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Website to share results of fucci-seq project.

Overview

Data description

Microscopy image analysis

Processing images - from images to intensities
Image inspection
- Inspect images with multiple nuclei
- Inspect images with no nucleus
Image intensities
Shape metrics
- Nucleus shape metrics versus intensities
Normalize intensities by batch
transgene count in sequencing data
- Investigate zero-count transgenes
Fitting cellcycleR
- Parametric model across plates and individuals
- Model convergence assessment
Cluster cells based on FUCCI
“Gold standard (?)” samples

Qualty Control

Sample QC and filtering
Gene QC and filtering
- gene filtering
- PCA with technical fators

One-time investigations

Why some gene symbols (genes) correspond to multiple Ensembl IDs?

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