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Rmd 9a91909 Jason Willwerscheid 2020-08-07 workflowr::wflow_publish(“analysis/pm1_priors7.Rmd”) 
suppressMessages({
  library(flashier)
  library(drift.alpha)
  library(tidyverse)
})

The purpose of this analysis is to experiment with the family of “three-pointmass priors” with mass on \(-a\), \(0\), and \(b\). I use the balanced tree from the previous analysis.

set.seed(666)

p <- 10000
resid_sd <- 0.1

# Define tree by mean branch length at each depth:
branch_means <- rep(1, 4)
branch_sds <- rep(0, 4)
depth <- length(branch_means)
npop_pure <- 2^(depth - 1)

# Define admixtures by admixture proportions:
admix_pops <- matrix(nrow = 0, ncol = 0)
npop_admix <- ncol(admix_pops)

npop <- npop_pure + npop_admix

n <- sample(30:100, npop, replace = TRUE)
# n <- rep(50, npop)
K <- 2^depth - 1

FF <- matrix(nrow = p, ncol = K)
k <- 1
for (d in 1:depth) {
  for (i in 1:(2^(d - 1))) {
    FF[, k] <- rnorm(p, sd = branch_means[d] + rnorm(1, sd = branch_sds[d]))
    k <- k + 1
  }
}

tree_mat <- matrix(0, nrow = npop_pure, ncol = K)
k <- 1
for (d in 1:depth) {
  size <- 2^(depth - d)
  for (i in 1:(2^(d - 1))) {
    tree_mat[((i - 1) * size + 1):(i * size), k] <- 1
    k <- k + 1
  }
}

pop_means <- FF %*% t(tree_mat)
if (npop_admix > 0) {
  pop_means <- cbind(pop_means, pop_means %*% admix_pops)
}

Y <- NULL
for (i in 1:npop) {
  Y <- rbind(Y, matrix(pop_means[, i], nrow = n[i], ncol = p, byrow = TRUE))
}
Y <- Y + rnorm(sum(n) * p, sd = resid_sd)

plot_dr <- function(dr) {
  sd <- sqrt(dr$prior_s2)
  L <- dr$EL
  LDsqrt <- L %*% diag(sd)
  K <- ncol(LDsqrt)
  plot_loadings(LDsqrt[,1:K], rep(letters[1:npop], n)) +
    scale_color_brewer(palette="Set3")
}

threepm.fn = function(x, s, g_init, fix_g, output, ...) {
  if (is.null(g_init)) {
    nllik_given_ab <- function(par) {
      g_init <- ashr::unimix(rep(1/3, 3), a = c(-par[1], 0, par[2]), b = c(-par[1], 0, par[2]))
      ebnm_res <- ebnm::ebnm_ash(x, s, g_init = g_init)
      return(-ebnm_res$log_likelihood)
    }
    opt_res <- optim(
      par = c(1, 1), 
      fn = nllik_given_ab, 
      method = "L-BFGS-B", 
      lower = c(0, 0), 
      upper = c(Inf, Inf)
    )
    par <- opt_res$par
    g_init <- ashr::unimix(rep(1/3, 3), a = c(-par[1], 0, par[2]), b = c(-par[1], 0, par[2]))
  }
  
  return(flashier:::ebnm.nowarn(x = x,
                                s = s,
                                g_init = g_init,
                                fix_g = fix_g,
                                output = output,
                                prior_family = "ash",
                                prior = c(1, 1, 1),
                                ...))
}

prior.threepm = function(...) {
  return(as.prior(sign = 0, ebnm.fn = function(x, s, g_init, fix_g, output) {
    threepm.fn(x, s, g_init, fix_g, output, ...)
  }))
}

flexpm.fn = function(x, s, g_init, fix_g, output, ...) {
  if (is.null(g_init)) {
    nllik_given_ab <- function(par) {
      g_init <- ashr::unimix(rep(1/4, 4), a = c(-par[1], 0, par[2], -par[1]), b = c(-par[1], 0, par[2], par[2]))
      ebnm_res <- ebnm::ebnm_ash(x, s, g_init = g_init)
      return(-ebnm_res$log_likelihood)
    }
    opt_res <- optim(
      par = c(1, 1), 
      fn = nllik_given_ab, 
      method = "L-BFGS-B", 
      lower = c(0, 0), 
      upper = c(Inf, Inf)
    )
    par <- opt_res$par
    g_init <- ashr::unimix(rep(1/4, 4), a = c(-par[1], 0, par[2], -par[1]), b = c(-par[1], 0, par[2], par[2]))
  }
  
  return(flashier:::ebnm.nowarn(x = x,
                                s = s,
                                g_init = g_init,
                                fix_g = fix_g,
                                output = output,
                                prior_family = "ash",
                                prior = c(1, 1, 1, 1),
                                ...))
}

prior.flexpm = function(...) {
  return(as.prior(sign = 0, ebnm.fn = function(x, s, g_init, fix_g, output) {
    threepm.fn(x, s, g_init, fix_g, output, ...)
  }))
}

init.mean.factor <- function(resids, zero.idx) {
  u <- matrix(1, nrow = nrow(resids), ncol = 1)
  u[zero.idx, 1] <- 0
  v <- t(solve(crossprod(u), crossprod(u, resids)))
  return(list(u, v))
}

init.split.factor <- function(resids, zero.idx) {
  svd.res <- svd(resids, nu = 1, nv = 1)
  u <- svd.res$u
  u[zero.idx] <- 0
  u <- matrix(sign(u), ncol = 1)
  v <- t(solve(crossprod(u), crossprod(u, resids)))
  return(list(u, v))
}

