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1. Behavioral assay data

In the folder data/behavioral_data, the excel sheet /Raw_data/All species_50specimens_update10Minutes contains the measured from behavioral assay for 50 individuals per species per density condition. As we do not have the exact behavioral data for each specimen we sequenced, we estimated the phenotype by using the median value of each group for the following measures:

  • Moved distance (MovedDistance) in cm.

  • Stimulus zone time (StimulusZoneTime) in seconds.

  • Non-stimulus zone time (NonStimulusZoneTime) in seconds.

  • Time on stimulus wall (TimeStimulusWall) in seconds.

We created an input file with data traits with a single line being the sample name (e.g., SAMER_G_Crd_SRR11815246) and the tissue used (Head = 0 and Thorax = 1) and rearing condition (Solitary = 0 and Crowded = 1).

One note for S. gregaria, Audelia generated over 500 trials in total and so to make species comparable, we will randomly subsample 50 trials of the crowded and solitary, each. We used the following python code to select the final list.

# To select 50 samples among the CROWDED trials
import random

# Original list of samples
samples = [
    f"Trial {i}" for i in [
        21, 24, 25, 27, 28, 29, 30, 31, 32, 33, 42, 43, 44, 45, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58,
        62, 63, 65, 67, 68, 69, 70, 71, 72, 73, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89,
        98, 99, 106, 107, 108, 109, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127,
        128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147,
        148, 149, 150, 151, 152, 153, 154, 199, 200, 201, 202, 203, 205, 206, 207, 208, 209, 210, 211, 212,
        213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232,
        233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252,
        253, 254, 255, 256, 257, 258, 297, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318,
        319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338,
        343, 344, 345, 346, 347, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 431, 432, 433, 434, 435,
        436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455,
        456, 457, 458, 459, 460, 461, 462, 463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475,
        476, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 509, 510, 511, 512, 513, 514, 515, 516, 517,
        518, 519, 520, 521, 522, 523, 524, 525, 526, 527
    ]
]

# Randomly select 50 samples
random_samples = random.sample(samples, 50)
random_samples.sort()  # Optional: sort if preferred for easier readability

import pandas as pd
import ace_tools as tools

df_random_samples = pd.DataFrame(random_samples, columns=["Randomized_Samples"])
tools.display_dataframe_to_user(name="Randomized 50 Samples", dataframe=df_random_samples)

# To select 50 samples among the ISOLATED trials
# New list of trials to sample from
new_samples = [
    f"Trial {i}" for i in [
        14, 15, 16, 17, 34, 35, 36, 37, 38, 39, 41, 46, 47, 59, 60, 61, 74, 91, 92, 93, 94, 95, 96, 97,
        101, 102, 103, 104, 105, 110, 111, 112, 113, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164,
        165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183,
        184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 204, 259, 260, 261,
        262, 263, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281,
        282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 298, 299, 300, 301, 302,
        303, 304, 339, 340, 341, 342, 348, 349, 350, 351, 352, 353, 354, 356, 358, 359, 360, 361, 362,
        363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381,
        382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400,
        401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 413, 414, 415, 416, 417, 418, 419, 488,
        489, 490, 491, 492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504, 505, 506, 507,
        528, 529, 530, 531, 532, 533, 534, 535, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546,
        547, 548, 549, 550, 551
    ]
]

# Randomly select 50 samples
random_new_samples = random.sample(new_samples, 50)
random_new_samples.sort()  # Sort for readability

df_random_new_samples = pd.DataFrame(random_new_samples, columns=["Randomized_Samples"])
tools.display_dataframe_to_user(name="Randomized 50 Samples (Second List)", dataframe=df_random_new_samples)

Load the libraries

We start by loading all the required R packages.

#(install first from CRAN or Bioconductor)
library("knitr")
library("rmdformats")
library("tidyverse")
library("data.table")
library("DT")  # for making interactive search table
library("plotly") # for interactive plots
library("ggthemes") # for theme_calc
library("reshape2")
library("RColorBrewer")
library("ggpubr")
library("ggplot2")
library("ggrepel")
library("kableExtra")
library("kableExtra")
library("WGCNA")
library("ggridges")
library("dplyr")
library("cowplot")
#library("multiWGCNA")

# Path for all species
workDir <- "/Users/maevatecher/Documents/GitHub/locust-comparative-genomics/data"

Plot the behavioral data

# Define the working directory and load the data
DATA_file <- file.path(workDir, "behavioral_data/50specimens_allspecies.csv")
DATA <- read.csv(DATA_file, header = TRUE, stringsAsFactors = FALSE)
#summary(DATA)

# Adjust the 'density' column for plotting
DATA %>% dplyr::select(species, density) %>% 
  mutate(density = recode(density, Crowded = "Crowded", Isolated = "Isolated")) %>% 
  table
                    density
species              Crowded Isolated
  S.americana             50       50
  S.cancellata            50       50
  S.gregaria              50       50
  S.nitens                50       50
  S.piceifrons            50       50
  S.serialis cubense      50       50
# Define the names and limits for each variable
NAMES <- c("Distance moved (cm)", "Movement (s)", "Stimulus zone time (s)", "Non-stimulus zone time (s)", 
           "Neutral zone time (s)", "Climbing time (s)", "Rotation frequency (s)", "Stimulus wall time (s)", 
           "Distance to stimulus wall (cm)", "Velocity (cm/s)")

LIMS <- list(c(0, 1000), c(0, 750), c(0, 750), c(0, 750), c(0, 750), c(0, 750), c(0, 300), c(0, 750), c(0, 50), c(0, 8))

# Create the plots using ggplot and ggridges
PLT <- lapply(4:13, function(j) {
  DATA %>% 
    ggplot(aes(x = DATA[, j], y = species, fill = density, point_shape = 21, point_fill = density)) +
    geom_density_ridges(scale = 0.5, jittered_points = TRUE, position = "raincloud", alpha = 0.5, 
                        point_size = 2, point_alpha = 1) +
    scale_x_continuous(limits = LIMS[[j - 3]]) +
    coord_flip() +
    scale_fill_manual(values = c("#D95F02", "#1B9E77")) + 
    scale_discrete_manual(aesthetics = "point_fill", values = c("#D95F02", "#1B9E77")) +
    theme(axis.text.x = element_text(face = "italic", angle = 45, hjust = 1), 
          axis.text = element_text(color = "black", size = 12), 
          axis.title = element_text(face = "bold", size = 13), 
          legend.position = "none", 
          panel.background = element_rect(fill = NA), 
          panel.border = element_rect(color = "black", fill = NA, size = 1)) +
    labs(x = NAMES[j - 3], y = "Species") +
    scale_y_discrete(limits = c("S.gregaria", "S.cancellata", "S.piceifrons", "S.americana", "S.serialis cubense", "S.nitens"),
                     labels = c("gregaria", "cancellata", "piceifrons", "americana", "cubense", "nitens"))
})

# Create the legend
LEG <- get_legend(DATA %>% 
                    ggplot(aes(x = DATA[, 4], y = species, fill = density)) +
                    geom_density_ridges(scale = 0.5) +
                    scale_fill_manual(name = "Rearing Density", values = c("#D95F02", "#1B9E77"), labels = c("Crowded", "Isolated")) +
                    theme(legend.position = "bottom"))

# Combine the plots with the legend
plot_grid(plot_grid(plotlist = PLT, nrow = 2), LEG, rel_heights = c(1, 0.1), nrow = 2)

Version Author Date
a2d2955 Maeva TECHER 2025-07-01
###################### For publication
# List of variable names (not column numbers anymore)
selected_vars <- c(
  "Distance.moved..cm." = "Distance moved (cm)",
  "Time.in.stimulus.zone..s." = "Stimulus zone time (s)",
  "Time.in.isolated.zone..s." = "Non-stimulus zone time (s)",
  "Time.on.stimulus.wall..s." = "Stimulus wall time (s)"
)

# Matching column names to limits
lims_custom <- list(
  "Distance moved (cm)" = c(0, 1000),
  "Stimulus zone time (s)" = c(0, 750),
  "Non-stimulus zone time (s)" = c(0, 750),
  "Stimulus wall time (s)" = c(0, 750)
)

selected_vars <- c(
  "Time.on.stimulus.wall..s." = "Stimulus wall time (s)"
)

lims_custom <- list(
  "Stimulus wall time (s)" = c(0, 750)
)
# Create the plots
# Plot function without point_shape and point_fill (just use fill)
plot_behavior <- function(varname, varlabel, lims) {
  ggplot(DATA, aes(x = .data[[varname]], y = species, fill = density)) +
    geom_density_ridges(
      scale = 0.5,
      jittered_points = TRUE,
      position = "raincloud",
      point_shape = 21,
      point_size = 2,
      alpha = 0.5,
      point_alpha = 1,
      aes(point_fill = density)  # explicitly map this
    ) +
    scale_x_continuous(limits = lims) +
#    coord_flip() +
    scale_fill_manual(values = c("Crowded" = "#D95F02", "Isolated" = "#1B9E77")) +
    scale_discrete_manual(aesthetics = "point_fill", values = c("Crowded" = "#D95F02", "Isolated" = "#1B9E77")) +
    theme(
      text = element_text(family = "Helvetica"),
      axis.text.y = element_text(face = "italic", angle = 0, hjust = 1, size = 16),
      axis.text = element_text(color = "black", size = 12),
      axis.title = element_text(face = "bold", size = 20),
      legend.position = "none",
      panel.background = element_rect(fill = NA),
#      panel.border = element_rect(color = "black", fill = NA, size = 1)
      panel.border = element_blank()
    ) +
    labs(x = varlabel, y = "") +
    scale_y_discrete(
#      limits = c("S.gregaria", "S.cancellata", "S.piceifrons", "S.americana", "S.serialis cubense", "S.nitens"),
#      labels = c("gregaria", "cancellata", "piceifrons", "americana", "cubense", "nitens")
      limits = c("S.nitens", "S.serialis cubense", "S.americana", "S.piceifrons", "S.cancellata", "S.gregaria"),
      labels = c("nitens", "cubense", "americana", "piceifrons", "cancellata", "gregaria")
    )
}

# Create the 4 individual plots
custom_plots <- Map(plot_behavior,
                    names(selected_vars),
                    selected_vars,
                    lims_custom)

# Create a matching dummy plot to extract the full legend
legend_plot <- ggplot(DATA, aes(x = .data[[names(selected_vars)[1]]], y = species, fill = density)) +
  geom_density_ridges(scale = 0.5, jittered_points = TRUE,
                      point_shape = 21, aes(point_fill = density)) +
  scale_fill_manual(name = "Rearing Density", values = c("Crowded" = "#D95F02", "Isolated" = "#1B9E77")) +
  scale_discrete_manual(aesthetics = "point_fill", values = c("Crowded" = "#D95F02", "Isolated" = "#1B9E77")) +
  theme_minimal() +
  theme(legend.position = "bottom")

# Extract legend
LEGEND <- cowplot::get_legend(legend_plot)

# Combine plots and legend
final_plot <- plot_grid(
  plot_grid(plotlist = custom_plots, ncol = 1),
  LEGEND,
  rel_heights = c(1, 0.12),
  ncol = 1
)

# Display final plot
print(final_plot)

Version Author Date
a2d2955 Maeva TECHER 2025-07-01

We then took the median of each density and species to assign phenotypes to our transcriptomic data. We are aware that the behavioral data are only approximation as the individual behavior was not collected but we aim to use gene-network analysis to see some association with behavior.

