Last updated: 2020-03-21

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Knit directory: misc/

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Data Collection

RNA-Seq data collection

Pre-process pbmc data

Methods

susie summary

Deconvolution

limma

MAST

mouthwash

ruv4

binomial thinning ultimate: understand binomial thinning

select rr in rf using oob

single-cell

single cell DE: first try of mouthwash, cate, sva methods on single cell data GSE45719.

single cell DE PBMC: 709 CDT cells from PBMC.

Check MOUTHWASH: compare setting \(\alpha=0\) or \(\alpha=1\) in ash. Setting \(\alpha=1\) outperforms. Why? A look into binomial thinning. How about in read data analysis(instead of using thinned data)? Does setting \(\alpha=1\) give higher likelihood?

Calibration of ruv methods: whether methods acheive claimed fdr level – yes, they all perform comparably

single cell cytokine, two controls: compare two control groups.

single cell cytokine, two controls: filter out genes with < 10 non-zero elements

cisTopic scATAC-Seq