 0 0 0
26860 0 0 0 0 0
100132057 9 28 25 7 45
101954272 0 0 0 2 1
388685 120 36 32 70 49
644591 2 4 0 10 5
25832 657 907 744 672 976
100996763 381 623 533 727 684
440672 985 5872 5053 2467 6896
9659 6349 18149 14742 7860 18678
400818 249 378 279 298 364
653513 913 2238 1810 1068 2439
100996761 70 31 13 73 36
101060226 448 650 496 529 665
653598 33 16 23 75 29
57234 133 71 72 143 82
644634 12 7 8 2 6
100189228 0 0 0 0 0
102723955 0 1 0 0 0
100189059 0 0 0 0 0
440689 209 68 54 264 60
2209 242 37 31 235 36
101927767 18 10 11 41 15
653604 5 1 2 12 2
8370 194 67 45 507 63
105371405 568 135 98 1649 134
126961 120 20 10 327 19
8337 758 78 62 1243 63
337873 106 19 15 282 19
723790 2227 234 206 3052 185
333932 51 2 3 64 9
554313 64 18 15 125 13
8349 11298 3877 3571 10972 3644
8338 50 5 6 45 6
DMSO_24rec_Ind2 DOX_144rec_Ind2 FLUO_144rec_Ind2 DMSO_144rec_Ind2
100287102 5 6 3 9
653635 192 191 253 264
102466751 4 2 3 6
100302278 0 0 0 0
645520 0 0 0 0
79501 0 1 0 0
729737 217 186 234 310
102725121 9 4 9 10
102723897 247 251 288 335
102465909 1 0 2 0
729759 1 0 0 0
100132287 141 122 154 175
105378947 0 3 2 3
101928626 0 0 0 0
102465432 1353 3004 1989 2559
81399 1 0 0 0
100133331 117 151 136 174
100288069 22 13 24 29
400728 3 1 2 2
79854 5 35 18 35
643837 2092 1381 2192 2280
284593 1 0 0 0
100130417 14 17 2 20
148398 20 18 11 24
26155 1808 1795 1913 2141
339451 183 225 250 266
84069 1 5 6 4
84808 5 3 3 1
57801 672 523 580 723
9636 151 103 123 138
375790 5734 10430 5799 7364
105369174 1 0 0 1
105378948 1 4 0 2
401934 0 0 0 0
54991 282 180 185 240
254099 0 0 0 0
406984 0 0 0 0
406983 0 0 0 0
554210 0 0 0 0
254173 0 0 0 0
8784 2 0 2 2
7293 0 1 1 1
51150 5294 5886 5322 5836
126792 1275 1147 1168 1379
388581 22 17 13 31
118424 953 735 952 886
6339 113 119 173 223
116983 1277 1328 1194 1270
102465434 0 0 0 0
126789 159 122 179 154
54973 1388 1206 1689 1722
102465435 0 0 0 0
80772 684 545 617 743
83756 23 46 57 47
1855 1195 1096 1357 1477
102466740 0 0 0 0
54587 257 280 262 264
54998 1486 767 1228 1278
81669 2211 2980 3093 3412
148413 244 236 280 254
55052 1104 668 1079 1031
441869 0 0 0 3
643965 0 0 1 0
102724312 16 3 5 13
64856 148 74 100 123
219293 17 17 23 27
83858 470 531 550 619
55210 850 703 914 1002
339453 71 38 81 84
29101 1989 1113 1706 1635
643988 721 368 497 515
142678 2238 2409 2735 2848
8510 656 328 770 850
984 1214 1004 1286 1397
728661 2689 2436 3109 3301
8511 1286 619 1301 1369
728642 771 754 841 959
9906 830 738 1026 1107
65220 877 776 987 1047
2782 7577 5454 5842 6139
105378949 16 28 14 14
163688 1 5 1 9
339456 4 2 3 6
85452 6 0 2 6
2563 0 2 0 6
5590 818 486 638 670
100506504 50 48 39 43
199990 558 315 556 511
105378592 10 14 13 6
6497 1476 1423 1489 1577
105378594 2 3 0 4
79906 96 92 163 204
100129534 49 28 50 50
11079 2847 2550 2757 2931
5192 328 268 254 276
9651 4 12 12 11
55229 1303 1194 1330 1408
388585 0 0 0 0
115110 0 0 0 0
8764 0 0 0 0
100996583 0 0 1 0
127281 485 276 397 420
79258 0 3 4 2
100287898 5 24 11 18
140625 0 0 0 0
440556 580 251 920 925
63976 1381 789 1921 1734
100422968 0 0 0 1
27237 60 35 36 48
1953 327 500 317 388
693135 0 0 0 0
127262 979 721 980 942
49856 221 212 257 269
7161 16 22 10 13
57212 833 621 774 782
148870 73 58 61 65
388588 43 16 32 48
57470 2496 1896 2674 2852
9731 839 888 899 987
1677 68 52 79 89
339448 237 246 278 301
100133612 1 7 9 10
728716 0 0 0 0
284661 0 0 0 0
55966 0 1 0 0
100616489 0 0 0 0
100616421 0 0 0 0
261734 157 115 151 171
8514 56 35 56 56
26038 0 27 11 7
6146 2924 1459 2218 2301
102724450 16 24 24 35
388591 4517 3744 5772 6017
23463 4705 4318 3631 3690
148645 2 2 2 2
390992 0 0 0 0
387509 291 213 142 154
11332 389 170 187 255
54626 1 4 0 0
83715 5 6 4 3
100422975 0 0 0 0
8718 88 262 112 155
57449 431 1008 485 520
79707 1043 867 1034 1110
80835 2 3 1 0
3104 369 386 429 464
9903 3173 4265 3027 3456
148479 820 566 827 828
90326 186 150 206 212
55735 1630 1341 1566 1585
100505887 0 0 0 0
23261 1090 479 841 790
9341 2402 1597 2067 1930
8863 369 187 441 526
10911 1 4 1 2
3604 71 75 48 68
11315 3993 2281 3277 3246
54206 444 956 379 391
102724539 1 0 0 0
50651 47 59 49 86
473 3744 3508 4063 4351
102724552 0 0 2 3
2023 30943 12154 20185 26767
102465436 2 1 0 0
100505975 5 6 7 14
765 0 1 0 0
155184 0 0 0 0
6518 0 4 0 1
80045 112 66 63 79
407040 3 6 1 1
9563 2458 2530 2524 3059
80176 44 70 28 44
100506022 14 2 7 16
84275 164 84 132 130
199953 1177 1136 1208 1365
5293 156 126 114 135
644997 0 0 0 0
101929074 6 3 3 6
22883 4658 6372 5722 6591
56998 1188 671 765 737
84328 914 540 762 720
64802 1307 1087 1285 1249
100847055 0 1 3 4
105376719 0 1 0 0
116362 18 4 11 8
10277 2632 1983 2729 2821
23095 7490 8987 8173 8762
100422970 0 0 0 0
5226 3119 2539 2534 2572
100526739 1 0 0 0
378708 51 28 32 33
1325 16 11 15 8
1676 1100 888 1104 1033
5195 677 552 725 751
54897 5155 5305 5774 6507
148345 12 16 21 15
23435 3913 3236 3765 3746
10747 53 76 60 85
6723 724 382 562 546
5394 1731 1439 1980 2011
2475 4671 3979 4881 4831
100873935 0 1 1 0
10218 15 7 6 7
29914 841 758 823 866
57540 3 0 1 1
101929181 0 0 0 0
26232 244 295 180 220
93611 808 781 945 1059
26270 65 69 84 68
10459 459 172 286 303
374946 4 3 1 0
57085 393 240 307 332
284498 1 0 1 0
102724659 0 0 0 0
4524 2581 1856 2857 3223
1185 1282 1735 1781 2102
100379251 16 23 9 6
4878 68924 98668 40299 43035
4879 14760 35028 5319 6469
26829 0 7 3 4
90231 2064 2017 1955 2214
5351 7121 8940 4888 6314
9927 16177 21500 15466 16953
60672 348 611 347 379
102466982 0 0 0 0
943 2 0 0 0
102465836 0 0 0 0
7133 3 0 2 2
100616438 0 0 0 0
55187 2771 3740 3768 4356
677885 7 1 6 3
9249 415 457 599 664
102466722 0 0 1 0
343066 0 0 0 0
126767 0 1 0 0
93190 0 0 0 0
390999 9 1 7 5
65121 2 2 3 5
440560 2 0 0 3
343069 4 1 2 3
65122 1 2 6 8
400735 0 0 0 1
343071 1 0 0 0
441871 2 1 2 2
440561 0 0 0 0
653606 0 0 0 0
102724742 1 0 0 3
101929983 2 2 0 1
649330 1 1 0 1
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84144 719 720 907 887
148423 581 431 584 608
8915 292 233 288 272
646626 2 2 4 6
23576 1233 896 833 862
3491 1879 3365 1960 1824
54680 470 415 380 465
255631 19 26 8 7
57489 721 590 611 645
102465842 0 0 2 0
9635 1 6 1 0
1179 0 4 1 0
22802 0 2 1 0
9629 0 1 0 1
105378828 33 21 15 28
51100 7622 5878 6658 6016
9403 2367 1576 1777 1603
9653 4097 3514 3602 3480
339524 4 7 5 2
101927844 0 0 0 0
105378832 0 0 0 0
8543 3427 3443 3525 3336
100505768 0 0 2 0
101927891 5 4 2 1
5586 4098 4697 4206 4245
2959 454 342 420 384
56267 318 275 288 323
494115 1348 827 1171 1069
2635 37 25 38 27
2633 13 7 6 12
2634 94 52 52 50
388646 1 0 1 0
115361 14 3 8 2
115362 0 1 2 1
729930 2 0 3 0
163351 0 3 0 1
400759 0 0 1 0
23507 458 914 346 394
84230 522 660 412 408
400761 34 17 27 17
55144 741 909 784 798
105378846 0 0 1 0
492303 13 5 18 20
284695 961 914 1205 1156
343472 0 1 0 0
84146 2228 2189 2584 2355
164045 44 44 81 66
8317 497 114 278 256
7049 556 148 542 577
676 0 3 0 2
253152 61 15 29 36
646817 55 55 71 83
284697 23 21 38 19
23285 131 100 149 185
388649 1 2 3 7
11146 544 338 486 484
79871 599 440 654 604
2672 5 3 5 6
7813 875 844 806 817
6125 10125 5071 7866 8238
388650 661 441 508 529
6083 2 4 2 0
26782 5 5 4 6
22823 861 589 821 773
50999 4613 3641 3803 3828
343099 415 84 136 161
100131564 230 441 318 422
1810 1613 1103 1530 1531
54874 901 813 999 1007
100126520 0 0 0 0
8412 65 60 43 64
100129046 2 0 0 1
100126348 0 0 0 0
100189133 2 0 1 2
30836 1819 1638 1795 1787
2730 392 261 359 387
24 0 8 0 0
9411 1111 761 1031 1253
5825 3055 2821 3671 3780
2152 167 176 248 283
101928079 17 11 9 14
100616321 0 0 0 0
126969 59 40 64 63
1266 3159 1891 1903 1901
105378862 25 21 24 26
729970 160 58 114 105
199857 133 90 96 80
101928098 0 3 2 0
148534 7516 6304 6843 6232
100527978 78 66 72 81
101928118 4 5 12 13
25950 413 304 366 339
440594 0 0 0 0
100996635 0 0 1 0
102723661 0 1 0 0
101928241 0 3 0 0
58155 1167 1034 1061 1130
1806 13 13 2 11
100873932 0 0 0 0
100873933 0 0 0 0
400765 0 0 0 0
100616452 0 0 0 0
406928 0 0 0 0
729987 0 1 0 0
51375 631 340 520 487
163404 1 4 0 0
100129620 0 0 0 0
9890 34 7 25 16
101928270 0 0 0 0
54873 10 1 3 6
391059 365 394 294 371
178 12680 9316 10712 10890
23443 667 683 604 578
64645 1419 2008 1596 1579
163786 390 109 243 219
54482 371 417 453 434
127495 1304 648 1354 1463
1629 1993 1599 2059 1956
100506007 40 25 43 44
8634 1480 1036 1390 1288
693138 2 1 0 0
8556 238 304 224 248
54112 5 3 1 3
100128787 0 2 0 0
7412 4343 4416 2974 3155
2135 1198 785 985 909
148867 388 555 407 498
51611 906 707 751 804
102606465 247 257 254 262
101928334 0 1 0 0
101928370 217 307 172 135
1901 6161 7975 6597 6252
104355286 0 0 0 0
118427 5 2 2 4
94236 0 0 0 0
1301 147 100 123 128
101928436 0 0 4 0
55599 544 744 942 914
280 416 553 668 774
648740 21 13 31 43
279 6 16 17 15
276 10 17 14 16
277 1 2 2 4
278 8 4 10 12
100129138 2 1 3 2
101928476 0 2 0 0
55170 1099 850 932 963
22854 0 4 3 0
10451 8 5 1 0
102465839 0 0 0 0
100873946 0 0 0 0
29957 1790 1123 1248 1362
148545 0 2 0 0
653149 0 0 0 0
284611 843 749 479 414
113802 38 35 24 26
55119 1529 1352 1634 1670
163479 1 1 0 2
6814 1165 1483 1373 1434
254268 0 0 0 0
642864 0 1 0 0
29899 227 27 85 95
23155 1275 1288 1350 1393
22911 866 1096 796 857
6884 394 300 373 335
56900 1796 1464 1755 1652
677766 6 7 7 10
127003 0 2 2 0
57535 12 26 15 8
6301 3397 3006 5447 5365
1952 1127 1508 1592 1863
84722 220 1 47 68
343263 22 19 9 16
6272 9228 14634 9145 9795
5686 1553 1057 1374 1296
284612 1570 2519 1248 1315
127002 129 187 154 127
284613 300 286 343 333
57463 723 828 881 831
83873 28 15 31 38
2773 1318 1001 1123 1123
406974 0 1 1 2
2780 14 25 35 48
271 814 707 828 944
2948 1630 714 1196 1208
2946 538 232 443 389
2944 41 8 25 29
2949 21 2 13 14
2947 942 388 772 767
79574 1 0 0 0
1435 759 1248 1059 1104
10768 2769 2178 2987 3031
85369 1428 1510 1541 1718
257 27 38 24 29
104355139 0 0 0 0
164153 0 0 1 1
388662 291 475 348 480
100873974 6 9 0 8
3749 989 1448 899 924
440600 4 2 5 2
64783 523 434 516 559
9122 14 15 32 30
10542 1629 850 1265 1199
101410535 10 7 19 13
84432 1 1 4 10
643160 25 38 39 39
440602 16 40 64 35
3744 0 0 0 0
3737 16 34 11 15
3738 2 4 0 2
963 0 0 0 1
55791 744 477 740 783
128338 1228 1199 1443 1544
10390 489 868 803 801
79961 5 4 5 8
1117 0 3 0 0
149620 0 0 0 0
27159 0 0 0 0
128344 309 146 196 185
441897 0 0 0 0
5016 23 22 34 33
79084 2002 1520 1672 1675
515 8423 5332 7938 7772
128346 58 52 77 105
57413 0 0 0 0
140 0 0 0 0
100129269 26 24 10 14
5906 1946 1233 1541 1560
55924 1599 1808 1255 1364
100506343 37 18 18 25
101928718 7 5 5 8
11218 956 762 1024 1024
3752 28 22 36 38
100874295 0 0 0 0
100873995 0 0 0 0
643355 1 1 1 2
55917 929 1073 954 873
100422976 0 0 2 1
7482 24 33 21 31
54879 476 452 495 536
829 3445 3006 2927 2719
4343 823 961 928 957
389 4895 3947 3689 3594
333926 126 101 88 89
284467 1 0 1 1
100996702 3 0 5 6
6566 25236 46540 26636 30356
246182 10 23 8 26
100506392 38 59 41 58
100996251 23 28 25 41
9860 658 839 812 835
643441 0 0 0 0
260425 530 330 437 473
10745 1573 989 1215 1152
54665 633 572 676 733
100287722 4 10 10 11
26191 0 1 0 0
440603 4 6 3 4
10717 457 495 407 477
64858 220 49 137 144
101928846 3 6 7 2
204851 2152 2359 2768 2907
56944 432 239 315 289
148281 0 0 0 0
51592 2614 1920 2478 2607
10286 1044 627 818 837
163259 116 107 110 114
270 1 1 0 0
4893 1787 1367 1342 1366
7812 21123 15038 18846 18495
80143 2137 1815 2169 2185
6847 0 6 0 0
7252 2 2 0 1
10100 2053 1981 1509 1708
4803 12 31 5 10
81839 918 521 815 757
845 6528 13879 7586 7969
4808 0 17 3 1
101928995 0 1 1 1
101928977 0 0 1 1
55356 449 692 523 596
126868 16 7 9 14
476 57623 89822 51078 59362
