Last updated: 2020-12-03

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Previous step

  1. Curate by trait-trial: Model each trait-trial separately, remove outliers, get BLUPs.

This step

Three-stage procedure:

  1. Previous step: Fit mixed-model and extract BLUPs, de-regressed BLUPs and weights on a per-trial basis with one round of outlier removal x1 (as in the previous section)
  2. This step: Fit mixed-model with de-regressed BLUPs and weights from previous step as input. Get BLUPs from multi-trial analysis.
  3. Genomic prediction with drg-BLUPs from multi-trial analysis as input.

Get multi-trial BLUPs from per-trial BLUPs (three-stage)

Set-up training datasets

This next step fits models to each trait, combining curated data (BLUPs) from each trial, which we computed in the previous step.

rm(list = ls())
library(tidyverse)
library(magrittr)
source(here::here("code", "gsFunctions.R"))
dbdata <- readRDS(here::here("output", "IITA_CuratedTrials.rds"))
traits <- c("MCMDS", "DM", "PLTHT", "BRNHT1", "BRLVLS", "HI", "logFYLD", "logTOPYLD", 
    "logRTNO", "TCHART", "LCHROMO", "ACHROMO", "BCHROMO")

Starting with the curated trial data (which correspond to per-trait, per-trial BLUPs) from the previous step.

Nest by trait. Need to restructure the data from per-trial BLUPs by regrouping by trait.

dbdata <- nestForMultiTrialAnalysis(dbdata)
dbdata %>% mutate(N_blups = map_dbl(MultiTrialTraitData, nrow)) %>% rmarkdown::paged_table()

Model multiple trials

Function fitMultiTrialModel() takes de-regressed BLUPs as response and corresponding weights on error variances are applied. Output includes BLUPs for each clone that combine data across trials and are suitable for downstream genomic prediction work.

Apply the fitMultiTrialModel() to each chunk of trials (per trait) using the purrr function map().

dbdata %<>% mutate(modelOutput = map(MultiTrialTraitData, fitMultiTrialModel))
dbdata %>% select(-MultiTrialTraitData) %>% unnest(modelOutput) %>% unnest(VarComps) %>% 
    rmarkdown::paged_table()

Output file

saveRDS(dbdata, file = here::here("output", "iita_blupsForModelTraining.rds"))

Get multi-trial BLUPs from raw data (two-stage)

Between the July 2019 and current genomic evaluation for IITA, I made “upgrades” (?) to the prediction procedure. Instead of the two-stage procedure implemented in 2019, I used a three-stage procedure in 2020 (described at the top). This was previously implemented for the NRCRI prediction done in April 2020.

I think it is essential to compare the “three-stage” approach with the “two-stage”!

Two-stage procedure:

  1. Fit mixed-model to multi-trial dataset and extract BLUPs, de-regressed BLUPs and weights. Include two rounds of outlier removal.
  2. Genomic prediction with drg-BLUPs from multi-trial analysis as input.

Work below represents Stage 1 of the Two-stage procedure.

set-up training datasets

# activate multithread OpenBLAS for fast matrix algebra
export OMP_NUM_THREADS=56
rm(list = ls())
library(tidyverse)
library(magrittr)
# source(here::here('code','gsFunctions.R'))
dbdata <- readRDS(here::here("output", "IITA_ExptDesignsDetected.rds"))
traits <- c("MCMDS", "DM", "PLTHT", "BRNHT1", "BRLVLS", "HI", "logFYLD", "logTOPYLD", 
    "logRTNO", "TCHART", "LCHROMO", "ACHROMO", "BCHROMO")

Nest by trait. Need to restructure the data from per-trial by regrouping by trait.

