Last updated: 2019-10-28

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Knit directory: fgf_alldata/

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#Project overview

A single ICV injection of FGF1 induces permanent normalization of blood glucose levels in multiple models of type 2 diabetes. To understand the effects of FGF1 and identify cell-type specific responses key to blood glucose normalization, we performed multiple high throughput transcriptomic analyses at different timepoints in cohorts of male ob/ob mice.


Experimental Design

All the results and plots presented in the pages below should be reproduceible on your computer.

##These are the results of analyses of neuronal populations in snRNA-seq data.

  1. Preparation of neuronal data for downstream analysis

  2. Identification of responsive neuronal populations

  3. Analysis of Agrp neurons

##These are the results of my analyses of glial cells from snRNA-seq data.

  1. Identification of responsive glial populations and there similarities

  2. WGCNA performed on macroglia reveals common processes across cell types

  3. WGCNA on astrocytes reveals biphasic activation

##These are the results of my whole cell analyses.

  1. Identification of responsive populations and there similarities

  2. WGCNA performed on astrocytes and overlaps between modules identified in snRNAseq
  3. Oligodendrocyte label transfer from Castelo-Branco et. al.

  4. Oligodendrocyte pseudotime analysis

  5. Tanycyte label transfer from Campbell et al.

  6. Tanycyte pseudoventricle analysis