Last updated: 2020-05-31

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Knit directory: Comparative_APA/analysis/

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    Untracked:  output/fig2.pdf
    Untracked:  output/fig3.pdf
    Untracked:  output/fig4.pdf
    Untracked:  output/piecharts
    Untracked:  output/piecharts.pdf
    Untracked:  output/simpson.pdf
    Untracked:  output/whichSiteplot.pdf
    Untracked:  projectNotes.Rmd
    Untracked:  proteinModelSet.Rmd

Unstaged changes:
    Modified:   analysis/DeandNumPAS.Rmd
    Modified:   analysis/DirSelectionKhan.Rmd
    Modified:   analysis/ExploredAPA.Rmd
    Modified:   analysis/MMExpreiment.Rmd
    Modified:   analysis/OppositeMap.Rmd
    Modified:   analysis/PTM_analysis.Rmd
    Modified:   analysis/TotalDomStructure.Rmd
    Modified:   analysis/TotalVNuclearBothSpecies.Rmd
    Modified:   analysis/annotationInfo.Rmd
    Modified:   analysis/changeMisprimcut.Rmd
    Modified:   analysis/comp2apaQTLPAS.Rmd
    Modified:   analysis/correlationPhenos.Rmd
    Modified:   analysis/dInforContent.Rmd
    Modified:   analysis/diffExpression.Rmd
    Modified:   analysis/establishCutoffs.Rmd
    Modified:   analysis/incorporateQTLsAncestral.Rmd
    Modified:   analysis/investigatePantro5.Rmd
    Modified:   analysis/mRNADecay.Rmd
    Modified:   analysis/miRNAanalysis.Rmd
    Modified:   analysis/multiMap.Rmd
    Modified:   analysis/phastCon.Rmd
    Modified:   analysis/pol2.Rmd
    Modified:   analysis/speciesSpecific.Rmd

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I am interesting in understanding differential APA between human and chimpanzees. I used 3’ Seq to identify PAS for total and nuclear frations of LCL in both species.

Preparation and QC:

Choosing cuttoffs for usage and expression level

Catalog PAS:

Differential Isoform Analysis:

Quantify Phenotypes with RNA:

Intersect with outside molecular phenotypes:

Selection exploration

Explore effect size relationships

Domiance and DE (test for mechanisms)

Verify effect sizes in total

Information content

Main figures

Evaluate Reasons for inconsistant dominance structure (not rerun after annotation change 4/20)

List of everything we tried for this section:

Annotation differences

Visualization

Extra QC and Analysis