dbdata %<>% dplyr::select(-MaxNOHAV) %>% unnest(TrialData) %>% dplyr::select(programName, 
    locationName, studyYear, TrialType, studyName, CompleteBlocks, IncompleteBlocks, 
    yearInLoc, trialInLocYr, repInTrial, blockInRep, observationUnitDbId, germplasmName, 
    FullSampleName, GID, all_of(traits), PropNOHAV) %>% mutate(IncompleteBlocks = ifelse(IncompleteBlocks == 
    TRUE, "Yes", "No"), CompleteBlocks = ifelse(CompleteBlocks == TRUE, "Yes", "No")) %>% 
    pivot_longer(cols = all_of(traits), names_to = "Trait", values_to = "Value") %>% 
    filter(!is.na(Value), !is.na(GID)) %>% nest(MultiTrialTraitData = c(-Trait))

To fit the mixed-model I used last year, I am again resorting to asreml. I fit random effects for rep and block only where complete and incomplete blocks, respectively are indicated in the trial design variables. sommer should be able to fit the same model via the at() function, but I am having trouble with it and sommer is much slower even without a dense covariance (i.e. a kinship), compared to lme4::lmer() or asreml().

dbdata %<>% mutate(fixedFormula = ifelse(Trait %in% c("logFYLD", "logRTNO", "logTOPYLD"), 
    "Value ~ yearInLoc", "Value ~ yearInLoc + PropNOHAV"), randFormula = paste0("~idv(GID) + idv(trialInLocYr) + at(CompleteBlocks,'Yes'):repInTrial ", 
    "+ at(IncompleteBlocks,'Yes'):blockInRep"))
dbdata %>% mutate(Nobs = map_dbl(MultiTrialTraitData, nrow)) %>% select(Trait, Nobs, 
    fixedFormula, randFormula) %>% rmarkdown::paged_table()
# randFormula<-paste0('~vs(GID) + vs(trialInLocYr) +
# vs(at(CompleteBlocks,'Yes'),repInTrial) +
# vs(at(IncompleteBlocks,'Yes'),blockInRep)') library(sommer) fit <- mmer(fixed =
# Value ~ 1 + yearInLoc, random = as.formula(randFormula), data=trainingdata,
# getPEV=TRUE)

Function to run asreml

Includes rounds of outlier removal and re-fitting.

fitASfunc<-function(fixedFormula,randFormula,MultiTrialTraitData,...){
  # test arguments for function
  # ----------------------
  # MultiTrialTraitData<-dbdata$MultiTrialTraitData[[7]]
  # #Trait<-dbdata$Trait[[3]]
  # fixedFormula<-dbdata$fixedFormula[[7]]
  # randFormula<-dbdata$randFormula[[7]]
  # test<-fitASfunc(fixedFormula,randFormula,MultiTrialTraitData)
  # ----------------------
  require(asreml); 
  fixedFormula<-as.formula(fixedFormula)
  randFormula<-as.formula(randFormula)
  # fit asreml 
  out<-asreml(fixed = fixedFormula,
              random = randFormula,
              data = MultiTrialTraitData, 
              maxiter = 40, workspace=800e6, na.method.X = "omit")
  #### extract residuals - Round 1
  
  outliers1<-which(abs(scale(out$residuals))>3.3)
  
  if(length(outliers1)>0){
    
    x<-MultiTrialTraitData[-outliers1,]
    # re-fit
    out<-asreml(fixed = fixedFormula,
                random = randFormula,
                data = x, 
                maxiter = 40, workspace=800e6, na.method.X = "omit")
    #### extract residuals - Round 2
    outliers2<-which(abs(scale(out$residuals))>3.3)
    if(length(outliers2)>0){
      #### remove outliers
      x<-x[-outliers2,]
      # final re-fit
      out<-asreml(fixed = fixedFormula,
                  random = randFormula,
                  data = x, maxiter = 40,workspace=800e6, na.method.X = "omit")
    }
  }
  if(length(outliers1)==0){ outliers1<-NULL }
  if(length(outliers2)==0){ outliers2<-NULL }
  