84852 66 69 68 85
101929023 3 3 1 2
965 552 399 442 423
100616384 1 0 0 2
3321 5468 5886 4717 5328
100302117 0 0 0 0
388667 0 0 0 0
914 0 0 0 0
5738 10265 8721 9458 10259
9398 29 15 14 26
101929099 4 0 2 0
8458 433 106 191 188
100126331 0 0 0 0
80263 304 232 266 296
79679 0 3 1 2
104355292 0 0 0 0
10905 2949 2538 3058 3068
100996263 0 0 0 0
54855 72 117 40 46
54834 445 458 457 481
10885 969 992 947 1093
200162 2 10 5 2
6913 2 3 1 0
10352 427 251 380 411
104472716 1 1 2 0
101929147 104 53 113 107
51179 0 0 0 0
100874270 0 0 0 0
3284 5 1 0 4
3283 0 5 0 0
128102 0 1 0 2
644242 6 11 4 6
90874 618 585 628 688
26227 2039 631 4413 4490
3158 1 4 3 0
83998 0 1 1 0
343505 0 0 0 0
11085 0 0 0 0
4853 7853 11288 9420 10779
9554 1849 1536 1926 1854
105378954 19 6 16 18
728841 538 520 561 618
767846 5 13 12 23
100996724 4155 3481 4745 4616
101929796 88 88 90 96
101060684 1106 960 1069 1283
100189005 0 0 0 0
101954277 0 0 3 0
653505 14 4 12 8
728855 96 40 108 102
101954264 0 0 1 2
100189257 0 0 2 0
105369199 2 0 5 1
100996721 3 0 2 7
100126488 0 0 0 0
101929798 0 0 0 0
2210 4 3 0 7
100996318 0 1 3 5
337875 1 2 1 2
653820 124 17 40 44
653464 829 731 783 739
100873165 2 7 4 5
647121 18 8 7 2
101929815 1 0 2 3
100189261 0 0 0 0
101954269 0 0 0 0
26863 1 2 2 0
791227 0 0 0 0
105369140 0 0 2 2
388692 4 1 0 2
100189142 0 0 0 0
100189326 0 0 0 0
199882 0 0 0 0
100189356 0 0 0 0
100132417 4 4 5 3
100996713 7 1 3 4
554282 102 4 25 40
100996712 316 273 286 282
101060524 0 0 0 0
730262 4 3 3 7
101954267 0 0 1 1
100189323 0 0 0 0
284565 548 423 437 543
100189127 1 0 0 0
728945 2 0 0 1
105371215 0 0 0 0
1818 0 1 0 0
645166 1 1 3 2
647135 637 563 509 534
728833 86 13 23 25
101929805 0 0 0 0
102724235 5 4 9 18
103091866 27 11 32 15
645142 0 1 0 1
101954266 0 1 1 2
7214 0 2 0 1
100288142 242 195 301 326
100126510 0 0 0 0
101060254 32 26 31 36
101929780 142 126 136 174
653519 391 336 396 400
5174 12 21 13 22
11126 36 60 30 42
27246 2382 2077 2065 1774
10623 817 600 914 852
200035 149 124 136 144
10401 866 780 825 839
102466191 0 0 0 2
148741 4 1 1 0
8515 77 71 94 94
8799 736 508 619 572
114814 27 19 41 39
9939 2434 1734 1828 1837
128077 1139 1028 1035 1020
84265 798 483 633 592
284615 234 228 170 142
10628 740 810 1483 2187
148738 76 58 40 40
100189384 0 0 0 0
100189064 0 1 0 0
100188991 0 0 0 0
100189198 0 0 0 0
101954276 0 0 0 0
101928979 12 22 18 13
100132406 415 362 447 519
388677 587 444 467 407
170688 5144 3353 6124 6118
105371242 0 0 1 0
102724558 1 0 0 2
100288805 9 11 5 4
105373468 9 4 17 10
149013 625 636 801 829
728989 57 51 70 65
100189334 0 0 0 0
101447996 0 0 0 0
644861 0 1 1 0
5565 3564 4534 4350 4088
171423 4521 4907 4129 4719
2330 150 128 140 121
9557 636 402 409 509
100289211 6 17 3 3
607 1359 973 1284 1190
51205 470 466 580 601
2702 1584 1572 1064 1345
2703 0 1 0 0
51463 470 444 513 533
100034743 18 29 8 20
100189140 0 0 0 0
26864 0 0 0 0
100189193 0 0 0 0
200030 261 207 275 300
101927468 2 0 4 0
100189056 0 0 0 0
100189307 0 0 0 0
100189174 0 0 0 0
100188998 0 0 0 0
100847044 3 1 0 2
101954273 0 0 1 0
102466225 0 0 0 0
26860 0 0 0 0
100132057 43 23 44 54
101954272 0 0 0 0
388685 50 38 41 34
644591 9 2 4 4
25832 1203 995 1211 1463
100996763 764 596 646 698
440672 6873 4277 7858 7691
9659 19372 14184 20060 20230
400818 397 325 337 376
653513 2554 1364 2084 1938
100996761 26 44 23 35
101060226 736 576 760 885
653598 29 18 9 11
57234 77 68 99 109
644634 5 2 6 7
100189228 0 0 0 0
102723955 0 0 0 0
100189059 0 0 0 0
440689 33 32 46 45
2209 35 22 22 20
101927767 14 6 12 9
653604 0 0 2 1
8370 66 68 86 79
105371405 108 151 163 172
126961 14 15 17 20
8337 81 75 69 77
337873 19 20 10 20
723790 178 159 143 184
333932 5 9 4 6
554313 14 14 18 26
8349 3572 4359 3749 3645
8338 6 3 4 4
DOX_24_Ind3 FLUO_24_Ind3 DMSO_24_Ind3 DOX_24rec_Ind3 FLUO_24rec_Ind3
100287102 2 0 0 0 0
653635 86 127 161 220 147
102466751 5 2 1 5 1
100302278 7 0 1 4 0
645520 1 0 0 2 0
79501 0 0 0 0 0
729737 215 308 486 126 259
102725121 2 0 1 2 0
102723897 144 161 225 282 151
102465909 0 0 0 2 1
729759 0 0 0 0 0
100132287 106 160 242 141 131
105378947 1 1 1 5 2
101928626 0 0 0 0 0
102465432 3819 2211 2125 1952 1742
81399 0 0 1 0 0
100133331 71 143 240 115 116
100288069 6 18 24 6 12
400728 2 0 3 9 4
79854 11 20 13 67 19
643837 932 2159 2156 797 2023
284593 2 2 1 3 0
100130417 40 16 19 61 12
148398 70 22 20 306 17
26155 2327 1821 1873 2878 1500
339451 288 229 309 364 245
84069 9 3 8 30 2
84808 9 6 2 14 4
57801 1001 1056 1146 930 741
9636 185 166 206 348 163
375790 17898 11030 14667 8683 8196
105369174 6 2 5 8 0
105378948 10 19 2 111 3
401934 10 0 0 58 0
54991 169 263 344 144 193
254099 0 0 0 0 0
406984 0 0 0 0 0
406983 0 0 0 0 0
554210 1 0 0 0 0
254173 1 0 0 4 0
8784 8 0 1 18 1
7293 7 1 0 3 0
51150 8193 4896 5227 5097 4721
126792 390 1215 1443 910 1145
388581 20 13 19 40 8
118424 401 957 1032 1348 797
6339 287 191 228 342 120
116983 2190 1428 1987 1932 1143
102465434 0 0 0 0 0
126789 66 170 202 125 151
54973 949 1245 1442 1099 1140
102465435 0 0 0 0 1
80772 87 591 645 439 426
83756 51 34 58 68 24
1855 533 1088 1270 668 952
102466740 0 1 0 0 0
54587 190 207 236 155 183
54998 1032 1312 1351 1347 931
81669 6106 2505 3112 3415 2631
148413 112 275 299 406 261
55052 557 1044 1113 1071 838
441869 21 0 0 50 0
643965 0 0 0 0 1
102724312 12 10 10 8 11
64856 236 193 273 107 193
219293 40 27 25 28 8
83858 580 456 551 854 370
55210 505 805 1022 457 677
339453 16 70 78 15 52
29101 791 1922 1899 1374 1538
643988 585 450 669 160 366
142678 2124 2101 2670 3295 1846
8510 906 632 683 189 658
984 554 1093 1140 1696 849
728661 1184 3087 3450 1649 2849
8511 1910 1265 1278 357 1381
728642 766 1015 1150 1795 924
9906 636 1525 1764 874 1443
65220 545 904 975 787 621
2782 4215 7592 7479 3069 6231
105378949 18 11 9 55 9
163688 12 5 4 24 6
339456 65 7 7 102 5
85452 22 6 9 17 10
2563 6 0 1 8 2
5590 361 854 900 183 712
100506504 20 33 43 22 35
199990 659 655 650 465 531
105378592 8 6 9 14 5
6497 1159 1777 2005 3838 1419
105378594 9 9 0 27 2
79906 112 79 166 181 85
100129534 33 31 38 33 24
11079 2260 2715 2846 2212 2697
5192 171 290 318 260 249
9651 7 3 19 24 8
55229 431 1061 1252 624 1049
388585 2 0 0 6 0
115110 8 0 0 3 0
8764 15 0 0 5 0
100996583 2 0 1 2 0
127281 220 427 570 158 338
79258 12 0 2 4 3
100287898 27 29 17 33 17
140625 0 0 0 0 0
440556 243 552 734 126 493
63976 386 1020 1250 90 870
100422968 0 0 0 0 0
27237 68 76 106 126 57
1953 1684 1075 1421 479 747
693135 0 0 0 0 0
127262 1234 1061 1141 2260 890
49856 66 226 291 253 205
7161 74 11 14 158 9
57212 893 800 956 689 739
148870 35 51 55 32 61
388588 17 55 76 14 61
57470 1353 1896 2125 2311 1864
9731 784 746 730 1142 678
1677 33 80 95 54 61
339448 111 264 249 208 215
100133612 5 4 7 10 6
728716 0 0 0 0 0
284661 0 0 0 0 0
55966 1 0 0 2 0
100616489 0 0 0 0 0
100616421 0 0 0 0 0
261734 113 197 247 177 120
8514 241 52 73 319 38
26038 47 8 16 78 1
6146 5014 3648 3404 1575 2468
102724450 29 9 28 11 10
388591 4384 4248 5322 586 3760
23463 2049 3735 4068 1205 3710
148645 12 2 3 6 3
390992 3 0 0 4 0
387509 591 223 309 470 233
11332 993 465 567 655 398
54626 16 0 1 185 0
83715 39 4 6 884 7
100422975 0 0 0 0 0
8718 179 179 253 63 173
57449 929 854 1100 455 734
79707 588 797 921 850 704
80835 46 1 3 26 1
3104 145 303 431 432 275
9903 2053 3701 4087 4761 3649
148479 840 870 978 1248 806
90326 118 153 164 158 153
55735 653 1516 1648 841 1442
100505887 2 0 0 0 0
23261 893 950 896 628 706
9341 2567 2276 2188 1806 1728
8863 161 256 295 169 320
10911 1 9 1 4 4
3604 188 188 240 32 89
11315 3275 3674 3632 2015 2844
54206 656 808 870 4131 627
102724539 0 1 0 1 0
50651 15 61 39 45 41
473 2473 4107 4605 3307 3201
102724552 42 1 3 15 1
2023 30349 18260 22027 8671 23058
102465436 0 0 0 1 0
100505975 18 3 3 42 2
765 2 0 0 5 0
155184 0 0 0 23 0
6518 22 1 2 125 0
80045 121 112 110 503 106
407040 2 3 1 14 1
9563 1242 2205 2476 1061 1976
80176 238 114 147 1277 90
100506022 4 8 7 5 7
84275 135 144 136 111 138
199953 293 965 1171 830 911
5293 232 162 192 312 140
644997 3 0 0 0 0
101929074 25 5 8 12 8
22883 3792 5483 6629 2674 5101
56998 999 1042 1065 537 850
84328 277 808 757 504 745
64802 695 945 853 792 883
100847055 0 2 2 3 3
105376719 14 1 0 6 0
116362 82 36 40 89 41
10277 1751 3050 3068 1319 2744
23095 2286 6377 6502 5065 6063
100422970 0 0 0 1 0
5226 4185 2950 3301 8973 2746
100526739 0 4 0 1 0
378708 6 44 39 48 43
1325 7 5 9 14 4
1676 455 1002 977 602 863
5195 79 598 611 330 573
54897 2084 4499 5175 1332 3975
148345 2 17 17 35 16
23435 2321 3813 3809 4476 3658
10747 58 70 100 156 55
6723 988 770 981 536 612
5394 570 1548 1602 1121 1267
2475 2024 4042 4650 2451 3442
100873935 1 0 1 6 0
10218 14 23 6 1 22
29914 314 705 739 527 731
57540 49 1 1 2632 0
101929181 5 0 0 7 0
26232 696 285 342 790 188
93611 874 804 875 544 818
26270 43 44 42 42 37
10459 249 419 414 371 332
374946 19 4 3 66 3
57085 118 331 407 577 373
284498 16 0 0 3 0
102724659 0 0 0 0 0
4524 846 2249 2554 962 2094
1185 777 1040 1310 1295 1159
100379251 37 12 24 9 19
4878 221383 102513 97515 34344 140892
4879 65732 31208 27181 2024 29710
26829 33 2 1 62 4
90231 1797 1941 2191 1582 1856
5351 12729 6797 8198 2892 7082
9927 12696 14499 14866 5417 14361
60672 158 398 439 228 301
102466982 0 0 0 0 0
943 2 1 2 3 1
102465836 0 0 0 0 0
7133 10 1 4 54 1
100616438 0 0 0 0 0
55187 922 2473 2744 1133 2093
677885 2 3 9 0 0
9249 463 227 274 668 223
102466722 0 0 0 0 0
343066 0 0 0 1 0
126767 0 0 0 2 0
93190 0 0 0 0 0
390999 1 0 0 7 0
65121 0 1 0 7 0
440560 0 0 0 1 0
343069 10 5 6 5 7
65122 0 0 0 6 0
400735 0 0 0 1 0
343071 0 0 0 0 0
441871 0 0 0 10 0
440561 0 0 0 2 0
653606 0 0 0 0 0
102724742 0 0 0 0 0
101929983 0 0 0 0 0
649330 3 0 0 1 3
441873 0 0 0 3 0
649324 0 0 0 0 0
440563 1 2 3 7 2
645354 0 0 0 14 0
645359 0 0 0 2 0
101060301 2 0 1 0 0
343070 0 0 0 2 0
400736 0 0 0 0 0
391003 0 0 0 0 0
343068 1 0 0 0 0
391002 0 0 0 0 0
645382 0 0 0 0 0
653619 0 0 0 3 0
729528 0 1 0 1 0
645414 0 0 0 1 0
391004 0 0 0 1 0
645425 3 0 0 3 0
126755 15 5 13 11 19
10630 1999 2368 2340 341 2580
7799 659 1452 1501 4667 1343
23254 124 213 224 127 207
200197 20 8 12 5 7
55092 249 249 275 148 270
727684 0 1 1 3 0
114827 0 2 2 4 1
101927417 0 1 3 1 0
79180 143 188 235 315 158
11330 4 0 0 7 0
63036 3 5 0 1 0
51032 4 6 14 7 3
842 496 683 737 329 653
23341 632 1421 1559 1452 1531
79814 35 16 19 41 17
84301 45 125 127 154 98
6248 8 110 126 109 110
23207 1411 2976 3065 2569 2150
284723 99 27 42 47 41
388595 7 0 0 2 0
54751 1042 2586 2681 1033 2068
440567 1909 1650 1480 527 1231
729614 120 184 190 691 206
23013 955 1736 2044 2916 1462
7709 173 616 655 609 530
101928566 1 0 0 0 1
149563 1 0 0 0 0
27129 18340 53470 53007 3497 49597
1187 96 91 106 279 66
1188 229 280 325 79 194
348487 17 39 39 13 57
1969 1836 4325 4461 12820 3499
128272 39 38 37 85 34
101927546 3 18 11 7 8
79363 22 143 166 126 119
54455 161 908 978 548 921
26099 2824 3452 3454 3128 2597
374955 16 2 0 9 0
55707 60 303 327 450 260
114819 62 127 162 192 132
26871 3 1 0 6 0
100126512 4 0 2 3 0
100126517 0 3 2 0 5
102724970 6 15 29 6 16
100133301 1 0 0 0 0
7195 0 0 0 1 0
55672 285 409 564 412 358
84809 142 214 307 267 235
11209 80 34 63 60 44
26869 3 0 0 4 0
729574 1 0 0 0 0
100126533 0 0 0 0 0
284729 1 0 3 2 1
100189045 0 0 0 0 0
102723383 0 0 0 0 0
6060 2 0 1 12 1
11223 42 43 33 295 36
100500876 0 0 0 0 0
100189298 0 0 0 0 0
7193 1 0 3 1 3
791226 0 0 0 0 0
9696 163 175 276 489 171
26870 8 0 0 12 0
4237 331 386 401 177 374
23400 1254 1312 1596 633 1043
6390 1885 2052 2050 1022 2003
11240 56 31 27 48 11
29943 7 7 8 5 6
51702 1 0 0 12 0
