Last updated: 2021-01-18

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Rmd c8feb2d lukeholman 2020-11-30 Same page with Martin

Load R packages

# it was slightly harder to install the showtext package. On Mac, I did this:
# installed 'homebrew' using Terminal: ruby -e "$(curl -fsSL https://raw.githubusercontent.com/Homebrew/install/master/install)" 
# installed 'libpng' using Terminal: brew install libpng
# installed 'showtext' in R using: devtools::install_github("yixuan/showtext")  

library(tidyverse)
library(gridExtra)
library(grid)
library(brms)
library(RColorBrewer)
library(glue)
library(kableExtra)
library(tidybayes)
library(bayestestR)
library(MuMIn)
library(glue)
library(ggridges)
library(future)
library(future.apply)
library(GGally)
library(DT)
library(showtext)

library(knitrhooks) # install with devtools::install_github("nathaneastwood/knitrhooks")

output_max_height() # a knitrhook option
options(stringsAsFactors = FALSE)

# set up nice font for figure
nice_font <- "Lora"
font_add_google(name = nice_font, family = nice_font, regular.wt = 400, bold.wt = 700)
showtext_auto()

# Define function for the inverse logit
inv_logit <- function(x) 1 / (1 + exp(-x))

Inspect the raw data

This analysis set out to test whether sexual selection treatment had an effect on respiration in the flies. The variables in the raw data are as follows:

  • SELECTION The selection treatment of the focal triad of flies (monandry or polyandry)
  • SEX The sex of the focal triad of flies (male or female)
  • LINE The replicate selection line (M1-4 and P1-4)
  • SAMPLE Grouping variable that identifies the specific triad of flies (there are three measurements of each for all response variables except body weight). The have names like M1T1F (i.e. the first triad of females from line M1)
  • CYCLE The respirometry cycle (three measurements were taken, with cycle I being the first and cycle III the last)
  • VO2 The amount of O\(_2\) produced over the cycle
  • VCO2 The amount of CO\(_2\) consumed over the cycle
  • RQ The respiratory quotient, i.e. VCO2 / VO2
  • ACTIVITY The amount of locomotor activity by the triad of flies over the focal cycle; measured by infrared light
  • BODYMASS The mass/weight of the triad of flies, measured to the nearest 0.1mg.

We expect body weight and activity levels to vary between the sexes and potentially between treatments. In turn, we expect these two ‘mediator variables’ to co-vary with respiratory flux, e.g. because larger flies have more respiring tissue, and because more active flies would have more active metabolism. There might also be weight- and activity-independent effects of sex and selection treatment on respiration, e.g. because of differences in resting metabolic rate.

Raw numbers

respiration <- read_csv("data/2.metabolic_rates.csv") %>%
  rename(SELECTION = `?SELECTION`,
         BODYMASS = BODY_WEIGHT)

my_data_table <- function(df){
  datatable(
    df, rownames=FALSE,
    autoHideNavigation = TRUE,
    extensions = c("Scroller",  "Buttons"),
    options = list(
      dom = 'Bfrtip',
      deferRender=TRUE,
      scrollX=TRUE, scrollY=400,
      scrollCollapse=TRUE,
      buttons = 
        list('csv', list(
          extend = 'pdf',
          pageSize = 'A4',
          orientation = 'landscape',
          filename = 'Dpseudo_respiration')),
      pageLength = 50
    )
  )
}

respiration %>%
  mutate_if(is.numeric, ~ format(round(.x, 3), nsmall = 3)) %>%
  my_data_table()

Plot of correlations between variables

cols <- c("M females" = "pink", 
          "P females" = "red", 
          "M males" = "skyblue", 
          "P males" = "blue")

modified_densityDiag <- function(data, mapping, ...) {
  ggally_densityDiag(data, mapping, colour = "grey10", ...) + 
    scale_fill_manual(values = cols) + 
  scale_x_continuous(guide = guide_axis(check.overlap = TRUE))
}

modified_points <- function(data, mapping, ...) {
  ggally_points(data, mapping, pch = 21, colour = "grey10", ...) +
    scale_fill_manual(values = cols) + 
    scale_x_continuous(guide = guide_axis(check.overlap = TRUE))
}

modified_facetdensity <- function(data, mapping, ...) {
  ggally_facetdensity(data, mapping, ...) + 
    scale_colour_manual(values = cols)
}

modified_box_no_facet <- function(data, mapping, ...) {
  ggally_box_no_facet(data, mapping, colour = "grey10", ...) +
    scale_fill_manual(values = cols)
}

respiration_pairs_plot <- respiration %>% 
  select(SEX, SELECTION, CYCLE, VO2, VCO2, RQ, ACTIVITY, BODYMASS) %>%
  mutate(VO2 = VO2 * 1000, 
         VCO2 = VCO2 * 1000, 
         BODYMASS = BODYMASS * 1000, 
         ACTIVITY = ACTIVITY * 100) %>%
  mutate(SEX = factor(ifelse(SEX == "M", "males", "females")),
         SELECTION = factor(ifelse(SELECTION == "Mono", "M", "P")),
         `Sex and treatment` = factor(paste(SELECTION, SEX), c("M males", "P males", "M females", "P females"))) %>%
  mutate_if(is.numeric, ~ as.numeric(scale(.x))) %>%
  select(-SEX, -SELECTION) %>%
  rename(Cycle = CYCLE, Activity = ACTIVITY, `Body mass` = BODYMASS) %>% 
  select(VO2, VCO2, RQ, Activity, `Body mass`, `Sex and treatment`, Cycle) %>%
  ggpairs(aes(colour = `Sex and treatment`, fill = `Sex and treatment`),
          diag = list(continuous = wrap(modified_densityDiag, alpha = 0.7),
                      discrete = wrap("blank")),
          lower = list(continuous = wrap(modified_points, alpha = 0.7, size = 1.1), 
                       discrete = wrap("blank"),
                       combo = wrap(modified_box_no_facet, alpha = 0.7)),
          upper = list(continuous = wrap(modified_points, alpha = 0.7, size = 1.1),
                       discrete = wrap("blank"),
                       combo = wrap(modified_box_no_facet, alpha = 0.7, size = 0.5))) 

respiration_pairs_plot %>% ggsave(filename = "figures/respiration_pairs_plot.pdf", height = 10, width = 10)
respiration_pairs_plot

Version Author Date
47bc217 lukeholman 2020-12-18
a14c378 lukeholman 2020-12-18

Figure XX: Boxplots, scatterplots, and density plots illustrating the means, variances and covariances of the explanatory variables (Treatment, Sex, and Cycle) and the five response variables. The data are coloured by treatment (red: polyandry, blue: monogamy). The plot shows the raw data in their original units, namely the number of mm of O\(_2\) consumed or CO\(_2\) produced, the % time spent active, and the body mass in milligrams; the respiratory quotient (RQ) is a unitless ratio. Note that the true value of RQ must lie within the range of 0.7-1.0 because of the chemistry of respiration, but estimates of RQ can lie outside this range due to sampling variance and measurement error for O\(_2\) and CO\(_2\).

Group means

Body weight (per triad)

se <- function(x) sd(x) / sqrt(length(x))
respiration %>% 
  select(SEX, SELECTION, BODYMASS, SAMPLE) %>% 
  distinct(SAMPLE, .keep_all = TRUE) %>%
  group_by(SEX, SELECTION) %>% 
  summarise(`Mean weight per fly (mg)` = mean(BODYMASS * 1000 / 3), 
            SE = se(BODYMASS * 1000 / 3), 
            n = n()) %>% 
  kable(digits = 2) %>% kable_styling(full_width = FALSE)
SEX SELECTION Mean weight per fly (mg) SE n
F Mono 0.40 0.02 12
F Poly 0.47 0.04 12
M Mono 0.27 0.02 12
M Poly 0.29 0.02 12

Mean activity level

respiration %>% 
  select(SEX, SELECTION, ACTIVITY, CYCLE, SAMPLE) %>%
  distinct(SAMPLE, CYCLE, .keep_all = TRUE) %>%
  group_by(SEX, SELECTION, CYCLE) %>% 
  summarise(`Mean activity level` = mean(ACTIVITY * 1000 / 3), 
            SE = se(ACTIVITY * 1000 / 3), 
            n = n()) %>% 
  kable(digits = 2) %>% kable_styling(full_width = FALSE)
SEX SELECTION CYCLE Mean activity level SE n
F Mono I 3.63 0.80 12
F Mono II 6.05 1.19 12
F Mono III 6.54 1.49 12
F Poly I 14.16 2.95 12
F Poly II 16.24 3.14 12
F Poly III 15.63 2.78 12
M Mono I 9.11 1.96 12
M Mono II 6.16 1.19 12
M Mono III 4.84 0.96 12
M Poly I 16.62 1.27 12
M Poly II 18.02 1.10 12
M Poly III 17.45 1.71 12

Mean VO\(_2\)

respiration %>% 
  select(SEX, SELECTION, VO2, CYCLE, SAMPLE) %>%
  distinct(SAMPLE, CYCLE, .keep_all = TRUE) %>% 
  group_by(SEX, SELECTION, CYCLE) %>% 
  summarise(`Mean VO2` = mean(VO2 * 1000), 
            SE = se(VO2 * 1000), 
            n = n()) %>% 
  kable(digits = 2) %>% kable_styling(full_width = FALSE)
SEX SELECTION CYCLE Mean VO2 SE n
F Mono I 7.75 0.57 12
F Mono II 6.42 0.28 12
F Mono III 5.96 0.48 12
F Poly I 8.43 0.54 12
F Poly II 7.98 0.62 12
F Poly III 6.73 0.64 12
M Mono I 6.37 0.61 12
M Mono II 5.16 0.33 12
M Mono III 3.60 0.37 12
M Poly I 8.11 0.45 12
M Poly II 7.44 0.49 12
M Poly III 6.37 0.44 12