  ll<-summary(out,all=T)$loglik
  varcomp<-summary(out,all=T)$varcomp
  Vg<-varcomp["GID!GID.var","component"]
  Ve<-varcomp["R!variance","component"]
  H2=Vg/(Vg+Ve)
  blups<-summary(out,all=T)$coef.random %>%
    as.data.frame %>%
    rownames_to_column(var = "GID") %>%
    dplyr::select(GID,solution,`std error`) %>%
    filter(grepl("GID",GID)) %>%
    rename(BLUP=solution) %>%
    mutate(GID=gsub("GID_","",GID),
           PEV=`std error`^2, # asreml specific
           REL=1-(PEV/Vg), # Reliability
           drgBLUP=BLUP/REL, # deregressed BLUP
           WT=(1-H2)/((0.1 + (1-REL)/REL)*H2)) # weight for use in Stage 2
  out<-tibble(loglik=ll,Vg,Ve,H2,
              blups=list(blups),
              varcomp=list(varcomp),
              outliers1=list(outliers1),
              outliers2=list(outliers2))
  return(out) }

Run asreml

library(furrr)
options(mc.cores = 13)
plan(multiprocess)
library(asreml)
dbdata %<>% mutate(fitAS = future_pmap(., fitASfunc))
dbdata %<>% select(-fixedFormula, -randFormula, -MultiTrialTraitData) %>% unnest(fitAS)

Output file

saveRDS(dbdata, file = here::here("output", "iita_blupsForModelTraining_twostage_asreml.rds"))

Next step

  1. Check prediction accuracy: Evaluate prediction accuracy with cross-validation.

sessionInfo()
R version 4.0.2 (2020-06-22)
Platform: x86_64-apple-darwin17.0 (64-bit)
Running under: macOS Catalina 10.15.7

Matrix products: default
BLAS:   /Library/Frameworks/R.framework/Versions/4.0/Resources/lib/libRblas.dylib
LAPACK: /Library/Frameworks/R.framework/Versions/4.0/Resources/lib/libRlapack.dylib

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
 [1] lme4_1.1-25     Matrix_1.2-18   magrittr_2.0.1  forcats_0.5.0  
 [5] stringr_1.4.0   dplyr_1.0.2     purrr_0.3.4     readr_1.4.0    
 [9] tidyr_1.1.2     tibble_3.0.4    ggplot2_3.3.2   tidyverse_1.3.0
[13] workflowr_1.6.2

loaded via a namespace (and not attached):
 [1] Rcpp_1.0.5        lubridate_1.7.9.2 here_1.0.0        lattice_0.20-41  
 [5] ps_1.4.0          assertthat_0.2.1  rprojroot_2.0.2   digest_0.6.27    
 [9] R6_2.5.0          cellranger_1.1.0  backports_1.2.0   reprex_0.3.0     
[13] evaluate_0.14     httr_1.4.2        pillar_1.4.7      rlang_0.4.9      
[17] readxl_1.3.1      minqa_1.2.4       rstudioapi_0.13   nloptr_1.2.2.2   
[21] whisker_0.4       rmarkdown_2.5     splines_4.0.2     statmod_1.4.35   
[25] munsell_0.5.0     broom_0.7.2       compiler_4.0.2    httpuv_1.5.4     
[29] modelr_0.1.8      xfun_0.19         pkgconfig_2.0.3   htmltools_0.5.0  
[33] tidyselect_1.1.0  fansi_0.4.1       crayon_1.3.4      dbplyr_2.0.0     
[37] withr_2.3.0       later_1.1.0.1     MASS_7.3-53       grid_4.0.2       
[41] nlme_3.1-150      jsonlite_1.7.1    gtable_0.3.0      lifecycle_0.2.0  
[45] DBI_1.1.0         git2r_0.27.1      formatR_1.7       scales_1.1.1     
[49] cli_2.2.0         stringi_1.5.3     fs_1.5.0          promises_1.1.1   
[53] xml2_1.3.2        ellipsis_0.3.1    generics_0.1.0    vctrs_0.3.5      
[57] boot_1.3-25       tools_4.0.2       glue_1.4.2        hms_0.5.3        
[61] yaml_2.2.1        colorspace_2.0-0  rvest_0.3.6       knitr_1.30       
[65] haven_2.3.1