100616188 0 0 0 0 0
23569 0 0 0 0 0
353238 5 0 0 1 0
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2787 1274 3889 3564 1116 2942
100505741 72 19 28 56 19
1486 503 419 428 521 420
103695366 0 4 4 6 4
439927 20 2 2 6 1
117178 893 1583 1422 1255 1453
23566 138 526 420 295 512
255231 9 6 7 7 4
55283 86 9 6 373 12
126820 154 252 209 1295 239
100616481 0 0 1 2 0
84144 303 837 822 635 788
148423 616 621 584 1084 563
8915 117 261 236 644 228
646626 3 3 1 34 0
23576 3937 1253 1275 940 1092
3491 1193 5150 4917 6858 2934
54680 119 508 480 258 449
255631 13 7 8 28 4
57489 759 800 741 546 674
102465842 0 1 0 0 0
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1179 1 1 0 2 0
22802 5 1 0 122 1
9629 1 0 1 2 0
105378828 22 31 32 165 28
51100 1909 6376 5930 4232 5602
9403 3653 2159 1935 2053 2103
9653 1967 4154 3913 3011 4258
339524 33 6 4 23 4
101927844 0 0 0 1 0
105378832 0 0 0 0 0
8543 1682 3542 3264 1995 3399
100505768 3 2 1 1 0
101927891 13 1 0 18 3
5586 1089 4647 4142 2006 4406
2959 936 469 372 4752 432
56267 251 217 219 97 180
494115 226 1188 1134 942 1023
2635 6 33 54 33 49
2633 14 11 10 5 15
2634 81 223 185 159 155
388646 3 0 2 2 1
115361 10 23 31 6 12
115362 0 1 1 16 2
729930 0 2 2 0 1
163351 2 3 2 5 2
400759 1 0 0 2 0
23507 539 1029 1016 885 958
84230 259 643 685 689 555
400761 18 28 37 32 27
55144 190 1078 1105 289 928
105378846 2 0 0 1 0
492303 77 20 15 42 26
284695 626 1058 1101 639 893
343472 16 0 0 8 0
84146 841 1897 1853 4186 1908
164045 55 82 85 21 82
8317 250 351 372 137 188
7049 547 313 362 192 334
676 3 0 5 6 2
253152 23 84 80 35 93
646817 26 61 81 47 66
284697 6 43 42 15 26
23285 23 168 174 89 157
388649 5 3 0 4 4
11146 182 449 467 171 490
79871 181 509 428 334 455
2672 639 0 6 100 5
7813 437 979 934 664 862
6125 12424 11444 10001 5151 8396
388650 231 569 573 157 642
6083 1 1 2 1 3
26782 0 8 6 2 1
22823 360 840 804 1050 713
50999 5153 5020 4436 2664 5250
343099 130 355 369 226 185
100131564 382 289 334 302 208
1810 1233 1377 1315 2701 1286
54874 819 1028 951 1672 976
100126520 0 1 0 0 0
8412 74 63 65 177 52
100129046 7 3 1 18 0
100126348 0 0 1 0 0
100189133 4 2 1 4 7
30836 724 1414 1228 2261 1332
2730 168 399 373 783 391
24 17 0 1 11 0
9411 699 1172 1117 1879 940
5825 1437 3199 2990 1084 3118
2152 476 253 231 303 229
101928079 14 12 27 17 12
100616321 0 0 0 0 0
126969 50 77 76 75 70
1266 3932 3825 3395 4307 3185
105378862 32 37 33 94 20
729970 163 179 161 200 147
199857 43 141 156 54 137
101928098 1 1 0 0 3
148534 6953 6750 5941 1902 7942
100527978 41 82 72 21 95
101928118 14 7 8 11 5
25950 152 405 354 159 432
440594 0 0 0 0 0
100996635 1 0 0 0 0
102723661 0 0 0 1 0
101928241 0 0 0 0 0
58155 442 930 852 1224 999
1806 17 14 17 39 9
100873932 0 0 0 0 0
100873933 0 0 0 1 0
400765 1 0 0 3 0
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406928 0 0 0 0 0
729987 0 1 1 7 0
51375 716 580 565 221 492
163404 116 1 0 42 0
100129620 6 0 0 1 0
9890 17 10 19 31 24
101928270 0 0 0 0 0
54873 3 9 8 6 2
391059 222 323 367 138 347
178 8231 10855 9969 1274 12877
23443 552 681 635 623 643
64645 701 1336 1382 1262 1518
163786 67 287 305 87 198
54482 166 371 404 238 367
127495 317 1013 920 271 1130
1629 1533 1987 1884 1325 1868
100506007 67 51 43 82 31
8634 1599 1464 1310 676 1319
693138 0 0 3 0 0
8556 573 236 199 379 210
54112 28 4 4 17 1
100128787 2 0 0 0 0
7412 527 5424 5150 102 5083
2135 1517 1131 1127 498 1157
148867 957 393 505 757 414
51611 120 787 674 181 680
102606465 84 212 202 61 208
101928334 0 0 0 0 0
101928370 140 210 190 146 114
1901 617 6514 6207 1470 6236
104355286 0 0 0 0 0
118427 4 0 8 9 2
94236 0 0 0 1 0
1301 344 92 117 322 99
101928436 18 2 0 3 0
55599 685 813 889 582 764
280 867 611 715 402 462
648740 18 24 30 10 25
279 42 20 22 16 16
276 21 17 24 13 6
277 6 6 1 1 1
278 18 19 9 5 5
100129138 0 4 0 1 3
101928476 0 0 0 1 0
55170 535 954 955 745 786
22854 26 0 1 63 1
10451 60 25 22 17 9
102465839 0 0 0 0 0
100873946 2 0 0 0 0
29957 1279 1717 1635 1112 1380
148545 1 0 0 0 0
653149 2 0 0 2 0
284611 523 645 604 339 379
113802 45 34 29 147 33
55119 559 1321 1309 1812 1328
163479 25 2 2 20 0
6814 695 1117 1056 1369 1193
254268 5 0 0 3 0
642864 1 0 0 0 0
29899 43 192 166 47 81
23155 1112 1514 1524 614 1453
22911 854 1008 918 2948 877
6884 927 485 441 1077 439
56900 896 1645 1488 869 1735
677766 23 1 3 22 3
127003 7 1 3 25 1
57535 104 5 1 188 11
6301 1234 2595 2487 2875 2328
1952 2224 1532 1966 1213 1341
84722 46 179 210 38 85
343263 43 64 72 46 48
6272 11929 11917 12591 3074 10547
5686 800 1592 1441 1650 1308
284612 939 852 906 734 918
127002 95 132 166 353 123
284613 57 247 323 514 297
57463 187 779 826 262 957
83873 40 30 54 24 34
2773 254 1172 1080 1647 1105
406974 2 2 2 1 2
2780 16 12 25 20 29
271 771 932 1016 902 817
2948 1556 1174 1163 526 946
2946 911 585 605 272 395
2944 46 41 29 12 18
2949 22 12 18 5 8
2947 2379 938 794 2856 832
79574 0 0 0 1 0
1435 343 603 691 2326 628
10768 3278 2142 2217 2468 1940
85369 803 1398 1501 1151 1287
257 6 23 32 18 11
104355139 0 0 0 1 0
164153 2 2 1 3 1
388662 58 159 195 109 203
100873974 14 4 1 40 6
3749 473 769 844 607 956
440600 21 5 14 16 1
64783 400 392 435 1236 341
9122 24 14 28 126 20
10542 934 1388 1195 803 1156
101410535 18 8 12 23 13
84432 3 1 2 3 0
643160 4 2 3 4 3
440602 7 2 3 2 5
3744 1 0 0 0 0
3737 124 2 4 118 7
3738 94 2 1 65 1
963 1 0 0 10 0
55791 280 619 553 1181 699
128338 461 1046 1000 338 1174
10390 318 489 542 876 523
79961 11 5 2 82 5
1117 15 0 1 152 0
149620 4 0 0 2 0
27159 2 0 1 1 0
128344 227 271 262 146 228
441897 3 0 0 7 0
5016 89 10 18 225 17
79084 3613 1855 1772 1822 1600
515 7877 7155 6746 3056 7264
128346 128 50 44 90 58
57413 0 0 0 2 0
140 0 0 0 2 0
100129269 29 24 20 21 11
5906 833 1822 1682 1288 1595
55924 983 2177 2095 2103 1917
100506343 28 45 25 62 24
101928718 45 7 5 28 11
11218 129 895 878 789 808
3752 35 44 54 22 49
100874295 1 0 0 0 0
100873995 0 0 0 0 0
643355 19 2 2 8 12
55917 164 872 833 952 821
100422976 0 0 0 0 0
7482 44 21 31 70 23
54879 249 501 478 408 522
829 3497 4087 3724 3451 3392
4343 1173 927 1229 900 781
389 4586 4524 4266 3628 3499
333926 326 103 117 247 105
284467 90 0 3 21 1
100996702 9 2 7 5 3
6566 14396 20124 20153 15117 23985
246182 14 18 20 41 8
100506392 22 25 43 27 27
100996251 14 17 19 14 10
9860 84 586 619 432 652
643441 1 0 0 0 0
260425 516 497 555 321 502
10745 423 1324 1485 581 1333
54665 667 519 496 711 541
100287722 23 4 12 26 7
26191 29 2 1 22 1
440603 8 0 1 15 1
10717 253 423 517 446 445
64858 320 187 201 276 124
101928846 8 6 10 34 8
204851 2283 2372 2417 4453 2013
56944 360 273 302 70 256
148281 36 0 0 83 0
51592 818 2185 2249 2021 1974
10286 209 934 819 952 840
163259 117 124 120 485 122
270 10 0 0 3 0
4893 1446 2008 1948 3264 1712
7812 30863 20400 18917 14836 18210
80143 1492 2002 1813 1180 1778
6847 0 0 0 0 0
7252 3 1 2 2 1
10100 1894 1485 1382 535 1979
4803 3 14 7 106 4
81839 179 841 810 498 661
845 41469 20445 19538 9857 30346
4808 55 4 2 580 4
101928995 1 0 0 1 2
101928977 1 0 1 2 1
55356 247 490 604 193 516
126868 7 48 29 6 20
476 35349 55994 61931 46393 51474
84852 11 70 47 86 44
101929023 2 2 1 4 0
965 202 496 433 197 578
100616384 0 2 0 0 0
3321 10480 8575 9314 3454 7339
100302117 0 0 0 0 0
388667 0 0 0 0 0
914 2 0 0 3 0
5738 13196 8740 9881 2977 8478
9398 59 14 19 18 24
101929099 6 5 1 0 3
8458 286 450 466 476 319
100126331 0 0 0 0 0
80263 206 179 198 260 214
79679 18 0 0 11 0
104355292 0 0 0 0 0
10905 1623 3026 3029 1186 2939
100996263 1 0 0 0 0
54855 334 41 54 1417 36
54834 359 389 375 708 358
10885 839 939 896 900 941
200162 3 3 2 9 2
6913 33 2 3 42 0
10352 319 468 370 233 384
104472716 0 0 0 0 0
101929147 85 132 134 239 108
51179 0 2 0 1 0
100874270 0 0 0 0 0
3284 8 2 0 0 1
3283 6 0 0 2 0
128102 1 3 4 15 2
644242 4 14 10 53 7
90874 89 767 765 806 716
26227 1224 966 1112 523 922
3158 1 0 0 60 0
83998 0 0 1 5 0
343505 5 0 0 2 0
11085 2 0 0 1 0
4853 8080 8605 9614 7012 7391
9554 1521 2029 1914 927 1756
105378954 53 23 13 36 4
728841 760 643 765 502 538
767846 53 17 20 21 11
100996724 2587 4114 4649 882 3659
101929796 222 190 224 144 148
101060684 2190 1431 1808 1156 1190
100189005 8 1 0 14 0
101954277 2 3 0 30 0
653505 26 25 8 26 6
728855 168 105 112 62 83
101954264 1 3 1 1 0
100189257 0 0 1 0 0
105369199 8 6 2 2 1
100996721 14 9 11 6 6
100126488 0 0 0 1 0
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2210 17 7 12 82 8
100996318 14 2 1 14 6
337875 17 3 1 28 0
653820 295 109 107 111 72
653464 425 848 795 655 621
100873165 4 7 10 2 2
647121 17 10 23 4 9
101929815 1 1 0 1 2
100189261 0 0 0 0 0
101954269 0 0 0 0 0
26863 3 0 1 3 1
791227 0 0 0 0 0
105369140 9 0 5 6 0
388692 17 4 7 3 6
100189142 0 0 0 0 1
100189326 0 0 0 0 0
199882 13 2 4 4 1
100189356 0 0 0 0 0
100132417 49 8 9 76 9
100996713 14 7 1 22 4
554282 308 85 71 106 38
100996712 339 400 356 511 286
101060524 8 0 0 1 0
730262 14 6 6 11 1
101954267 0 0 2 8 0
100189323 1 0 0 1 0
284565 627 562 792 415 594
100189127 1 0 2 1 0
728945 2 0 0 2 1
105371215 10 0 0 0 0
1818 2 0 0 0 0
645166 9 3 7 3 7
647135 467 620 645 673 409
728833 339 92 70 102 34
101929805 4 0 0 2 0
102724235 18 21 18 7 13
103091866 131 40 43 28 23
645142 3 1 0 1 0
101954266 1 3 0 7 2
7214 0 0 2 7 2
100288142 313 236 338 230 221
100126510 0 0 0 0 0
101060254 27 69 67 12 61
101929780 245 166 158 168 141
653519 212 332 345 275 375
5174 19 11 15 49 13
11126 22 40 44 44 35
27246 772 2576 2184 1631 2054
10623 406 625 647 777 651
200035 202 117 91 183 105
10401 554 843 887 793 881
102466191 0 0 0 0 0
148741 13 0 2 14 3
8515 361 340 310 101 244
8799 103 652 617 442 629
114814 16 37 25 28 20
9939 2392 2186 2170 2658 1692
128077 361 954 1065 390 851
84265 874 731 729 494 689
284615 111 191 212 69 197
10628 297 3195 3137 1262 3392
148738 19 45 42 2 49
100189384 0 0 0 0 0
100189064 2 0 0 0 0
100188991 2 0 0 0 0
100189198 1 0 0 1 0
101954276 0 0 0 1 0
101928979 13 20 25 15 27
100132406 550 431 492 310 335
388677 552 584 588 289 469
170688 387 4087 3673 1068 3252
105371242 4 1 0 0 0
102724558 0 2 2 2 2
100288805 9 5 18 10 9
105373468 6 8 24 11 6
149013 879 743 856 634 616
728989 42 83 91 9 48
100189334 0 0 0 0 0
101447996 0 0 0 0 0
644861 0 0 0 3 0
5565 4997 3121 2587 4568 3226
171423 7730 6600 6579 4850 5758
2330 179 142 111 154 119
9557 869 554 664 266 482
100289211 4 36 22 32 19
607 531 1083 1219 848 1016
51205 308 600 607 241 490
2702 56 1223 1273 31 877
2703 7 0 0 3 0
51463 228 475 404 341 463
100034743 21 16 9 33 14
100189140 0 0 0 0 0
26864 0 0 0 1 0
100189193 0 0 0 0 0
200030 302 238 292 240 238
101927468 0 2 2 0 1
100189056 0 1 0 0 0
100189307 0 0 0 0 0
100189174 0 0 0 1 0
100188998 0 0 0 0 0
100847044 0 0 1 0 0
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102466225 0 0 0 0 0
26860 0 0 0 2 0
100132057 18 46 39 15 48
101954272 1 1 0 0 0
388685 172 53 52 80 46
644591 14 1 2 6 2
25832 1409 1129 1293 878 882
100996763 802 768 749 356 580
440672 462 5091 4563 1282 4166
9659 10182 18069 18125 4173 16115
400818 436 365 398 267 299
653513 1870 2472 2170 429 2069
100996761 20 30 37 32 48
101060226 937 809 929 712 603
653598 55 19 23 50 19
57234 189 117 114 129 77
644634 30 12 12 6 4
100189228 0 0 0 0 0
102723955 9 3 3 9 2
100189059 0 0 0 1 0
440689 173 87 67 279 43
2209 118 40 41 181 27
101927767 16 12 16 45 3
653604 16 3 0 18 1
8370 75 67 79 425 61
105371405 202 127 120 1153 124
126961 42 7 8 244 13
8337 279 115 99 1435 70
337873 34 17 24 198 17
723790 880 259 275 4595 205
333932 11 9 7 102 4
554313 27 14 17 130 24
8349 11996 4632 4183 6669 4493
8338 57 3 4 56 1
DMSO_24rec_Ind3 DOX_144rec_Ind3 FLUO_144rec_Ind3 DMSO_144rec_Ind3