# Load behavioral trait data
behavior_file <- file.path(workDir, "behavioral_data/Sample_phenotypes_WGCNA.csv")
behavior_df <- read.csv(behavior_file, header = TRUE, stringsAsFactors = FALSE)

# Display the behavioral data
datatable(behavior_df, options = list(
  pageLength = 10,       # Set initial page length
  scrollX = TRUE,        # Enable horizontal scrolling
  autoWidth = TRUE,      # Adjust column width automatically
  searchHighlight = TRUE # Highlight search matches
), rownames = FALSE, escape = FALSE)

Gene Co-Expression Network Analysis

Step 1: Load Normalized Data from DESeq2

We are taking as input the vst (varianceStabilizingTransformation) from our DESeq2 results.

# Define species and tissue
species <- "gregaria"
tissue <- "Thorax"

# Load datExpr file
datExpr_file <- file.path(workDir, "WGCNA/input/Bulk_RNAseq", paste0("datExpr_", tissue, "_", species, ".csv"))
datExpr <- read.csv(datExpr_file, row.names = 1, check.names = FALSE)  # Avoid unwanted renaming

# Ensure data is formatted correctly
datExpr <- as.data.frame(datExpr)

# Display dimensions
cat("✅ Loaded datExpr for", species, "-", tissue, "with dimensions:", dim(datExpr), "\n")
✅ Loaded datExpr for gregaria - Thorax with dimensions: 10 12361 
# Display first few row and column names for verification
cat("🔍 First few row names (should be genes or samples):\n")
🔍 First few row names (should be genes or samples):
print(head(rownames(datExpr)))
[1] "SGRE-THOX-CRD-1" "SGRE-THOX-CRD-3" "SGRE-THOX-CRD-4" "SGRE-THOX-CRD-5"
[5] "SGRE-THOX-CRD-6" "SGRE-THOX-ISO-1"
cat("🔍 First few column names (should be samples or genes):\n")
🔍 First few column names (should be samples or genes):
print(head(colnames(datExpr)))
[1] "LOC126318536" "LOC126318656" "LOC126318743" "LOC126319294" "LOC126319460"
[6] "LOC126320026"
# **Check if datExpr needs transposition**
if (grepl("Sample|^S[0-9]+$", rownames(datExpr)[1])) {
    warning("⚠️ datExpr appears transposed. Transposing now...")
    datExpr <- t(datExpr)
    datExpr <- as.data.frame(datExpr)  # Ensure it's still a dataframe after transposition
}

# **Ensure genes are rows and samples are columns**
if (length(setdiff(rownames(datExpr), colnames(datExpr))) == 0) {
    stop("❌ datExpr row names match column names, indicating potential incorrect format. Check input file.")
}

# **Convert all values to numeric**
datExpr[] <- lapply(datExpr, as.numeric)  # Faster and avoids redundant reassignment

# **Final structure verification**
cat("✅ Verified datExpr structure. Dimensions:", dim(datExpr), "\n")
✅ Verified datExpr structure. Dimensions: 10 12361 
cat("🔍 First few row names (should be genes):\n")
🔍 First few row names (should be genes):
print(head(rownames(datExpr)))
[1] "SGRE-THOX-CRD-1" "SGRE-THOX-CRD-3" "SGRE-THOX-CRD-4" "SGRE-THOX-CRD-5"
[5] "SGRE-THOX-CRD-6" "SGRE-THOX-ISO-1"
cat("🔍 First few column names (should be samples):\n")
🔍 First few column names (should be samples):
print(head(colnames(datExpr)))
[1] "LOC126318536" "LOC126318656" "LOC126318743" "LOC126319294" "LOC126319460"
[6] "LOC126320026"

Step 2: Align Behavioral Data

# Load behavioral trait data
behavior_file <- file.path(workDir, "behavioral_data/Sample_phenotypes_WGCNA.csv")
behavior_df <- read.csv(behavior_file, header = TRUE, stringsAsFactors = FALSE)

# Align datExpr with behavioral data
samples <- rownames(datExpr)
traitRows <- match(samples, behavior_df$SampleName)
datTraits <- behavior_df[traitRows, , drop = FALSE]

# Check for missing matches
if (any(is.na(traitRows))) {
  warning("⚠ Some samples in datExpr do not match behavioral data. Check row names in behavior_df.")
}

# Ensure datTraits has correct row names
rownames(datTraits) <- datTraits$SampleName
datTraits <- datTraits[ , -which(names(datTraits) == "SampleName"), drop = FALSE]  # Remove SampleName column if present

# Check dimensions
cat("✅ Aligned behavioral data with dimensions:", dim(datTraits), "\n")
✅ Aligned behavioral data with dimensions: 10 8 

Step 3: Check Data Integrity

# Check for good samples and genes
gsg <- goodSamplesGenes(datExpr, verbose = 3)
 Flagging genes and samples with too many missing values...
  ..step 1
print(paste("All ok?", gsg$allOK))
[1] "All ok? TRUE"
# Remove problematic samples and genes, if any
if (!gsg$allOK) {
    datExpr <- datExpr[gsg$goodSamples, gsg$goodGenes]
    cat("🛠 Removed problematic samples and genes.\n")
}

# We need to check if there are outliers samples that we should remove
samplesTree <- hclust(d = dist(datExpr), method = "average")
plot(samplesTree, main = "Samples dendrogram", sub = "", xlab = "", cex.lab = 1, cex.axis = 1, cex.main = 1, cex = 0.5)
abline(h = 28, col = "red")

Version Author Date
a2d2955 Maeva TECHER 2025-07-01
# Ensure datTraits aligns with datExpr
samples <- rownames(datExpr)
traitRows <- match(samples, rownames(datTraits))
datTraits <- datTraits[traitRows, , drop = FALSE]

# Warn if any mismatch remains
if (any(is.na(traitRows))) {
    warning("⚠ Some samples in datExpr do not match datTraits. Verify sample names.")
}

cat("✅ Cleaned datExpr dimensions:", dim(datExpr), "\n")
✅ Cleaned datExpr dimensions: 10 12361 
cat("✅ Final aligned datTraits dimensions:", dim(datTraits), "\n")
✅ Final aligned datTraits dimensions: 10 8 

Step 4 : Choose a soft threshold

# Define power range to test
powers <- c(1:10, seq(12, 30, by = 2))

# Estimate the soft threshold
sft <- pickSoftThreshold(datExpr, powerVector = powers, verbose = 5)
pickSoftThreshold: will use block size 3619.
 pickSoftThreshold: calculating connectivity for given powers...
   ..working on genes 1 through 3619 of 12361
   ..working on genes 3620 through 7238 of 12361
   ..working on genes 7239 through 10857 of 12361
   ..working on genes 10858 through 12361 of 12361
   Power SFT.R.sq  slope truncated.R.sq mean.k. median.k. max.k.
1      1    0.262  0.822          0.373  4550.0   4490.00   6560
2      2    0.227 -0.317          0.236  2400.0   2180.00   4540
3      3    0.813 -0.690          0.790  1480.0   1210.00   3480
4      4    0.923 -0.859          0.907  1010.0    729.00   2810
5      5    0.947 -0.950          0.935   729.0    480.00   2350
6      6    0.953 -1.000          0.942   551.0    330.00   2010
7      7    0.954 -1.040          0.945   430.0    235.00   1750
8      8    0.949 -1.080          0.941   344.0    170.00   1550
9      9    0.943 -1.110          0.938   282.0    125.00   1390
10    10    0.935 -1.130          0.931   234.0     94.60   1250
11    12    0.935 -1.160          0.937   169.0     56.70   1040
12    14    0.925 -1.190          0.931   127.0     35.80    882
13    16    0.918 -1.210          0.928    98.0     23.60    761
14    18    0.915 -1.220          0.929    77.8     16.10    665
15    20    0.912 -1.240          0.930    63.0     11.40    587
16    22    0.914 -1.240          0.934    51.9      8.24    523
17    24    0.910 -1.250          0.934    43.4      6.09    469
18    26    0.911 -1.260          0.937    36.7      4.60    423
19    28    0.915 -1.260          0.942    31.3      3.54    384
20    30    0.918 -1.270          0.946    27.0      2.78    351
# Check power selection
chosen_power <- sft$powerEstimate
if (is.na(chosen_power)) {
    stop("❌ No suitable soft-threshold power found. Check your data.")
} else {
    cat("✅ Chosen soft-threshold power:", chosen_power, "\n")
}
✅ Chosen soft-threshold power: 4 
# Create output directory for plots
output_dir <- file.path(workDir, "WGCNA/output/Bulk_RNAseq", species)
dir.create(output_dir, showWarnings = FALSE, recursive = TRUE)

# Plot results
plot_file <- file.path(output_dir, paste0("SoftThreshold_", tissue, "_", species, ".pdf"))
pdf(plot_file, width = 8, height = 6)
par(mfrow = c(1, 2))

corRaw = abs(cor(datExpr))
corSoft = abs(corRaw**sft$powerEstimate)
hist(corRaw, main="Raw correlations")
hist(corSoft, main="Soft-thresholded correlations")

# Scale-free topology plot
plot(
  sft$fitIndices[, 1],
  -sign(sft$fitIndices[, 3]) * sft$fitIndices[, 2],
  type = "b", col = "blue", pch = 16,
  xlab = "Soft Threshold (Power)",
  ylab = expression("Scale-Free Topology Fit" ~ R^2),
  main = "Scale-Free Topology Fit"
)
abline(v = seq(2, max(powers), by = 2), col = "lightgray", lty = "dashed")  # Vertical gridlines
abline(h = 0.80, col = "black", lty = 2)  # Threshold at R^2 = 0.8
abline(h = 0.90, col = "red", lty = 2)  # Threshold at R^2 = 0.9

# Mean connectivity plot
plot(
  sft$fitIndices[, 1],
  sft$fitIndices[, 5],
  type = "b", col = "darkgreen", pch = 16,
  xlab = "Soft Threshold (Power)",
  ylab = "Mean Connectivity",
  main = "Mean Connectivity"
)
abline(v = seq(2, max(powers), by = 2), col = "lightgray", lty = "dashed")

dev.off()
quartz_off_screen 
                2 
# Output message
cat("✅ Soft threshold estimation plot saved to:", plot_file, "\n")
✅ Soft threshold estimation plot saved to: /Users/maevatecher/Documents/GitHub/locust-comparative-genomics/data/WGCNA/output/Bulk_RNAseq/gregaria/SoftThreshold_Thorax_gregaria.pdf 

Step 5: Network Construction and Module Detection

Control step

# Ensure datExpr is correctly formatted
if (!all(sapply(datExpr, is.numeric))) {
    stop("❌ datExpr contains non-numeric values. Check your input data.")
}

# Ensure no missing values
if (anyNA(datExpr)) {
    stop("❌ datExpr contains missing values. Please clean your data.")
}

