Last updated: 2022-11-09

Checks: 6 1

Knit directory: dgrp-starve/

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Rmd 81037cc nklimko 2022-11-09 html reworking, snp success
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Rmd 41de4a8 nklimko 2022-11-08 difMinus success, reframe for all four genotype analysis

Phase 1

Full code

Data was extracted from two tables of eQTL male/female traits.

First, each file was filtered for non-null values

  # csvPath dependent upon female/male file
  allRaw <- tibble(read.csv(csvPath))
  starveRaw <- allRaw %>% select(line,starvation) %>% filter(!is.na(starvation))

Two tables, starveF and starveM, were then joined one. Difference and average of each line were calculated and added

#Combine mf
starve <- starveF %>% mutate(m = starveM$m)

#compute difference and average
starve <- starve %>% select(line, f, m) %>% mutate(dif = f - m, avg = (f+m)/2)

Phase 2

Full code

Lines were manually selected and hard coded into the following extractions.

#print(arrange(starve, starve$dif), n=205)
#print(arrange(starve, starve$avg), n=205)

# Lines with male SR high than female SR
difMinus <- starve %>% filter(dif < 0) %>% arrange(line) %>% select(line)
difMinus <- as.data.table(difMinus)
fwrite(difMinus, "./data/difMinus.txt")

# Lines with largest gap from male SR to female SR
difPlus <- starve %>% filter(dif >= 30) %>% arrange(line) %>% select(line)
difPlus <- as.data.table(difPlus)
fwrite(difPlus, "./data/difPlus.txt")

# Lowest average SR between males and females
avgMinus <- starve %>% filter(avg < 40) %>% arrange(line) %>% select(line)
avgMinus <- as.data.table(avgMinus)
fwrite(avgMinus, "./data/avgMinus.txt")

# Highest average SR between males and females
avgPlus <- starve %>% filter(dif >= 30) %>% arrange(line) %>% select(line)
avgPlus <- as.data.table(avgPlus)
fwrite(avgPlus, "./data/avgPlus.txt")

Phase 3

Full code

After creating the groups of interest, I


sessionInfo()
R version 4.0.3 (2020-10-10)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: CentOS Linux 7 (Core)

Matrix products: default
BLAS/LAPACK: /opt/ohpc/pub/Software/openblas_0.3.10/lib/libopenblas_haswellp-r0.3.10.dev.so

locale:
 [1] LC_CTYPE=en_US.utf-8       LC_NUMERIC=C              
 [3] LC_TIME=en_US.utf-8        LC_COLLATE=en_US.utf-8    
 [5] LC_MONETARY=en_US.utf-8    LC_MESSAGES=en_US.utf-8   
 [7] LC_PAPER=en_US.utf-8       LC_NAME=C                 
 [9] LC_ADDRESS=C               LC_TELEPHONE=C            
[11] LC_MEASUREMENT=en_US.utf-8 LC_IDENTIFICATION=C       

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
[1] data.table_1.14.2 dplyr_1.0.8       tidyr_1.2.0       workflowr_1.7.0  

loaded via a namespace (and not attached):
 [1] Rcpp_1.0.8.3     bslib_0.3.1      jquerylib_0.1.4  compiler_4.0.3  
 [5] pillar_1.7.0     later_1.3.0      git2r_0.30.1     tools_4.0.3     
 [9] getPass_0.2-2    digest_0.6.29    jsonlite_1.8.0   evaluate_0.15   
[13] tibble_3.1.6     lifecycle_1.0.1  pkgconfig_2.0.3  rlang_1.0.4     
[17] DBI_1.1.2        cli_3.3.0        rstudioapi_0.13  yaml_2.3.5      
[21] xfun_0.30        fastmap_1.1.0    httr_1.4.2       stringr_1.4.0   
[25] knitr_1.38       generics_0.1.2   sass_0.4.1       fs_1.5.2        
[29] vctrs_0.4.1      tidyselect_1.1.2 rprojroot_2.0.3  glue_1.6.2      
[33] R6_2.5.1         processx_3.5.3   fansi_1.0.3      rmarkdown_2.16  
[37] purrr_0.3.4      callr_3.7.0      magrittr_2.0.3   whisker_0.4     
[41] ps_1.6.0         promises_1.2.0.1 htmltools_0.5.2  ellipsis_0.3.2  
[45] assertthat_0.2.1 httpuv_1.6.5     utf8_1.2.2       stringi_1.7.6   
[49] crayon_1.5.1