100287102 0 0 0 1
653635 162 243 272 176
102466751 1 5 6 7
100302278 0 0 0 0
645520 0 2 0 0
79501 0 0 0 0
729737 337 315 509 308
102725121 0 0 0 1
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102465909 3 5 0 3
729759 0 0 0 0
100132287 138 236 256 190
105378947 1 4 1 1
101928626 0 0 0 0
102465432 2132 3335 3345 1629
81399 0 1 0 0
100133331 168 218 286 168
100288069 20 13 10 14
400728 3 0 9 8
79854 14 41 29 28
643837 2230 709 2604 2008
284593 0 2 2 0
100130417 18 22 18 16
148398 29 31 30 19
26155 1550 1586 1957 1488
339451 206 389 502 292
84069 8 14 6 4
84808 2 8 3 1
57801 750 673 1160 1050
9636 160 127 137 122
375790 8901 12027 12166 8832
105369174 0 3 0 1
105378948 4 5 5 4
401934 1 0 0 0
54991 278 271 343 287
254099 0 0 0 0
406984 0 0 0 0
406983 0 0 0 0
554210 0 0 0 0
254173 0 0 0 0
8784 1 1 3 1
7293 0 1 0 2
51150 4641 6055 5897 4501
126792 1198 1364 1534 1176
388581 6 33 23 18
118424 880 859 991 768
6339 130 250 298 205
116983 1277 1807 1753 1394
102465434 0 0 1 0
126789 167 156 183 128
54973 1116 1344 1807 1429
102465435 0 1 0 0
80772 541 551 670 553
83756 32 84 32 47
1855 1023 1232 1500 1178
102466740 0 0 0 0
54587 193 178 177 146
54998 1026 962 1410 1019
81669 2378 3582 4608 3211
148413 235 230 279 242
55052 1032 941 1271 984
441869 1 0 0 0
643965 0 1 0 0
102724312 10 4 7 6
64856 232 96 150 119
219293 18 27 35 22
83858 395 817 876 588
55210 688 794 1019 778
339453 65 35 85 81
29101 1694 1655 2064 1480
643988 626 386 572 456
142678 1989 2170 2792 2165
8510 509 280 1112 859
984 948 944 1261 960
728661 2958 2387 3840 2968
8511 985 585 2047 1640
728642 869 1205 1288 1010
9906 1400 1027 2052 1511
65220 732 851 1043 784
2782 7010 5869 7769 6138
105378949 14 15 22 12
163688 2 10 7 6
339456 8 1 2 3
85452 24 4 30 15
2563 0 0 2 1
5590 808 441 855 637
100506504 48 57 70 41
199990 563 532 669 506
105378592 3 13 16 13
6497 1643 1458 2173 1630
105378594 0 3 5 0
79906 87 95 121 89
100129534 23 17 37 34
11079 2687 3075 3215 2482
5192 289 322 331 291
9651 7 9 13 10
55229 1110 862 1675 1206
388585 1 3 5 0
115110 0 0 0 0
8764 0 1 0 0
100996583 1 0 0 0
127281 450 340 496 408
79258 1 3 3 4
100287898 11 26 24 18
140625 0 0 0 0
440556 601 106 738 670
63976 985 376 1621 1403
100422968 0 0 0 2
27237 93 143 128 73
1953 812 942 939 736
693135 0 0 0 0
127262 1011 851 1111 875
49856 245 327 335 237
7161 11 17 23 23
57212 749 618 1040 781
148870 43 33 80 71
388588 66 29 56 32
57470 2041 1566 2581 1928
9731 722 652 828 638
1677 65 99 98 72
339448 209 289 350 247
100133612 0 6 6 1
728716 0 0 0 0
284661 0 0 0 0
55966 0 0 0 2
100616489 0 0 0 0
100616421 0 0 0 0
261734 136 139 184 134
8514 43 59 100 57
26038 4 31 11 12
6146 3061 2052 3225 2725
102724450 28 28 22 27
388591 3867 3214 6515 4444
23463 3469 3344 4365 3597
148645 4 3 8 7
390992 0 0 0 0
387509 240 127 187 155
11332 450 373 383 309
54626 1 11 0 1
83715 10 13 9 5
100422975 0 0 0 0
8718 161 512 227 156
57449 730 1495 800 479
79707 782 723 1016 791
80835 0 4 0 0
3104 254 364 547 318
9903 3944 3881 4518 3307
148479 758 562 954 768
90326 138 150 219 196
55735 1590 1105 1759 1369
100505887 0 0 0 0
23261 828 473 929 709
9341 2218 1556 2042 1625
8863 414 120 637 515
10911 3 3 0 0
3604 118 29 44 49
11315 3185 2720 3673 2947
54206 666 1089 514 373
102724539 0 0 0 0
50651 52 84 66 46
473 3585 4117 5134 3838
102724552 2 1 6 2
2023 35071 12460 32305 22978
102465436 0 0 0 0
100505975 4 8 9 1
765 0 3 0 0
155184 0 0 0 0
6518 1 3 0 0
80045 143 71 111 106
407040 2 5 6 9
9563 1992 1603 2910 2233
80176 99 109 85 61
100506022 6 6 11 11
84275 159 111 196 123
199953 1066 1045 1535 1279
5293 154 165 201 146
644997 0 0 0 0
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22883 5139 5788 7157 5311
56998 921 541 921 743
84328 879 451 905 791
64802 951 614 1147 950
100847055 0 6 2 1
105376719 0 1 0 0
116362 43 45 53 15
10277 2836 1920 3393 2601
23095 6715 4009 8173 6360
100422970 0 0 0 0
5226 2977 3573 3346 2693
100526739 0 1 2 0
378708 51 19 45 23
1325 8 17 15 13
1676 907 653 1070 919
5195 516 494 710 631
54897 4522 3112 6398 4805
148345 14 14 16 12
23435 3928 2954 4662 3557
10747 87 95 129 86
6723 677 867 795 605
5394 1307 1184 2035 1471
2475 3868 2406 5118 3867
100873935 0 0 1 0
10218 5 7 3 11
29914 723 694 984 725
57540 2 7 5 0
101929181 0 0 0 0
26232 244 297 285 240
93611 866 816 1254 849
26270 40 36 75 39
10459 433 277 406 326
374946 4 9 4 0
57085 314 470 428 339
284498 0 0 0 0
102724659 0 0 0 0
4524 2177 1254 3268 2541
1185 1275 1476 1744 1313
100379251 10 66 32 12
4878 92378 173381 111368 83104
4879 29337 110280 22197 15723
26829 0 4 1 0
90231 2020 2338 2554 1857
5351 8285 8783 8744 6251
9927 15593 11272 18482 14053
60672 341 783 455 326
102466982 0 0 0 0
943 0 6 0 0
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7133 4 3 2 4
100616438 0 0 0 0
55187 2540 1934 3633 2497
677885 3 5 13 8
9249 220 300 312 225
102466722 0 0 0 0
343066 0 2 0 0
126767 0 0 0 0
93190 0 0 0 0
390999 0 0 1 0
65121 1 1 5 1
440560 0 0 2 0
343069 6 2 11 3
65122 2 0 7 0
400735 0 0 1 0
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441871 2 2 0 0
440561 0 1 0 0
653606 0 0 0 0
102724742 0 0 1 0
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441873 0 0 0 0
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645354 5 0 3 0
645359 0 0 1 1
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343070 0 0 1 0
400736 1 0 2 0
391003 0 0 0 0
343068 0 0 0 0
391002 0 1 0 0
645382 1 0 1 0
653619 1 0 0 0
729528 1 0 4 0
645414 0 0 0 0
391004 0 0 0 0
645425 0 0 0 0
126755 15 40 15 16
10630 2357 1211 2042 1800
7799 1481 1003 1811 1355
23254 199 201 324 215
200197 21 5 15 4
55092 339 175 254 193
727684 2 2 2 1
114827 3 5 2 1
101927417 1 2 0 0
79180 282 135 200 121
11330 1 1 0 0
63036 0 1 2 1
51032 2 1 14 6
842 796 436 834 581
23341 1598 1607 1868 1431
79814 16 7 18 12
84301 137 64 146 99
6248 122 102 124 104
23207 2622 2407 3530 2656
284723 28 55 76 50
388595 0 0 1 0
54751 2418 2003 2288 1844
440567 1466 994 1586 1334
729614 180 180 294 202
23013 1902 1870 2388 1837
7709 544 624 741 542
101928566 0 0 0 0
149563 0 0 0 0
27129 53159 19730 70102 56156
1187 76 102 93 82
1188 275 56 212 151
348487 50 34 71 74
1969 3488 9932 5419 3787
128272 36 62 35 26
101927546 14 11 9 13
79363 124 109 126 132
54455 1025 661 1356 954
26099 2934 2381 3108 2842
374955 0 2 1 0
55707 242 413 327 274
114819 114 194 234 164
26871 0 0 0 0
100126512 0 0 2 0
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9653 3654 4106 5284 11672 7448
339524 27 8 6 23 16
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5586 855 3530 3958 3084 5927
2959 2254 425 452 946 789
56267 241 295 403 456 645
494115 363 1014 1253 1469 1775
2635 32 163 317 420 529
2633 11 28 47 14 49
2634 60 195 443 739 485
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115362 2 1 0 3 2
729930 0 1 2 3 7
163351 19 6 3 17 5
400759 1 0 0 0 2
23507 798 1105 1817 2125 2375
84230 1179 486 811 982 1120
400761 29 27 26 25 41
55144 614 808 1070 813 1673
105378846 0 0 0 1 2
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284695 304 962 1172 1217 1675
343472 0 0 0 0 0
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164045 17 78 73 25 115
8317 357 186 312 236 283
7049 559 398 414 96 817
676 4 1 0 1 0
253152 11 35 74 33 122
646817 40 48 81 88 103
284697 10 17 32 20 63
23285 46 169 181 146 312
388649 0 4 3 1 4
11146 166 374 455 328 758
79871 339 439 528 604 712
2672 340 6 6 54 3
7813 1320 975 1353 1368 1710
6125 5038 7530 8995 10855 13233
388650 378 663 694 289 1261
6083 0 3 1 3 6
26782 0 3 2 2 7
22823 539 628 770 766 1033
50999 3633 3814 4853 3187 6803
343099 325 127 215 173 207
100131564 262 387 428 1029 635
1810 2262 1123 1282 2071 1921
54874 1804 1110 1348 2041 2018
100126520 2 1 0 1 0
8412 185 70 81 478 100
100129046 19 0 0 4 1
100126348 1 0 0 0 0
100189133 10 1 0 3 1
30836 2171 1355 1630 2173 2340
2730 483 396 462 734 625
24 2 0 1 25 0
9411 1619 1283 1726 1238 2192
5825 960 2879 3132 2498 5296
2152 326 264 335 191 579
101928079 10 15 18 28 27
100616321 0 0 1 0 0
126969 103 65 87 124 137
1266 4607 3404 4602 5346 6564
105378862 195 38 27 27 40
729970 436 121 128 82 195
199857 60 157 163 199 258
101928098 0 0 0 3 0
148534 2338 4934 4491 1937 7755
100527978 39 49 74 46 102
101928118 20 8 8 5 14
25950 129 319 370 274 520
440594 0 0 0 0 0
100996635 0 0 0 0 0
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101928241 0 0 0 1 0
58155 487 844 926 1036 1381
1806 25 18 61 59 37
100873932 0 0 0 0 0
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400765 0 0 0 7 3
100616452 0 1 2 1 0
406928 0 0 0 0 0
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51375 1081 487 667 302 873
163404 337 1 2 18 0
100129620 0 0 0 0 0
9890 16 22 12 14 32
101928270 0 0 0 0 0
54873 2 12 10 8 28
391059 149 546 633 687 987
178 2371 11043 8896 3700 14137
23443 360 761 1045 1120 1512
64645 1211 1468 1926 1726 2996
163786 61 163 236 161 238
54482 148 403 472 545 648
127495 47 1330 958 710 1790
1629 714 1804 1803 2204 2861
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8634 883 1433 1552 1645 2348
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54112 53 5 4 28 3
100128787 0 0 0 0 0
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2135 1449 1079 1277 783 1936
148867 1111 661 909 1163 1209
51611 133 658 745 688 1036
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101928334 0 2 0 0 2
101928370 151 142 180 232 277
1901 518 6228 7059 2260 11481
104355286 0 0 0 0 0
118427 0 12 10 8 6
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55599 568 993 932 877 1407
280 621 750 684 537 972
648740 20 32 33 25 18
279 27 18 9 8 21
276 16 19 9 13 21
277 1 2 5 1 3
278 9 5 5 7 10
100129138 0 1 5 1 4
101928476 0 0 0 1 0
55170 689 790 1000 1091 1424
22854 37 5 5 4 7
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284611 903 659 748 1092 1120
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55119 1332 1513 1801 1953 2298
163479 21 0 2 8 6
6814 1354 1301 1585 2041 2489
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23155 730 1472 1853 1086 2881
22911 2806 885 1085 1246 1541
6884 690 347 392 556 520
56900 601 1372 1504 1422 2280
677766 6 5 7 5 10
127003 15 4 0 4 0
57535 216 13 14 41 18
6301 2745 2378 2836 5299 4215
1952 5845 1383 2113 1191 2382
84722 28 44 86 8 60
343263 13 15 20 43 30
6272 7295 9650 13605 5322 20027
5686 1751 1458 1766 2539 2412
284612 1957 1124 1222 1153 1910
127002 295 204 175 238 279
284613 248 372 502 960 650
57463 273 620 748 526 1106
83873 30 57 45 21 75
2773 820 1039 1598 2782 2090
406974 1 1 3 0 0
2780 18 17 38 22 35
271 1316 972 1331 1315 1751
2948 697 1035 1144 754 1575
2946 323 482 481 259 829
2944 12 15 19 7 24
2949 6 18 11 12 12
2947 2222 815 773 601 1198
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1435 3491 1323 1371 3026 2093
10768 3465 2046 2479 2768 3771
85369 1577 1589 1844 2244 2792
257 11 17 42 48 36
104355139 3 0 1 1 0
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100873974 168 2 2 6 0
3749 1920 632 575 448 796
440600 17 7 4 19 6
64783 1210 342 413 328 495
9122 8 11 30 21 48
10542 485 1332 1273 1620 2004
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84432 2 1 5 1 8
643160 2 16 8 6 7
440602 6 22 10 14 13
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128338 400 1076 1207 984 1914
10390 771 632 749 1089 1116
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149620 1 0 0 0 0
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441897 1 0 0 0 0
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79084 1648 1402 1669 2629 2251
515 3357 6173 5869 5836 9446
128346 83 59 46 45 73