Mean VCO\(_2\)

respiration %>% 
  select(SEX, SELECTION, VCO2, CYCLE, SAMPLE) %>%
  distinct(SAMPLE, CYCLE, .keep_all = TRUE) %>%
  group_by(SEX, SELECTION, CYCLE) %>% 
  summarise(`Mean VCO2` = mean(VCO2 * 1000), 
            SE = se(VCO2 * 1000), 
            n = n()) %>% 
  kable(digits = 2) %>% kable_styling(full_width = FALSE)
SEX SELECTION CYCLE Mean VCO2 SE n
F Mono I 6.75 0.47 12
F Mono II 5.66 0.28 12
F Mono III 4.98 0.41 12
F Poly I 7.20 0.39 12
F Poly II 7.13 0.43 12
F Poly III 6.18 0.43 12
M Mono I 5.66 0.42 12
M Mono II 4.62 0.16 12
M Mono III 3.42 0.17 12
M Poly I 7.32 0.57 12
M Poly II 6.37 0.46 12
M Poly III 5.56 0.32 12

Mean RQ

respiration %>% 
  select(SEX, SELECTION, RQ, CYCLE, SAMPLE) %>%
  distinct(SAMPLE, CYCLE, .keep_all = TRUE) %>%
  group_by(SEX, SELECTION, CYCLE) %>% 
  summarise(`Mean VO2` = mean(RQ), 
            SE = se(RQ), 
            n = n()) %>% 
  kable(digits = 2) %>% kable_styling(full_width = FALSE)
SEX SELECTION CYCLE Mean VO2 SE n
F Mono I 0.88 0.03 12
F Mono II 0.88 0.02 12
F Mono III 0.84 0.02 12
F Poly I 0.86 0.02 12
F Poly II 0.91 0.02 12
F Poly III 0.95 0.04 12
M Mono I 0.92 0.04 12
M Mono II 0.92 0.04 12
M Mono III 1.01 0.06 12
M Poly I 0.90 0.03 12
M Poly II 0.85 0.01 12
M Poly III 0.88 0.03 12

Draw the causal diagram

DiagrammeR::grViz('digraph {

graph [layout = dot, rankdir = LR]

# define the global styles of the nodes. We can override these in box if we wish
node [shape = rectangle, style = filled, fillcolor = Linen]

"Metabolic\nrate" [shape = oval, fillcolor = Beige]
"Metabolic\nsubstrate" [shape = oval, fillcolor = Beige]
"Other factors\n(e.g. physiology)" [shape = oval, fillcolor = Beige]

# edge definitions with the node IDs
"Mating system\ntreatment (M vs P)" -> {"Other factors\n(e.g. physiology)", "Body mass" , "Activity"} -> {"Metabolic\nrate", "Metabolic\nsubstrate"} 

{"Metabolic\nrate"} -> {"O\u2082 consumption", "CO\u2082 production"}
{"O\u2082 consumption", "CO\u2082 production"} -> "Respiratory\nquotient (RQ)"
{"Metabolic\nsubstrate"} -> "Respiratory\nquotient (RQ)"
}')



Figure XX: Directed acyclic graph (DAG) showing the key causal relationships that we hypothesised a priori between the measured variables (squares) and latent variables (ovals). This DAG motivated the Bayesian structural equation model discussed in the Methods and Results, which attempts to decompose the effects of treatment on respiration (measured via O2 and CO2 flux, and their ratio, RQ) into paths that travel via body mass, activity, or other unmeasured factors such as physiology.

Fit Bayesian structural equation model (SEM) via brms

Scale the data

Here, we mean-center and scale body mass and activity. We also multiply VO2 and VCO2 by 1000, such that their units (and thus the associated regression coefficients) are not too small (and have means closer to those expected by the brms default intercept priors).

The reason we do not mean-center and scale VO2 and VCO2 is that the model below relates them to each other in terms of the respiratory quotient, RQ, which would be more complex if they had been converted to standard deviations.

scaled_data <- respiration %>%
  mutate(VO2 = VO2 * 1000, 
         VCO2 = VCO2 * 1000,
         BODYMASS = as.numeric(scale(BODYMASS)),
         ACTIVITY = as.numeric(scale(ACTIVITY))) %>% 
  select(-RQ) 

scaled_data %>%
  mutate_if(is.numeric, ~ format(round(.x, 3), nsmall = 3)) %>%
  my_data_table()

Define the SEM’s sub-models

Here, we write out all the formulae of the sub-models in the SEM. There is a sub-model for oxygen consumption (VO2) and another for CO2 production (VCO2) which depends on the parameter RQ (the respiratory quotient, i.e. VCO2 / VO2); there are also sub-models for body mass (BODYMASS) and activity level (ACTIVITY).

In short, we assume that VO2, RQ, BODYMASS, and ACTIVITY are affected by the predictor variables related to the experimental design. These variables are SELECTION (i.e. M vs P treatment), SEX (Male or Female), CYCLE (I, II, or III: this refers to the first, second, and third measurement of O2 and CO2 for each triad of flies), LINE (8 levels, one for each of the four independent replicates of the M an P treatments), and SAMPLE (a random intercept that groups the three replicate measures of each triad of flies across the three cycles). Unlike the other response variables, VCO2 is predicted from the estimated values of VO2 and RQ as VO2 RQ, and thus is related to the predictor variables only indirectly (the choice to make VCO2 dependent rather than VO2 dependant on RQ is arbitrary, and does not affect the results).

The brms notation for the line random effects ((SELECTION | p | LINE)) means that the model fits a random intercept for each of the 8 lines, and that these random intercepts are (potentially) correlated across each of the response variables. Furthermore, the model fits a random slope for SELECTION, which allows the treatment effect to vary across the replicate lines.

VO2

Formula: VO2 ~ SELECTION * SEX * CYCLE + BODYMASS + ACTIVITY + (SELECTION | p | LINE) + (1 | SAMPLE)

This formula allows for effects on VO2 of sex, selection and cycle (and all 2- and 3-way interactions). The model also allows for linear effects of BODYMASS and ACTIVITY on VO2. Furthermore the model assumes there may be variation in VO2 within and between each triad of flies, and between each replicate selection line (preventing pseudo-replication by properly accounting for our experimental design).

VCO2 (as determined by the estimated parameter RQ)

Formulae (2-part model, see vignette("brms_nonlinear")):

VCO2 ~ VO2 * (0.7 + 0.3 * inv_logit(RQ))

RQ ~ SELECTION * SEX * CYCLE + BODYMASS + ACTIVITY + (SELECTION | p | LINE) + (1 | SAMPLE)

VCO2 is assumed to depend on the value of VO2 from the same measurement, multiplied by RQ. We use a modified inverse logit function that forces RQ to lie 0.7 and 1 (this must be the case, given the chemistry of respiration, and building this information into the model should yield more precise estimates of the model’s parameters). In turn, RQ is predicted by the same set of predictors as for VO2.

Body mass

BODYMASS | subset(body_subset) ~ SELECTION * SEX + (SELECTION | p | LINE)

The model fits the selection line treatment and sex as fixed effects, as well as the random intercept for line. There is not SAMPLE random effect, since the body mass of each triad of flies was measured only once (unlike for the other response variables, which were each measured 3 times). The notation subset(body_subset) instructs brms to fit this model using only a sub-set of the data, because unfortunately the body mass data was not collected for every triad of flies due to an experimental error (leaving our the subset term would discard all rows of the data for which body size was not measured, wasting the data for the other response variables).

Activity level

ACTIVITY ~ SELECTION * SEX * CYCLE + (SELECTION | p | LINE) + (1 | SAMPLE)

The model fits the selection line treatment and sex as fixed effects, as well as crossed random intercepts for line and sample.

brms_data <- scaled_data %>%
  mutate(body_subset = CYCLE == "I")    

brms_formula <- 
  bf(VO2 ~ SELECTION * SEX * CYCLE +  # VO2 sub-model
       BODYMASS + ACTIVITY +  
       (SELECTION | p | LINE) + (1 | SAMPLE)) +
  
  bf(VCO2 ~ VO2 * (0.7 + 0.3 * inv_logit(RQ)),   # VCO2 and RQ sub-models
     RQ ~ SELECTION * SEX * CYCLE + 
       BODYMASS + ACTIVITY + 
       (SELECTION | p | LINE) + (1 | SAMPLE),
     nl = TRUE) +
  
  bf(BODYMASS | subset(body_subset) ~ SELECTION * SEX + # body mass sub-model
       (SELECTION | p | LINE)) +
  
  bf(ACTIVITY ~ SELECTION * SEX * CYCLE +   # activity sub-model
       (SELECTION | p | LINE) + (1 | SAMPLE)) +  
  
  set_rescor(FALSE) # cannot estimate residual correlations when using the subset() argument

brms_formula
VO2 ~ SELECTION * SEX * CYCLE + BODYMASS + ACTIVITY + (SELECTION | p | LINE) + (1 | SAMPLE) 
VCO2 ~ VO2 * (0.7 + 0.3 * inv_logit(RQ)) 
RQ ~ SELECTION * SEX * CYCLE + BODYMASS + ACTIVITY + (SELECTION | p | LINE) + (1 | SAMPLE)
BODYMASS | subset(body_subset) ~ SELECTION * SEX + (SELECTION | p | LINE) 
ACTIVITY ~ SELECTION * SEX * CYCLE + (SELECTION | p | LINE) + (1 | SAMPLE) 

