Last updated: 2020-10-17
Checks: 7 0
Knit directory: EMBRAPA_2020GS/
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Note that any generated files, e.g. HTML, png, CSS, etc., are not included in this status report because it is ok for generated content to have uncommitted changes.
These are the previous versions of the repository in which changes were made to the R Markdown (analysis/index.Rmd
) and HTML (docs/index.html
) files. If you’ve configured a remote Git repository (see ?wflow_git_remote
), click on the hyperlinks in the table below to view the files as they were in that past version.
File | Version | Author | Date | Message |
---|---|---|---|---|
Rmd | 20aef71 | wolfemd | 2020-10-17 | Tweaks to bulleted lists. |
html | 45cee36 | wolfemd | 2020-10-17 | Build site. |
Rmd | f309dea | wolfemd | 2020-10-17 | Start workflowr project. |
html | fa3b6e1 | wolfemd | 2020-09-01 | Build site. |
html | d97980a | wolfemd | 2020-09-01 | Build site. |
Rmd | f3a0e16 | wolfemd | 2020-09-01 | Added a link to cassavabase FTP destination for the data. |
html | d29d605 | wolfemd | 2020-09-01 | Build site. |
Rmd | 6e27397 | wolfemd | 2020-09-01 | EMBRAPA C2 has been imputed. Publish report and share to cassavabase |
html | ed70a5a | wolfemd | 2020-08-26 | Build site. |
Rmd | 9d4dfab | wolfemd | 2020-08-26 | Code organized into functions. Ready to run imputation of C2? |
Rmd | 4cc20db | wolfemd | 2020-08-26 | Start workflowr project. |
Rmd | 245ee4f | wolfemd | 2020-08-26 | Start workflowr project. |
This repository and website documents all analyses, summary, tables and figures associated with EMBRAPA genomic prediction and related procedures (e.g. imputation).
Imputation conducted in 2019: Imputation of the L. America reference panel (GBS+DArTseqLD) and EMBRAPA GS C1 (DArTseqLD only) was done in October 2019. The codes for 2019 imputation were never published in a Git repository, though they were shared internally. The 2019 imputed VCFs will serve as imputation reference panel for 2020. Therefore, I am publishing the 2019 codes here as is for reference.
DArTseqLD (DCas20-5360) arrived on Aug. 22, 2020. Contains GS C2 for EMBRAPA.
STEPS:
FILES:
DOWNLOAD FROM CASSAVABASE FTP SERVER orDOWNLOAD (files <50Mb) FROM GitHub
Last year’s reference panel for imputation had ~64K SNP. The C1 progeny imputed by it had <9K SNP after post-imputation filters. Since imputation with Beagle5.0 is very fast, I imputed C2 (DCas20-5360) with three variants of the reference panel:
RefPanel VCF filename: chr*_ImputationReferencePanel_EMBRAPA_Phased_102619.vcf.gz
Imputed C2 filename: chr*_DCas20_5360_REFimputed.vcf.gz
Post-impute filtered C2 filename: chr*_DCas20_5360_REFimputedAndFiltered.vcf.gz
Genome-wide dosage matrix format for use in R:
Imputation Reference Panel: DosageMatrix_ImputationReferencePanel_EMBRAPA_Phased_102619.rds
DCas20_5360 with standard post-impute filter: DosageMatrix_DCas20_5360_REFimputedAndFiltered.rds
DCas20_5360 with “light” post-impute filter: DosageMatrix_DCas20_5360_REFimputeLightFiltDR2pt3.rds
RefPanel VCF filename: chr*_ImputationReferencePanel_C1progenyAdded_EMBRAPA.vcf.gz
Imputed C2 filename: chr*_DCas20_5360_REFimputedWithC1unfiltered.vcf.gz
Post-impute filtered C2 filename: chr*_DCas20_5360_REFimputedWithC1unfiltered_PostImputeFiltered.vcf.gz
Genome-wide dosage matrix format for use in R:
Imputation Reference Panel: DosageMatrix_ImputationReferencePanel_C1progenyAdded_EMBRAPA.rds
DCas20_5360 with standard post-impute filter: [WARNING: 0 SNP on Chr. 4] DosageMatrix_DCas20_5360_REFimputedWithC1unfiltered_PostImputeFiltered.rds
DCas20_5360 with “light” post-impute filter: [WARNING: 0 SNP on Chr. 4] DosageMartix_DCas20_5360_REFimputedWithC1unfiltered_LightPostImputeFiltDR2pt3.rds
NOTICE: REFimputedWithC1unfiltered
is not a good dataset. Chr. 4 is entirely filtered out, including using the “lighter” filter. This emphasizes that it is not a good idea to use poorly imputed haplotypes in the reference panel… evidenced also by the fact that the REFimputedWithC1filtered
has markers passing filters on all chromosomes.