# Check alignment between datExpr and datTraits
if (!all(rownames(datExpr) == rownames(datTraits))) {
    stop("❌ Row names of datExpr and datTraits do not match. Verify alignment.")
}

# Convert non-numeric traits to numeric
non_numeric_cols <- which(sapply(datTraits, class) %in% c("character", "factor"))
if (length(non_numeric_cols) > 0) {
    for (col in non_numeric_cols) {
        datTraits[[col]] <- as.numeric(as.factor(datTraits[[col]]))
    }
}

# Align `datTraits` with `datExpr`
samples <- rownames(datExpr)
traitRows <- match(samples, rownames(datTraits))
datTraits <- datTraits[traitRows, , drop = FALSE]

# Convert all non-numeric columns in `datTraits` to numeric
numeric_traits <- datTraits
if (length(non_numeric_cols) > 0) {
  for (col in non_numeric_cols) {
    numeric_traits[[col]] <- as.numeric(as.factor(datTraits[[col]]))
  }
}

cat("✅ Numeric traits created with dimensions:", dim(numeric_traits), "\n")
✅ Numeric traits created with dimensions: 10 8 
cat("✅ datExpr dimensions:", dim(datExpr), "\n")
✅ datExpr dimensions: 10 12361 
cat("✅ Numeric traits dimensions:", dim(numeric_traits), "\n")
✅ Numeric traits dimensions: 10 8 
if (nrow(datExpr) != nrow(numeric_traits)) {
    stop("❌ Number of samples in datExpr and numeric_traits do not match.")
}

# Find genes with zero MAD
zero_mad_genes <- rownames(datExpr)[apply(datExpr, 1, mad) == 0]

cat("⚠️ Genes with zero MAD:", length(zero_mad_genes), "\n")
⚠️ Genes with zero MAD: 0 
# Optionally, inspect some of them
if (length(zero_mad_genes) > 0) {
    print(zero_mad_genes[1:min(10, length(zero_mad_genes))])  # Show up to 10 genes
}

Constructing the network

picked_power <- 12
CutHeight <- 0.2

#similarityMat <- adjacency(datExpr, power = Power)
#head(similarityMat[, c(1:5)])
#TOM <- TOMsimilarity(similarityMat)
#colnames(TOM) <- colnames(similarityMat)
#rownames(TOM) <- rownames(similarityMat)
#head(TOM[, c(1:5)])
#dissTOM <- 1 - TOM
#head(dissTOM[, c(1:5)])

# Perform network construction and module detection
net <- blockwiseModules(
    datExpr,
    
    # == Adjacency Function ==
    power = picked_power,
    networkType = "unsigned",
    
    # == Tree and Block Options ==
    maxBlockSize = 20000,    # Adjust for large datasets
    minModuleSize = 30,      # Minimum number of genes per module
    reassignThreshold = 0,   # No reassignment of weak genes    

    # == Module merging options ==
    mergeCutHeight = CutHeight,   # Threshold for merging similar modules
    
    # == Output options ==    
    numericLabels = FALSE,   # Use color labels instead of numeric
    verbose = 5
)
 Calculating module eigengenes block-wise from all genes
   Flagging genes and samples with too many missing values...
    ..step 1
 ..Working on block 1 .
    TOM calculation: adjacency..
    ..will not use multithreading.
     Fraction of slow calculations: 0.000000
    ..connectivity..
    ..matrix multiplication (system BLAS)..
    ..normalization..
    ..done.
 ....clustering..
 ....detecting modules..
     ..done.
 ....calculating module eigengenes..
     moduleEigengenes : Working on ME for module 1
     moduleEigengenes : Working on ME for module 2
     moduleEigengenes : Working on ME for module 3
     moduleEigengenes : Working on ME for module 4
     moduleEigengenes : Working on ME for module 5
     moduleEigengenes : Working on ME for module 6
     moduleEigengenes : Working on ME for module 7
     moduleEigengenes : Working on ME for module 8
     moduleEigengenes : Working on ME for module 9
     moduleEigengenes : Working on ME for module 10
     moduleEigengenes : Working on ME for module 11
     moduleEigengenes : Working on ME for module 12
     moduleEigengenes : Working on ME for module 13
     moduleEigengenes : Working on ME for module 14
     moduleEigengenes : Working on ME for module 15
     moduleEigengenes : Working on ME for module 16
     moduleEigengenes : Working on ME for module 17
     moduleEigengenes : Working on ME for module 18
     moduleEigengenes : Working on ME for module 19
     moduleEigengenes : Working on ME for module 20
     moduleEigengenes : Working on ME for module 21
     moduleEigengenes : Working on ME for module 22
     moduleEigengenes : Working on ME for module 23
     moduleEigengenes : Working on ME for module 24
     moduleEigengenes : Working on ME for module 25
     moduleEigengenes : Working on ME for module 26
     moduleEigengenes : Working on ME for module 27
     moduleEigengenes : Working on ME for module 28
     moduleEigengenes : Working on ME for module 29
     moduleEigengenes : Working on ME for module 30
     moduleEigengenes : Working on ME for module 31
     moduleEigengenes : Working on ME for module 32
     moduleEigengenes : Working on ME for module 33
     moduleEigengenes : Working on ME for module 34
     moduleEigengenes : Working on ME for module 35
     moduleEigengenes : Working on ME for module 36
     moduleEigengenes : Working on ME for module 37
     moduleEigengenes : Working on ME for module 38
     moduleEigengenes : Working on ME for module 39
     moduleEigengenes : Working on ME for module 40
     moduleEigengenes : Working on ME for module 41
     moduleEigengenes : Working on ME for module 42
     moduleEigengenes : Working on ME for module 43
     moduleEigengenes : Working on ME for module 44
     moduleEigengenes : Working on ME for module 45
     moduleEigengenes : Working on ME for module 46
     moduleEigengenes : Working on ME for module 47
     moduleEigengenes : Working on ME for module 48
     moduleEigengenes : Working on ME for module 49
 ....checking kME in modules..
     ..removing 1 genes from module 2 because their KME is too low.
     ..removing 1 genes from module 10 because their KME is too low.
 ..merging modules that are too close..
     mergeCloseModules: Merging modules whose distance is less than 0.2
       multiSetMEs: Calculating module MEs.
         Working on set 1 ...
         moduleEigengenes: Calculating 50 module eigengenes in given set.
       multiSetMEs: Calculating module MEs.
         Working on set 1 ...
         moduleEigengenes: Calculating 33 module eigengenes in given set.
       multiSetMEs: Calculating module MEs.
         Working on set 1 ...
         moduleEigengenes: Calculating 29 module eigengenes in given set.
       Calculating new MEs...
       multiSetMEs: Calculating module MEs.
         Working on set 1 ...
         moduleEigengenes: Calculating 29 module eigengenes in given set.
# Save the network object for future reference
output_file <- file.path(workDir, "WGCNA/output/Bulk_RNAseq", species, paste0("network_", tissue, "_", species, ".rds"))
saveRDS(net, file = output_file)

cat("✅ Network construction completed and saved to:", output_file, "\n")
✅ Network construction completed and saved to: /Users/maevatecher/Documents/GitHub/locust-comparative-genomics/data/WGCNA/output/Bulk_RNAseq/gregaria/network_Thorax_gregaria.rds 

Visualizing Module Dendrogram

# Plot module dendrogram
plot_file <- file.path(workDir, "WGCNA/output/Bulk_RNAseq", species, paste0("ModuleDendrogram_", tissue, "_", species, ".pdf"))
pdf(plot_file, width = 12, height = 8)

plotDendroAndColors(
    net$dendrograms[[1]],
    net$colors[net$blockGenes[[1]]],
    "Module Colors",
    dendroLabels = FALSE,
    hang = 0.03,
    addGuide = TRUE,
    guideHang = 0.05
)
dev.off()
quartz_off_screen 
                2 
cat("✅ Module dendrogram plot saved to:", plot_file, "\n")
✅ Module dendrogram plot saved to: /Users/maevatecher/Documents/GitHub/locust-comparative-genomics/data/WGCNA/output/Bulk_RNAseq/gregaria/ModuleDendrogram_Thorax_gregaria.pdf 

Visualizing Module Sizes

# Calculate module sizes
module_sizes <- table(net$colors)

# Print module sizes
print(module_sizes)

        black          blue         brown          cyan     darkgreen 
          298           984           858           161            81 
     darkgrey    darkorange       darkred darkturquoise         green 
           79            68            98            79           488 
  greenyellow          grey        grey60     lightcyan    lightgreen 
          205          1586           117           135           108 
  lightyellow       magenta  midnightblue        orange          pink 
          101           252           140            73           295 
       purple           red     royalblue        salmon       skyblue 
          248           304            99           184            66 
          tan     turquoise         white        yellow 
          202          4391            67           594 
# Save module sizes
output_file <- file.path(workDir, "WGCNA/output/Bulk_RNAseq", species, paste0("ModuleSizes_", tissue, "_", species, ".csv"))
write.csv(module_sizes, file = output_file, row.names = TRUE)

# Bar plot for module sizes
plot_file <- file.path(workDir, "WGCNA/output/Bulk_RNAseq", species, paste0("ModuleSizes_", tissue, "_", species, ".pdf"))
pdf(plot_file, width = 10, height = 6)

barplot(
    module_sizes,
    main = "Module Sizes",
    xlab = "Modules",
    ylab = "Number of Genes",
    col = names(module_sizes),
    las = 2  # Rotate axis labels
)
dev.off()
quartz_off_screen 
                2 
cat("✅ Module sizes plot saved to:", plot_file, "\n")
✅ Module sizes plot saved to: /Users/maevatecher/Documents/GitHub/locust-comparative-genomics/data/WGCNA/output/Bulk_RNAseq/gregaria/ModuleSizes_Thorax_gregaria.pdf 

Step 6: Module-Trait Relationships

Check Data before correlation

# Ensure all traits are numeric
numeric_traits <- datTraits
non_numeric_cols <- which(sapply(datTraits, class) %in% c("character", "factor"))

if (length(non_numeric_cols) > 0) {
  for (col in non_numeric_cols) {
    numeric_traits[[col]] <- as.numeric(as.factor(datTraits[[col]]))
  }
}

# Confirm alignment
cat("✅ Converted traits to numeric. Dimensions:", dim(numeric_traits), "\n")
✅ Converted traits to numeric. Dimensions: 10 8 
cat("✅ datExpr dimensions:", dim(datExpr), "\n")
✅ datExpr dimensions: 10 12361 
if (nrow(datExpr) != nrow(numeric_traits)) {
  stop("❌ Number of samples in datExpr and numeric_traits do not match.")
}

Calculate Module Eigengenes and correlation

# Calculate module eigengenes
module_eigengenes <- moduleEigengenes(datExpr, colors = net$colors)$eigengenes
module_eigengenes <- orderMEs(module_eigengenes)