Define Priors

We use set fairly tight Normal priors on all fixed effect parameters, which ‘regularises’ the estimates towards zero – this is conservative (because it ensures that a stronger signal in the data is needed to produce a given posterior effect size estimate), and it also helps the model to converge. Similarly, we set a somewhat conservative half-Cauchy prior (mean 0, scale 0.1) on the random effects for LINE (i.e. we consider large differences between lines – in terms of means and treatment effects – to be possible but improbable). We leave all other priors at the defaults used by brms. Note that the Normal priors are slightly wider in the model of dry weight, because we expect larger effect sizes of sex and treatment on dry weight than on the metabolite composition.

prior_SEM <- c(set_prior("normal(0, 1)", class = "b", resp = 'VO2'),
            set_prior("normal(0, 1)", class = "b", resp = "VCO2", nlpar = "RQ"),
            set_prior("normal(0, 1)", class = "b", resp = "BODYMASS"),
            set_prior("normal(0, 1)", class = "b", resp = "ACTIVITY"),
            
            set_prior("cauchy(0, 0.1)", class = "sd", resp = 'VO2', group = "LINE"),
            set_prior("cauchy(0, 0.1)", class = "sd", resp = "VCO2", nlpar = "RQ", group = "LINE"),
            set_prior("cauchy(0, 0.1)", class = "sd", resp = 'VO2', group = "SAMPLE"),
            set_prior("cauchy(0, 0.1)", class = "sd", resp = "VCO2", nlpar = "RQ", group = "SAMPLE"),
            set_prior("cauchy(0, 0.1)", class = "sd", resp = 'BODYMASS', group = "LINE"),
            set_prior("cauchy(0, 0.1)", class = "sd", resp = 'ACTIVITY', group = "LINE"),
            set_prior("cauchy(0, 0.1)", class = "sd", resp = 'ACTIVITY', group = "SAMPLE"))

prior_SEM
          prior class coef  group     resp dpar nlpar bound source
   normal(0, 1)     b                  VO2                    user
   normal(0, 1)     b                 VCO2         RQ         user
   normal(0, 1)     b             BODYMASS                    user
   normal(0, 1)     b             ACTIVITY                    user
 cauchy(0, 0.1)    sd        LINE      VO2                    user
 cauchy(0, 0.1)    sd        LINE     VCO2         RQ         user
 cauchy(0, 0.1)    sd      SAMPLE      VO2                    user
 cauchy(0, 0.1)    sd      SAMPLE     VCO2         RQ         user
 cauchy(0, 0.1)    sd        LINE BODYMASS                    user
 cauchy(0, 0.1)    sd        LINE ACTIVITY                    user
 cauchy(0, 0.1)    sd      SAMPLE ACTIVITY                    user

Run the model

The model is run over 4 chains with 7000 iterations each (with the first 3500 discarded as burn-in), for a total of 3500*4 = 14,000 posterior samples.

if(!file.exists("output/brms_SEM_respiration.rds")){
  brms_SEM_respiration <- brm(
    brms_formula, 
    data = brms_data, 
    iter = 7000, chains = 4, cores = 1,
    prior = prior_SEM,
    control = list(max_treedepth = 20, adapt_delta = 0.99))
  
  saveRDS(brms_SEM_respiration, "output/brms_SEM_respiration.rds")
} else brms_SEM_respiration <- readRDS("output/brms_SEM_respiration.rds")

Posterior predictive check of model fit

The plot below shows that the fitted model is able to produce posterior predictions that have a similar distribution to the original data, for each of the response variables, which is a necessary condition for the model to be used for statistical inference. Note that the fit produces less accurate predictions for body mass, reflecting the smaller number of data points for this response variable.

grid.arrange(
  pp_check(brms_SEM_respiration, resp = "VO2") + 
    ggtitle("VO2") + theme(legend.position = "none"),
  pp_check(brms_SEM_respiration, resp = "VCO2") + 
    ggtitle("VCO2") + theme(legend.position = "none"),
  pp_check(brms_SEM_respiration, resp = "BODYMASS") + 
    ggtitle("Body mass") + theme(legend.position = "none"),
  pp_check(brms_SEM_respiration, resp = "ACTIVITY") + 
    ggtitle("Activity level") + theme(legend.position = "none"),
  nrow = 1
)

Version Author Date
2791e70 lukeholman 2020-12-18
a14c378 lukeholman 2020-12-18
2fc158d lukeholman 2020-12-18

Table of model parameter estimates

Formatted table

This tables shows the fixed effects estimates for the treatment, sex, their interaction, as well as the slope associated with dry weight (where relevant), for each of the six response variables. The p column shows 1 - minus the “probability of direction”, i.e. the posterior probability that the reported sign of the estimate is correct given the data and the prior; subtracting this value from one gives a Bayesian equivalent of a one-sided p-value. For brevity, we have omitted all the parameter estimates involving the predictor variable line, as well as the estimates of residual (co)variance. Click the next tab to see a complete summary of the model and its output.

hypSEM <- left_join(
  fixef(brms_SEM_respiration) %>% as.data.frame() %>% rownames_to_column("Parameter"),
  bayestestR::p_direction(brms_SEM_respiration) %>% as.data.frame() %>% select(Parameter, pd) %>%
  mutate(Parameter = str_remove_all(Parameter, "b_"), Parameter = str_replace_all(Parameter, "[.]", ":")), 
  by = "Parameter"
) %>% mutate(Response = map_chr(str_split(Parameter, "_"), ~ .x[1]),
             Parameter = map(str_split(Parameter, "_"), ~ .x[length(.x)]),
             pd = 1 - pd) %>%
  select(Response, Parameter, everything()) %>% 
  rename(p = pd) %>%
  mutate(Parameter = str_replace_all(Parameter, "SELECTIONPoly", "Treatment (P)"),
         Parameter = str_replace_all(Parameter, "SEXM", "Sex (M)"),
         Parameter = str_replace_all(Parameter, "BODYMASS", "Body weight"),
         Parameter = str_replace_all(Parameter, "ACTIVITY", "Activity level"),
         Parameter = str_replace_all(Parameter, "CYCLEIII", "Cycle (III)"),
         Parameter = str_replace_all(Parameter, "CYCLEII", "Cycle (II)"),
         Parameter = str_replace_all(Parameter, ":", " x ")) %>%
  mutate(Response = str_replace_all(Response, "BODYMASS", "Body weight"),
         Response = str_replace_all(Response, "ACTIVITY", "Activity level"),
         Response = str_replace_all(Response, "VCO2", "Respiratory quotient (RQ)"),
         Response = factor(Response, c("VO2", "Respiratory quotient (RQ)", "Activity level", "Body weight"))) %>%
  mutate(` ` = ifelse(p < 0.05, "\\*", "")) %>%
  distinct() %>%
  arrange(Response) %>%
  select(-Response)

hypSEM  %>% 
  kable(digits = 3) %>% 
  kable_styling(full_width = FALSE) %>%
  group_rows("Oxygen consumption (VO2)", 1, 14) %>%
  group_rows("Respiratory quotient (RQ)", 15, 28) %>%
  group_rows("Activity level", 29, 40) %>%
  group_rows("Body weight", 41, 44) 
Parameter Estimate Est.Error Q2.5 Q97.5 p
Oxygen consumption (VO2)
Intercept 8.047 0.411 7.231 8.851 0.000 *
Treatment (P) -0.139 0.523 -1.135 0.905 0.389
Sex (M) -1.009 0.490 -1.967 -0.046 0.021 *
Cycle (II) -1.103 0.336 -1.754 -0.433 0.001 *
Cycle (III) -1.727 0.332 -2.361 -1.073 0.000 *
Body weight 0.300 0.235 -0.164 0.772 0.099
Activity level 1.006 0.166 0.677 1.332 0.000 *
Treatment (P) x Sex (M) 0.647 0.564 -0.471 1.737 0.125
Treatment (P) x Cycle (II) 0.309 0.442 -0.563 1.180 0.243
Treatment (P) x Cycle (III) -0.135 0.429 -0.974 0.717 0.380
Sex (M) x Cycle (II) 0.152 0.441 -0.721 1.005 0.367
Sex (M) x Cycle (III) -0.507 0.442 -1.376 0.365 0.127
Treatment (P) x Sex (M) x Cycle (II) -0.160 0.561 -1.265 0.940 0.389
Treatment (P) x Sex (M) x Cycle (III) 0.514 0.559 -0.591 1.609 0.176
Respiratory quotient (RQ)
Intercept -0.008 0.321 -0.618 0.636 0.484
Treatment (P) 0.297 0.447 -0.588 1.181 0.247
Sex (M) 0.480 0.429 -0.357 1.336 0.130
Cycle (II) 0.394 0.378 -0.340 1.155 0.146
Cycle (III) -0.107 0.379 -0.848 0.648 0.387
Body weight -0.013 0.182 -0.374 0.349 0.472
Activity level -0.224 0.155 -0.528 0.084 0.073
Treatment (P) x Sex (M) 0.134 0.502 -0.870 1.098 0.388
Treatment (P) x Cycle (II) 0.028 0.456 -0.883 0.924 0.474
Treatment (P) x Cycle (III) 0.571 0.480 -0.375 1.523 0.115
Sex (M) x Cycle (II) -0.528 0.521 -1.549 0.499 0.154
Sex (M) x Cycle (III) -0.029 0.574 -1.164 1.104 0.480
Treatment (P) x Sex (M) x Cycle (II) -0.467 0.606 -1.667 0.725 0.220
Treatment (P) x Sex (M) x Cycle (III) -0.815 0.652 -2.084 0.466 0.107
Activity level
Intercept -0.780 0.218 -1.210 -0.339 0.000 *
Treatment (P) 1.047 0.319 0.421 1.669 0.002 *
Sex (M) 0.457 0.283 -0.101 1.008 0.055
Cycle (II) 0.192 0.186 -0.173 0.555 0.149
Cycle (III) 0.238 0.188 -0.130 0.607 0.103
Treatment (P) x Sex (M) -0.065 0.383 -0.810 0.692 0.432
Treatment (P) x Cycle (II) 0.104 0.259 -0.397 0.620 0.346
Treatment (P) x Cycle (III) -0.012 0.259 -0.512 0.497 0.479
Sex (M) x Cycle (II) -0.480 0.258 -0.989 0.027 0.031 *
Sex (M) x Cycle (III) -0.676 0.258 -1.185 -0.161 0.005 *
Treatment (P) x Sex (M) x Cycle (II) 0.325 0.351 -0.364 1.010 0.179
Treatment (P) x Sex (M) x Cycle (III) 0.519 0.352 -0.166 1.198 0.072
Body weight
Intercept 0.348 0.250 -0.145 0.846 0.073
Treatment (P) 0.526 0.378 -0.234 1.253 0.078
Sex (M) -1.019 0.256 -1.521 -0.508 0.000 *
Treatment (P) x Sex (M) -0.444 0.353 -1.136 0.258 0.099

