Last updated: 2019-12-03

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Knit directory: bentsen-rausch-2019/

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Project overview

A single intracerebral ventricular injection of FGF1 induces permanent normalization of blood glucose levels in multiple rodent models of type 2 diabetes. To understand the effects of FGF1 and identify cell-type specific responses key to blood glucose normalization, we performed multiple high throughput transcriptomic analyses at different timepoints in cohorts of male ob/ob mice.


Experimental Design

All the results and plots presented in the pages below should be reproduceible on your computer.

Increased sampling 5 days post treatment confirms findings and reveals novel responses

  1. Astrocytes and oligodendrocytes are the most responsive cell types at day 5

  2. WGCNA on astrocytes confirms snRNAseq findings

#3. Oligodendrocyte label transfer from Castelo-Branco et. al.

  1. Tanycytes activate specific gene programs across the 3rd ventricle

  2. FGF1 increases a pool of differentiating oligodendrocytes

#5. Tanycyte label transfer from Campbell et al.

Bulk RNA-seq reveals chronic effects of FGF1

  1. FGF1 increases synaptic plasticity up to 42 days post treatment

Credits

This project was led by [Dylan Rausch] (https://twitter.com/dylanrausch) and [Marie Bentsen] (https://cbmr.ku.dk/staff_overview/?pure=en%2Fpersons%2Fmarie-aare-bentsen(1f862ab6-010b-422f-b1aa-61b4a9557aa1)%2Fpublications.html) at the University of Copenhagen NNF CBMR (https://cbmr.ku.dk/) and University of Washington (https://endocrinology.uw.edu/)