# Correlate module eigengenes with traits
module_trait_correlation <- cor(module_eigengenes, numeric_traits, use = "p")
module_trait_pvalues <- corPvalueStudent(module_trait_correlation, nrow(datExpr))

cat("✅ Module-trait correlation calculated!\n")
✅ Module-trait correlation calculated!
# Save correlation and p-values
correlation_file <- file.path(workDir, "WGCNA/output/Bulk_RNAseq", species, paste0("ModuleTraitCorrelation_", tissue, "_", species, ".csv"))
pvalues_file <- file.path(workDir, "WGCNA/output/Bulk_RNAseq", species, paste0("ModuleTraitPValues_", tissue, "_", species, ".csv"))

write.csv(module_trait_correlation, correlation_file, row.names = TRUE)
write.csv(module_trait_pvalues, pvalues_file, row.names = TRUE)

cat("✅ Module-trait correlation data saved to:", correlation_file, "\n")
✅ Module-trait correlation data saved to: /Users/maevatecher/Documents/GitHub/locust-comparative-genomics/data/WGCNA/output/Bulk_RNAseq/gregaria/ModuleTraitCorrelation_Thorax_gregaria.csv 
cat("✅ Module-trait p-values saved to:", pvalues_file, "\n")
✅ Module-trait p-values saved to: /Users/maevatecher/Documents/GitHub/locust-comparative-genomics/data/WGCNA/output/Bulk_RNAseq/gregaria/ModuleTraitPValues_Thorax_gregaria.csv 

Heatmap visualization module trait relationships

# Extract module colors without "ME"
module_colors <- gsub("^ME", "", names(module_eigengenes))

# Create labels with color and corresponding gene count
module_labels <- paste0(module_colors, " (", module_sizes[module_colors], ")")

# Heatmap of module-trait relationships
plot_file <- file.path(workDir, "WGCNA/output/Bulk_RNAseq", species, paste0("ModuleTraitRelationships_", tissue, "_", species, "_with_colors.pdf"))
pdf(plot_file, width = 12, height = 20)
labeledHeatmap(
  Matrix = module_trait_correlation,
  xLabels = colnames(numeric_traits),
  yLabels = names(module_eigengenes),
  colorLabels = FALSE,
  colors = blueWhiteRed(50),
  textMatrix = paste0(
    signif(module_trait_correlation, 2), "\n(",
    signif(module_trait_pvalues, 1), ")"
  ),
  cex.text = 0.8,
  zlim = c(-1, 1),
  main = paste("Module-Trait Relationships -", tissue, species)
)
dev.off()
quartz_off_screen 
                2 
cat("✅ Module-trait relationships heatmap with real colors saved to:", plot_file, "\n")
✅ Module-trait relationships heatmap with real colors saved to: /Users/maevatecher/Documents/GitHub/locust-comparative-genomics/data/WGCNA/output/Bulk_RNAseq/gregaria/ModuleTraitRelationships_Thorax_gregaria_with_colors.pdf 
# Heatmap of module-trait relationships
plot_file <- file.path(workDir, "WGCNA/output/Bulk_RNAseq", species, paste0("ModuleTraitRelationships_", tissue, "_", species, "_with_colors_name.pdf"))
pdf(plot_file, width = 12, height = 20)
labeledHeatmap(
  Matrix = module_trait_correlation,
  xLabels = colnames(numeric_traits),
  yLabels = module_labels,  # Add color names to labels
  colorLabels = FALSE,
  colors = blueWhiteRed(50),
  textMatrix = paste0(
    signif(module_trait_correlation, 2), "\n(",
    signif(module_trait_pvalues, 1), ")"
  ),
  cex.text = 0.8,
  zlim = c(-1, 1),
  main = paste("Module-Trait Relationships -", tissue, species)
)
dev.off()
quartz_off_screen 
                2 
cat("Module-trait relationships plot with colors saved to:", plot_file, "\n")
Module-trait relationships plot with colors saved to: /Users/maevatecher/Documents/GitHub/locust-comparative-genomics/data/WGCNA/output/Bulk_RNAseq/gregaria/ModuleTraitRelationships_Thorax_gregaria_with_colors_name.pdf 

Module–Module Co‐expression Network

library(WGCNA)
library(visNetwork)
library(scales)

# 1) Compute module eigengenes (reuse from Step 6)
MEs <- moduleEigengenes(datExpr, colors = net$colors)$eigengenes
MEs <- orderMEs(MEs)

# 2) Correlate eigengenes and threshold
modCor <- cor(MEs, use = "pairwise.complete.obs")
edge_thr <- 0.3
modCor[ abs(modCor) < edge_thr ] <- 0

# 3) Build edge list (only one direction)
modules  <- colnames(modCor)
idx      <- which(modCor != 0, arr.ind = TRUE)
edges_df <- data.frame(
  from   = modules[idx[,1]],
  to     = modules[idx[,2]],
  weight = abs(modCor[idx]),
  stringsAsFactors = FALSE
)
edges_df <- edges_df[idx[,1] < idx[,2], ]

# 4) Prepare nodes
modSizes <- table(net$colors)
nodes_df <- data.frame(
  id    = modules,
  label = gsub("^ME", "", modules),  # drop the "ME" prefix for the label
  title = paste0(gsub("^ME","", modules),
                 "<br>Genes: ", modSizes[gsub("^ME","",modules)]),
  value = as.numeric(modSizes[gsub("^ME","",modules)]),
  stringsAsFactors = FALSE
)

# Assign each node its WGCNA color by stripping "ME" from the name
nodes_df$color.background <- gsub("^ME", "", modules)
nodes_df$color.border     <- "black"
nodes_df$color.highlight.background <- gsub("^ME", "", modules)
nodes_df$color.highlight.border     <- "black"

# 5) Interactive visNetwork
visNetwork(nodes_df, edges_df, height = "600px", width = "100%") %>%
  visIgraphLayout(layout = "layout_with_fr", randomSeed = 42) %>%
  visNodes(
    shape = "dot",
    shadow = TRUE
    # We no longer need to set color here,
    # visNetwork will pick up color.background & color.border from nodes_df
  ) %>%
  visEdges(
    smooth = FALSE,
    color = list(color = "grey80")
  ) %>%
  visOptions(
    highlightNearest  = TRUE,
    nodesIdSelection  = TRUE
  ) %>%
  visLegend(useGroups = FALSE)  # legend not needed since node colors are explicit

Step 7: Identifying Hub Genes in Modules

Control check module membership calculation

# Calculate module membership (MM)
module_membership <- as.data.frame(cor(datExpr, module_eigengenes, use = "p"))

# Ensure proper column names without prefixes
colnames(module_membership) <- gsub("^ME", "", colnames(module_membership))

# Add the module colors to the data
module_membership$ModuleColor <- net$colors

# Calculate p-values for module membership
module_membership_pvalues <- as.data.frame(
  corPvalueStudent(as.matrix(module_membership[, -ncol(module_membership)]), nrow(datExpr))
)

# Ensure proper column names for p-values
colnames(module_membership_pvalues) <- paste0("p.", colnames(module_membership)[-ncol(module_membership)])

# Save module membership and p-values
output_file <- file.path(workDir, "WGCNA/output/Bulk_RNAseq", species, paste0("ModuleMembership_", tissue, "_", species, ".csv"))
write.csv(cbind(module_membership, module_membership_pvalues), file = output_file, row.names = TRUE)

cat("✅ Module membership data saved to:", output_file, "\n")
✅ Module membership data saved to: /Users/maevatecher/Documents/GitHub/locust-comparative-genomics/data/WGCNA/output/Bulk_RNAseq/gregaria/ModuleMembership_Thorax_gregaria.csv 

Identify Hub genes

# Define the target module color
target_module_color <- "green"  # Change to desired module color

# Ensure the correct column exists
if (!target_module_color %in% colnames(module_membership)) {
    stop(paste("❌ Column", target_module_color, "not found in module_membership. Check column names."))
}

# Filter for hub genes (Module Membership > 0.8)
hub_genes <- module_membership[
    module_membership$ModuleColor == target_module_color & 
    abs(module_membership[[target_module_color]]) > 0.8, 
]

# Save hub genes
hub_genes_file <- file.path(workDir, "WGCNA/output/Bulk_RNAseq", species, paste0("HubGenes_", target_module_color, "_", tissue, "_", species, ".csv"))
write.csv(hub_genes, file = hub_genes_file, row.names = TRUE)

cat("✅ Hub genes for", target_module_color, "saved to:", hub_genes_file, "\n")
✅ Hub genes for green saved to: /Users/maevatecher/Documents/GitHub/locust-comparative-genomics/data/WGCNA/output/Bulk_RNAseq/gregaria/HubGenes_green_Thorax_gregaria.csv 

Visualing Hub Genes

# Check if we have any hub genes
if (nrow(hub_genes) > 0) {
    # Plot top 20 hub genes by module membership
    top_hub_genes <- hub_genes[order(-abs(hub_genes[[target_module_color]])), ][1:min(20, nrow(hub_genes)), ]

    # Save bar plot
    plot_file <- file.path(workDir, "WGCNA/output/Bulk_RNAseq", species, paste0("TopHubGenes_", target_module_color, "_", tissue, "_", species, ".pdf"))
    pdf(plot_file, width = 10, height = 8)

    barplot(
        abs(top_hub_genes[[target_module_color]]), 
        names.arg = rownames(top_hub_genes),
        las = 2, col = target_module_color,
        main = paste("Top Hub Genes in", target_module_color, "Module"),
        ylab = "Module Membership Score"
    )
    
    dev.off()
    
    cat("✅ Top hub genes plot saved to:", plot_file, "\n")
} else {
    cat("⚠️ No hub genes found in", target_module_color, "\n")
}
✅ Top hub genes plot saved to: /Users/maevatecher/Documents/GitHub/locust-comparative-genomics/data/WGCNA/output/Bulk_RNAseq/gregaria/TopHubGenes_green_Thorax_gregaria.pdf 

Extracting the adjacency matrix for the target module

# Ensure target module color is set
target_module_color <- "green"  # Change this as needed

# Extract genes belonging to the target module
target_genes <- names(net$colors)[net$colors == target_module_color]

# Check if module has enough genes
if (length(target_genes) < 2) {
    stop("❌ The selected module has too few genes for network extraction.")
}

# Make sure all module genes really are column names
if (! all(target_genes %in% colnames(datExpr)) ) {
  stop("Some turquoise genes not found in datExpr columns!")
}

# Subset columns = genes
datExpr_module <- datExpr[, target_genes, drop = FALSE]

# Quick sanity check
cat("✔️ datExpr_module dims (samples × genes):", dim(datExpr_module), "\n")
✔️ datExpr_module dims (samples × genes): 10 488 
# e.g.  40 samples ×  1500 module genes

# Now compute adjacency
adjacency_matrix <- adjacency(
  datExpr_module,
  power = picked_power,
  type  = "unsigned"
)

# And confirm
cat("✔️ adjacency_matrix dims:", dim(adjacency_matrix), "\n")
✔️ adjacency_matrix dims: 488 488 
# should be 1500 × 1500 (genes × genes)

# Compute TOM similarity **only for module genes**
TOM_module <- TOMsimilarity(adjacency_matrix)
..connectivity..
..matrix multiplication (system BLAS)..
..normalization..
..done.
# Verify dimensions
if (!all(dim(TOM_module) == length(target_genes))) {
  stop("❌ TOM matrix dimensions do not match target genes.")
}