RefPanel VCF filename: chr*_ImputationReferencePanel_C1progenyAddedFilteredSites_EMBRAPA.vcf.gz
Imputed C2 filename: chr*_DCas20_5360_REFimputedWithC1filtered.vcf.gz
Post-impute filtered C2 filename: chr*_DCas20_5360_REFimputedWithC1filtered_PostImputeFiltered.vcf.gz
Genome-wide dosage matrix format for use in R:
Imputation Reference Panel: DosageMatrix_ImputationReferencePanel_C1progenyAddedFilteredSites_EMBRAPA.rds
DCas20_5360 with standard post-impute filter: DosageMatrix_DCas20_5360_REFimputedWithC1filtered_PostImputeFiltered.rds
DCas20_5360 with “light” post-impute filter: DosageMartix_DCas20_5360_REFimputedWithC1filtered_LightPostImputeFiltDR2pt3.rds
HOW TO COMBINE DOSAGE MATRICES: Users will want to combine the genotypes in the imputation reference panel files, with the genotypes in the imputed DArT file. They can have slightly different sets of markers along the columns. Here is a basic example how to combine:
snps_refpanel<-readRDS("DosageMatrix_ImputationReferencePanel_EMBRAPA_Phased_102619.rds")
snps_dcas20_5360<-readRDS("DosageMatrix_DCas20_5360_REFimputeLightFiltDR2pt3.rds")
snps2keep<-colnames(snps_refpanel)[,colnames(snps_refpanel) %in% colnames(snps_dcas20_5360)]
snps<-bind_rows(snps_refpanel[,snps2keep],
snps_dcas20_5360[,snps2keep])
SUGGESTION: Use combination PCA, prediction, correlation of kinship matrices (off-diagonals and diagonals) to compare these datasets.
The R package workflowr was used to document this study reproducibly.
Much of the supporting data and output from the analyses documented here are too large for GitHub.
The repository will be mirrored, here: ftp://ftp.cassavabase.org/marnin_datasets/EMBRAPA_2020GS/ with all data.
NOTICE: data/
and output/
are empty on GitHub. Please see ftp://ftp.cassavabase.org/marnin_datasets/EMBRAPA_2020GS/ for access.
data/
: raw data (e.g. unimputed SNP data)output/
: outputs (e.g. imputed SNP data)analysis/
: most code and workflow documented in .Rmd filesdocs/
: compiled .html, “knitted” from .RmdSupporting functions code/
The analyses in the html / Rmd files referenced above often source R scripts in the code/
sub-folder. These are wrapper functions around the packaged core functions in predCrossVar, to do the specific analyses for this paper.
R version 4.0.2 (2020-06-22)
Platform: x86_64-apple-darwin17.0 (64-bit)
Running under: macOS Mojave 10.14.6
Matrix products: default
BLAS: /Library/Frameworks/R.framework/Versions/4.0/Resources/lib/libRblas.dylib
LAPACK: /Library/Frameworks/R.framework/Versions/4.0/Resources/lib/libRlapack.dylib
locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] workflowr_1.6.2
loaded via a namespace (and not attached):
[1] Rcpp_1.0.5 whisker_0.4 knitr_1.30 magrittr_1.5
[5] R6_2.4.1 rlang_0.4.8 stringr_1.4.0 tools_4.0.2
[9] xfun_0.18 git2r_0.27.1 htmltools_0.5.0 ellipsis_0.3.1
[13] yaml_2.2.1 digest_0.6.25 rprojroot_1.3-2 tibble_3.0.4
[17] lifecycle_0.2.0 crayon_1.3.4 later_1.1.0.1 vctrs_0.3.4
[21] promises_1.1.1 fs_1.5.0 glue_1.4.2 evaluate_0.14
[25] rmarkdown_2.4 stringi_1.5.3 compiler_4.0.2 pillar_1.4.6
[29] backports_1.1.10 httpuv_1.5.4 pkgconfig_2.0.3