Last updated: 2020-01-20

Checks: 1 1

Knit directory: 20170327_Psen2S4Ter_RNASeq/

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File Version Author Date Message
html f154205 Steve Ped 2020-01-20 Got rid of pointless warning
Rmd bc12101 Steve Ped 2020-01-20 Added bash pipeline
html bc12101 Steve Ped 2020-01-20 Added bash pipeline
html f65bf66 Steve Ped 2020-01-20 Removed License & updated text for PCA
Rmd 39d0381 Steve Ped 2020-01-20 Finished QC
html 39d0381 Steve Ped 2020-01-20 Finished QC
Rmd acb4f8a Steve Ped 2020-01-19 Start workflowr project.

Analysis of RNASeq data from the 3way comparison of WT zebrafish samples with Heterozygous mutants for psen2S4Ter and Homozygous mutants for psen2S4Ter. This mutant is expected to be a premature termination of the psen2 gene, but instead an alternate downstream start site resulted in a near full-length transcript with significant similarity to a conventional Early Onset Familial Alzhemier’s Disease mutation.

RNAseq was performed on total RNA with \(n=4\) samples in each group. Sequencing was performed by the sequencing facility at the Centre for Cancer Biology in Adelaide, using a NextSeq. Reads were provided in as paired-end, 150bp reads.

  1. Bash Pipeline
  2. Quality Assesment