# Assign proper row and column names
rownames(TOM_module) <- colnames(TOM_module) <- target_genes

# Save adjacency and TOM matrices
output_dir <- file.path(workDir, "WGCNA/output/Bulk_RNAseq", species)
dir.create(output_dir, showWarnings = FALSE, recursive = TRUE)

adj_matrix_file <- file.path(
  output_dir,
  paste0("AdjacencyMatrix_", target_module_color, "_", tissue, "_", species, ".csv")
)
TOM_file <- file.path(
  output_dir,
  paste0("TOMMatrix_", target_module_color, "_", tissue, "_", species, ".csv")
)

write.csv(adjacency_matrix, file = adj_matrix_file, row.names = TRUE)
write.csv(TOM_module,       file = TOM_file,          row.names = TRUE)

cat("✅ Adjacency and TOM matrices saved:\n",
    "   • Adjacency →", adj_matrix_file, "\n",
    "   • TOM       →", TOM_file, "\n")
✅ Adjacency and TOM matrices saved:
    • Adjacency → /Users/maevatecher/Documents/GitHub/locust-comparative-genomics/data/WGCNA/output/Bulk_RNAseq/gregaria/AdjacencyMatrix_green_Thorax_gregaria.csv 
    • TOM       → /Users/maevatecher/Documents/GitHub/locust-comparative-genomics/data/WGCNA/output/Bulk_RNAseq/gregaria/TOMMatrix_green_Thorax_gregaria.csv 
library(WGCNA)

# 1) Define module & genes
target_module_color <- "green"
modGenes <- names(net$colors)[net$colors == target_module_color]

# 2) Define where to write your Cytoscape files
output_dir <- file.path(workDir, "WGCNA/output/Bulk_RNAseq", species)
edgeFile   <- file.path(output_dir, paste0("Cytoscape_", target_module_color, "_edges.txt"))
nodeFile   <- file.path(output_dir, paste0("Cytoscape_", target_module_color, "_nodes.txt"))

# 3) Export using WGCNA helper
exportNetworkToCytoscape(
  TOM_module,
  edgeFile     = edgeFile,
  nodeFile     = nodeFile,
  weighted     = TRUE,
  threshold    = 0.005,             # adjust to keep a reasonable number of edges
  nodeNames    = modGenes,
  nodeAttr     = module_membership[modGenes, c(target_module_color, "ModuleColor")]
)
$edgeData
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LOC126298847 LOC126298847      NA  0.9278154       green
LOC126298317 LOC126298317      NA -0.7129224       green
LOC126297566 LOC126297566      NA  0.8042652       green
LOC126299231 LOC126299231      NA  0.4223831       green
LOC126299133 LOC126299133      NA  0.8981408       green
LOC126298666 LOC126298666      NA  0.7593294       green
LOC126297376 LOC126297376      NA  0.6956847       green
LOC126297640 LOC126297640      NA -0.8581729       green
LOC126298908 LOC126298908      NA -0.6519348       green
LOC126298356 LOC126298356      NA -0.6585644       green
LOC126297705 LOC126297705      NA -0.8059519       green
LOC126298173 LOC126298173      NA  0.6114772       green
LOC126299148 LOC126299148      NA  0.9522524       green
LOC126297514 LOC126297514      NA -0.9657490       green
LOC126298639 LOC126298639      NA -0.2653259       green
LOC126298572 LOC126298572      NA  0.9013904       green
LOC126297448 LOC126297448      NA  0.5838376       green
LOC126297434 LOC126297434      NA -0.8858845       green
LOC126298812 LOC126298812      NA  0.9160980       green
LOC126297731 LOC126297731      NA  0.6864641       green
LOC126299061 LOC126299061      NA -0.8906913       green
LOC126297865 LOC126297865      NA -0.4806383       green
LOC126297537 LOC126297537      NA  0.7178193       green
LOC126299121 LOC126299121      NA  0.9005812       green
LOC126299081 LOC126299081      NA  0.4252758       green
LOC126297822 LOC126297822      NA -0.6735150       green
LOC126298737 LOC126298737      NA  0.9359733       green
LOC126299621 LOC126299621      NA  0.8494388       green
LOC126297573 LOC126297573      NA  0.6284471       green
LOC126299201 LOC126299201      NA  0.9379011       green
LOC126298375 LOC126298375      NA  0.4912773       green
LOC126298423 LOC126298423      NA  0.2563585       green
LOC126299513 LOC126299513      NA  0.9084837       green
LOC126298498 LOC126298498      NA  0.9314622       green
LOC126297741 LOC126297741      NA  0.8160990       green
LOC126298763 LOC126298763      NA  0.8493490       green
LOC126298954 LOC126298954      NA  0.5788011       green
LOC126297542 LOC126297542      NA -0.9366254       green
LOC126297862 LOC126297862      NA -0.7611193       green
LOC126299537 LOC126299537      NA  0.4185444       green
LOC126297802 LOC126297802      NA  0.9093917       green
LOC126298372 LOC126298372      NA -0.4673658       green
LOC126297703 LOC126297703      NA  0.8619653       green
LOC126298676 LOC126298676      NA -0.7161711       green
LOC126301315 LOC126301315      NA  0.2765553       green
LOC126305057 LOC126305057      NA -0.6876831       green
LOC126305066 LOC126305066      NA -0.4677473       green
LOC126305264 LOC126305264      NA -0.8614438       green
LOC126311359 LOC126311359      NA -0.9155244       green
LOC126320374 LOC126320374      NA -0.5558147       green
LOC126320376 LOC126320376      NA -0.8671109       green
LOC126328317 LOC126328317      NA -0.8747045       green
# 4) Quick sanity check
if (!file.exists(edgeFile) || !file.exists(nodeFile)) {
  stop("❌ Cytoscape export failed: edge or node file not found.")
}
cat("✅ Cytoscape files ready:\n",
    "   • Edges →", edgeFile, "\n",
    "   • Nodes →", nodeFile, "\n")
✅ Cytoscape files ready:
    • Edges → /Users/maevatecher/Documents/GitHub/locust-comparative-genomics/data/WGCNA/output/Bulk_RNAseq/gregaria/Cytoscape_green_edges.txt 
    • Nodes → /Users/maevatecher/Documents/GitHub/locust-comparative-genomics/data/WGCNA/output/Bulk_RNAseq/gregaria/Cytoscape_green_nodes.txt 

Network within target module

library(WGCNA)
library(visNetwork)
library(scales)

# 0) Module to plot
target_module_color <- "green"

# 1) Genes in that module
modGenes <- names(net$colors)[ net$colors == target_module_color ]

# 2) Adjacency for those genes
datExpr_mod <- datExpr[, modGenes, drop = FALSE]
adj_full    <- adjacency(datExpr_mod, power = picked_power, type = "unsigned")

# 3) Threshold to top 1% edges
thr_edge   <- quantile(adj_full[upper.tri(adj_full)], 0.99)
adj_thresh <- adj_full
adj_thresh[ adj_full < thr_edge ] <- 0

# 4) Build edge dataframe
gene_names <- colnames(adj_thresh)
idx        <- which(adj_thresh > 0, arr.ind = TRUE)
edges_df   <- data.frame(
  from   = gene_names[ idx[,1] ],
  to     = gene_names[ idx[,2] ],
  weight = adj_thresh[ idx ],
  stringsAsFactors = FALSE
)

# 5) Precompute degrees for each gene
degree_mod <- rowSums(adj_thresh > 0)

# 6) Build node dataframe (fixed)
nodes_df <- data.frame(
  id     = modGenes,
  label  = modGenes,
  group  = net$colors[modGenes],                # module color
  value  = degree_mod[modGenes],                # node size
  title  = paste0(modGenes, "<br>Degree: ", degree_mod[modGenes]),
  stringsAsFactors = FALSE
)

# 7) Interactive network
visNetwork(nodes_df, edges_df, height = "700px", width = "100%") %>%
  visIgraphLayout(layout = "layout_with_fr") %>%
  visNodes(shape = "dot", shadow = TRUE) %>%
  visEdges(color = list(color = "grey80"), smooth = FALSE) %>%
  visOptions(highlightNearest = TRUE, nodesIdSelection = TRUE) %>%
  visLegend(useGroups = TRUE, position = "right", main = "Module")

Cross-tissues Gene Network Inference

library(WGCNA)
allowWGCNAThreads()
Allowing multi-threading with up to 12 threads.
options(stringsAsFactors = FALSE)

# ────────────────────────────────────────────────────────────────────
# 0) USER PARAMETER: change this to rerun for any other species
species <- "gregaria"
# ────────────────────────────────────────────────────────────────────

tissues        <- c("Head", "Thorax")
softPowerOpts  <- list()       # to store chosen β for each tissue
exprs          <- list()       # expression data (samples × genes)
moduleColors   <- list()       # module assignments for each tissue

# ────────────────────────────────────────────────────────────────────
# A) Load, orient, and numeric-ify each tissue’s datExpr
# ────────────────────────────────────────────────────────────────────
for(tiss in tissues) {
  path <- file.path(workDir, "WGCNA/input/Bulk_RNAseq",
                    paste0("datExpr_", tiss, "_", species, ".csv"))
  raw  <- read.csv(path, row.names = 1, check.names = FALSE, stringsAsFactors = FALSE)
  # Detect orientation: if rownames look like gene IDs (e.g. LOC…), then transpose
  if (grepl("^LOC", rownames(raw)[1]) || grepl("^ENS", rownames(raw)[1])) {
    dat <- t(raw)
  } else {
    dat <- raw
  }
  dat        <- as.data.frame(dat)
  dat[]      <- lapply(dat, as.numeric)
  exprs[[tiss]] <- dat
  
  # Quick check
  cat(sprintf("✔ Loaded %s: %d samples × %d genes\n",
              tiss, nrow(dat), ncol(dat)))
}
✔ Loaded Head: 12 samples × 12775 genes
✔ Loaded Thorax: 10 samples × 12361 genes
# ────────────────────────────────────────────────────────────────────
# B) For each tissue: pick a soft threshold, then detect modules
# ────────────────────────────────────────────────────────────────────
for(tiss in tissues) {
  dat   <- exprs[[tiss]]
  # 1) Soft‐thresholding analysis
  powers      <- c(1:10, seq(12,30,2))
  sft         <- pickSoftThreshold(dat, powerVector = powers, verbose = 0)
  beta        <- sft$powerEstimate
  if (is.na(beta)) {
    warning("No powerEstimate for ", tiss, "; defaulting to 6")
    beta <- 6
  }
  softPowerOpts[[tiss]] <- beta
  
  cat(sprintf(" ➔ %s: chosen soft‐power β = %d\n", tiss, beta))
  