Unlike in the previous analysis, flash finds the tree:

fl <- flash.init(Y) %>%
  flash.set.verbose(0) %>%
  flash.init.factors(EF = init.mean.factor(Y, NULL), 
                     prior.family = c(prior.threepm(), prior.normal())) %>%
  flash.fix.loadings(kset = 1, mode = 1L) %>%
  flash.backfit() %>%
  flash.add.greedy(Kmax = npop_pure - 1, 
                   prior.family = c(prior.threepm(), prior.normal()))

plot_dr(init_from_flash(fl))

If, however, I add in some admixtures, the splits are all jumbled:

set.seed(666)

p <- 10000
resid_sd <- 0.1

# Define tree by mean branch length at each depth:
branch_means <- rep(1, 4)
branch_sds <- rep(0, 4)
depth <- length(branch_means)
npop_pure <- 2^(depth - 1)

# Define admixtures by admixture proportions:
admix_pops <- cbind(c(0, 0, 0, 0.4, 0.6, 0, 0, 0),
                   c(0, 0.15, 0.35, 0.5, 0, 0, 0, 0))
npop_admix <- ncol(admix_pops)

npop <- npop_pure + npop_admix

n <- sample(30:100, npop, replace = TRUE)
# n <- rep(50, npop)
K <- 2^depth - 1

FF <- matrix(nrow = p, ncol = K)
k <- 1
for (d in 1:depth) {
  for (i in 1:(2^(d - 1))) {
    FF[, k] <- rnorm(p, sd = branch_means[d] + rnorm(1, sd = branch_sds[d]))
    k <- k + 1
  }
}

tree_mat <- matrix(0, nrow = npop_pure, ncol = K)
k <- 1
for (d in 1:depth) {
  size <- 2^(depth - d)
  for (i in 1:(2^(d - 1))) {
    tree_mat[((i - 1) * size + 1):(i * size), k] <- 1
    k <- k + 1
  }
}

pop_means <- FF %*% t(tree_mat)
if (npop_admix > 0) {
  pop_means <- cbind(pop_means, pop_means %*% admix_pops)
}

Y <- NULL
for (i in 1:npop) {
  Y <- rbind(Y, matrix(pop_means[, i], nrow = n[i], ncol = p, byrow = TRUE))
}
Y <- Y + rnorm(sum(n) * p, sd = resid_sd)

fl <- flash.init(Y) %>%
  flash.set.verbose(0) %>%
  flash.init.factors(EF = init.mean.factor(Y, NULL), 
                     prior.family = c(prior.threepm(), prior.normal())) %>%
  flash.fix.loadings(kset = 1, mode = 1L) %>%
  flash.backfit() %>%
  flash.add.greedy(Kmax = npop_pure - 1, 
                   prior.family = c(prior.threepm(), prior.normal()))

plot_dr(init_from_flash(fl))



sessionInfo()
#> R version 3.5.3 (2019-03-11)
#> Platform: x86_64-apple-darwin15.6.0 (64-bit)
#> Running under: macOS Mojave 10.14.6
#> 
#> Matrix products: default
#> BLAS: /Library/Frameworks/R.framework/Versions/3.5/Resources/lib/libRblas.0.dylib
#> LAPACK: /Library/Frameworks/R.framework/Versions/3.5/Resources/lib/libRlapack.dylib
#> 
#> locale:
#> [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
#> 
#> attached base packages:
#> [1] stats     graphics  grDevices utils     datasets  methods   base     
#> 
#> other attached packages:
#>  [1] forcats_0.4.0      stringr_1.4.0      dplyr_0.8.0.1     
#>  [4] purrr_0.3.2        readr_1.3.1        tidyr_0.8.3       
#>  [7] tibble_2.1.1       ggplot2_3.2.0      tidyverse_1.2.1   
#> [10] drift.alpha_0.0.10 flashier_0.2.7    
#> 
#> loaded via a namespace (and not attached):
#>  [1] Rcpp_1.0.4.6       lubridate_1.7.4    invgamma_1.1      
#>  [4] lattice_0.20-38    assertthat_0.2.1   rprojroot_1.3-2   
#>  [7] digest_0.6.18      truncnorm_1.0-8    R6_2.4.0          
#> [10] cellranger_1.1.0   plyr_1.8.4         backports_1.1.3   
#> [13] evaluate_0.13      httr_1.4.0         pillar_1.3.1      
#> [16] rlang_0.4.2        lazyeval_0.2.2     readxl_1.3.1      
#> [19] rstudioapi_0.10    ebnm_0.1-21        irlba_2.3.3       
#> [22] whisker_0.3-2      Matrix_1.2-15      rmarkdown_1.12    
#> [25] labeling_0.3       munsell_0.5.0      mixsqp_0.3-40     
#> [28] broom_0.5.1        compiler_3.5.3     modelr_0.1.5      
#> [31] xfun_0.6           pkgconfig_2.0.2    SQUAREM_2017.10-1 
#> [34] htmltools_0.3.6    tidyselect_0.2.5   workflowr_1.2.0   
#> [37] withr_2.1.2        crayon_1.3.4       grid_3.5.3        
#> [40] nlme_3.1-137       jsonlite_1.6       gtable_0.3.0      
#> [43] git2r_0.25.2       magrittr_1.5       scales_1.0.0      
#> [46] cli_1.1.0          stringi_1.4.3      reshape2_1.4.3    
#> [49] fs_1.2.7           xml2_1.2.0         generics_0.0.2    
#> [52] RColorBrewer_1.1-2 tools_3.5.3        glue_1.3.1        
#> [55] hms_0.4.2          parallel_3.5.3     yaml_2.2.0        
#> [58] colorspace_1.4-1   ashr_2.2-51        rvest_0.3.4       
#> [61] knitr_1.22         haven_2.1.1