Complete output from summary.brmsfit()

  • ‘Group-Level Effects’ (also called random effects): This shows the (co)variances associated with the line-specific intercepts (which have names like sd(VO2_Intercept)) and slopes (e.g. sd(VO2_SELECTIONPoly)), as well as the correlations between these effects (e.g. cor(VO2_Intercept,VO2_SELECTIONPoly).
  • ‘Population-Level Effects:’ (also called fixed effects): These give the estimates of the intercept (i.e. for female M flies, in Cycle I) and the effects of treatment, sex, dry weight, and the various interaction terms, for each response variable.
  • ‘Family Specific Parameters’: This is the parameter sigma for the residual variance for each response variable

Note that the model has converged (Rhat = 1) and the posterior is adequately samples (high ESS values).

brms_SEM_respiration
 Family: MV(gaussian, gaussian, gaussian, gaussian) 
  Links: mu = identity; sigma = identity
         mu = identity; sigma = identity
         mu = identity; sigma = identity
         mu = identity; sigma = identity 
Formula: VO2 ~ SELECTION * SEX * CYCLE + BODYMASS + ACTIVITY + (SELECTION | p | LINE) + (1 | SAMPLE) 
         VCO2 ~ VO2 * (0.7 + 0.3 * inv_logit(RQ)) 
         RQ ~ SELECTION * SEX * CYCLE + BODYMASS + ACTIVITY + (SELECTION | p | LINE) + (1 | SAMPLE)
         BODYMASS | subset(body_subset) ~ SELECTION * SEX + (SELECTION | p | LINE) 
         ACTIVITY ~ SELECTION * SEX * CYCLE + (SELECTION | p | LINE) + (1 | SAMPLE) 
   Data: brms_data (Number of observations: 144) 
Samples: 4 chains, each with iter = 7000; warmup = 3500; thin = 1;
         total post-warmup samples = 14000

Group-Level Effects: 
~LINE (Number of levels: 8) 
                                                   Estimate Est.Error l-95% CI
sd(VO2_Intercept)                                      0.28      0.26     0.01
sd(VO2_SELECTIONPoly)                                  0.19      0.26     0.00
sd(VCO2_RQ_Intercept)                                  0.26      0.24     0.01
sd(VCO2_RQ_SELECTIONPoly)                              0.12      0.15     0.00
sd(BODYMASS_Intercept)                                 0.28      0.22     0.01
sd(BODYMASS_SELECTIONPoly)                             0.28      0.30     0.01
sd(ACTIVITY_Intercept)                                 0.10      0.10     0.00
sd(ACTIVITY_SELECTIONPoly)                             0.19      0.22     0.01
cor(VO2_Intercept,VO2_SELECTIONPoly)                   0.00      0.34    -0.64
cor(VO2_Intercept,VCO2_RQ_Intercept)                  -0.09      0.33    -0.67
cor(VO2_SELECTIONPoly,VCO2_RQ_Intercept)              -0.01      0.33    -0.64
cor(VO2_Intercept,VCO2_RQ_SELECTIONPoly)              -0.01      0.33    -0.64
cor(VO2_SELECTIONPoly,VCO2_RQ_SELECTIONPoly)          -0.00      0.33    -0.63
cor(VCO2_RQ_Intercept,VCO2_RQ_SELECTIONPoly)          -0.02      0.33    -0.65
cor(VO2_Intercept,BODYMASS_Intercept)                  0.01      0.33    -0.62
cor(VO2_SELECTIONPoly,BODYMASS_Intercept)             -0.05      0.33    -0.67
cor(VCO2_RQ_Intercept,BODYMASS_Intercept)             -0.01      0.32    -0.62
cor(VCO2_RQ_SELECTIONPoly,BODYMASS_Intercept)         -0.01      0.34    -0.63
cor(VO2_Intercept,BODYMASS_SELECTIONPoly)             -0.05      0.33    -0.65
cor(VO2_SELECTIONPoly,BODYMASS_SELECTIONPoly)         -0.04      0.33    -0.66
cor(VCO2_RQ_Intercept,BODYMASS_SELECTIONPoly)         -0.01      0.33    -0.63
cor(VCO2_RQ_SELECTIONPoly,BODYMASS_SELECTIONPoly)     -0.00      0.33    -0.63
cor(BODYMASS_Intercept,BODYMASS_SELECTIONPoly)         0.02      0.33    -0.61
cor(VO2_Intercept,ACTIVITY_Intercept)                  0.02      0.33    -0.62
cor(VO2_SELECTIONPoly,ACTIVITY_Intercept)              0.03      0.33    -0.61
cor(VCO2_RQ_Intercept,ACTIVITY_Intercept)              0.01      0.33    -0.62
cor(VCO2_RQ_SELECTIONPoly,ACTIVITY_Intercept)         -0.00      0.33    -0.63
cor(BODYMASS_Intercept,ACTIVITY_Intercept)            -0.07      0.33    -0.68
cor(BODYMASS_SELECTIONPoly,ACTIVITY_Intercept)        -0.05      0.33    -0.66
cor(VO2_Intercept,ACTIVITY_SELECTIONPoly)              0.05      0.34    -0.60
cor(VO2_SELECTIONPoly,ACTIVITY_SELECTIONPoly)          0.04      0.34    -0.61
cor(VCO2_RQ_Intercept,ACTIVITY_SELECTIONPoly)          0.00      0.33    -0.62
cor(VCO2_RQ_SELECTIONPoly,ACTIVITY_SELECTIONPoly)     -0.00      0.33    -0.64
cor(BODYMASS_Intercept,ACTIVITY_SELECTIONPoly)        -0.07      0.33    -0.68
cor(BODYMASS_SELECTIONPoly,ACTIVITY_SELECTIONPoly)    -0.06      0.33    -0.68
cor(ACTIVITY_Intercept,ACTIVITY_SELECTIONPoly)         0.01      0.33    -0.63
                                                   u-95% CI Rhat Bulk_ESS
sd(VO2_Intercept)                                      0.93 1.00     2908
sd(VO2_SELECTIONPoly)                                  0.90 1.00     7982
sd(VCO2_RQ_Intercept)                                  0.87 1.00     4358
sd(VCO2_RQ_SELECTIONPoly)                              0.50 1.00    15911
sd(BODYMASS_Intercept)                                 0.78 1.00     3783
sd(BODYMASS_SELECTIONPoly)                             1.04 1.00     5497
sd(ACTIVITY_Intercept)                                 0.36 1.00     6088
sd(ACTIVITY_SELECTIONPoly)                             0.72 1.00     4457
cor(VO2_Intercept,VO2_SELECTIONPoly)                   0.64 1.00    23872
cor(VO2_Intercept,VCO2_RQ_Intercept)                   0.56 1.00    14640
cor(VO2_SELECTIONPoly,VCO2_RQ_Intercept)               0.63 1.00    16067
cor(VO2_Intercept,VCO2_RQ_SELECTIONPoly)               0.63 1.00    30262
cor(VO2_SELECTIONPoly,VCO2_RQ_SELECTIONPoly)           0.64 1.00    21055
cor(VCO2_RQ_Intercept,VCO2_RQ_SELECTIONPoly)           0.62 1.00    17538
cor(VO2_Intercept,BODYMASS_Intercept)                  0.63 1.00    14647
cor(VO2_SELECTIONPoly,BODYMASS_Intercept)              0.60 1.00    12858
cor(VCO2_RQ_Intercept,BODYMASS_Intercept)              0.60 1.00    13404
cor(VCO2_RQ_SELECTIONPoly,BODYMASS_Intercept)          0.63 1.00    11628
cor(VO2_Intercept,BODYMASS_SELECTIONPoly)              0.60 1.00    20255
cor(VO2_SELECTIONPoly,BODYMASS_SELECTIONPoly)          0.61 1.00    15756
cor(VCO2_RQ_Intercept,BODYMASS_SELECTIONPoly)          0.62 1.00    15543
cor(VCO2_RQ_SELECTIONPoly,BODYMASS_SELECTIONPoly)      0.63 1.00    12527
cor(BODYMASS_Intercept,BODYMASS_SELECTIONPoly)         0.65 1.00    13003
cor(VO2_Intercept,ACTIVITY_Intercept)                  0.65 1.00    19840
cor(VO2_SELECTIONPoly,ACTIVITY_Intercept)              0.65 1.00    15524
cor(VCO2_RQ_Intercept,ACTIVITY_Intercept)              0.63 1.00    15534
cor(VCO2_RQ_SELECTIONPoly,ACTIVITY_Intercept)          0.63 1.00    12246
cor(BODYMASS_Intercept,ACTIVITY_Intercept)             0.58 1.00    11471
cor(BODYMASS_SELECTIONPoly,ACTIVITY_Intercept)         0.60 1.00     9828
cor(VO2_Intercept,ACTIVITY_SELECTIONPoly)              0.66 1.00    18961
cor(VO2_SELECTIONPoly,ACTIVITY_SELECTIONPoly)          0.67 1.00    15304
cor(VCO2_RQ_Intercept,ACTIVITY_SELECTIONPoly)          0.64 1.00    16071
cor(VCO2_RQ_SELECTIONPoly,ACTIVITY_SELECTIONPoly)      0.63 1.00    11815
cor(BODYMASS_Intercept,ACTIVITY_SELECTIONPoly)         0.58 1.00    11813
cor(BODYMASS_SELECTIONPoly,ACTIVITY_SELECTIONPoly)     0.59 1.00     8694
cor(ACTIVITY_Intercept,ACTIVITY_SELECTIONPoly)         0.64 1.00     9306
                                                   Tail_ESS
sd(VO2_Intercept)                                      7298
sd(VO2_SELECTIONPoly)                                  7871
sd(VCO2_RQ_Intercept)                                  7044
sd(VCO2_RQ_SELECTIONPoly)                              8839
sd(BODYMASS_Intercept)                                 7352
sd(BODYMASS_SELECTIONPoly)                             8496
sd(ACTIVITY_Intercept)                                 8016
sd(ACTIVITY_SELECTIONPoly)                             6315
cor(VO2_Intercept,VO2_SELECTIONPoly)                   9696
cor(VO2_Intercept,VCO2_RQ_Intercept)                  10724
cor(VO2_SELECTIONPoly,VCO2_RQ_Intercept)              11086
cor(VO2_Intercept,VCO2_RQ_SELECTIONPoly)              10005
cor(VO2_SELECTIONPoly,VCO2_RQ_SELECTIONPoly)          10169
cor(VCO2_RQ_Intercept,VCO2_RQ_SELECTIONPoly)          10637
cor(VO2_Intercept,BODYMASS_Intercept)                 10714
cor(VO2_SELECTIONPoly,BODYMASS_Intercept)             11052
cor(VCO2_RQ_Intercept,BODYMASS_Intercept)             10991
cor(VCO2_RQ_SELECTIONPoly,BODYMASS_Intercept)         11398
cor(VO2_Intercept,BODYMASS_SELECTIONPoly)              9962
cor(VO2_SELECTIONPoly,BODYMASS_SELECTIONPoly)         10667
cor(VCO2_RQ_Intercept,BODYMASS_SELECTIONPoly)         10833
cor(VCO2_RQ_SELECTIONPoly,BODYMASS_SELECTIONPoly)     11096
cor(BODYMASS_Intercept,BODYMASS_SELECTIONPoly)        11687
cor(VO2_Intercept,ACTIVITY_Intercept)                 10349
cor(VO2_SELECTIONPoly,ACTIVITY_Intercept)             11057
cor(VCO2_RQ_Intercept,ACTIVITY_Intercept)             11037
cor(VCO2_RQ_SELECTIONPoly,ACTIVITY_Intercept)         10866
cor(BODYMASS_Intercept,ACTIVITY_Intercept)            11942
cor(BODYMASS_SELECTIONPoly,ACTIVITY_Intercept)        11517
cor(VO2_Intercept,ACTIVITY_SELECTIONPoly)             10405
cor(VO2_SELECTIONPoly,ACTIVITY_SELECTIONPoly)         10555
cor(VCO2_RQ_Intercept,ACTIVITY_SELECTIONPoly)         11436
cor(VCO2_RQ_SELECTIONPoly,ACTIVITY_SELECTIONPoly)     11866
cor(BODYMASS_Intercept,ACTIVITY_SELECTIONPoly)        12454
cor(BODYMASS_SELECTIONPoly,ACTIVITY_SELECTIONPoly)    10643
cor(ACTIVITY_Intercept,ACTIVITY_SELECTIONPoly)        11230