57413 0 0 0 1 1
140 1 0 0 0 0
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5906 1157 1445 1614 2217 2462
55924 3732 1835 2179 1676 3074
100506343 135 45 37 45 54
101928718 86 16 19 7 13
11218 679 747 888 840 1329
3752 76 29 21 6 35
100874295 0 0 0 0 0
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55917 580 891 1262 1504 1628
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54879 345 393 431 616 647
829 2362 3512 4235 6995 6302
4343 1680 1170 1529 1948 2256
389 3633 4507 6088 10756 8783
333926 709 83 99 316 145
284467 154 0 0 2 0
100996702 6 10 12 15 13
6566 9908 23777 24297 10235 42639
246182 10 18 14 13 14
100506392 17 52 34 50 66
100996251 22 15 7 24 22
9860 158 642 589 774 996
643441 0 0 0 1 0
260425 1197 496 670 340 957
10745 727 1170 1591 1644 2165
54665 590 490 575 557 889
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26191 31 2 5 4 3
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270 6 0 0 1 0
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7812 17840 16515 18048 24160 26742
80143 1041 1687 1955 2303 2624
6847 1 0 0 7 0
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4803 74 28 33 193 99
81839 857 700 1016 522 1353
845 8539 7850 6945 2551 14038
4808 535 4 2 79 5
101928995 3 1 1 1 0
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55356 241 565 574 329 903
126868 18 15 25 10 33
476 30468 55183 65536 44977 106428
84852 20 44 52 102 63
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965 199 445 574 415 1042
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3321 8376 6804 8768 3812 11904
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9398 55 24 41 20 57
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10905 2362 3127 4100 3059 6009
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54834 1243 401 528 732 666
10885 1683 851 1201 1450 1706
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90874 779 754 817 769 1286
26227 723 581 894 289 1104
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9554 669 1935 2305 2829 3379
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728841 483 619 806 829 1131
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100996724 1379 4333 3897 1451 6215
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337875 6 1 3 7 2
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653464 949 480 679 1097 1032
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791227 0 0 0 0 0
105369140 1 2 2 0 5
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100996713 6 4 6 12 7
554282 303 28 33 20 35
100996712 671 256 374 454 463
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647135 741 368 492 696 670
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645142 1 0 0 0 0
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101929780 215 110 175 183 200
653519 271 306 386 361 614
5174 23 9 10 21 20
11126 22 26 39 37 54
27246 614 1668 1959 1617 3042
10623 840 637 698 1025 1099
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10401 907 876 1038 1384 1584
102466191 0 1 0 0 0
148741 6 3 0 3 1
8515 97 156 172 54 310
8799 247 601 638 745 1001
114814 4 35 30 23 49
9939 1933 1657 1894 4391 2588
128077 233 736 1004 1214 1428
84265 852 538 568 639 915
284615 200 178 210 129 304
10628 766 4772 6892 5264 14227
148738 10 50 35 7 63
100189384 0 0 0 0 0
100189064 1 0 0 0 0
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100189198 0 0 0 0 0
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100132406 308 317 403 278 434
388677 272 362 431 315 543
170688 609 3984 4196 2772 6754
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105373468 7 15 18 7 25
149013 681 657 839 838 1142
728989 21 49 50 19 81
100189334 0 0 0 0 0
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5565 3752 2327 2229 2971 3683
171423 7111 7944 9772 9298 15186
2330 158 122 146 224 222
9557 496 536 672 324 865
100289211 0 15 10 3 18
607 700 1078 1263 1199 1933
51205 193 407 406 237 629
2702 9 630 977 39 1642
2703 6 0 0 3 0
51463 300 400 454 388 793
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100189140 0 0 0 0 0
26864 0 0 0 0 0
100189193 0 0 0 0 0
200030 267 281 349 323 432
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101954272 0 6 0 1 0
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644591 4 3 6 4 3
25832 619 721 814 501 961
100996763 366 436 507 373 638
440672 805 5043 5296 3418 8806
9659 5689 16873 16137 5066 24700
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653513 467 1599 1440 476 2464
100996761 37 26 31 28 47
101060226 549 572 692 488 847
653598 14 13 30 64 46
57234 183 96 117 143 168
644634 20 3 15 4 15
100189228 0 0 0 0 0
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2209 202 40 37 265 40
101927767 39 12 8 68 17
653604 11 0 2 16 7
8370 186 56 64 506 100
105371405 529 136 151 1683 200
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337873 96 23 23 283 31
723790 2121 277 251 3299 335
333932 55 5 4 69 4
554313 37 13 19 127 27
8349 8874 4357 3968 13125 6510
8338 63 1 3 37 7
DMSO_24rec_Ind4 DOX_144rec_Ind4 FLUO_144rec_Ind4 DMSO_144rec_Ind4
100287102 1 6 0 2
653635 311 368 351 297
102466751 12 17 16 4
100302278 1 0 0 0
645520 0 0 0 0
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400728 2 3 2 3
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643837 1899 1329 2413 2144
284593 2 1 2 0
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148398 10 8 19 17
26155 1699 1624 1491 1441
339451 350 349 298 292
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84808 7 5 2 1
57801 1161 964 909 1008
9636 144 123 111 137
375790 14759 16610 11017 14190
105369174 6 6 9 14
105378948 14 9 13 23
401934 2 0 0 0
54991 240 216 248 273
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118424 940 854 816 769
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116983 1663 1951 1621 1490
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83756 30 57 37 40
1855 1216 1169 1225 1154
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54998 1060 958 1004 879
81669 4085 4225 3772 3973
148413 261 236 258 217
55052 1033 842 948 885
441869 0 0 1 0
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102724312 17 12 8 21
64856 670 234 375 656
219293 51 73 40 42
83858 748 812 647 621
55210 744 751 655 708
339453 67 47 63 56
29101 1608 1220 1305 1254
643988 146 128 156 190
142678 2108 2438 2088 2000
8510 548 519 608 473
984 1041 1020 987 983
728661 2405 2200 2197 2225
8511 1059 970 1189 910
728642 811 834 720 692
9906 1477 1362 1470 1391
65220 1120 988 889 925
2782 8204 6062 6490 7735
105378949 16 11 5 10
163688 4 7 4 3
339456 2 2 6 2
85452 1 2 6 6
2563 1 0 2 2
5590 772 613 749 580
100506504 35 50 37 43
199990 699 476 515 563
105378592 9 11 7 8
6497 1690 1681 1555 1747
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100129534 22 15 13 19
11079 3215 3318 2710 2801
5192 328 263 258 233
9651 8 15 6 17
55229 1155 1297 1244 1163
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100996583 0 0 0 0
127281 585 357 559 529
79258 1 2 3 2
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140625 0 0 0 0
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63976 1072 743 1295 1276
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27237 100 43 88 83
1953 1606 1021 1082 1541
693135 0 0 0 0
127262 1316 1078 1146 1277
49856 289 262 254 243
7161 10 7 4 7
57212 971 831 907 909
148870 34 28 32 30
388588 27 26 28 31
57470 1898 1708 1903 1768
9731 1028 962 941 998
1677 71 78 63 79
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261734 198 157 195 189
8514 58 45 89 68
26038 16 33 18 9
6146 2462 1527 2179 2240
102724450 39 46 27 21
388591 5343 4866 5346 4798
23463 3712 3645 3270 3436
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387509 261 225 230 243
11332 637 444 458 608
54626 2 8 0 5
83715 7 14 15 9
100422975 0 0 0 0
8718 243 320 190 178
57449 1022 1415 804 758
79707 750 691 622 676
80835 1 2 1 4
3104 387 387 362 318
9903 3475 3834 2812 2959
148479 937 690 900 832
90326 189 159 173 178
55735 1465 1223 1398 1348
100505887 0 0 0 0
23261 783 515 709 629
9341 2650 1750 2101 2368
8863 264 157 484 471
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3604 293 109 107 247
11315 3308 2589 2892 2916
54206 1262 1153 746 1036
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473 3784 4003 3557 3783
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22883 6784 7193 6210 6700
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10277 2518 2059 2383 2399
23095 6952 7317 6578 6948
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1325 4 8 6 6
1676 846 716 768 817
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54897 4253 4639 4001 4010
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23435 3801 3316 3393 3510
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6723 878 538 647 743
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2475 4143 3830 3929 4176
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29914 771 626 690 712
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4524 2435 2175 2634 2494
1185 1551 1864 1545 1589
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4878 66249 100302 49985 53451
4879 13575 33896 8860 12978
26829 2 1 0 1
90231 2302 2549 2264 2347
5351 10062 10591 9522 10518
9927 12089 14011 11552 12192
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126755 6 8 6 4
10630 1637 849 1558 1450
7799 1501 1386 1415 1381
23254 221 168 198 171
200197 15 2 7 17
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11330 0 1 0 0
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842 605 491 618 607
23341 1652 1770 1631 1622
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6248 127 92 121 118
23207 3377 3146 3006 3031
284723 41 50 47 45
388595 1 0 0 0
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23013 2010 2281 2046 2338
7709 670 596 563 528
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27129 44410 34177 48873 44131
1187 111 94 108 85
1188 223 123 172 165
348487 47 35 60 51
1969 4887 8251 3886 4691
128272 48 35 44 35
101927546 14 16 12 8
79363 143 123 123 114
54455 954 834 998 1106
26099 3159 2265 2635 2795
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26871 3 0 0 0
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7195 0 0 0 0
55672 549 591 522 576
84809 269 308 276 270
11209 97 79 90 71
26869 2 0 1 0
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284729 2 0 0 3
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11223 49 40 60 51
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7193 3 0 4 3
791226 0 0 0 0
9696 359 397 327 388
26870 3 2 0 3
4237 393 161 469 534
23400 1688 1401 1396 1670
6390 2035 1698 1967 2018
11240 41 19 37 47
29943 10 6 8 6
51702 0 2 0 0
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DOX_24_Ind5 FLUO_24_Ind5 DMSO_24_Ind5 DOX_24rec_Ind5 FLUO_24rec_Ind5
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676 3 0 0 6 0
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284697 5 24 28 29 39
23285 41 145 135 175 207
388649 1 2 2 2 4
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79871 301 498 490 532 527
2672 601 3 14 29 9
7813 1138 1452 1289 1315 1789
6125 5928 7790 7586 7651 7298
388650 551 908 854 434 897
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343099 252 173 220 211 143
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1810 2323 1284 1192 2028 1381
54874 1468 1835 1540 2353 2105
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30836 1705 1690 1564 2340 1775
2730 521 349 312 638 436
24 21 1 6 17 7
9411 1753 1483 1278 1622 1445