  # 2) Module detection
  net <- blockwiseModules(
    dat,
    power           = beta,
    networkType     = "unsigned",
    minModuleSize   = 30,
    mergeCutHeight  = 0.20,
    numericLabels   = FALSE,
    verbose         = 0
  )
  moduleColors[[tiss]] <- net$colors
  tbl <- table(net$colors)
  cat(sprintf(" ➔ %s: detected %d modules (incl. grey)\n\n",
              tiss, length(tbl)))
}
   Power SFT.R.sq  slope truncated.R.sq mean.k. median.k. max.k.
1      1   0.0283  0.251          0.574 4360.00  4340.000   6560
2      2   0.4170 -0.640          0.766 2130.00  2000.000   4280
3      3   0.6960 -0.964          0.860 1220.00  1060.000   3080
4      4   0.7850 -1.110          0.904  773.00   615.000   2340
5      5   0.8060 -1.210          0.909  522.00   378.000   1850
6      6   0.8290 -1.270          0.926  370.00   244.000   1500
7      7   0.8310 -1.330          0.929  272.00   163.000   1240
8      8   0.8430 -1.360          0.941  205.00   112.000   1040
9      9   0.8470 -1.390          0.947  159.00    79.300    891
10    10   0.8590 -1.410          0.957  125.00    57.600    767
11    12   0.8590 -1.460          0.960   81.80    31.800    585
12    14   0.8600 -1.490          0.964   56.00    18.700    458
13    16   0.8610 -1.510          0.968   39.90    11.500    367
14    18   0.8670 -1.520          0.974   29.30     7.420    299
15    20   0.8770 -1.530          0.981   22.10     4.940    247
16    22   0.8810 -1.540          0.985   17.00     3.370    206
17    24   0.8890 -1.540          0.988   13.30     2.330    174
18    26   0.8950 -1.540          0.991   10.60     1.670    149
19    28   0.8980 -1.540          0.992    8.54     1.220    128
20    30   0.9010 -1.540          0.994    6.97     0.916    111
 ➔ Head: chosen soft‐power β = 10
 ➔ Head: detected 64 modules (incl. grey)

   Power SFT.R.sq  slope truncated.R.sq mean.k. median.k. max.k.
1      1    0.262  0.822          0.373  4550.0   4490.00   6560
2      2    0.227 -0.317          0.236  2400.0   2180.00   4540
3      3    0.813 -0.690          0.790  1480.0   1210.00   3480
4      4    0.923 -0.859          0.907  1010.0    729.00   2810
5      5    0.947 -0.950          0.935   729.0    480.00   2350
6      6    0.953 -1.000          0.942   551.0    330.00   2010
7      7    0.954 -1.040          0.945   430.0    235.00   1750
8      8    0.949 -1.080          0.941   344.0    170.00   1550
9      9    0.943 -1.110          0.938   282.0    125.00   1390
10    10    0.935 -1.130          0.931   234.0     94.60   1250
11    12    0.935 -1.160          0.937   169.0     56.70   1040
12    14    0.925 -1.190          0.931   127.0     35.80    882
13    16    0.918 -1.210          0.928    98.0     23.60    761
14    18    0.915 -1.220          0.929    77.8     16.10    665
15    20    0.912 -1.240          0.930    63.0     11.40    587
16    22    0.914 -1.240          0.934    51.9      8.24    523
17    24    0.910 -1.250          0.934    43.4      6.09    469
18    26    0.911 -1.260          0.937    36.7      4.60    423
19    28    0.915 -1.260          0.942    31.3      3.54    384
20    30    0.918 -1.270          0.946    27.0      2.78    351
 ➔ Thorax: chosen soft‐power β = 4
 ➔ Thorax: detected 79 modules (incl. grey)
# Show a quick summary
print(softPowerOpts)
$Head
[1] 10

$Thorax
[1] 4
print(lapply(moduleColors, table))
$Head

  antiquewhite4         bisque4           black            blue           brown 
             34              53             486            1391             977 
         brown4          coral1          coral2            cyan       darkgreen 
             54              35              34             132              92 
       darkgrey     darkmagenta  darkolivegreen      darkorange     darkorange2 
             86              68              70              81              54 
        darkred   darkseagreen4   darkslateblue   darkturquoise     floralwhite 
             96              35              52              90              55 
          green     greenyellow            grey          grey60       honeydew1 
            729             156            1103             103              35 
          ivory  lavenderblush3       lightcyan      lightcyan1      lightgreen 
             56              36             111              58             103 
     lightpink4 lightsteelblue1     lightyellow         magenta          maroon 
             37              60             103             183              41 
   mediumorchid   mediumpurple3    midnightblue    navajowhite2          orange 
             31              63             123              43              84 
     orangered4   paleturquoise  palevioletred3            pink           plum1 
             64              72              44             214              64 
          plum2          purple             red       royalblue     saddlebrown 
             49             159             692              96              77 
         salmon         salmon4         sienna3         skyblue        skyblue3 
            141              47              68              77              65 
      steelblue             tan        thistle1        thistle2       turquoise 
             76             149              48              48            2149 
         violet           white          yellow     yellowgreen 
             72              80             924              67 

$Thorax

  antiquewhite4         bisque4           black            blue           blue2 
             43              53             244            1189              36 
          brown          brown2          brown4          coral1          coral2 
            991              36              56              44              43 
           cyan       darkgreen        darkgrey     darkmagenta  darkolivegreen 
            133              99              89              66              67 
darkolivegreen4      darkorange     darkorange2         darkred   darkseagreen4 
             36              85              59             102              44 
  darkslateblue   darkturquoise      darkviolet      firebrick4     floralwhite 
             52              91              32              37              59 
          green     greenyellow            grey          grey60       honeydew1 
            332             144              26             118              44 
     indianred4           ivory  lavenderblush3      lightcoral       lightcyan 
             38              59              45              40             125 
     lightcyan1      lightgreen      lightpink4  lightsteelblue lightsteelblue1 
             59             118              45              40              61 
    lightyellow         magenta          maroon    mediumorchid   mediumpurple2 
            111             187              45              43              40 
  mediumpurple3    midnightblue    navajowhite2          orange      orangered3 
             61             131              46              85              40 
     orangered4   paleturquoise  palevioletred3            pink            plum 
             62              78              46             208              41 
          plum1           plum2           plum3          purple             red 
             63              51              31             171             331 
      royalblue     saddlebrown          salmon         salmon4         sienna3 
            102              80             134              47              64 
        skyblue        skyblue1        skyblue2        skyblue3       steelblue 
             82              41              43              63              78 
            tan        thistle1        thistle2       turquoise          violet 
            140              49              50            3759              69 
          white          yellow         yellow4     yellowgreen 
             82             422              41              64 
# ────────────────────────────────────────────────────────────────────
# C) Module preservation: Head (ref) → Thorax (test)
# ────────────────────────────────────────────────────────────────────
multiExpr <- list(
  Head   = list(data = exprs[["Head"]]),
  Thorax = list(data = exprs[["Thorax"]])
)
multiColor <- list(
  Head   = moduleColors[["Head"]],
  Thorax = moduleColors[["Thorax"]]
)

mp <- modulePreservation(
  multiExpr, multiColor,
  referenceNetworks = 1,
  nPermutations     = 200,
  networkType        = "unsigned",
  verbose            = 0
)

# After running mp <- modulePreservation(...)
# 1) Find the indices of Head (reference) and Thorax (test) in the list
refIndex  <- 1              # Head is first in multiExpr
testIndex <- 2              # Thorax is second

# 2) Extract the Z‐stat matrix and the observed‐stat matrix
Zmat  <- mp$preservation$Z[[ refIndex ]][[ testIndex ]]
Omat  <- mp$preservation$observed[[ refIndex ]][[ testIndex ]]

# 3) Pull out the columns you want
Zsummary   <- Zmat[ , "Zsummary.pres" ]        # Z‐summary preservation
medianRank <- Omat[ , "medianRank.pres" ]      # median‐rank preservation

# 4) Get module names
mods <- rownames(Zmat)

# 5) Plot them
par(mfrow = c(1,2), mar = c(6,4,3,1))

# 1) Z‐summary plot
x <- seq_along(Zsummary)
plot(
  x, Zsummary,
  pch    = 21,
  bg     = mods,
  cex    = 2,                        # bigger dots
  xaxt   = "n",
  xlab   = "",
  ylab   = "Z-summary preservation",
  main   = paste(species, "Head → Thorax (Zsummary)")
)
axis(1, at = x, labels = mods, las = 2, cex.axis = 0.8)
abline(h = 2,  col = "blue",  lty = 2)   # moderate
abline(h = 10, col = "red",   lty = 2)   # strong

# label modules with Zsummary > 10
highZ <- which(Zsummary > 10)
text(
  x[highZ], Zsummary[highZ],
  labels = mods[highZ],
  pos    = 3,       # above
  cex    = 0.8,
  font   = 2        # bold
)

# 2) Median‐rank plot
x2 <- seq_along(medianRank)
plot(
  x2, medianRank,
  pch    = 21,
  bg     = mods,
  cex    = 2,                        # bigger dots
  xaxt   = "n",
  xlab   = "",
  ylab   = "Median rank preservation",
  main   = paste(species, "Head → Thorax (Median rank)")
)
axis(1, at = x2, labels = mods, las = 2, cex.axis = 0.8)
abline(
  h = mean(medianRank, na.rm = TRUE),
  col = "darkgreen", lty = 2
)

# label modules with medianRank < overall mean (best preserved)
lowMr <- which(medianRank < mean(medianRank, na.rm = TRUE))
text(
  x2[lowMr], medianRank[lowMr],
  labels = mods[lowMr],
  pos    = 3,
  cex    = 0.8,
  font   = 2
)

Version Author Date
a2d2955 Maeva TECHER 2025-07-01
par(mfrow = c(1,1))

What it shows: for each module, the Z-summary statistic quantifies how well the connectivity patterns (topology) of that module in Head are preserved in Thorax.

Z-summary plot:

  • Z = 10 (red): modules above this are strongly preserved.
  • Z = 2 (blue): modules between 2 and 10 are moderately preserved.
  • Below 2: little to no evidence of preservation.

Median-rank plot:

The plot shows the median rank of preservation statistics for each module, for which lower value is is better. The dashed line indicates the average median-rank across all modules. Modules with rank below this line are better preserved.