~SAMPLE (Number of levels: 48) 
                       Estimate Est.Error l-95% CI u-95% CI Rhat Bulk_ESS
sd(VO2_Intercept)          0.98      0.17     0.66     1.33 1.00     4002
sd(VCO2_RQ_Intercept)      0.43      0.27     0.01     0.96 1.00     1393
sd(ACTIVITY_Intercept)     0.59      0.08     0.44     0.77 1.00     5220
                       Tail_ESS
sd(VO2_Intercept)          6816
sd(VCO2_RQ_Intercept)      5816
sd(ACTIVITY_Intercept)     8622

Population-Level Effects: 
                                     Estimate Est.Error l-95% CI u-95% CI Rhat
VO2_Intercept                            8.05      0.41     7.23     8.85 1.00
BODYMASS_Intercept                       0.35      0.25    -0.15     0.85 1.00
ACTIVITY_Intercept                      -0.78      0.22    -1.21    -0.34 1.00
VO2_SELECTIONPoly                       -0.14      0.52    -1.13     0.90 1.00
VO2_SEXM                                -1.01      0.49    -1.97    -0.05 1.00
VO2_CYCLEII                             -1.10      0.34    -1.75    -0.43 1.00
VO2_CYCLEIII                            -1.73      0.33    -2.36    -1.07 1.00
VO2_BODYMASS                             0.30      0.24    -0.16     0.77 1.00
VO2_ACTIVITY                             1.01      0.17     0.68     1.33 1.00
VO2_SELECTIONPoly:SEXM                   0.65      0.56    -0.47     1.74 1.00
VO2_SELECTIONPoly:CYCLEII                0.31      0.44    -0.56     1.18 1.00
VO2_SELECTIONPoly:CYCLEIII              -0.14      0.43    -0.97     0.72 1.00
VO2_SEXM:CYCLEII                         0.15      0.44    -0.72     1.01 1.00
VO2_SEXM:CYCLEIII                       -0.51      0.44    -1.38     0.37 1.00
VO2_SELECTIONPoly:SEXM:CYCLEII          -0.16      0.56    -1.27     0.94 1.00
VO2_SELECTIONPoly:SEXM:CYCLEIII          0.51      0.56    -0.59     1.61 1.00
VCO2_RQ_Intercept                       -0.01      0.32    -0.62     0.64 1.00
VCO2_RQ_SELECTIONPoly                    0.30      0.45    -0.59     1.18 1.00
VCO2_RQ_SEXM                             0.48      0.43    -0.36     1.34 1.00
VCO2_RQ_CYCLEII                          0.39      0.38    -0.34     1.16 1.00
VCO2_RQ_CYCLEIII                        -0.11      0.38    -0.85     0.65 1.00
VCO2_RQ_BODYMASS                        -0.01      0.18    -0.37     0.35 1.00
VCO2_RQ_ACTIVITY                        -0.22      0.16    -0.53     0.08 1.00
VCO2_RQ_SELECTIONPoly:SEXM               0.13      0.50    -0.87     1.10 1.00
VCO2_RQ_SELECTIONPoly:CYCLEII            0.03      0.46    -0.88     0.92 1.00
VCO2_RQ_SELECTIONPoly:CYCLEIII           0.57      0.48    -0.37     1.52 1.00
VCO2_RQ_SEXM:CYCLEII                    -0.53      0.52    -1.55     0.50 1.00
VCO2_RQ_SEXM:CYCLEIII                   -0.03      0.57    -1.16     1.10 1.00
VCO2_RQ_SELECTIONPoly:SEXM:CYCLEII      -0.47      0.61    -1.67     0.72 1.00
VCO2_RQ_SELECTIONPoly:SEXM:CYCLEIII     -0.81      0.65    -2.08     0.47 1.00
BODYMASS_SELECTIONPoly                   0.53      0.38    -0.23     1.25 1.00
BODYMASS_SEXM                           -1.02      0.26    -1.52    -0.51 1.00
BODYMASS_SELECTIONPoly:SEXM             -0.44      0.35    -1.14     0.26 1.00
ACTIVITY_SELECTIONPoly                   1.05      0.32     0.42     1.67 1.00
ACTIVITY_SEXM                            0.46      0.28    -0.10     1.01 1.00
ACTIVITY_CYCLEII                         0.19      0.19    -0.17     0.56 1.00
ACTIVITY_CYCLEIII                        0.24      0.19    -0.13     0.61 1.00
ACTIVITY_SELECTIONPoly:SEXM             -0.06      0.38    -0.81     0.69 1.00
ACTIVITY_SELECTIONPoly:CYCLEII           0.10      0.26    -0.40     0.62 1.00
ACTIVITY_SELECTIONPoly:CYCLEIII         -0.01      0.26    -0.51     0.50 1.00
ACTIVITY_SEXM:CYCLEII                   -0.48      0.26    -0.99     0.03 1.00
ACTIVITY_SEXM:CYCLEIII                  -0.68      0.26    -1.18    -0.16 1.00
ACTIVITY_SELECTIONPoly:SEXM:CYCLEII      0.32      0.35    -0.36     1.01 1.00
ACTIVITY_SELECTIONPoly:SEXM:CYCLEIII     0.52      0.35    -0.17     1.20 1.00
                                     Bulk_ESS Tail_ESS
VO2_Intercept                           10865    10859
BODYMASS_Intercept                      13100    10547
ACTIVITY_Intercept                       6658     9540
VO2_SELECTIONPoly                       10472    10634
VO2_SEXM                                 9872    10738
VO2_CYCLEII                             12285    11112
VO2_CYCLEIII                            12683    11855
VO2_BODYMASS                             8712    10373
VO2_ACTIVITY                            13529    11699
VO2_SELECTIONPoly:SEXM                  10718    11449
VO2_SELECTIONPoly:CYCLEII               13244    11467
VO2_SELECTIONPoly:CYCLEIII              13745    11369
VO2_SEXM:CYCLEII                        12860    11611
VO2_SEXM:CYCLEIII                       13469    11323
VO2_SELECTIONPoly:SEXM:CYCLEII          13443    11260
VO2_SELECTIONPoly:SEXM:CYCLEIII         14809    11546
VCO2_RQ_Intercept                        9550     9908
VCO2_RQ_SELECTIONPoly                   10808    10216
VCO2_RQ_SEXM                            11827     9973
VCO2_RQ_CYCLEII                         13056    11478
VCO2_RQ_CYCLEIII                        15659    10534
VCO2_RQ_BODYMASS                        14791    10512
VCO2_RQ_ACTIVITY                        12520    10790
VCO2_RQ_SELECTIONPoly:SEXM              10981     9932
VCO2_RQ_SELECTIONPoly:CYCLEII           14363    11092
VCO2_RQ_SELECTIONPoly:CYCLEIII          15735    11289
VCO2_RQ_SEXM:CYCLEII                    14453    10864
VCO2_RQ_SEXM:CYCLEIII                   16138    10720
VCO2_RQ_SELECTIONPoly:SEXM:CYCLEII      14530    11474
VCO2_RQ_SELECTIONPoly:SEXM:CYCLEIII     16955    11436
BODYMASS_SELECTIONPoly                  11857     9350
BODYMASS_SEXM                           16890    11227
BODYMASS_SELECTIONPoly:SEXM             16059    11830
ACTIVITY_SELECTIONPoly                   6931     8611
ACTIVITY_SEXM                            6252     8821
ACTIVITY_CYCLEII                         9521    10787
ACTIVITY_CYCLEIII                       10125    10986
ACTIVITY_SELECTIONPoly:SEXM              6210     8484
ACTIVITY_SELECTIONPoly:CYCLEII           9959    10838
ACTIVITY_SELECTIONPoly:CYCLEIII          9679    10904
ACTIVITY_SEXM:CYCLEII                    9607    10278
ACTIVITY_SEXM:CYCLEIII                   9895    10829
ACTIVITY_SELECTIONPoly:SEXM:CYCLEII      9988    10625
ACTIVITY_SELECTIONPoly:SEXM:CYCLEIII    10007    10709