5825 1151 2635 2388 2221 2805
2152 913 422 387 869 548
101928079 6 5 9 14 2
100616321 0 0 0 0 0
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1266 5201 4380 4131 6165 5094
105378862 119 22 16 33 16
729970 259 109 82 88 81
199857 78 154 149 162 112
101928098 3 1 1 1 1
148534 2356 2864 2884 1137 2592
100527978 22 48 63 55 45
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25950 107 313 262 225 302
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58155 425 724 693 880 775
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51375 973 617 554 396 578
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391059 339 729 624 468 637
178 3952 8153 7998 3143 7346
23443 689 1316 1186 1256 1336
64645 1508 2204 1870 1829 2066
163786 100 184 195 226 167
54482 116 504 412 550 508
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1629 907 1515 1318 1624 1599
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8634 1145 1610 1373 1529 1620
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2135 1811 1155 1150 679 1203
148867 1751 1463 1312 1518 1557
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1901 823 7808 6359 2638 7629
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280 573 499 445 446 479
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279 32 4 8 12 9
276 30 14 11 8 16
277 4 1 0 3 2
278 19 4 1 5 11
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29957 1067 1918 1862 1844 2117
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55119 1057 1700 1557 2003 2036
163479 59 6 10 7 18
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22911 2340 1315 1114 1338 1461
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343263 61 74 68 99 78
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284612 1111 1011 860 639 868
127002 197 188 158 325 183
284613 181 426 393 740 457
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2773 771 1860 1750 2534 1760
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271 1178 1556 1373 1805 1685
2948 669 834 742 735 778
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2947 1634 723 633 580 686
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1435 2877 1338 962 2327 1165
10768 3150 2530 2306 2686 2626
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3749 972 802 622 538 627
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515 4346 5398 4875 5143 4760
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140 0 0 0 0 0
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55924 2167 2483 1973 1547 2062
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101928718 64 17 24 10 18
11218 646 1001 895 907 1076
3752 111 49 42 33 49
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55917 846 1741 1350 2499 1798
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7482 75 27 26 32 33
54879 367 563 506 680 624
829 2604 4421 3999 6329 4281
4343 1739 1838 1566 2067 1993
389 4005 5342 4492 7278 5255
333926 495 80 64 171 56
284467 86 1 2 4 3
100996702 8 8 7 10 9
6566 9186 16978 15849 10053 16357
246182 8 11 13 10 17
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54665 703 484 492 597 549
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270 7 1 1 7 0
4893 1309 2728 2468 3142 2364
7812 18658 16733 15295 20448 17019
80143 1220 1895 1625 2192 1824
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845 31012 24799 22068 9874 25461
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476 35149 52358 44832 34799 52192
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27246 436 1765 1566 1418 1696
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8799 196 648 511 613 578
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9939 1774 1611 1525 2881 1548
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84265 721 437 460 551 419
284615 156 248 217 157 214
10628 413 4224 3067 4035 5451
148738 17 41 49 5 32
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105373468 11 24 24 17 42
149013 1127 1075 907 1006 1380
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5565 4501 3251 2731 5186 2824
171423 15147 12817 11637 8023 14748
2330 157 141 141 173 179
9557 626 869 764 457 834
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607 808 1088 822 1124 1096
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2702 28 1303 1145 65 1339
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26864 0 1 0 0 0
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25832 1044 916 823 625 977
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9659 4947 10673 9611 5798 10567
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653598 32 24 24 37 27
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723790 1399 182 144 1571 162
333932 41 3 3 21 2
554313 56 17 11 40 12
8349 5498 2909 2635 6253 2672
8338 69 4 4 26 2
DMSO_24rec_Ind5 DOX_144rec_Ind5 FLUO_144rec_Ind5 DMSO_144rec_Ind5
100287102 9 7 6 6
653635 177 182 158 180
102466751 7 5 5 4
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57801 1498 1041 1125 1119
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375790 29485 24605 22049 22556
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83858 853 721 657 629
55210 906 853 827 789
339453 49 46 35 58
29101 1374 1375 1097 1235
643988 222 362 264 264
142678 1942 2212 1759 1746
8510 443 562 377 433
984 1049 1158 946 912
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2782 9332 8715 8407 8705
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11079 3476 3781 3303 3287
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63976 515 760 791 725
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57470 1695 1843 1505 1584
9731 989 1023 893 1024
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6146 2191 1793 1623 1775
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388591 6003 6357 6488 5784
23463 3646 4080 3452 3475
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11332 1194 874 792 883
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57449 2595 2087 1785 1556
79707 768 850 649 719
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3104 469 438 367 386
9903 4682 4913 4170 4352
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11315 2489 2333 2291 2101
54206 3777 2096 1944 1917
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26099 3065 3022 2609 2938
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23400 2829 2271 2235 2274
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24 3 4 1 12 1
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178 1852 15875 11076 4645 13311
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554313 115 29 21 101 14
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DMSO_24rec_Ind6 DOX_144rec_Ind6 FLUO_144rec_Ind6 DMSO_144rec_Ind6
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7812 19218 19161 18784 23838
80143 1334 2026 1929 2454
6847 0 0 0 3
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10100 427 966 941 974
4803 147 72 57 313
81839 1034 988 1073 649
845 11402 9741 9477 4278
4808 565 4 2 91
101928995 3 0 1 3
101928977 2 1 2 1
55356 321 698 701 481
126868 22 19 39 9
476 32342 32713 33808 26879
84852 19 47 35 75
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3321 9651 5929 6541 2667
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5738 14049 13911 14763 4280
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54834 1359 479 468 623
10885 2031 1217 1285 1369
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3283 3 0 0 3
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644242 42 19 28 73
90874 1313 1220 1154 1690
26227 919 973 947 530
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4853 13783 9865 10818 8076
9554 883 2554 2466 2716
105378954 60 9 5 30
728841 542 797 701 897
767846 14 28 28 23
100996724 1081 2103 1960 1328
101929796 151 126 118 139
101060684 936 793 952 865
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101954277 2 2 0 0
653505 22 8 21 26
728855 154 95 81 85
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100189257 0 0 0 0
105369199 1 1 1 1
100996721 14 10 15 7
100126488 0 0 0 0
101929798 0 0 0 0
2210 31 1 2 24
100996318 10 1 3 3
337875 16 3 4 10
653820 388 112 101 36
653464 1062 1385 1391 1393
100873165 5 8 2 7
647121 14 24 35 17
101929815 4 1 1 1
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645166 8 55 33 56
647135 908 1145 1113 1069
728833 368 52 59 38
101929805 2 0 0 0
102724235 20 11 15 7
103091866 105 41 57 24
645142 1 0 0 1
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7214 0 0 0 0
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100126510 0 0 0 0
101060254 17 23 19 21
101929780 157 143 175 185
653519 354 399 333 328
5174 37 9 20 20
11126 32 27 54 52
27246 561 2077 1975 2335
10623 819 851 830 1032
200035 194 113 90 244
10401 909 1210 1234 1393
102466191 0 0 1 1
148741 15 2 2 1
8515 152 199 208 71
8799 281 732 635 661
114814 13 23 13 14
9939 1826 2040 1811 3717
128077 351 1487 1477 1470
84265 931 600 553 639
284615 165 382 348 178
10628 330 3499 3134 3160
148738 11 35 28 15
100189384 0 0 0 0
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170688 702 2661 2601 1707
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105373468 14 22 22 19
149013 912 751 842 916
728989 13 31 15 15
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5565 3612 2828 2358 3832
171423 13853 12331 13789 7243
2330 144 189 148 219
9557 677 826 853 406
100289211 6 7 9 2
607 903 958 1020 947
51205 247 300 361 302
2702 90 3908 3992 421
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51463 367 420 450 413
100034743 33 11 18 19
100189140 1 0 0 0
26864 2 0 0 2
100189193 0 0 0 0
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101954272 4 3 4 1
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644591 7 5 2 5
25832 1042 758 838 663
100996763 997 767 799 706
440672 944 3605 3322 2239
9659 4613 8619 8608 4842
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653513 385 875 765 500
100996761 28 25 17 28
101060226 714 584 656 553
653598 25 27 29 36
57234 244 130 89 109
644634 22 13 6 5
100189228 0 0 0 0
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653604 19 2 2 1
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337873 157 19 27 71
723790 1682 237 179 1724
333932 70 1 5 22
554313 73 9 9 45
8349 5606 2249 2142 6614
8338 73 8 5 18
FLUO_24rec_Ind6REP DMSO_24rec_Ind6REP DOX_144rec_Ind6REP
100287102 2 3 1
653635 196 235 198
102466751 4 9 10
100302278 0 0 0
645520 0 0 0
79501 0 0 0
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105378947 8 4 7
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102465432 1159 1244 1308
81399 1 1 0
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100288069 20 17 17
400728 1 2 8
79854 12 16 30
643837 1087 964 921
284593 0 0 1
100130417 12 10 16
148398 5 9 8
26155 1696 1862 1786
339451 323 415 369
84069 23 25 16
84808 4 5 5
57801 974 1425 944
9636 235 355 181
375790 20081 23995 18295
105369174 1 8 7
105378948 7 16 9
401934 0 2 2
54991 257 321 225
254099 0 0 0
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554210 0 0 0
254173 0 0 0
8784 2 2 4
7293 3 1 1
51150 5191 6308 6308
126792 1269 1495 1230
388581 25 33 30
118424 913 1073 973
6339 140 140 142
116983 1523 1902 1860
102465434 0 0 0
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54973 1156 1265 1099
102465435 0 0 1
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1855 972 1222 1252
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81669 4943 5171 4100
148413 272 284 224
55052 785 942 727
441869 0 0 0
643965 1 0 0
102724312 6 6 3
64856 197 305 98
219293 18 19 15
