Last updated: 2021-05-23

Checks: 1 1

Knit directory: wildlife-bacteria/

This reproducible R Markdown analysis was created with workflowr (version 1.6.2). The Checks tab describes the reproducibility checks that were applied when the results were created. The Past versions tab lists the development history.


The R Markdown file has unstaged changes. To know which version of the R Markdown file created these results, you’ll want to first commit it to the Git repo. If you’re still working on the analysis, you can ignore this warning. When you’re finished, you can run wflow_publish to commit the R Markdown file and build the HTML.

Great! You are using Git for version control. Tracking code development and connecting the code version to the results is critical for reproducibility.

The results in this page were generated with repository version 9ecc095. See the Past versions tab to see a history of the changes made to the R Markdown and HTML files.

Note that you need to be careful to ensure that all relevant files for the analysis have been committed to Git prior to generating the results (you can use wflow_publish or wflow_git_commit). workflowr only checks the R Markdown file, but you know if there are other scripts or data files that it depends on. Below is the status of the Git repository when the results were generated:


Ignored files:
    Ignored:    .DS_Store
    Ignored:    .Rhistory
    Ignored:    .Rproj.user/
    Ignored:    analysis/.DS_Store
    Ignored:    data/.DS_Store
    Ignored:    output/.DS_Store
    Ignored:    output/plots/.DS_Store
    Ignored:    output/plots/QC/.DS_Store
    Ignored:    output/plots/boxplots_select_taxa/.DS_Store
    Ignored:    output/plots/heatmaps/.DS_Store
    Ignored:    output/plots/maps/.DS_Store
    Ignored:    output/plots/tax_prev_abund/.DS_Store

Untracked files:
    Untracked:  NCBI_data/
    Untracked:  analysis/microbiome-viz-extra.Rmd
    Untracked:  analysis/phylogeny.Rmd
    Untracked:  analysis/tois.Rmd
    Untracked:  data/dada2/
    Untracked:  data/dada2_tois/
    Untracked:  data/taxa_trees/
    Untracked:  data/tmp/
    Untracked:  output/beta-div-statistics.txt
    Untracked:  output/supp_table_pos.xlsx
    Untracked:  tmp/

Unstaged changes:
    Modified:   README.md
    Modified:   analysis/_site.yml
    Modified:   analysis/index.Rmd
    Modified:   analysis/microbiome-viz.Rmd
    Modified:   analysis/phyloseq.Rmd

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These are the previous versions of the repository in which changes were made to the R Markdown (analysis/index.Rmd) and HTML (docs/index.html) files. If you’ve configured a remote Git repository (see ?wflow_git_remote), click on the hyperlinks in the table below to view the files as they were in that past version.

File Version Author Date Message
html a69dea3 siobhon-egan 2021-04-24 Build site.
Rmd 6ebcc5d siobhon-egan 2021-04-24 updates
html 6ebcc5d siobhon-egan 2021-04-24 updates
html a0173d4 siobhon-egan 2021-02-26 Build site.
Rmd d502188 siobhon-egan 2021-02-26 microbiome visualization
html 7aafdac siobhon-egan 2021-02-26 Build site.
Rmd 0e683ca siobhon-egan 2021-02-26 Publish the initial files for myproject
Rmd 681fc13 siobhon-egan 2021-01-29 Start workflowr project.

In the spirit of reproducibility this project website is created that outlines analysis done for the following publication:

The bacterial biome of ticks and their wildlife hosts at the sylvatic urban interface.

Authors: Siobhon L. Egan, Casey L. Taylor, Peter B. Banks, Amy S. Northover, Liisa A. Ahlstrom, Una M. Ryan, Peter J. Irwin, Charlotte L. Oskam.

This publication is also a chpater of my PhD Thesis 2021

Data availbility

Raw Illumina MiSeq data is available at NCBI SRA project XXXXX

Nucleotide sequence data available on Genbank

Anaplasmataceae (long 16S rRNA)
MW633160--MW633167

Borrelia 16S rRNA
MW633074

Bartonella ITS
MW633076--MW633082

ASVs for taxa of interest
XXXXXX-XXXXXX

Supporting data

Data used but not directly produced for this manuscript.

Nucleotide data for identification of ticks
MW665133–MW665150

Directory information

Worlflowr commands

To build/view webiste locally
wflow_build() and wflow_view()

Status of workflow
wflow_status()

Publish website pages
wflow_publish(c("analysis/index.Rmd", "analysis/first-analysis.Rmd"), "Add my first analysis")

Push website to github
wflow_git_push()