Family Specific Parameters: 
               Estimate Est.Error l-95% CI u-95% CI Rhat Bulk_ESS Tail_ESS
sigma_VO2          1.05      0.08     0.90     1.21 1.00     9932    10658
sigma_VCO2         0.55      0.05     0.47     0.65 1.00     3333     8438
sigma_BODYMASS     0.68      0.09     0.54     0.87 1.00    12230     8747
sigma_ACTIVITY     0.50      0.04     0.43     0.58 1.00    10334    11254

Samples were drawn using sampling(NUTS). For each parameter, Bulk_ESS
and Tail_ESS are effective sample size measures, and Rhat is the potential
scale reduction factor on split chains (at convergence, Rhat = 1).

Posterior estimates of the effect size

Here, we calculate the effect size of the selection treatment on each response variable (and RQ) from the posterior estimates of the SEM.

Derive posterior predictions of group means

Here, we use the model to predict the means for each response variable (and the parameter RQ) in each treatment, sex and cycle (averaged across the eight replicate selection lines).

Because the model contains body mass and activity level as mediator variables, we derived the posterior predictions in three different ways, and display the answer for all three in the following figures/tables.

Firstly, we predicted the posterior means without controlling for either of the mediator variables, by deriving our predictions for each cycle, sex, and treatment with BODYMASS and ACTIVITY set to their estimated mean values for that specific cycle/sex/treatment combination. This means that (for example) if activity level positively affects respiration, and P flies evolved higher activity levels, we would predict higher VO2 in P flies due to their larger activity levels.

Secondly, we predicted the posterior means while controlling for differences in activity level between cycles, sexes, and treatments. This was accomplished by producing the posterior predictions with ACTIVITY set to its global mean value (i.e. 0), which is equivalent to asking what the response variables would be if activity levels were the same across cycles, sexes, and treatments.

Thirdly, we predicted the posterior means while controlling for differences in body mass between sexes and treatment. This was accomplished by producing the posterior predictions with BODYMASS set to its global mean value (i.e. 0), which is equivalent to asking what the response variables would be if body mass were the same across sexes and treatments.

new <- scaled_data %>%
  select(SELECTION, SEX, CYCLE) %>%
  distinct() %>%
  mutate(body_subset = TRUE)

# Find the posterior estimates of the means for the mediator variables:
bodymass_posterior <- brms_SEM_respiration %>%
  fitted(newdata = new, re_formula = NA,
         resp = "BODYMASS", summary = FALSE) %>% 
  reshape2::melt() %>%
  rename(draw = Var1, parameter_space = Var2, `Body mass` = value) %>%
  left_join(new %>% mutate(parameter_space = 1:n()), by = "parameter_space") %>%
  select(draw, SELECTION, SEX, `Body mass`) %>% as_tibble() %>% distinct() %>%
  mutate(
    treat = ifelse(SELECTION == "Mono", "M", "P"), 
    sex = ifelse(SEX == "F", "females", "males"), 
    sextreat = paste(treat, sex),
    SEX = factor(ifelse(SEX == "F", "Females", "Males"), c("Males", "Females")),
    SELECTION = ifelse(SELECTION == "Poly", "Polyandry", "Monandry")) 

activity_posterior <- brms_SEM_respiration %>%
  fitted(newdata = new, re_formula = NA,
         resp = "ACTIVITY", summary = FALSE) %>% 
  reshape2::melt() %>%
  rename(draw = Var1, parameter_space = Var2, `Activity level` = value) %>%
  left_join(new %>% mutate(parameter_space = 1:n()), by = "parameter_space") %>%
  select(draw, SELECTION, SEX, CYCLE, `Activity level`) %>% as_tibble() %>%
  mutate(
    treat = ifelse(SELECTION == "Mono", "M", "P"), 
    sex = ifelse(SEX == "F", "females", "males"), 
    sextreat = paste(treat, sex),
    SEX = factor(ifelse(SEX == "F", "Females", "Males"), c("Males", "Females")),
    SELECTION = ifelse(SELECTION == "Poly", "Polyandry", "Monandry")) 


# Get the median posterior estimate of body weight and activity,
# for the 4 combinations of sex and monogamy/polyandry treatment (and 3 cycles, for activity)
bodymass <- brms_SEM_respiration %>%
  fitted(newdata = new, re_formula = NA,
         resp = "BODYMASS") %>% as_tibble()

activity <- brms_SEM_respiration %>%
  fitted(newdata = new, re_formula = NA,
         resp = "ACTIVITY") %>% as_tibble()

new_mediators <- new %>%
  mutate(BODYMASS = bodymass$Estimate,
         ACTIVITY = activity$Estimate)

new_complete <- bind_rows(
  new_mediators %>% mutate(mediators_blocked = "Neither"),
  new_mediators %>% mutate(mediators_blocked = "Activity", 
                 ACTIVITY = 0),
  new_mediators %>% mutate(mediators_blocked = "Body mass",
                 BODYMASS = 0)) %>%
  mutate(VO2 = NA, parameter_space = 1:n()) 

post_preds_respiration <- left_join(
  brms_SEM_respiration %>%
    fitted(newdata = new_complete, re_formula = NA,
           resp = "VO2", summary = FALSE) %>% reshape2::melt() %>% 
    rename(draw = Var1, parameter_space = Var2, VO2 = value) %>%
    left_join(new_complete %>% select(-VO2), by = "parameter_space") %>% 
    as_tibble(),
  
  brms_SEM_respiration %>%
    fitted(newdata = new_complete, re_formula = NA,
           resp = "VCO2", nlpar = "RQ", summary = FALSE) %>% reshape2::melt() %>% 
    rename(draw = Var1, parameter_space = Var2, RQ = value) %>%
    left_join(new_complete %>% select(-VO2), by = "parameter_space") %>% 
    as_tibble() %>%
    mutate(RQ = 0.7 + 0.3 * inv_logit(RQ)), 
  by = c("draw", "parameter_space", "SELECTION", "SEX", "CYCLE", 
         "body_subset", "BODYMASS", "ACTIVITY", "mediators_blocked")
) %>% select(draw, VO2, RQ, SELECTION, SEX, CYCLE, mediators_blocked)

Find posterior estimates of effect size

We then calculate the effect size of treatment by subtracting the (sex-specific) mean for the M treatment from the mean for the P treatment; thus a value of 1 would mean that the P treatment has a mean that is larger by 1 standard deviation. Thus, the y-axis in the following graphs essentially shows the posterior estimate of standardised effect size (Cohen’s d), from the model shown above.

overall_SD_VO2 <- scaled_data$VO2 %>% sd()
overall_SD_RQ <- respiration$RQ %>% sd()

posterior_effect_size <- post_preds_respiration %>%
  mutate(mediators_blocked = factor(mediators_blocked, c("Neither", "Body mass", "Activity"))) %>%
  mutate(SEX = factor(ifelse(SEX == "M", "Males", "Females"), c("Males", "Females"))) %>%
  mutate(CYCLE = paste("Cycle", CYCLE)) %>%
  group_by(draw, SEX, CYCLE, mediators_blocked) %>%
  summarise(VO2_effect = (VO2[SELECTION == "Poly"] - VO2[SELECTION == "Mono"]) / overall_SD_VO2,
            RQ_effect = (RQ[SELECTION == "Poly"] - RQ[SELECTION == "Mono"]) / overall_SD_RQ,
            .groups = "drop") 