83858 499 582 588
55210 628 753 665
339453 54 83 40
29101 1552 1658 1243
643988 170 228 223
142678 1341 1420 1663
8510 318 319 389
984 961 975 1011
728661 3611 3790 2979
8511 622 650 658
728642 854 941 1080
9906 947 898 878
65220 1185 1360 962
2782 11134 13058 8117
105378949 15 11 16
163688 9 10 12
339456 3 8 5
85452 2 0 4
2563 0 0 0
5590 651 680 524
100506504 45 48 51
199990 621 823 580
105378592 13 13 16
6497 2068 2395 1879
105378594 1 3 6
79906 115 94 98
100129534 30 32 30
11079 2806 3399 2888
5192 273 342 275
9651 19 16 12
55229 689 720 803
388585 0 0 2
115110 0 0 0
8764 0 2 1
100996583 1 1 0
127281 459 544 403
79258 2 2 0
100287898 7 15 15
140625 0 0 0
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63976 634 504 487
100422968 0 0 0
27237 72 90 89
1953 2285 2848 2027
693135 0 0 0
127262 1357 1499 1208
49856 221 249 224
7161 5 4 13
57212 865 864 708
148870 36 28 20
388588 15 20 22
57470 1535 1646 1503
9731 1028 1007 905
1677 72 79 79
339448 338 347 246
100133612 0 10 8
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55966 1 2 6
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261734 166 212 150
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26038 18 16 65
6146 2450 2714 1696
102724450 6 12 18
388591 3546 3321 4037
23463 3243 3747 2757
148645 1 1 1
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54626 11 5 18
83715 6 1 5
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57449 1172 1190 1298
79707 703 730 624
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3104 320 357 334
9903 3871 4194 3791
148479 983 1093 591
90326 168 205 162
55735 1431 1283 1232
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23261 528 622 456
9341 2977 3124 1800
8863 198 170 170
10911 7 10 0
3604 234 339 82
11315 2638 2924 2048
54206 3079 3548 1453
102724539 1 1 0
50651 71 90 95
473 5118 5116 4443
102724552 1 3 0
2023 13253 16148 10470
102465436 0 0 0
100505975 3 5 9
765 0 0 1
155184 0 0 0
6518 11 7 12
80045 45 61 59
407040 3 2 3
9563 2133 2331 1875
80176 281 286 202
100506022 16 24 12
84275 124 151 105
199953 593 763 781
5293 293 281 201
644997 0 0 0
101929074 7 10 4
22883 5891 7042 6205
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116362 1 8 1
10277 2573 2570 2099
23095 7174 6794 5742
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5226 3116 3526 3033
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1325 4 11 2
1676 759 1001 692
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23435 3745 3672 3100
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6723 888 1177 766
5394 1669 1774 1432
2475 3698 3679 3369
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29914 770 776 588
57540 9 7 7
101929181 0 0 0
26232 347 405 432
93611 712 762 718
26270 105 110 77
10459 308 371 275
374946 12 10 26
57085 392 482 376
284498 0 0 0
102724659 0 0 0
4524 2104 2065 1903
1185 1076 1268 1352
100379251 32 29 37
4878 119277 120836 198095
4879 24621 27545 52110
26829 0 0 0
90231 1709 2080 1998
5351 9920 13328 9380
9927 9380 9193 10570
60672 397 470 414
102466982 0 0 0
943 2 2 1
102465836 0 0 0
7133 2 7 4
100616438 0 0 0
55187 2460 2238 2248
677885 3 5 1
9249 348 328 319
102466722 0 0 0
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23254 231 202 208
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55092 660 838 374
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23341 1474 1588 1446
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6248 144 142 99
23207 2504 2864 2461
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54751 5413 6398 3413
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23013 2489 2462 2065
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27129 28827 27445 25815
1187 47 47 48
1188 124 114 99
348487 26 28 28
1969 7892 8587 9956
128272 57 72 46
101927546 4 4 6
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54455 817 796 735
26099 3494 3856 2851
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26871 0 0 0
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102724970 7 5 8
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11209 74 71 39
26869 1 1 0
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11223 23 25 27
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26870 1 1 1
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23400 2060 2798 1838
6390 1244 1312 1200
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29943 2 5 8
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126917 824 946 612
23352 9502 9192 8134
23065 3249 3811 3270
51154 613 701 559
246181 5 10 5
22977 7 5 5
100506730 116 110 58
8574 496 607 442
54896 539 640 589
832 4193 4700 3179
100532736 0 0 0
440574 1080 1261 1006
644068 48 50 33
4681 17 24 21
3362 0 0 3
255104 112 129 85
54546 0 0 1
23252 507 521 471
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5322 3 2 10
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127733 24 26 11
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163933 12 6 16
978 3 1 0
65018 813 889 865
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1650 9132 10484 9891
57576 25 30 24
400745 50 57 70
50809 3252 3678 2662
8672 5798 5618 4734
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1889 12261 15297 10581
100506801 5 9 5
84224 144 139 123
249 19 23 23
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84196 3053 3095 2388
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3339 35955 36430 40880
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2046 1 3 0
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23028 2654 2916 1914
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3352 70 61 60
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10236 3502 3864 3298
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6924 1900 1821 1363
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2582 471 581 554
3155 477 564 341
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10772 2497 2576 1932
127294 5 4 12
58985 7 15 15
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57185 2380 2465 2136
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400746 0 2 1
10250 2234 2263 2070
25932 21525 22330 11993
864 7 9 7
102465437 0 1 0
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25949 858 832 743
57035 2771 2830 2152
6007 19 25 17
23585 1977 2056 1649
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26119 496 576 534
57134 2450 2559 2929
57190 5380 6708 4620
56181 2070 2045 2001
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3151 1874 2387 1258
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8289 4660 4466 3985
55650 245 244 226
84243 526 676 490
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388610 716 756 647
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23038 1234 1422 1097
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5724 2 6 5
22826 1550 1605 1283
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6421 5259 5816 4967
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79932 3911 4392 3740
23154 1595 1784 1700
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5690 2047 2278 1606
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26523 882 871 648
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54936 482 533 463
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79647 4076 3987 3260
4725 2149 2268 1927
23499 30413 26016 26414
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5538 1935 2233 2080
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127391 0 0 0
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64789 161 193 154
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9783 782 846 937
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4802 1427 1451 1442
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163732 368 417 558
1503 1522 1955 1217
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2981 0 0 2
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84217 8 5 2
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148534 2088 2051 1985
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178 5579 4845 5068
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170688 2915 2802 2000
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8349 2149 2222 2338
8338 4 4 4
FLUO_144rec_Ind6REP DMSO_144rec_Ind6REP
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54206 2017 2070
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[ reached getOption("max.print") -- omitted 26808 rows ]
fC_Matrix_Full_cpm <- cpm(fC_Matrix_Full, log = TRUE)
boxplot(fC_Matrix_Full_cpm, xlab = "Conditions", ylab = expression("log"[2]* "counts per million"), ylim= c(-10, 20))
Version | Author | Date |
---|---|---|
a07d5bc | emmapfort | 2025-03-19 |
hist(fC_Matrix_Full_cpm, xlab = expression("log" [2]* "counts per million"))
Version | Author | Date |
---|---|---|
a07d5bc | emmapfort | 2025-03-19 |
#first check the number of variables in the cpm file
dim(fC_Matrix_Full_cpm)
[1] 28395 63
#[1] 28395 63
#now use rowMeans to filter out values with mean < 0 (this means rowMeans > 0 is what I'm left with) for each row/gene in the original file
rowMeans_All <- rowMeans(fC_Matrix_Full_cpm)
fC_Matrix_Full_cpm_filter <- fC_Matrix_Full_cpm[rowMeans_All > 0,]
dim(fC_Matrix_Full_cpm_filter)
[1] 14225 63
#[1] 14225 63
#with this I've filtered out a significant number of rows, want ~14000
hist(fC_Matrix_Full_cpm_filter,
main = "Recovery All Individuals log2cpm Filtered",
xlab = expression("log"[2]*"counts per million"))
Version | Author | Date |
---|---|---|
a07d5bc | emmapfort | 2025-03-19 |
boxplot(fC_Matrix_Full_cpm_filter,
main = "Recovery All Individuals log2cpm Filtered",
xlab = "Conditions",
ylab = expression("log"[2]*"counts per million"),
ylim = c(-10,15))
Version | Author | Date |
---|---|---|
a07d5bc | emmapfort | 2025-03-19 |
#save this to an R object
#saveRDS(fC_Matrix_Full_cpm_filter, "data/fC_Matrix_Full_cpm_filter.RDS")
#this will be used to filter my datasets against for filtered counts
#through this, I can save the rownames to have my final filtered dataset for DE
filt_gene_list <- row.names(fC_Matrix_Full_cpm_filter)
#saveRDS(filt_gene_list, "data/filt_gene_list.RDS")
#this is what I'll be using for differential expression analysis (see DE page)
####histogram of total counts, unfiltered####
hist(fC_Matrix_Full_cpm, main = "Recovery All Individuals log2cpm Unfiltered",
xlab = expression("log" [2]* "counts per million"))
Version | Author | Date |
---|---|---|
a07d5bc | emmapfort | 2025-03-19 |
####histogram of total counts, filtered####
hist(fC_Matrix_Full_cpm_filter,
main = "Recovery All Individuals log2cpm Filtered rowMeans > 0",
xlab = expression("log"[2]*"counts per million"))
Version | Author | Date |
---|---|---|
a07d5bc | emmapfort | 2025-03-19 |
####boxplot log2cpm all samples####
boxplot(fC_Matrix_Full_cpm, xlab = "Conditions", ylab = expression("log"[2]* "counts per million"), ylim= c(-10, 20), main = "Boxplots of log2cpm per sample")
Version | Author | Date |
---|---|---|
a07d5bc | emmapfort | 2025-03-19 |
####boxplot log2cpm filtered all samples####
boxplot(fC_Matrix_Full_cpm_filter, xlab = "Conditions", ylab = expression("log"[2]* "counts per million"), ylim= c(-10, 20), main = "Boxplots of log2cpm Filtered rowMeans > 0 per sample")
Version | Author | Date |
---|---|---|
a07d5bc | emmapfort | 2025-03-19 |
Now that I’ve filtered the samples after log2cpm conversion, I can start to make heatmaps and PCA plots to look at my data. I want these plots to have treatment and time as the primary variables.