Network comparison across Schistocerca

Data loading and preprocessing

We will be using the BioNERO R package and will follow the tutorial to: - Infer gene co-expression network across tissues within a species. - Infer regulatory network across tissues within a species. - Compare networks across species

library(BioNERO)
library(SummarizedExperiment)
library(S4Vectors)
set.seed(456) # for reproducibility

# A) Your list of species
species_list <- c(
  "gregaria",
  "americana",
  "cancellata",
  "piceifrons",
  "cubense",
  "nitens"
)

# B) Loop
for (species in species_list) {
  message("↪ Processing species: ", species)
  
  # 1) Paths
  head_file   <- file.path(
    workDir, "WGCNA/input/Bulk_RNAseq",
    paste0("datExpr_Head_",   species, ".csv")
  )
  thorax_file <- file.path(
    workDir, "WGCNA/input/Bulk_RNAseq",
    paste0("datExpr_Thorax_", species, ".csv")
  )
  
  # 2) Read (rows=samples, cols=genes)
  head_df   <- read.csv(head_file,   row.names = 1, check.names = FALSE)
  thorax_df <- read.csv(thorax_file, row.names = 1, check.names = FALSE)
  
  # 3) Transpose → (genes×samples)
  head_mat   <- t(as.matrix(head_df))
  thorax_mat <- t(as.matrix(thorax_df))
  
  # 4) Build per‐tissue SEs
  for (tiss in c("Head","Thorax")) {
    mat <- if (tiss=="Head") head_mat else thorax_mat
    samples <- colnames(mat)
    coldata <- DataFrame(
      Sample  = samples,
      Tissue  = factor(tiss, levels=c("Head","Thorax")),
      Species = factor(species, levels=species_list),
      row.names = samples
    )
    se <- SummarizedExperiment(
      assays  = SimpleList(vst = mat),
      colData = coldata,
      rowData = DataFrame(GeneID = rownames(mat))
    )
    out_file <- file.path(
      workDir, "WGCNA/input/Bulk_RNAseq",
      paste0("SE_", tiss, "_", gsub(" ", "_", species), ".rds")
    )
    saveRDS(se, file = out_file)
    message("  • Saved ", basename(out_file))
  }
  
  # 5) Combined Head+Thorax
  common_genes <- intersect(rownames(head_mat), rownames(thorax_mat))
  head_mat_c   <- head_mat[common_genes, , drop=FALSE]
  thorax_mat_c <- thorax_mat[common_genes, , drop=FALSE]
  comb_mat     <- cbind(head_mat_c, thorax_mat_c)
  
  samples  <- colnames(comb_mat)
  nHead    <- ncol(head_mat_c)
  nThorax  <- ncol(thorax_mat_c)
  coldata  <- DataFrame(
    Sample  = samples,
    Tissue  = factor(
                 c(rep("Head",   nHead),
                   rep("Thorax", nThorax)),
                 levels=c("Head","Thorax")
               ),
    Species = factor(rep(species, length(samples)), levels=species_list),
    row.names = samples
  )
  
  se_comb <- SummarizedExperiment(
    assays  = SimpleList(vst = comb_mat),
    colData = coldata,
    rowData = DataFrame(GeneID = common_genes)
  )
  out_file <- file.path(
    workDir, "WGCNA/input/Bulk_RNAseq",
    paste0("SE_HeadThorax_", gsub(" ", "_", species), ".rds")
  )
  saveRDS(se_comb, file = out_file)
  message("  • Saved ", basename(out_file), "\n")
}

We then will remove the missing values if any, non-expressed genes:

# ───────────────────────────────────────────────────────────
# 1) Load your SummarizedExperiment (Head / Thorax combined)
# ───────────────────────────────────────────────────────────
se_file   <- file.path(
  workDir, "WGCNA/input/Bulk_RNAseq",
  "SE_Head_gregaria.rds"
)
final_exp <- readRDS(se_file)

# ───────────────────────────────────────────────────────────
# 2) (Optional) Preprocess for WGCNA
# ───────────────────────────────────────────────────────────
# final_exp <- exp_preprocess(
#   final_exp,
#   min_exp         = 5,
#   variance_filter = TRUE,
#   n               = 10000
# )
print(final_exp)
class: SummarizedExperiment 
dim: 12775 12 
metadata(0):
assays(1): vst
rownames(12775): LOC126318536 LOC126318656 ... LOC126328317
  LOC126330722
rowData names(1): GeneID
colnames(12): SGRE-HEAD-CRD-1 SGRE-HEAD-CRD-2 ... SGRE-HEAD-ISO-5
  SGRE-HEAD-ISO-6
colData names(3): Sample Tissue Species
# ───────────────────────────────────────────────────────────
# 3) Import behavioral traits into colData
# ───────────────────────────────────────────────────────────
behavior_file <- file.path(
  workDir, "behavioral_data", "Sample_phenotypes_WGCNA.csv"
)
behavior_df <- read.csv(behavior_file, stringsAsFactors = FALSE)

# set rownames to SampleName for easy subsetting
rownames(behavior_df) <- behavior_df$SampleName

# define which columns are your traits
trait_cols <- c(
  "RearingCondition",
  "MovedDistance",
  "StimulusZoneTime",
  "NonStimulusZoneTime",
  "TimeStimulusWall"
)

# subset & reorder to match final_exp samples
meta_sub <- behavior_df[colnames(final_exp), trait_cols, drop = FALSE]

# re‐initialize colData to only the core columns
cd0 <- colData(final_exp)[, c("Sample", "Tissue", "Species")]

# add each trait without duplication
for (col in trait_cols) {
  if (col == "RearingCondition") {
    # categorical
    cd0[[col]] <- factor(
      meta_sub[[col]],
      levels = c("Isolated", "Crowded")
    )
  } else {
    # continuous
    cd0[[col]] <- as.numeric(meta_sub[[col]])
  }
}

# overwrite colData with this clean version
colData(final_exp) <- DataFrame(cd0)

# sanity check
head(colData(final_exp))
DataFrame with 6 rows and 8 columns
                         Sample   Tissue  Species RearingCondition
                    <character> <factor> <factor>         <factor>
SGRE-HEAD-CRD-1 SGRE-HEAD-CRD-1     Head gregaria          Crowded
SGRE-HEAD-CRD-2 SGRE-HEAD-CRD-2     Head gregaria          Crowded
SGRE-HEAD-CRD-3 SGRE-HEAD-CRD-3     Head gregaria          Crowded
SGRE-HEAD-CRD-4 SGRE-HEAD-CRD-4     Head gregaria          Crowded
SGRE-HEAD-CRD-5 SGRE-HEAD-CRD-5     Head gregaria          Crowded
SGRE-HEAD-CRD-6 SGRE-HEAD-CRD-6     Head gregaria          Crowded
                MovedDistance StimulusZoneTime NonStimulusZoneTime
                    <numeric>        <numeric>           <numeric>
SGRE-HEAD-CRD-1       132.673          220.447             109.832
SGRE-HEAD-CRD-2       132.673          220.447             109.832
SGRE-HEAD-CRD-3       132.673          220.447             109.832
SGRE-HEAD-CRD-4       132.673          220.447             109.832
SGRE-HEAD-CRD-5       132.673          220.447             109.832
SGRE-HEAD-CRD-6       132.673          220.447             109.832
                TimeStimulusWall
                       <numeric>
SGRE-HEAD-CRD-1          119.282
SGRE-HEAD-CRD-2          119.282
SGRE-HEAD-CRD-3          119.282
SGRE-HEAD-CRD-4          119.282
SGRE-HEAD-CRD-5          119.282
SGRE-HEAD-CRD-6          119.282

Gene coexpression network inference

Assessing module stability

# ───────────────────────────────────────────────────────────
# 2) Infer the network as before…
# ───────────────────────────────────────────────────────────
sft   <- SFT_fit(final_exp, net_type = "unsigned", cor_method = "pearson")
   Power SFT.R.sq  slope truncated.R.sq mean.k. median.k. max.k.
1      3    0.696 -0.964          0.860  1220.0   1060.00   3080
2      4    0.785 -1.110          0.904   773.0    615.00   2340
3      5    0.806 -1.210          0.909   522.0    378.00   1850
4      6    0.829 -1.270          0.926   370.0    244.00   1500
5      7    0.831 -1.330          0.929   272.0    163.00   1240
6      8    0.843 -1.360          0.941   205.0    112.00   1040
7      9    0.847 -1.390          0.947   159.0     79.30    891
8     10    0.859 -1.410          0.957   125.0     57.60    767
9     11    0.863 -1.430          0.962   101.0     42.40    667
10    12    0.859 -1.460          0.960    81.8     31.80    585
11    13    0.858 -1.470          0.962    67.3     24.30    516
12    14    0.860 -1.490          0.964    56.0     18.70    458
13    15    0.860 -1.510          0.967    47.1     14.60    409
14    16    0.861 -1.510          0.968    39.9     11.50    367
15    17    0.865 -1.520          0.971    34.1      9.20    330
16    18    0.867 -1.520          0.974    29.3      7.42    299
17    19    0.872 -1.530          0.978    25.4      6.03    271
18    20    0.877 -1.530          0.981    22.1      4.94    247
sft$plot

Version Author Date
a2d2955 Maeva TECHER 2025-07-01
power <- sft$power
net   <- exp2gcn(
  final_exp,
  net_type  = "unsigned",
  SFTpower  = 6,
  cor_method = "pearson"
)
..connectivity..
..matrix multiplication (system BLAS)..
..normalization..
..done.
names(net)
[1] "adjacency_matrix"    "MEs"                 "genes_and_modules"  
[4] "kIN"                 "correlation_matrix"  "params"             
[7] "dendro_plot_objects"
plot_dendro_and_colors(net)

Version Author Date
a2d2955 Maeva TECHER 2025-07-01
# Eigengene networks
plot_eigengene_network(net)

Version Author Date
a2d2955 Maeva TECHER 2025-07-01
plot_ngenes_per_module(net)

Version Author Date
a2d2955 Maeva TECHER 2025-07-01
module_stability(final_exp, net, nRuns = 10)
 ...working on run 1 ..
 ...working on run 2 ..
 ...working on run 3 ..
 ...working on run 4 ..
 ...working on run 5 ..
 ...working on run 6 ..
 ...working on run 7 ..
 ...working on run 8 ..
 ...working on run 9 ..
 ...working on run 10 ..
 ...working on run 11 ..

Version Author Date
329b590 Maeva TECHER 2025-07-01
a2d2955 Maeva TECHER 2025-07-01

Module-trait associations

# ───────────────────────────────────────────────────────────
# 6) Module–Trait associations
# ───────────────────────────────────────────────────────────
MEtrait <- module_trait_cor(
  exp           = final_exp,
  MEs           = net$MEs,
  metadata_cols = trait_cols
)
head(MEtrait)
               ME    trait         cor       pvalue            group
1     MEaliceblue  Crowded -0.97339612 1.003740e-07 RearingCondition
2     MEaliceblue Isolated  0.97339612 1.003740e-07 RearingCondition
3  MEantiquewhite  Crowded -0.76772497 3.550596e-03 RearingCondition
4  MEantiquewhite Isolated  0.76772497 3.550596e-03 RearingCondition
5 MEantiquewhite1  Crowded -0.02770547 9.318883e-01 RearingCondition
6 MEantiquewhite1 Isolated  0.02770547 9.318883e-01 RearingCondition
plot_module_trait_cor(MEtrait)

Version Author Date
a2d2955 Maeva TECHER 2025-07-01
plot_expression_profile(
    exp = final_exp, 
    metadata_cols = "RearingCondition",
    net = net, 
    plot_module = TRUE, 
    modulename = "aliceblue"
)

