Last updated: 2022-11-30

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Knit directory: Multimodal-Plasmacell_manuscript/

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Abstract

Antibody secreting cells are key components of humoral immunity by secreting antibodies and providing protection against pathogens. These cells can be of IgM, IgA, or IgG subclass and migrate to class-specific niches. Localization and rareness of these cells make it challenge to define subclass-specific molecular hallmarks. Here, we describe how in-vitro differentiation of peripheral B-cells results in antibody-secreting cells. Using a single-cell multi-modal sequencing approach we find subclass-specific hallmark transcriptional profiles, surface protein expression and signaling pathway activity.

In this repository

The pages contain code to process, analyze and create figures presented in the full manuscript:

Attribution

We are very thankful for the efforts made by developers of Seurat, MOFA+ and workflowr. These (well documented) R-packages enable respectively extensive multi-modal data-analysis and reproducible code documentation.

The content in this repository is available under the CC BY 4.0 license.

For proper attribution, please cite our pre-print.