PCA_data <- fC_Matrix_Full_cpm_filter %>%
prcomp(.) %>%
t()
PCA_data_test <- (prcomp(t(fC_Matrix_Full_cpm_filter), scale. = TRUE))
#ind_num <- c("1", "1", "1", "1", "1", "1", "1", "1", "1","2", "2", "2", "2", "2", "2", "2", "2", "2", "3", "3", "3", "3", "3", "3", "3", "3", "3","4", "4", "4", "4", "4", "4", "4", "4", "4","5", "5", "5", "5", "5", "5", "5", "5", "5","6", "6", "6", "6", "6", "6", "6", "6", "6","6R", "6R", "6R", "6R", "6R", "6R", "6R", "6R", "6R")
#saveRDS(ind_num, "data/ind_num.RDS")
ind_num <- readRDS("C:/Users/emmap/RDirectory/Recovery_RNAseq/Recovery_5FU/data/ind_num.RDS")
####now make an annotation for my PCA####
annot <- data.frame("samples" = colnames(fC_Matrix_Full_cpm_filter)) %>% separate_wider_delim(., cols = samples, names = c("Tx", "Time", "Ind"), delim = "_", cols_remove = FALSE) %>% unite(., col = "Tx_Time", Tx, Time, sep = "_", remove = FALSE) %>% cbind(., ind_num)
#saveRDS(annot, "C:/Users/emmap/RDirectory/Recovery_RNAseq/Recovery_5FU/data/annot.RDS")
#combine the prcomp matrix and annotation
annot_PCA_matrix <- PCA_data_test$x %>% cbind(., annot)
#now I can make a graph where I have filled values for individual! I have seven colors for seven individuals
# I have three fill values for three timepoints
#using annotation matrix above as well as annotated PCA matrix (annot_PCA_matrix)
#extra info for colors in the graph (fill parameter)
fill_col_ind <- c("#66C2A5", "#FC8D62", "#1F78B4", "#E78AC3", "#A6D854", "#FFD92A", "#8B3E9B")
fill_col_ind_dark <- c("#003F5C", "#45AE91", "#58508D", "#BC4099", "#8B3E9B", "#FF6361", "#FF2362")
fill_col_tx <- c("#63666D", "#499FBD", "#DCACED")
#I want the color parameter to be treatment, the shape as time, and individual as number
####PC1/PC2####
annot_PCA_matrix %>% ggplot(., aes(x=PC1, y=PC2, size=10)) +
geom_point(aes(color = Tx, shape = Time)) +
scale_color_manual(values = c(fill_col_tx))+
scale_shape_manual(values = c(15, 19, 17))+
geom_text_repel(aes(label = ind_num))+
theme_bw(base_size = 10)+
ggtitle(expression("PCA of filtered log"[2]*"cpm"))+
guides(size="none")
Warning: ggrepel: 10 unlabeled data points (too many overlaps). Consider
increasing max.overlaps
Version | Author | Date |
---|---|---|
a07d5bc | emmapfort | 2025-03-19 |
####PC2/PC3####
annot_PCA_matrix %>% ggplot(., aes(x=PC2, y=PC3, size=10)) +
geom_point(aes(color = Tx, shape = Time)) +
scale_color_manual(values = c(fill_col_tx))+
scale_shape_manual(values = c(15, 19, 17))+
geom_text_repel(aes(label = ind_num))+
theme_bw(base_size = 10)+
ggtitle(expression("PCA of filtered log"[2]*"cpm"))+
guides(size="none")
Warning: ggrepel: 7 unlabeled data points (too many overlaps). Consider
increasing max.overlaps
Version | Author | Date |
---|---|---|
a07d5bc | emmapfort | 2025-03-19 |
####PC3/PC4####
annot_PCA_matrix %>% ggplot(., aes(x=PC3, y=PC4, size=10)) +
geom_point(aes(color = Tx, shape = Time)) +
scale_color_manual(values = c(fill_col_tx))+
scale_shape_manual(values = c(15, 19, 17))+
geom_text_repel(aes(label = ind_num))+
theme_bw(base_size = 10)+
ggtitle(expression("PCA of filtered log"[2]*"cpm"))+
guides(size="none")
Warning: ggrepel: 16 unlabeled data points (too many overlaps). Consider
increasing max.overlaps
Version | Author | Date |
---|---|---|
a07d5bc | emmapfort | 2025-03-19 |
Now, let’s make some correlation heatmaps These are pairwise across all genes by condition
#correlation heatmap for all samples
####PEARSON FILTERED####
fC_Matrix_Full_cpm_filter_pearsoncor <-
cor(
fC_Matrix_Full_cpm_filter,
y = NULL,
use = "everything",
method = "pearson"
)
####SPEARMAN FILTERED####
fC_Matrix_Full_cpm_filter_spearmancor <-
cor(
fC_Matrix_Full_cpm_filter,
y = NULL,
use = "everything",
method = "spearman"
)
#Now let's graph these correlations
pheatmap(fC_Matrix_Full_cpm_filter_pearsoncor, border_color = "black", legend = TRUE, angle_col = 90, display_numbers = FALSE, number_color = "black", fontsize = 12, fontsize_number = 9)
Version | Author | Date |
---|---|---|
a07d5bc | emmapfort | 2025-03-19 |
pheatmap(fC_Matrix_Full_cpm_filter_spearmancor, border_color = "black", legend = TRUE, angle_col = 90, display_numbers = FALSE, number_color = "black", fontsize = 12, fontsize_number = 9)
Version | Author | Date |
---|---|---|
a07d5bc | emmapfort | 2025-03-19 |
#now annotate these heatmaps
#Factor 1 - Individual
Individual <- as.factor(c(rep("Ind1", 9), rep("Ind2", 9), rep("Ind3", 9), rep("Ind4", 9), rep("Ind5", 9), rep("Ind6", 9), rep("Ind6REP", 9)))
#Factor 2 - Treatment
tx_factor <- c("DOX", "FLUO", "DMSO")
Tx <- as.factor(c(rep(tx_factor, 21)))
#view(Treatment)
#Factor 3 - Timepoint
time_factor <- c(rep("24", 3), rep("24rec", 3), rep("144rec", 3))
Time <- as.factor(c(rep(time_factor, 7)))
####annotation for colors####
annot_col_hm = list(Tx = c(DOX = "red", FLUO = "blue", DMSO = "black"),
Ind = c(Ind1 = "#66C2A5", Ind2 = "#FC8D62", Ind3 = "#1F78B4", Ind4 = "#E78AC3", Ind5 = "#A6D854", Ind6 = "#FFD92A", Ind6REP = "#8B3E9B"),
Time = c("24" = "#046A38", "24rec" = "#0050B5", "144rec" = "#B725AD"))
####annotation for values####
annot_list_hm <- data.frame(Individual = as.factor(c(rep("Ind1", 9), rep("Ind2", 9), rep("Ind3", 9), rep("Ind4", 9), rep("Ind5", 9), rep("Ind6", 9), rep("Ind6REP", 9))),
Tx = as.factor(c(rep(tx_factor, 21))),
Time = as.factor(c(rep(time_factor, 7))))
##add in the annotations from above into the dataframe
row.names(annot_list_hm) <- colnames(fC_Matrix_Full_cpm_filter_pearsoncor)
row.names(annot_list_hm) <- colnames(fC_Matrix_Full_cpm_filter_spearmancor)
####ANNOTATED HEATMAPS####
pheatmap(fC_Matrix_Full_cpm_filter_pearsoncor, border_color = "black", legend = TRUE, angle_col = 90, display_numbers = FALSE, number_color = "black", fontsize = 10, fontsize_number = 5, annotation_col = annot_list_hm, annotation_colors = annot_col_hm)
Version | Author | Date |
---|---|---|
a07d5bc | emmapfort | 2025-03-19 |
pheatmap(fC_Matrix_Full_cpm_filter_spearmancor, border_color = "black", legend = TRUE, angle_col = 90, display_numbers = FALSE, number_color = "black", fontsize = 10, fontsize_number = 5, annotation_col = annot_list_hm, annotation_colors = annot_col_hm)
Version | Author | Date |
---|---|---|
a07d5bc | emmapfort | 2025-03-19 |
sessionInfo()
R version 4.4.2 (2024-10-31 ucrt)
Platform: x86_64-w64-mingw32/x64
Running under: Windows 11 x64 (build 22000)
Matrix products: default
locale:
[1] LC_COLLATE=English_United States.utf8
[2] LC_CTYPE=English_United States.utf8
[3] LC_MONETARY=English_United States.utf8
[4] LC_NUMERIC=C
[5] LC_TIME=English_United States.utf8
time zone: America/Chicago
tzcode source: internal
attached base packages:
[1] stats4 stats graphics grDevices utils datasets methods
[8] base
other attached packages:
[1] Rfast_2.1.5.1 RcppParallel_5.1.10
[3] zigg_0.0.2 Rcpp_1.0.13-1
[5] RUVSeq_1.40.0 EDASeq_2.40.0
[7] ShortRead_1.64.0 GenomicAlignments_1.42.0
[9] SummarizedExperiment_1.36.0 MatrixGenerics_1.18.1
[11] matrixStats_1.4.1 Rsamtools_2.22.0
[13] GenomicRanges_1.58.0 Biostrings_2.74.0
[15] GenomeInfoDb_1.42.3 XVector_0.46.0
[17] IRanges_2.40.0 S4Vectors_0.44.0
[19] BiocParallel_1.40.0 biomaRt_2.62.1
[21] RColorBrewer_1.1-3 Cormotif_1.52.0
[23] affy_1.84.0 Biobase_2.66.0
[25] BiocGenerics_0.52.0 PCAtools_2.18.0
[27] ggfortify_0.4.17 pheatmap_1.0.12
[29] edgeR_4.4.0 limma_3.62.1
[31] readxl_1.4.3 edgebundleR_0.1.4
[33] lubridate_1.9.3 forcats_1.0.0
[35] stringr_1.5.1 dplyr_1.1.4
[37] purrr_1.0.2 readr_2.1.5
[39] tidyr_1.3.1 tidyverse_2.0.0
[41] tibble_3.2.1 hrbrthemes_0.8.7
[43] reshape2_1.4.4 ggrepel_0.9.6
[45] ggplot2_3.5.1 workflowr_1.7.1
loaded via a namespace (and not attached):
[1] later_1.4.1 BiocIO_1.16.0
[3] bitops_1.0-9 filelock_1.0.3
[5] R.oo_1.27.0 cellranger_1.1.0
[7] preprocessCore_1.68.0 XML_3.99-0.18
[9] lifecycle_1.0.4 httr2_1.1.0
[11] pwalign_1.2.0 rprojroot_2.0.4
[13] vroom_1.6.5 MASS_7.3-61
[15] processx_3.8.5 lattice_0.22-6
[17] magrittr_2.0.3 sass_0.4.9
[19] rmarkdown_2.29 jquerylib_0.1.4
[21] yaml_2.3.10 httpuv_1.6.15
[23] cowplot_1.1.3 DBI_1.2.3
[25] abind_1.4-8 zlibbioc_1.52.0
[27] R.utils_2.12.3 RCurl_1.98-1.16
[29] rappdirs_0.3.3 git2r_0.35.0
[31] gdtools_0.4.1 GenomeInfoDbData_1.2.13
[33] irlba_2.3.5.1 dqrng_0.4.1
[35] DelayedMatrixStats_1.28.1 codetools_0.2-20
[37] DelayedArray_0.32.0 xml2_1.3.6
[39] tidyselect_1.2.1 farver_2.1.2
[41] UCSC.utils_1.2.0 ScaledMatrix_1.14.0
[43] BiocFileCache_2.14.0 jsonlite_1.8.9
[45] systemfonts_1.1.0 tools_4.4.2
[47] progress_1.2.3 glue_1.8.0
[49] gridExtra_2.3 Rttf2pt1_1.3.12
[51] SparseArray_1.6.0 xfun_0.49
[53] withr_3.0.2 BiocManager_1.30.25
[55] fastmap_1.2.0 latticeExtra_0.6-30
[57] callr_3.7.6 digest_0.6.37
[59] rsvd_1.0.5 timechange_0.3.0
[61] R6_2.6.1 mime_0.12
[63] colorspace_2.1-1 jpeg_0.1-10
[65] RSQLite_2.3.8 R.methodsS3_1.8.2
[67] generics_0.1.3 fontLiberation_0.1.0
[69] rtracklayer_1.66.0 prettyunits_1.2.0
[71] httr_1.4.7 htmlwidgets_1.6.4
[73] S4Arrays_1.6.0 whisker_0.4.1
[75] pkgconfig_2.0.3 gtable_0.3.6
[77] blob_1.2.4 hwriter_1.3.2.1
[79] htmltools_0.5.8.1 fontBitstreamVera_0.1.1
[81] scales_1.3.0 png_0.1-8
[83] knitr_1.49 rstudioapi_0.17.1
[85] tzdb_0.4.0 rjson_0.2.23
[87] curl_6.0.1 cachem_1.1.0
[89] parallel_4.4.2 extrafont_0.19
[91] AnnotationDbi_1.68.0 restfulr_0.0.15
[93] pillar_1.10.1 grid_4.4.2
[95] vctrs_0.6.5 promises_1.3.2
[97] BiocSingular_1.22.0 dbplyr_2.5.0
[99] beachmat_2.22.0 xtable_1.8-4
[101] extrafontdb_1.0 evaluate_1.0.3
[103] GenomicFeatures_1.58.0 cli_3.6.3
[105] locfit_1.5-9.10 compiler_4.4.2
[107] rlang_1.1.4 crayon_1.5.3
[109] labeling_0.4.3 aroma.light_3.36.0
[111] interp_1.1-6 ps_1.8.1
[113] getPass_0.2-4 plyr_1.8.9
[115] fs_1.6.5 stringi_1.8.4
[117] deldir_2.0-4 munsell_0.5.1
[119] fontquiver_0.2.1 Matrix_1.7-1
[121] hms_1.1.3 sparseMatrixStats_1.18.0
[123] bit64_4.5.2 KEGGREST_1.46.0
[125] statmod_1.5.0 shiny_1.10.0
[127] igraph_2.1.1 memoise_2.0.1
[129] affyio_1.76.0 bslib_0.9.0
[131] bit_4.5.0