Version Author Date
a2d2955 Maeva TECHER 2025-07-01

Hub gene identification

hubs <- get_hubs_gcn(final_exp, net)
head(hubs)
          Gene    Module  kWithin
1 LOC126355959 aliceblue 519.3610
2 LOC126299042 aliceblue 514.1214
3 LOC126334624 aliceblue 512.9888
4 LOC126283985 aliceblue 511.1058
5 LOC126297619 aliceblue 510.8234
6 LOC126272084 aliceblue 507.1924
edges <- get_edge_list(net, module="aliceblue")
head(edges)
            Gene1        Gene2     Weight
2221 LOC126319460 LOC126320326 -0.6046428
4441 LOC126319460 LOC126320407  0.7492392
4442 LOC126320326 LOC126320407 -0.5617575
6661 LOC126319460 LOC126320662  0.5943654
6662 LOC126320326 LOC126320662 -0.4490141
6663 LOC126320407 LOC126320662  0.6385784
# Remove edges based on optimal scale-free topology fit
edges_filtered <- get_edge_list(net, module = "aliceblue", filter = TRUE)

dim(edges_filtered)
[1] 21574     3
edges_filtered <- get_edge_list(
    net, module = "aliceblue",
    filter = TRUE, method = "pvalue", 
    nSamples = ncol(final_exp)
)
dim(edges_filtered)
[1] 1177405       3
edges_filtered <- get_edge_list(
    net, module = "aliceblue", 
    filter = TRUE, method = "min_cor", rcutoff = 0.7
)
dim(edges_filtered)
[1] 329122      3
plot_gcn(
    edgelist_gcn = edges_filtered, 
    net = net, 
    color_by = "module", 
    hubs = hubs
)

Version Author Date
a2d2955 Maeva TECHER 2025-07-01
library(networkD3)

#plot_gcn(
#    edgelist_gcn = edges_filtered, 
#    net = net,
#    color_by = "module",
#    hubs = hubs,
#    interactive = TRUE,
#    dim_interactive = c(500, 500)
#)

Gene regulatory network inference

{Put the steps Emily did with Pfam}

Consensus GRN inference

We obtained a table that contains protein ID and TFs target. Before using the GeneID as a list for input in WGCNA, we will extract the GeneID (LOCXXX) with our Orthotable (see Orthology section), but we could also simply parse the .gff if needed. We then only extract the TFs that are expressed and present in our dataset.

ortho_dir <- "/Users/maevatecher/Documents/GitHub/locust-comparative-genomics/data/orthofinder/Polyneoptera"
input_file <- file.path(ortho_dir, "Results_I2_iqtree/Orthogroups_genesproteinbiotype_13species_annotated_May2025.csv")
ortho_map <- read.csv(input_file, header = TRUE, stringsAsFactors = FALSE)
head(ortho_map)
  Orthogroup                 SpeciesID     protein_id       GeneID
1  OG0000000 Asimp_filteredTranscripts XP_067003642.2 LOC136874043
2  OG0000000 Asimp_filteredTranscripts XP_067004661.1 LOC136874869
3  OG0000000 Asimp_filteredTranscripts XP_067015293.1 LOC136886419
4  OG0000000 Asimp_filteredTranscripts XP_067015651.2 LOC136886746
5  OG0000000 Asimp_filteredTranscripts XP_068085770.1 LOC137496902
6  OG0000000 Asimp_filteredTranscripts XP_068087037.1 LOC137503369
                                   Description Species       GeneType
1            farnesol dehydrogenase isoform X1   Asimp protein-coding
2 dehydrogenase/reductase SDR family member 11   Asimp protein-coding
3                       farnesol dehydrogenase   Asimp protein-coding
4                       farnesol dehydrogenase   Asimp protein-coding
5                  farnesol dehydrogenase-like   Asimp protein-coding
6                  farnesol dehydrogenase-like   Asimp protein-coding
    Accession     Begin       End Orthogroup_Type
1 NC_090269.1 316521124 316596183       MultiCopy
2 NC_090269.1 316408775 316497937       MultiCopy
3 NC_090279.1 165240845 165292312       MultiCopy
4 NC_090279.1 164532314 164617967       MultiCopy
5 NC_090275.1  60074551  60101839       MultiCopy
6 NC_090279.1 164618824 164719067       MultiCopy
gregaria_TF <- read.csv("/Users/maevatecher/Documents/GitHub/locust-comparative-genomics/data/WGCNA/input/GRNs/gregaria_TFfiltered_data.csv") 

# Step 2: Match protein_id (XP_) to query_name
regulator_map <- dplyr::inner_join(
  gregaria_TF,
  ortho_map,
  by = c("query_name" = "protein_id")
)

# Step 3: Extract the LOCID (GeneID) and optionally the family
regulators <- regulator_map %>%
  dplyr::select(GeneID, target_name) %>%
  dplyr::distinct() %>%
  dplyr::rename(Family = target_name)

regulatorsID <- unique(regulators$GeneID)
length(regulatorsID)
[1] 590
# Optional: intersect to ensure they're in the expression matrix
regulators <- intersect(regulatorsID, rownames(final_exp))
length(regulators)
[1] 449

Now we have the SummarizedExperiment and the TFs list, we will run the Gene Regulatory Network inference with BioNERO.

# Run BioNERO GRN inference
grn <- exp2grn(
    exp = final_exp, 
    regulators = regulatorsID
)

hubs <- get_hubs_grn(grn)
head(hubs)
          Gene Degree
1 LOC126291635     21
2 LOC126353797     21
3 LOC126354904     20
4 LOC126354461     20
5 LOC126336872     20
6 LOC126356196     19
plot_grn(grn, top_n_hubs = 10)

plot_grn(grn, interactive = TRUE, dim_interactive = c(1000,1000))

sessionInfo()
R version 4.4.2 (2024-10-31)
Platform: aarch64-apple-darwin20
Running under: macOS Sequoia 15.7.1

Matrix products: default
BLAS:   /Library/Frameworks/R.framework/Versions/4.4-arm64/Resources/lib/libRblas.0.dylib 
LAPACK: /Library/Frameworks/R.framework/Versions/4.4-arm64/Resources/lib/libRlapack.dylib;  LAPACK version 3.12.0

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

time zone: Asia/Tokyo
tzcode source: internal

attached base packages:
[1] stats4    stats     graphics  grDevices utils     datasets  methods  
[8] base     

other attached packages:
 [1] networkD3_0.4.1             SummarizedExperiment_1.36.0
 [3] Biobase_2.66.0              GenomicRanges_1.58.0       
 [5] GenomeInfoDb_1.42.3         IRanges_2.40.1             
 [7] S4Vectors_0.44.0            BiocGenerics_0.52.0        
 [9] MatrixGenerics_1.18.1       matrixStats_1.5.0          
[11] BioNERO_1.14.0              scales_1.4.0               
[13] visNetwork_2.1.3            cowplot_1.1.3              
[15] ggridges_0.5.6              WGCNA_1.73                 
[17] fastcluster_1.3.0           dynamicTreeCut_1.63-1      
[19] kableExtra_1.4.0            ggrepel_0.9.6              
[21] ggpubr_0.6.0                RColorBrewer_1.1-3         
[23] reshape2_1.4.4              ggthemes_5.1.0             
[25] plotly_4.11.0               DT_0.33                    
[27] data.table_1.17.6           lubridate_1.9.4            
[29] forcats_1.0.0               stringr_1.5.1              
[31] dplyr_1.1.4                 purrr_1.0.4                
[33] readr_2.1.5                 tidyr_1.3.1                
[35] tibble_3.3.0                ggplot2_3.5.2              
[37] tidyverse_2.0.0             rmdformats_1.0.4           
[39] knitr_1.50                 

loaded via a namespace (and not attached):
  [1] splines_4.4.2           later_1.4.2             GENIE3_1.28.0          
  [4] ggnetwork_0.5.13        preprocessCore_1.68.0   XML_3.99-0.18          
  [7] rpart_4.1.24            lifecycle_1.0.4         minet_3.64.0           
 [10] rstatix_0.7.2           edgeR_4.4.2             doParallel_1.0.17      
 [13] rprojroot_2.0.4         MASS_7.3-65             lattice_0.22-7         
 [16] crosstalk_1.2.1         backports_1.5.0         magrittr_2.0.3         
 [19] limma_3.62.2            Hmisc_5.2-3             sass_0.4.10            
 [22] rmarkdown_2.29          jquerylib_0.1.4         yaml_2.3.10            
 [25] httpuv_1.6.16           DBI_1.2.3               abind_1.4-8            
 [28] zlibbioc_1.52.0         nnet_7.3-20             sva_3.54.0             
 [31] git2r_0.36.2            circlize_0.4.16         GenomeInfoDbData_1.2.13
 [34] data.tree_1.1.0         genefilter_1.88.0       annotate_1.84.0        
 [37] svglite_2.2.1           DelayedArray_0.32.0     codetools_0.2-20       
 [40] xml2_1.3.8              tidyselect_1.2.1        shape_1.4.6.1          
 [43] UCSC.utils_1.2.0        farver_2.1.2            base64enc_0.1-3        
 [46] jsonlite_2.0.0          GetoptLong_1.0.5        Formula_1.2-5          
 [49] survival_3.8-3          iterators_1.0.14        systemfonts_1.2.3      
 [52] foreach_1.5.2           tools_4.4.2             Rcpp_1.0.14            
 [55] glue_1.8.0              SparseArray_1.6.2       gridExtra_2.3          
 [58] mgcv_1.9-3              xfun_0.52               withr_3.0.2            
 [61] fastmap_1.2.0           digest_0.6.37           timechange_0.3.0       
 [64] R6_2.6.1                textshaping_1.0.1       colorspace_2.1-1       
 [67] Cairo_1.6-2             GO.db_3.20.0            dichromat_2.0-0.1      
 [70] RSQLite_2.4.1           RhpcBLASctl_0.23-42     generics_0.1.4         
 [73] httr_1.4.7              htmlwidgets_1.6.4       S4Arrays_1.6.0         
 [76] whisker_0.4.1           pkgconfig_2.0.3         gtable_0.3.6           
 [79] blob_1.2.4              workflowr_1.7.1         ComplexHeatmap_2.22.0  
 [82] impute_1.80.0           XVector_0.46.0          htmltools_0.5.8.1      
 [85] carData_3.0-5           bookdown_0.43           clue_0.3-66            
 [88] png_0.1-8               ggdendro_0.2.0          NetRep_1.2.7           
 [91] rstudioapi_0.17.1       tzdb_0.5.0              rjson_0.2.23           
 [94] nlme_3.1-168            coda_0.19-4.1           statnet.common_4.12.0  
 [97] checkmate_2.3.2         cachem_1.1.0            GlobalOptions_0.1.2    
[100] parallel_4.4.2          foreign_0.8-90          AnnotationDbi_1.68.0   
[103] pillar_1.10.2           grid_4.4.2              vctrs_0.6.5            
[106] promises_1.3.3          car_3.1-3               xtable_1.8-4           
[109] cluster_2.1.8.1         htmlTable_2.4.3         evaluate_1.0.4         
[112] magick_2.8.7            locfit_1.5-9.12         cli_3.6.5              
[115] compiler_4.4.2          rlang_1.1.6             crayon_1.5.3           
[118] ggsignif_0.6.4          intergraph_2.0-4        labeling_0.4.3         
[121] plyr_1.8.9              fs_1.6.6                stringi_1.8.7          
[124] viridisLite_0.4.2       network_1.19.0          BiocParallel_1.40.2    
[127] Biostrings_2.74.1       lazyeval_0.2.2          Matrix_1.7-3           
[130] patchwork_1.3.1         hms_1.1.3               bit64_4.6.0-1          
[133] KEGGREST_1.46.0         statmod_1.5.0           igraph_2.1.4           
[136] broom_1.0.8             memoise_2.0.1           bslib_0.9.0            
[139] bit_4.6.0