Figure

Note that the females are larger than males, and that P females are somewhat larger than M females. P individuals of both sexes are more active than M individuals, and there are changes in activity level across cycles (in particular, M males become less active with time, while P males maintain consistent, high activity levels).

p1 <- arrangeGrob(
  
  bodymass_posterior %>%
    mutate(CYCLE = "All cycles") %>%
    ggplot(aes(y = SELECTION, x = `Body mass`, fill = sextreat)) + 
    geom_vline(xintercept = 0, linetype = 2, colour = "grey20") + 
    geom_halfeyeh(alpha = 0.7, size = 0.6) +
    facet_grid(SEX ~ CYCLE) +
    coord_cartesian(xlim = c(-1.9, 1.9)) + 
    scale_fill_manual(values = cols, name = "") + 
    theme_bw() + 
    theme(legend.position = "none",
          strip.text.y = element_text(colour = "white"),
          text = element_text(family = nice_font),
          strip.background = element_rect(fill = "white", colour = "white")) + 
    ylab(NULL),
  
  activity_posterior %>%
    mutate(CYCLE = paste("Cycle", CYCLE)) %>%
    ggplot(aes(y = SELECTION, x = `Activity level`, fill = sextreat)) + 
    geom_vline(xintercept = 0, linetype = 2, colour = "grey20") + 
    stat_halfeyeh(alpha = 0.7, size = 0.6) + 
    facet_grid(SEX ~ CYCLE) +
    scale_fill_manual(values = cols, name = "") + 
    theme_bw() + 
    coord_cartesian(xlim = c(-1.5, 1.7)) + 
    theme(legend.position = "none", 
          axis.text.y = element_blank(), 
          text = element_text(family = nice_font),
          strip.background = element_blank(),
          axis.ticks.y = element_blank()) + ylab(NULL),
  nrow = 1, widths = c(0.38, 0.62), 
  left = textGrob("Selection treatment", rot = 90, gp=gpar(fontfamily = nice_font)),
  top = textGrob("A. Mediator variables (means)", hjust = 1, gp=gpar(fontfamily = nice_font)) 
)

p2 <- arrangeGrob(
  posterior_effect_size %>% 
    ggplot(aes(y = mediators_blocked, 
               x = VO2_effect,
               fill = mediators_blocked)) + 
    geom_vline(xintercept = 0, linetype = 2) + 
    stat_halfeyeh(size = 0.5, alpha = 0.5) + 
    facet_grid(SEX ~ CYCLE) +
    xlab(expression(VO[2]~(P~-~M))) + 
    ylab(NULL)  +
    scale_fill_brewer(palette = "Set2") + 
    theme_bw() +
    coord_cartesian(xlim = c(-0.6, 1.9)) + 
    theme(legend.position = "none",
          text = element_text(family = nice_font),
          strip.background = element_blank()),
  
  posterior_effect_size %>% 
    ggplot(aes(y = mediators_blocked, 
               x = RQ_effect,
               fill = mediators_blocked)) + 
    geom_vline(xintercept = 0, linetype = 2) + 
    stat_halfeyeh(size = 0.5, alpha = 0.5) + 
    facet_grid(SEX ~ CYCLE) +
    xlab("RQ (P - M)") + 
    ylab(NULL)  +
    scale_fill_brewer(palette = "Set2") + 
    theme_bw() +
    scale_x_continuous(breaks = c(-1, 0, 1)) +
    coord_cartesian(xlim = c(-1.1, 1.5)) + 
    theme(legend.position = "none",
          text = element_text(family = nice_font),
          strip.background = element_blank()),
  ncol = 1, 
  left = textGrob("Mediator variables controlled for", rot = 90, gp=gpar(fontfamily = nice_font)),
  top = textGrob("B. Respiration (effect sizes)", hjust = 1, gp=gpar(fontfamily = nice_font)) 
)

p_filler <- ggplot() + theme_void()

grid.arrange(p1, 
             arrangeGrob(p_filler, p2, p_filler, 
                         nrow = 1, widths = c(0.1, 0.8, 0.1)), 
             heights = c(0.35, 0.65)) %>%
  ggsave("figures/respiration_figure.pdf", plot = ., height = 8, width = 5)

grid.arrange(p1, 
             arrangeGrob(p_filler, p2, p_filler, 
                         nrow = 1, widths = c(0.1, 0.8, 0.1)), 
             heights = c(0.35, 0.65)) 

Version Author Date
4b2ed70 lukeholman 2020-12-18
2791e70 lukeholman 2020-12-18

Tables

Posterior estimates of the mean for mediator variables
bodymass_posterior %>%
  mutate(CYCLE = "All cycles") %>%
  select(SELECTION, SEX, CYCLE, `Body mass`, draw) %>% 
  group_by(SELECTION, SEX, CYCLE) %>%
  summarise(x = list(posterior_summary(`Body mass`) %>% 
                        as.data.frame()), .groups = "drop") %>%
  unnest(x) %>% mutate(Response = "Body weight (mean)") %>%
  bind_rows(
activity_posterior %>%
  select(SELECTION, SEX, CYCLE, `Activity level`, draw) %>%
  group_by(SELECTION, SEX, CYCLE) %>%
  summarise(x = list(posterior_summary(`Activity level`) %>% 
                        as.data.frame()), .groups = "drop") %>%
  unnest(x) %>% mutate(Response = "Activity level (mean)")) %>%
  rename(Selection = SELECTION, Sex = SEX, Cycle = CYCLE) %>%
  select(-Response) %>%
  kable(digits = 3) %>%
  kable_styling(full_width = FALSE) %>%
  pack_rows("Body weight (mean)", 1, 4) %>%
  pack_rows("Activity level (mean)", 5, 16)
Selection Sex Cycle Estimate Est.Error Q2.5 Q97.5
Body weight (mean)
Monandry Males All cycles -0.671 0.255 -1.174 -0.155
Monandry Females All cycles 0.348 0.250 -0.145 0.846
Polyandry Males All cycles -0.589 0.318 -1.249 0.016
Polyandry Females All cycles 0.873 0.308 0.236 1.458
Activity level (mean)
Monandry Males I -0.323 0.227 -0.768 0.119
Monandry Males II -0.610 0.231 -1.061 -0.164
Monandry Males III -0.761 0.231 -1.215 -0.305
Monandry Females I -0.780 0.218 -1.210 -0.339
Monandry Females II -0.588 0.226 -1.029 -0.135
Monandry Females III -0.542 0.228 -0.986 -0.089
Polyandry Males I 0.659 0.264 0.126 1.165
Polyandry Males II 0.800 0.269 0.264 1.318
Polyandry Males III 0.728 0.270 0.190 1.243
Polyandry Females I 0.266 0.257 -0.237 0.756
Polyandry Females II 0.562 0.266 0.025 1.077
Polyandry Females III 0.492 0.266 -0.037 1.010
Posterior estimates of the effect size for VO\(_2\) and RQ
posterior_effect_size %>%
  select(mediators_blocked, SEX, CYCLE, VO2_effect, draw) %>%
  group_by(mediators_blocked, SEX, CYCLE) %>%
  summarise(x = list(posterior_summary(VO2_effect) %>% 
                        as.data.frame()),
            p = 1 - as.numeric(bayestestR::p_direction(VO2_effect)),
            ` ` = ifelse(p < 0.05, "\\*", ""),
            .groups = "drop") %>%
  unnest(x) %>% mutate(Response = "VO2 effect size") %>%
  bind_rows(
    posterior_effect_size %>%
      select(mediators_blocked, SEX, CYCLE, RQ_effect, draw) %>%
      group_by(mediators_blocked, SEX, CYCLE) %>%
       summarise(x = list(posterior_summary(RQ_effect) %>% 
                        as.data.frame()),
            p = 1 - as.numeric(bayestestR::p_direction(RQ_effect)),
            ` ` = ifelse(p < 0.05, "\\*", ""),
            .groups = "drop") %>%
      unnest(x) %>% mutate(Response = "RQ effect size")) %>%
  select(-Response) %>% rename(`Mediators blocked` = mediators_blocked, Sex = SEX, Cycle = CYCLE) %>%
  kable(digits = 3) %>%
  kable_styling(full_width = FALSE) %>%
  pack_rows("VO2 effect size", 1, 18) %>%
  pack_rows("RQ effect size", 19, 36)
Mediators blocked Sex Cycle Estimate Est.Error Q2.5 Q97.5 p
VO2 effect size
Neither Males Cycle I 0.714 0.267 0.185 1.235 0.005 *
Neither Males Cycle II 0.987 0.285 0.425 1.547 0.001 *
Neither Males Cycle III 1.132 0.284 0.569 1.688 0.000 *
Neither Females Cycle I 0.504 0.234 0.046 0.970 0.015 *
Neither Females Cycle II 0.698 0.268 0.172 1.222 0.005 *
Neither Females Cycle III 0.434 0.265 -0.080 0.959 0.050 *
Body mass Males Cycle I 0.703 0.267 0.175 1.221 0.005 *
Body mass Males Cycle II 0.976 0.285 0.413 1.534 0.001 *
Body mass Males Cycle III 1.121 0.284 0.558 1.675 0.000 *
Body mass Females Cycle I 0.430 0.237 -0.033 0.898 0.034 *
Body mass Females Cycle II 0.624 0.273 0.088 1.158 0.011 *
Body mass Females Cycle III 0.360 0.269 -0.164 0.888 0.088
Activity Males Cycle I 0.250 0.278 -0.296 0.803 0.179
Activity Males Cycle II 0.320 0.305 -0.283 0.923 0.144
Activity Males Cycle III 0.428 0.306 -0.167 1.026 0.079
Activity Females Cycle I 0.009 0.242 -0.457 0.493 0.492
Activity Females Cycle II 0.154 0.281 -0.394 0.710 0.288
Activity Females Cycle III -0.055 0.277 -0.588 0.499 0.417
RQ effect size
Neither Males Cycle I 0.117 0.278 -0.453 0.639 0.326
Neither Males Cycle II -0.189 0.346 -0.865 0.490 0.290
Neither Males Cycle III -0.077 0.390 -0.811 0.700 0.417
Neither Females Cycle I 0.034 0.249 -0.463 0.523 0.444
Neither Females Cycle II 0.037 0.287 -0.536 0.595 0.447
Neither Females Cycle III 0.373 0.310 -0.255 0.969 0.116
Body mass Males Cycle I 0.118 0.279 -0.454 0.645 0.326
Body mass Males Cycle II -0.187 0.345 -0.863 0.490 0.289
Body mass Males Cycle III -0.076 0.390 -0.810 0.699 0.418
Body mass Females Cycle I 0.038 0.252 -0.471 0.525 0.436
Body mass Females Cycle II 0.039 0.288 -0.543 0.601 0.446
Body mass Females Cycle III 0.374 0.312 -0.260 0.968 0.115
Activity Males Cycle I 0.235 0.288 -0.353 0.779 0.202
Activity Males Cycle II -0.002 0.367 -0.728 0.698 0.498
Activity Males Cycle III 0.113 0.411 -0.675 0.912 0.395
Activity Females Cycle I 0.178 0.268 -0.355 0.695 0.250
Activity Females Cycle II 0.183 0.309 -0.427 0.785 0.272
Activity Females Cycle III 0.507 0.322 -0.157 1.110 0.062

