• Diagnostics and Quality Control Tools
  • ASEReadCounter
  • AnalyzeCovariates
  • CallableLoci
  • CheckPileup
  • CompareCallableLoci
  • ContEst
  • CountBases
  • CountIntervals
  • CountLoci
  • CountMales
  • CountRODs
  • CountRODsByRef
  • CountReadEvents
  • CountReads
  • CountTerminusEvent
  • DepthOfCoverage
  • DiagnoseTargets
  • DiffObjects
  • ErrorRatePerCycle
  • FastaStats
  • FindCoveredIntervals
  • FlagStat
  • GCContentByInterval
  • GatherBqsrReports
  • Pileup
  • PrintRODs
  • QualifyMissingIntervals
  • ReadClippingStats
  • ReadGroupProperties
  • ReadLengthDistribution
  • SimulateReadsForVariants
  • Sequence Data Processing Tools
  • BaseRecalibrator
  • ClipReads
  • IndelRealigner
  • LeftAlignIndels
  • PrintReads
  • RealignerTargetCreator
  • SplitNCigarReads
  • SplitSamFile
  • Variant Discovery Tools
  • ApplyRecalibration
  • CalculateGenotypePosteriors
  • GATKPaperGenotyper
  • GenotypeGVCFs
  • HaplotypeCaller
  • MuTect2
  • RegenotypeVariants
  • UnifiedGenotyper
  • VariantRecalibrator
  • Variant Evaluation Tools
  • GenotypeConcordance
  • ValidateVariants
  • VariantEval
  • VariantFiltration
  • Variant Manipulation Tools
  • CatVariants
  • CombineGVCFs
  • CombineVariants
  • HaplotypeResolver
  • LeftAlignAndTrimVariants
  • PhaseByTransmission
  • RandomlySplitVariants
  • ReadBackedPhasing
  • SelectHeaders
  • SelectVariants
  • ValidationSiteSelector
  • VariantAnnotator
  • VariantsToAllelicPrimitives
  • VariantsToBinaryPed
  • VariantsToTable
  • VariantsToVCF

  • Annotation Modules
  • AS_BaseQualityRankSumTest
  • AS_FisherStrand
  • AS_InbreedingCoeff
  • AS_InsertSizeRankSum
  • AS_MQMateRankSumTest
  • AS_MappingQualityRankSumTest
  • AS_QualByDepth
  • AS_RMSMappingQuality
  • AS_ReadPosRankSumTest
  • AS_StrandOddsRatio
  • AlleleBalance
  • AlleleBalanceBySample
  • AlleleCountBySample
  • BaseCounts
  • BaseCountsBySample
  • BaseQualityRankSumTest
  • BaseQualitySumPerAlleleBySample
  • ChromosomeCounts
  • ClippingRankSumTest
  • ClusteredReadPosition
  • Coverage
  • DepthPerAlleleBySample
  • DepthPerSampleHC
  • ExcessHet
  • FisherStrand
  • FractionInformativeReads
  • GCContent
  • GenotypeSummaries
  • HaplotypeScore
  • HardyWeinberg
  • HomopolymerRun
  • InbreedingCoeff
  • LikelihoodRankSumTest
  • LowMQ
  • MVLikelihoodRatio
  • MappingQualityRankSumTest
  • MappingQualityZero
  • MappingQualityZeroBySample
  • NBaseCount
  • OxoGReadCounts
  • PossibleDeNovo
  • QualByDepth
  • RMSMappingQuality
  • ReadPosRankSumTest
  • SampleList
  • SnpEff
  • SpanningDeletions
  • StrandAlleleCountsBySample
  • StrandBiasBySample
  • StrandOddsRatio
  • TandemRepeatAnnotator
  • TransmissionDisequilibriumTest
  • VariantType
  • Read Filters
  • BadCigarFilter
  • BadMateFilter
  • CountingFilteringIterator.CountingReadFilter
  • DuplicateReadFilter
  • FailsVendorQualityCheckFilter
  • HCMappingQualityFilter
  • LibraryReadFilter
  • MalformedReadFilter
  • MappingQualityFilter
  • MappingQualityUnavailableFilter
  • MappingQualityZeroFilter
  • MateSameStrandFilter
  • MaxInsertSizeFilter
  • MissingReadGroupFilter
  • NoOriginalQualityScoresFilter
  • NotPrimaryAlignmentFilter
  • OverclippedReadFilter
  • Platform454Filter
  • PlatformFilter
  • PlatformUnitFilter
  • ReadGroupBlackListFilter
  • ReadLengthFilter
  • ReadNameFilter
  • ReadStrandFilter
  • ReassignMappingQualityFilter
  • ReassignOneMappingQualityFilter
  • ReassignOriginalMQAfterIndelRealignmentFilter
  • SampleFilter
  • SingleReadGroupFilter
  • UnmappedReadFilter
  • Resource File Codecs
  • BeagleCodec
  • BedTableCodec
  • RawHapMapCodec
  • RefSeqCodec
  • SAMPileupCodec
  • SAMReadCodec
  • TableCodec

  • Reference Utilities
  • FastaAlternateReferenceMaker
  • FastaReferenceMaker
  • QCRef
  • Showing docs for version 3.7-0


    QCRef

    Quality control for the reference fasta

    Category Reference Utilities

    Traversal LocusWalker

    PartitionBy LOCUS


    Overview

    Input

    One reference file only. And optionally -L intervals

    Output

    If the reference is fully valid, the run will complete successfully. If not, an error message will be produced at the site where the program encountered a problem.

    Usage example

     java -jar GenomeAnalysisTK.jar \
       -T QCRef \
       -R reference.fasta
     

    Additional Information

    Read filters

    These Read Filters are automatically applied to the data by the Engine before processing by QCRef.

    Downsampling settings

    This tool applies the following downsampling settings by default.


    Command-line Arguments

    Engine arguments

    All tools inherit arguments from the GATK Engine' "CommandLineGATK" argument collection, which can be used to modify various aspects of the tool's function. For example, the -L argument directs the GATK engine to restrict processing to specific genomic intervals; or the -rf argument allows you to apply certain read filters to exclude some of the data from the analysis.

    QCRef specific arguments

    This table summarizes the command-line arguments that are specific to this tool. For more details on each argument, see the list further down below the table or click on an argument name to jump directly to that entry in the list.

    Argument name(s) Default value Summary
    Optional Outputs
    --out
     -o
    stdout An output file created by the walker. Will overwrite contents if file exists

    Argument details

    Arguments in this list are specific to this tool. Keep in mind that other arguments are available that are shared with other tools (e.g. command-line GATK arguments); see Inherited arguments above.


    --out / -o

    An output file created by the walker. Will overwrite contents if file exists

    PrintStream  stdout