Posterior difference in treatment effect size between sexes

This section essentially examines the treatment \(\times\) sex interaction term, by calculating the difference in the effect size of the P/M treatment between sexes, for each cycle, for VO\(_2\). We find that the effect of the P/M treatment on VO\(_2\) is greater in males than females, but only in the final cycle. Statistically correcting for differences in activity between sexes and treatments partly attenuated this effect, which suggests that the greater effect of treatment on activity in males is what explains the greater effect of treatment on VO2 in males.

Figure

treatsex_interaction <- posterior_effect_size %>%
  rename(Cycle = CYCLE) %>%
  group_by(draw, Cycle, mediators_blocked) %>%
  summarise(`Difference in effect size between sexes (male - female)` = VO2_effect[1] - VO2_effect[2],
            .groups = "drop")

treatsex_interaction %>%
  ggplot(aes(x = `Difference in effect size between sexes (male - female)`, y = 1, fill = stat(x < 0))) +
  geom_vline(xintercept = 0, linetype = 2) +
  stat_halfeyeh() +
  facet_grid(mediators_blocked ~ Cycle) +
  scale_fill_brewer(palette = 'Pastel2', direction = 1, name = "") +
  theme_bw() +
  theme(legend.position = 'none',
        text = element_text(family = nice_font),
        strip.background = element_blank()) +
  ylab("Posterior density")

Version Author Date
d3f10f8 lukeholman 2020-12-18

Table

treatsex_interaction %>%
  rename(x = `Difference in effect size between sexes (male - female)`) %>%
  group_by(Cycle, mediators_blocked) %>%
  summarise(`Difference in effect size between sexes (male - female)` = median(x),
            `Lower 95% CI` = quantile(x, probs = 0.025), 
            `Upper 95% CI` = quantile(x, probs = 0.975),
            p = 1 - as.numeric(bayestestR::p_direction(x)),
            ` ` = ifelse(p < 0.05, "\\*", ""),
            .groups = "drop") %>%
  kable(digits=3) %>%
  kable_styling(full_width = FALSE)
Cycle mediators_blocked Difference in effect size between sexes (male - female) Lower 95% CI Upper 95% CI p
Cycle I Neither 0.213 -0.314 0.714 0.209
Cycle I Body mass 0.275 -0.255 0.784 0.151
Cycle I Activity 0.244 -0.284 0.745 0.177
Cycle II Neither 0.295 -0.345 0.915 0.181
Cycle II Body mass 0.360 -0.287 0.982 0.139
Cycle II Activity 0.174 -0.471 0.792 0.297
Cycle III Neither 0.699 0.058 1.324 0.017 *
Cycle III Body mass 0.764 0.110 1.392 0.010 *
Cycle III Activity 0.485 -0.165 1.106 0.070

sessionInfo()
R version 4.0.3 (2020-10-10)
Platform: x86_64-apple-darwin17.0 (64-bit)
Running under: macOS Mojave 10.14.6

Matrix products: default
BLAS:   /Library/Frameworks/R.framework/Versions/4.0/Resources/lib/libRblas.dylib
LAPACK: /Library/Frameworks/R.framework/Versions/4.0/Resources/lib/libRlapack.dylib

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

attached base packages:
[1] grid      stats     graphics  grDevices utils     datasets  methods  
[8] base     

other attached packages:
 [1] knitrhooks_0.0.4   knitr_1.30         showtext_0.9-2     showtextdb_3.0    
 [5] sysfonts_0.8.3     DT_0.17            GGally_2.1.0       future.apply_1.7.0
 [9] future_1.21.0      ggridges_0.5.3     MuMIn_1.43.17      bayestestR_0.8.0  
[13] tidybayes_2.3.1    kableExtra_1.3.1   glue_1.4.2         RColorBrewer_1.1-2
[17] brms_2.14.4        Rcpp_1.0.5         gridExtra_2.3      forcats_0.5.0     
[21] stringr_1.4.0      dplyr_1.0.2        purrr_0.3.4        readr_1.4.0       
[25] tidyr_1.1.2        tibble_3.0.4       ggplot2_3.3.3      tidyverse_1.3.0   
[29] workflowr_1.6.2   

loaded via a namespace (and not attached):
  [1] readxl_1.3.1         backports_1.2.1      plyr_1.8.6          
  [4] igraph_1.2.6         splines_4.0.3        svUnit_1.0.3        
  [7] listenv_0.8.0        crosstalk_1.1.1      rstantools_2.1.1    
 [10] inline_0.3.17        digest_0.6.27        htmltools_0.5.0     
 [13] rsconnect_0.8.16     fansi_0.4.1          magrittr_2.0.1      
 [16] globals_0.14.0       modelr_0.1.8         RcppParallel_5.0.2  
 [19] matrixStats_0.57.0   xts_0.12.1           prettyunits_1.1.1   
 [22] colorspace_2.0-0     rvest_0.3.6          ggdist_2.4.0        
 [25] haven_2.3.1          xfun_0.20            callr_3.5.1         
 [28] crayon_1.3.4         jsonlite_1.7.2       lme4_1.1-26         
 [31] zoo_1.8-8            gtable_0.3.0         webshot_0.5.2       
 [34] V8_3.4.0             distributional_0.2.1 pkgbuild_1.2.0      
 [37] rstan_2.21.2         abind_1.4-5          scales_1.1.1        
 [40] mvtnorm_1.1-1        DBI_1.1.0            miniUI_0.1.1.1      
 [43] viridisLite_0.3.0    xtable_1.8-4         stats4_4.0.3        
 [46] StanHeaders_2.21.0-7 htmlwidgets_1.5.3    httr_1.4.2          
 [49] DiagrammeR_1.0.6.1   threejs_0.3.3        arrayhelpers_1.1-0  
 [52] ellipsis_0.3.1       reshape_0.8.8        farver_2.0.3        
 [55] pkgconfig_2.0.3      loo_2.4.1            dbplyr_2.0.0        
 [58] labeling_0.4.2       tidyselect_1.1.0     rlang_0.4.10        
 [61] reshape2_1.4.4       later_1.1.0.1        visNetwork_2.0.9    
 [64] munsell_0.5.0        cellranger_1.1.0     tools_4.0.3         
 [67] cli_2.2.0            generics_0.1.0       broom_0.7.3         
 [70] evaluate_0.14        fastmap_1.0.1        yaml_2.2.1          
 [73] processx_3.4.5       fs_1.5.0             nlme_3.1-149        
 [76] whisker_0.4          mime_0.9             projpred_2.0.2      
 [79] xml2_1.3.2           compiler_4.0.3       bayesplot_1.8.0     
 [82] shinythemes_1.1.2    rstudioapi_0.13      gamm4_0.2-6         
 [85] curl_4.3             reprex_0.3.0         statmod_1.4.35      
 [88] stringi_1.5.3        highr_0.8            ps_1.5.0            
 [91] Brobdingnag_1.2-6    lattice_0.20-41      Matrix_1.2-18       
 [94] nloptr_1.2.2.2       markdown_1.1         shinyjs_2.0.0       
 [97] vctrs_0.3.6          pillar_1.4.7         lifecycle_0.2.0     
[100] bridgesampling_1.0-0 insight_0.11.1       httpuv_1.5.4        
[103] R6_2.5.0             promises_1.1.1       parallelly_1.23.0   
[106] codetools_0.2-16     boot_1.3-25          colourpicker_1.1.0  
[109] MASS_7.3-53          gtools_3.8.2         assertthat_0.2.1    
[112] rprojroot_2.0.2      withr_2.3.0          shinystan_2.5.0     
[115] mgcv_1.8-33          parallel_4.0.3       hms_0.5.3           
[118] coda_0.19-4          minqa_1.2.4          rmarkdown_2.6       
[121] git2r_0.28.0         shiny_1.5.0          lubridate_1.7.9.2   
[124] base64enc_0.1-3      dygraphs_1.1.1.6